MD17G1230100.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002221
Physical Location & Seq
Forward (+)
27813167 .. 27817007
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1230100.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGGAGGGGTCTCAGGGTGGTTCTGGTGGCGCACCGGCGCCTTTTCTGACCAAAACATATGACTTGGTGGATGATCCTTCTTCCAACCACATGGTGTCCTGGACTGAGAGTGGTTCTAGTTTCGTAGTTTGGGATCCCACCGAGTTCGCCAAGGAAATGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTCGTGAGGCAGCTTAATACATATGGGTTTAGAAAGATTGATCCTGAACAGTGGGAGTTTGCAAATGAGGAATTTTTAAGAGGAGGAAGACATCTGCTGAAGAATATTCACCGCCGCAAGCCAATTCACAGTCATTCCATGCAGAATCACGAGTATACAGTTGCTTTAAGTGATACAGAGAGAGAAGAATATGAGAAGAAAATCAGTAGACTGAATCATGATAAAAGCTTGCTTGAGTTGGAGCTACAGCGGCATCAAAGAGAGAATCAGGAGTTTGAATTTCAAGTACAGATATTACGAGAACAACTGCAGAACATGGAGAATCGGCAGAAGCAATATACGGCCTTCTTGGCTCAACTAGTGCAGAAACCAGGATTTGCTTCTGTACTTGTGCAGCAGTCAGAAATCCATAGCAAAAAGAGAAGATTACTGAATTCAAACAACTTTCCTGATGACTTTGGTATGGAAGGTTTGAATCTGAATCCGCAGAAAGAAAATTTGGGATCAATTTCAACTCCCATAATAAAGTTGGACCAGCTTGAGACGATGGAGTCTTCCTTAAACTTTTGGGAAGATTTTCTACATGGGATTGGGGAAGCAATGCCCGAAGATGTAAATGATATTGGCCCGTTGTCTCAGGCCTCTCCTATCATTGTTACCGAAACACAAGACACCAGCTTGAATAGCAGGCCTTGCTCACCTAGATCACATTTATCTTCACCGAATTCAATGAATGCTCATTCATTCCCAGAGGTGGCAGCTGGGTCTGCAAATATTCTTGACATCCTTTCTATAACATCAATGTTCCCCACTGTTGATTTCAGGTCTAAATCTTCAGGAATCGATATGAACTCTAAGCCCGACAGTGCCCCTGCACTTGCCAAAGAAATGGTAGTGGAACTGGAAGTGAAAAATGCCGAGCCTACCAAGGCAAATGATGTATTTTGGGAACAATGCCTAACAGAGACCCCTGGTTTAGCTGATGCGCTGGAAGTACACTCAGAAAGAAGGGATGGCGATGGCAAAGCGAGCGATGCCAATCCAGCTATCCAGAAAAAGCACTGGTGGAACACAGATAATGTCGATAACTTCACCAACCAAATAGGGCGCCTCACTCCAGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

441

Amino Acids

49.84

Weight (kDa)

5.21

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 15 - 104 8.4e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 37, 1302
AccI GTMKAC 2 cut(s) 350, 403
AciI CCGC 4 cut(s) 307, 310, 445, 680
AclWI GGATC 5 cut(s) 68, 128, 141, 230, 706
AcsI RAATTY 5 cut(s) 266, 473, 628, 691, 919
AcuI CTGAAG 2 cut(s) 314, 1014
AcyI GRCGYC 2 cut(s) 38, 1303
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 3 cut(s) 483, 582, 1191
AfiI CCNNNNNNNGG 1 cut(s) 949
AgsI TTSAA 8 cut(s) 175, 473, 479, 633, 670, 708, 877, 924
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 3 cut(s) 98, 565, 1165
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AluBI AGCT 9 cut(s) 208, 423, 439, 733, 873, 956, 1175, 1241, 1316
AluI AGCT 9 cut(s) 208, 423, 439, 733, 873, 956, 1175, 1241, 1316
Alw26I GTCTC 4 cut(s) 15, 731, 834, 1154
AlwI GGATC 5 cut(s) 68, 128, 141, 230, 706
AoxI GGCC 4 cut(s) 537, 820, 834, 884
ApeKI GCWGC 3 cut(s) 205, 589, 953
ApoI RAATTY 5 cut(s) 266, 473, 628, 691, 919
Asp700I GAANNNNTTC 2 cut(s) 159, 771
AspLEI GCGC 4 cut(s) 32, 40, 1183, 1305
AspS9I GGNCC 2 cut(s) 727, 821
AsuHPI GGTGA 4 cut(s) 296, 885, 906, 1279
AvaII GGWCC 1 cut(s) 727
BaeGI GKGCMC 1 cut(s) 1066
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 37, 1302
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 289, 741
BbvI GCAGC 3 cut(s) 217, 601, 965
BccI CCATC 3 cut(s) 736, 1202, 1208
BceAI ACGGC 1 cut(s) 552
BciT130I CCWGG 3 cut(s) 100, 567, 1167
BcoDI GTCTC 4 cut(s) 15, 731, 834, 1154
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 3 cut(s) 117, 554, 897
BfmI CTRYAG 2 cut(s) 440, 503
BfoI RGCGCY 2 cut(s) 41, 1306
BglI GCCNNNNNGGC 1 cut(s) 545
BisI GCNGC 5 cut(s) 206, 310, 446, 590, 954
BlsI GCNGC 5 cut(s) 207, 311, 447, 591, 955
Bme1390I CCNGG 3 cut(s) 100, 567, 1167
Bme18I GGWCC 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 727, 821
BmiI GGNNCC 3 cut(s) 39, 135, 1304
BmrFI CCNGG 3 cut(s) 100, 567, 1167
BmsI GCATC 3 cut(s) 457, 1168, 1219
BpiI GAAGAC 2 cut(s) 289, 741
BpmI CTGGAG 1 cut(s) 1296
BpuEI CTTGAG 2 cut(s) 449, 755
Bsa29I ATCGAT 1 cut(s) 1038
BsaBI GATNNNNATC 1 cut(s) 1233
BsaHI GRCGYC 2 cut(s) 38, 1303
BsaI GGTCTC 2 cut(s) 15, 1154
BsaJI CCNNGG 3 cut(s) 150, 1122, 1165
Bsc4I CCNNNNNNNGG 1 cut(s) 949
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 1101, 1262
Bse3DI GCAATG 1 cut(s) 801
Bse8I GATNNNNATC 1 cut(s) 1233
BseBI CCWGG 3 cut(s) 100, 567, 1167
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 3 cut(s) 150, 1122, 1165
BseGI GGATG 3 cut(s) 76, 978, 1213
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 1 cut(s) 949
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 4 cut(s) 26, 96, 845, 1209
BseNI ACTGG 2 cut(s) 1101, 1262
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 1066
BseXI GCAGC 3 cut(s) 217, 601, 965
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 3 cut(s) 578, 608, 1053
BshFI GGCC 4 cut(s) 539, 822, 836, 886
BshNI GGYRCC 2 cut(s) 37, 1302
BshVI ATCGAT 1 cut(s) 1038
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 949
BsmAI GTCTC 4 cut(s) 15, 731, 834, 1154
BsmBI CGTCTC 1 cut(s) 731
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 4 cut(s) 539, 822, 836, 886
Bso31I GGTCTC 2 cut(s) 15, 1154
Bsp1286I GDGCHC 1 cut(s) 1066
Bsp143I GATC 5 cut(s) 73, 133, 235, 698, 899
BspACI CCGC 4 cut(s) 307, 310, 445, 680
BspANI GGCC 4 cut(s) 539, 822, 836, 886
BspCNI CTCAG 4 cut(s) 25, 97, 844, 1208
BspDI ATCGAT 1 cut(s) 1038
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 39, 135, 1304
BspMAI CTGCAG 1 cut(s) 507
BspPI GGATC 5 cut(s) 68, 128, 141, 230, 706
BspT107I GGYRCC 2 cut(s) 37, 1302
BspTNI GGTCTC 2 cut(s) 15, 1154
BsrDI GCAATG 1 cut(s) 801
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 2 cut(s) 1101, 1262
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 150, 1122, 1165
BssMI GATC 5 cut(s) 73, 133, 235, 698, 899
BssNAI GTATAC 1 cut(s) 351
BssNI GRCGYC 2 cut(s) 38, 1303
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 150, 1122
Bst1107I GTATAC 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 3 cut(s) 100, 567, 1167
Bst4CI ACNGT 5 cut(s) 246, 326, 355, 1009, 1061
BstACI GRCGYC 2 cut(s) 38, 1303
BstAPI GCANNNNNTGC 1 cut(s) 888
BstC8I GCNNGC 4 cut(s) 314, 425, 884, 1225
BstDEI CTNAG 5 cut(s) 12, 105, 831, 1050, 1195
BstF5I GGATG 3 cut(s) 76, 978, 1213
BstH2I RGCGCY 2 cut(s) 41, 1306
BstHHI GCGC 4 cut(s) 32, 40, 1183, 1305
BstKTI GATC 5 cut(s) 76, 136, 238, 701, 902
BstMAI GTCTC 4 cut(s) 15, 731, 834, 1154
BstMBI GATC 5 cut(s) 73, 133, 235, 698, 899
BstMWI GCNNNNNNNGC 8 cut(s) 445, 545, 879, 888, 962, 1224, 1229, 1238
BstNI CCWGG 3 cut(s) 100, 567, 1167
BstSCI CCNGG 3 cut(s) 98, 565, 1165
BstSFI CTRYAG 2 cut(s) 440, 503
BstSLI GKGCMC 1 cut(s) 1066
BstV1I GCAGC 3 cut(s) 217, 601, 965
BstV2I GAAGAC 2 cut(s) 289, 741
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 2 cut(s) 91, 1084
BstYI RGATCY 1 cut(s) 133
BstZ17I GTATAC 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 1038
BsuRI GGCC 4 cut(s) 539, 822, 836, 886
BsuTUI ATCGAT 1 cut(s) 1038
BtgZI GCGATG 2 cut(s) 1227, 1242
BtsCI GGATG 3 cut(s) 76, 978, 1213
BtsIMutI CAGTG 4 cut(s) 251, 1005, 1066, 1255
Cac8I GCNNGC 4 cut(s) 314, 425, 884, 1225
CciI TCATGA 1 cut(s) 412
CfoI GCGC 4 cut(s) 32, 40, 1183, 1305
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 727, 821
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 3 cut(s) 482, 581, 1190
CviAII CATG 6 cut(s) 91, 166, 334, 413, 511, 779
CviQI GTAC 3 cut(s) 482, 581, 1190
DdeI CTNAG 5 cut(s) 12, 105, 831, 1050, 1195
DinI GGCGCC 2 cut(s) 39, 1304
DpnI GATC 5 cut(s) 75, 135, 237, 700, 901
DpnII GATC 5 cut(s) 73, 133, 235, 698, 899
DraIII CACNNNGTG 1 cut(s) 94
DrdI GACNNNNNNGTC 1 cut(s) 745
DseDI GACNNNNNNGTC 1 cut(s) 745
Eco130I CCWWGG 2 cut(s) 150, 1122
Eco147I AGGCCT 2 cut(s) 836, 886
Eco31I GGTCTC 2 cut(s) 15, 1154
Eco47I GGWCC 1 cut(s) 727
Eco57I CTGAAG 2 cut(s) 314, 1014
EcoRI GAATTC 2 cut(s) 628, 919
EcoRII CCWGG 3 cut(s) 98, 565, 1165
EcoT14I CCWWGG 2 cut(s) 150, 1122
EgeI GGCGCC 2 cut(s) 39, 1304
EheI GGCGCC 2 cut(s) 39, 1304
ErhI CCWWGG 2 cut(s) 150, 1122
Esp3I CGTCTC 1 cut(s) 731
FaeI CATG 6 cut(s) 94, 169, 337, 416, 514, 782
FatI CATG 6 cut(s) 90, 165, 333, 412, 510, 778
FauNDI CATATG 2 cut(s) 58, 217
FblI GTMKAC 2 cut(s) 350, 403
Fnu4HI GCNGC 5 cut(s) 206, 310, 446, 590, 954
FokI GGATG 3 cut(s) 83, 965, 1220
Fsp4HI GCNGC 5 cut(s) 206, 310, 446, 590, 954
FspBI CTAG 3 cut(s) 117, 554, 897
GlaI GCGC 4 cut(s) 31, 39, 1182, 1304
GluI GCNGC 5 cut(s) 206, 310, 446, 590, 954
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 1296
HaeII RGCGCY 2 cut(s) 41, 1306
HaeIII GGCC 4 cut(s) 539, 822, 836, 886
HapII CCGG 1 cut(s) 35
HhaI GCGC 4 cut(s) 32, 40, 1183, 1305
Hin1I GRCGYC 2 cut(s) 38, 1303
Hin1II CATG 6 cut(s) 94, 169, 337, 416, 514, 782
Hin6I GCGC 4 cut(s) 30, 38, 1181, 1303
HinP1I GCGC 4 cut(s) 30, 38, 1181, 1303
HindIII AAGCTT 1 cut(s) 421
HinfI GANTC 8 cut(s) 340, 409, 460, 517, 670, 676, 746, 1035
HpaII CCGG 1 cut(s) 35
HphI GGTGA 4 cut(s) 296, 885, 906, 1279
Hpy166II GTNNAC 3 cut(s) 351, 404, 1192
Hpy188I TCNGA 4 cut(s) 48, 598, 675, 1198
Hpy188III TCNNGA 9 cut(s) 199, 239, 344, 413, 464, 644, 974, 1032, 1246
Hpy8I GTNNAC 3 cut(s) 351, 404, 1192
HpyAV CCTTC 4 cut(s) 87, 550, 656, 1197
HpyCH4III ACNGT 5 cut(s) 246, 326, 355, 1009, 1061
HpyCH4V TGCA 7 cut(s) 257, 337, 505, 559, 589, 965, 1070
HpyF10VI GCNNNNNNNGC 8 cut(s) 445, 545, 879, 888, 962, 1224, 1229, 1238
HpyF3I CTNAG 5 cut(s) 12, 105, 831, 1050, 1195
Hsp92I GRCGYC 2 cut(s) 38, 1303
Hsp92II CATG 6 cut(s) 94, 169, 337, 416, 514, 782
HspAI GCGC 4 cut(s) 30, 38, 1181, 1303
KasI GGCGCC 2 cut(s) 37, 1302
Kzo9I GATC 5 cut(s) 73, 133, 235, 698, 899
LmnI GCTCC 1 cut(s) 436
Lsp1109I GCAGC 3 cut(s) 217, 601, 965
LweI GCATC 3 cut(s) 457, 1168, 1219
MaeI CTAG 3 cut(s) 117, 554, 897
MaeIII GTNAC 1 cut(s) 850
MalI GATC 5 cut(s) 75, 135, 237, 700, 901
MboI GATC 5 cut(s) 73, 133, 235, 698, 899
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 1066
MluCI AATT 8 cut(s) 184, 266, 318, 473, 628, 691, 702, 919
Mly113I GGCGCC 2 cut(s) 38, 1303
MlyI GAGTC 1 cut(s) 755
MmeI TCCRAC 3 cut(s) 108, 414, 705
MnlI CCTC 7 cut(s) 195, 256, 269, 272, 847, 940, 1316
MroXI GAANNNNTTC 2 cut(s) 159, 771
MseI TTAA 4 cut(s) 210, 272, 362, 755
MspA1I CMGCKG 2 cut(s) 445, 956
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 3 cut(s) 100, 567, 1167
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 3 cut(s) 100, 567, 1167
MwoI GCNNNNNNNGC 8 cut(s) 445, 545, 879, 888, 962, 1224, 1229, 1238
NarI GGCGCC 2 cut(s) 38, 1303
NdeI CATATG 2 cut(s) 58, 217
NdeII GATC 5 cut(s) 73, 133, 235, 698, 899
NlaIII CATG 6 cut(s) 94, 169, 337, 416, 514, 782
NlaIV GGNNCC 3 cut(s) 39, 135, 1304
NmeAIII GCCGAG 1 cut(s) 1138
PagI TCATGA 1 cut(s) 412
PceI AGGCCT 2 cut(s) 836, 886
PctI GAATGC 1 cut(s) 934
PdmI GAANNNNTTC 2 cut(s) 159, 771
PfeI GAWTC 7 cut(s) 340, 409, 460, 517, 670, 676, 1035
PfoI TCCNGGA 1 cut(s) 98
PkrI GCNGC 5 cut(s) 207, 311, 447, 591, 955
PleI GAGTC 1 cut(s) 754
PluTI GGCGCC 2 cut(s) 41, 1306
PpsI GAGTC 1 cut(s) 754
Psp6I CCWGG 3 cut(s) 98, 565, 1165
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 3 cut(s) 98, 565, 1165
PspN4I GGNNCC 3 cut(s) 39, 135, 1304
PspPI GGNCC 2 cut(s) 727, 821
PstI CTGCAG 1 cut(s) 507
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 3 cut(s) 483, 582, 1191
RsaNI GTAC 3 cut(s) 482, 581, 1190
SaqAI TTAA 4 cut(s) 210, 272, 362, 755
SatI GCNGC 5 cut(s) 206, 310, 446, 590, 954
Sau3AI GATC 5 cut(s) 73, 133, 235, 698, 899
Sau96I GGNCC 2 cut(s) 727, 821
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 3 cut(s) 100, 567, 1167
SduI GDGCHC 1 cut(s) 1066
SfaNI GCATC 3 cut(s) 457, 1168, 1219
SfcI CTRYAG 2 cut(s) 440, 503
SfoI GGCGCC 2 cut(s) 39, 1304
SgrAI CRCCGGYG 1 cut(s) 34
SinI GGWCC 1 cut(s) 727
SmlI CTYRAG 2 cut(s) 428, 734
SmoI CTYRAG 2 cut(s) 428, 734
SpeI ACTAGT 1 cut(s) 553
Sse9I AATT 8 cut(s) 184, 266, 318, 473, 628, 691, 702, 919
SseBI AGGCCT 2 cut(s) 836, 886
SsiI CCGC 4 cut(s) 307, 310, 445, 680
SspDI GGCGCC 2 cut(s) 37, 1302
SspI AATATT 2 cut(s) 301, 970
SspMI CTAG 3 cut(s) 117, 554, 897
StuI AGGCCT 2 cut(s) 836, 886
StyD4I CCNGG 3 cut(s) 98, 565, 1165
StyI CCWWGG 2 cut(s) 150, 1122
TaaI ACNGT 5 cut(s) 246, 326, 355, 1009, 1061
TaqI TCGA 3 cut(s) 191, 1038, 1278
TasI AATT 8 cut(s) 184, 266, 318, 473, 628, 691, 702, 919
TatI WGTACW 3 cut(s) 481, 580, 1189
TauI GCSGC 2 cut(s) 312, 448
TfiI GAWTC 7 cut(s) 340, 409, 460, 517, 670, 676, 1035
Tru1I TTAA 4 cut(s) 210, 272, 362, 755
Tru9I TTAA 4 cut(s) 210, 272, 362, 755
TscAI CASTG 4 cut(s) 251, 1012, 1066, 1262
TseI GCWGC 3 cut(s) 205, 589, 953
TspDTI ATGAA 3 cut(s) 927, 941, 1058
TspRI CASTG 4 cut(s) 251, 1012, 1066, 1262
VpaK11BI GGWCC 1 cut(s) 727
XapI RAATTY 5 cut(s) 266, 473, 628, 691, 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmiI GTMKAC 2 cut(s) 350, 403
XmnI GAANNNNTTC 2 cut(s) 159, 771
XspI CTAG 3 cut(s) 117, 554, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.