MD09G1233700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002221
Physical Location & Seq
Forward (+)
29363539 .. 29366926
3388 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1233700.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGTGTCTTGGAGTGAAACTGGTTCTAGTTTTGTAGTTTGGGATCCCACCGAGTTCGCCAAGGAAATGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTCGTGAGGCAGCTTAATACATATGGGTTTAGAAAGATTGATCCAGAACAGTGGGAGTTTGCAAATGAGGAATTCTTAAGAGGAGGAAGACATCTGCTGAAGAATATTCACCGCCGCAAGCCAATTCATAGTCATTCCTTGCAGAATCACGAGTATTCTTCAGTTCCTTTGACTGATACGGAGAGAGAAGAATATGAGAAGAAAATCAACAGACTGAATCATGATAAAAGCTTGCTTGAGTTGGAGGTACAACGGCATCAAAGAGAGAGTCAGGAGTTTGAATTTCAAATACAGATATTACGAGAACAACTGCAGAACATGGAGAATCGGCAGAGGCAATACACGGCTTTCCTGGCTCAACTAGTGCAGAAACCGGGATTTGCTTCGGTACTTGTGCAGCAGTCAGAAATCCATAGCAAAAAGAGAAGATTACTGAAATCGAACCACTTTCCCGATGACTTCGGTATGGAAGATTTGACTTTGAATCCGCAGAAAGAAACTTTGGGAATAAAGTTGGACCAGTTTGAGAAGATGGAGTCTTCCTTGAACTTTTGGGAAGATTTTCTACATGGGGTTGGGGAAGCGATGCCCGAAGAAGTAAATGATATTGGCCCATTGTCTCAGGCCTCTCCTATCATTGTTACAGAAATACAAGACACCAGCATGAATAGCAGGCCTTGCTCACCTAGATCGCATATATCTTCACCGAATTCAATGAATGCTCATTCCTTCCCAGAGGTGGCTGGGTCTGCAAATCTTTTTGACATCCTTGCTATAACATCAATGTGCCACACTGTTGATTTCAGGTCTAAATCTTCAGGAATTGATATGAACTCCAAGCCCGACGGTGCCCCTGCTCTTGATTCCTCAAAAGAAAATGTAGTACAAGTGAAAAATGCCGAGCCTGCCAAGGCAAATGATGTCTTTTGGGAACAATGCCTAACAGAGACTCCTGGTTTAGATGATGCGGCGGAAGTACAGTCAGAAAGAAGCGATGGCGATGGCGATGGCAAAGCGAGTGATGCCAATCCGGCTATTCGGAAGAAGCTTTGGTGGAACACAGATAATGTAGATAACTTTAGGAACCAAATAGGGCGCCTCACTCCAGCTACTAATTTTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

410

Amino Acids

46.92

Weight (kDa)

5.45

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 2 - 74 1.7e-25 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 953, 1200
AciI CCGC 5 cut(s) 217, 220, 593, 1073, 1076
AclWI GGATC 3 cut(s) 38, 51, 140
AcsI RAATTY 3 cut(s) 176, 386, 814
AcuI CTGAAG 3 cut(s) 224, 249, 906
AcyI GRCGYC 1 cut(s) 1201
AfaI GTAC 4 cut(s) 354, 495, 990, 1083
AfiI CCNNNNNNNGG 1 cut(s) 844
AflII CTTAAG 1 cut(s) 181
AgsI TTSAA 6 cut(s) 85, 386, 392, 589, 652, 819
AhlI ACTAGT 1 cut(s) 466
AjnI CCWGG 2 cut(s) 456, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
AluBI AGCT 4 cut(s) 118, 336, 1153, 1214
AluI AGCT 4 cut(s) 118, 336, 1153, 1214
Alw26I GTCTC 2 cut(s) 729, 1046
AlwI GGATC 3 cut(s) 38, 51, 140
AoxI GGCC 3 cut(s) 715, 729, 779
ApeKI GCWGC 2 cut(s) 115, 502
ApoI RAATTY 3 cut(s) 176, 386, 814
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
Asp700I GAANNNNTTC 2 cut(s) 69, 666
AspLEI GCGC 1 cut(s) 1203
AspS9I GGNCC 2 cut(s) 622, 716
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 3 cut(s) 206, 780, 801
AvaII GGWCC 1 cut(s) 622
BaeGI GKGCMC 1 cut(s) 958
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 2 cut(s) 953, 1200
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 199, 636
BbvI GCAGC 2 cut(s) 127, 514
BccI CCATC 4 cut(s) 631, 1094, 1100, 1106
BceAI ACGGC 2 cut(s) 374, 465
BciT130I CCWGG 2 cut(s) 458, 1059
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 2 cut(s) 729, 1046
BcuI ACTAGT 1 cut(s) 466
BfaI CTAG 3 cut(s) 27, 467, 792
BfmI CTRYAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 1204
BfrI CTTAAG 1 cut(s) 181
BglI GCCNNNNNGGC 1 cut(s) 1136
BisI GCNGC 4 cut(s) 116, 220, 503, 1074
BlsI GCNGC 4 cut(s) 117, 221, 504, 1075
Bme1390I CCNGG 3 cut(s) 458, 480, 1059
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 622, 716
BmiI GGNNCC 4 cut(s) 45, 955, 1190, 1202
BmrFI CCNGG 3 cut(s) 458, 480, 1059
BmsI GCATC 4 cut(s) 370, 681, 1060, 1117
BpiI GAAGAC 2 cut(s) 199, 636
BpmI CTGGAG 1 cut(s) 1194
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 1131
BsaHI GRCGYC 1 cut(s) 1201
BsaJI CCNNGG 2 cut(s) 60, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 1039, 1069
Bsc4I CCNNNNNNNGG 1 cut(s) 844
Bse1I ACTGG 2 cut(s) 25, 625
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 2 cut(s) 458, 1059
BseDI CCNNGG 2 cut(s) 60, 1014
BseGI GGATG 1 cut(s) 870
BseJI GATNNNNATC 1 cut(s) 1131
BseLI CCNNNNNNNGG 1 cut(s) 844
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 2 cut(s) 25, 625
BseRI GAGGAG 1 cut(s) 201
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 2 cut(s) 127, 514
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 2 cut(s) 491, 521
BshFI GGCC 3 cut(s) 717, 731, 781
BshNI GGYRCC 2 cut(s) 953, 1200
BsiSI CCGG 2 cut(s) 479, 1136
BslI CCNNNNNNNGG 1 cut(s) 844
BsmAI GTCTC 2 cut(s) 729, 1046
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 3 cut(s) 717, 731, 781
Bsp1286I GDGCHC 1 cut(s) 958
Bsp143I GATC 3 cut(s) 43, 145, 794
BspACI CCGC 5 cut(s) 217, 220, 593, 1073, 1076
BspANI GGCC 3 cut(s) 717, 731, 781
BspCNI CTCAG 1 cut(s) 739
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 4 cut(s) 45, 955, 1190, 1202
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 3 cut(s) 38, 51, 140
BspT107I GGYRCC 2 cut(s) 953, 1200
BspTI CTTAAG 1 cut(s) 181
BsrI ACTGG 2 cut(s) 25, 625
BssECI CCNNGG 2 cut(s) 60, 1014
BssMI GATC 3 cut(s) 43, 145, 794
BssNI GRCGYC 1 cut(s) 1201
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 2 cut(s) 60, 1014
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 2 cut(s) 458, 1059
Bst4CI ACNGT 4 cut(s) 156, 901, 953, 1086
BstACI GRCGYC 1 cut(s) 1201
BstAFI CTTAAG 1 cut(s) 181
BstAPI GCANNNNNTGC 1 cut(s) 783
BstC8I GCNNGC 4 cut(s) 224, 338, 779, 1011
BstDEI CTNAG 1 cut(s) 726
BstF5I GGATG 1 cut(s) 870
BstH2I RGCGCY 1 cut(s) 1204
BstHHI GCGC 1 cut(s) 1203
BstKTI GATC 3 cut(s) 46, 148, 797
BstMAI GTCTC 2 cut(s) 729, 1046
BstMBI GATC 3 cut(s) 43, 145, 794
BstMWI GCNNNNNNNGC 7 cut(s) 458, 774, 783, 854, 1010, 1127, 1136
BstNI CCWGG 2 cut(s) 458, 1059
BstSCI CCNGG 3 cut(s) 456, 478, 1057
BstSFI CTRYAG 1 cut(s) 416
BstSLI GKGCMC 1 cut(s) 958
BstV1I GCAGC 2 cut(s) 127, 514
BstV2I GAAGAC 2 cut(s) 199, 636
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 3 cut(s) 717, 731, 781
BtgZI GCGATG 4 cut(s) 704, 1113, 1119, 1125
BtsCI GGATG 1 cut(s) 870
BtsIMutI CAGTG 2 cut(s) 161, 897
Cac8I GCNNGC 4 cut(s) 224, 338, 779, 1011
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 1203
Cfr13I GGNCC 2 cut(s) 622, 716
Csp6I GTAC 4 cut(s) 353, 494, 989, 1082
CviAII CATG 5 cut(s) 76, 326, 424, 674, 769
CviQI GTAC 4 cut(s) 353, 494, 989, 1082
DdeI CTNAG 1 cut(s) 726
DinI GGCGCC 1 cut(s) 1202
DpnI GATC 3 cut(s) 45, 147, 796
DpnII GATC 3 cut(s) 43, 145, 794
EciI GGCGGA 1 cut(s) 1091
Eco130I CCWWGG 2 cut(s) 60, 1014
Eco147I AGGCCT 2 cut(s) 731, 781
Eco47I GGWCC 1 cut(s) 622
Eco57I CTGAAG 3 cut(s) 224, 249, 906
EcoRI GAATTC 2 cut(s) 176, 814
EcoRII CCWGG 2 cut(s) 456, 1057
EcoT14I CCWWGG 2 cut(s) 60, 1014
EgeI GGCGCC 1 cut(s) 1202
EheI GGCGCC 1 cut(s) 1202
ErhI CCWWGG 2 cut(s) 60, 1014
FaeI CATG 5 cut(s) 79, 329, 427, 677, 772
FatI CATG 5 cut(s) 75, 325, 423, 673, 768
FauNDI CATATG 1 cut(s) 127
Fnu4HI GCNGC 4 cut(s) 116, 220, 503, 1074
FokI GGATG 1 cut(s) 857
Fsp4HI GCNGC 4 cut(s) 116, 220, 503, 1074
FspBI CTAG 3 cut(s) 27, 467, 792
GlaI GCGC 1 cut(s) 1202
GluI GCNGC 4 cut(s) 116, 220, 503, 1074
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 1194
HaeII RGCGCY 1 cut(s) 1204
HaeIII GGCC 3 cut(s) 717, 731, 781
HapII CCGG 2 cut(s) 479, 1136
HhaI GCGC 1 cut(s) 1203
Hin1I GRCGYC 1 cut(s) 1201
Hin1II CATG 5 cut(s) 79, 329, 427, 677, 772
Hin6I GCGC 1 cut(s) 1201
HinP1I GCGC 1 cut(s) 1201
HindIII AAGCTT 2 cut(s) 334, 1151
HinfI GANTC 8 cut(s) 250, 322, 373, 430, 589, 641, 968, 1054
HpaII CCGG 2 cut(s) 479, 1136
HphI GGTGA 3 cut(s) 206, 780, 801
Hpy188I TCNGA 3 cut(s) 511, 1090, 1146
Hpy188III TCNNGA 8 cut(s) 109, 149, 254, 326, 377, 557, 924, 965
Hpy99I CGWCG 1 cut(s) 953
HpyAV CCTTC 1 cut(s) 844
HpyCH4III ACNGT 4 cut(s) 156, 901, 953, 1086
HpyCH4V TGCA 6 cut(s) 167, 247, 418, 472, 502, 857
HpyF10VI GCNNNNNNNGC 7 cut(s) 458, 774, 783, 854, 1010, 1127, 1136
HpyF3I CTNAG 1 cut(s) 726
Hsp92I GRCGYC 1 cut(s) 1201
Hsp92II CATG 5 cut(s) 79, 329, 427, 677, 772
HspAI GCGC 1 cut(s) 1201
KasI GGCGCC 1 cut(s) 1200
Kzo9I GATC 3 cut(s) 43, 145, 794
Lsp1109I GCAGC 2 cut(s) 127, 514
LweI GCATC 4 cut(s) 370, 681, 1060, 1117
MaeI CTAG 3 cut(s) 27, 467, 792
MaeIII GTNAC 1 cut(s) 745
MalI GATC 3 cut(s) 45, 147, 796
MboI GATC 3 cut(s) 43, 145, 794
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 1 cut(s) 958
MluCI AATT 7 cut(s) 94, 176, 228, 386, 814, 927, 1219
Mly113I GGCGCC 1 cut(s) 1201
MlyI GAGTC 3 cut(s) 382, 650, 1048
MmeI TCCRAC 2 cut(s) 327, 600
MroXI GAANNNNTTC 2 cut(s) 69, 666
MseI TTAA 2 cut(s) 120, 182
MslI CAYNNNNRTG 2 cut(s) 767, 889
MspCI CTTAAG 1 cut(s) 181
MspI CCGG 2 cut(s) 479, 1136
MspR9I CCNGG 3 cut(s) 458, 480, 1059
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 2 cut(s) 458, 1059
MwoI GCNNNNNNNGC 7 cut(s) 458, 774, 783, 854, 1010, 1127, 1136
NarI GGCGCC 1 cut(s) 1201
NciI CCSGG 1 cut(s) 480
NdeI CATATG 1 cut(s) 127
NdeII GATC 3 cut(s) 43, 145, 794
NlaIII CATG 5 cut(s) 79, 329, 427, 677, 772
NlaIV GGNNCC 4 cut(s) 45, 955, 1190, 1202
NmeAIII GCCGAG 1 cut(s) 1030
PagI TCATGA 1 cut(s) 325
PceI AGGCCT 2 cut(s) 731, 781
PctI GAATGC 1 cut(s) 829
PdmI GAANNNNTTC 2 cut(s) 69, 666
PfeI GAWTC 5 cut(s) 250, 322, 430, 589, 968
PkrI GCNGC 4 cut(s) 117, 221, 504, 1075
PleI GAGTC 3 cut(s) 381, 649, 1048
PluTI GGCGCC 1 cut(s) 1204
PpsI GAGTC 3 cut(s) 381, 649, 1048
Psp6I CCWGG 2 cut(s) 456, 1057
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 2 cut(s) 456, 1057
PspN4I GGNNCC 4 cut(s) 45, 955, 1190, 1202
PspPI GGNCC 2 cut(s) 622, 716
PstI CTGCAG 1 cut(s) 420
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 4 cut(s) 354, 495, 990, 1083
RsaNI GTAC 4 cut(s) 353, 494, 989, 1082
RseI CAYNNNNRTG 2 cut(s) 767, 889
SaqAI TTAA 2 cut(s) 120, 182
SatI GCNGC 4 cut(s) 116, 220, 503, 1074
Sau3AI GATC 3 cut(s) 43, 145, 794
Sau96I GGNCC 2 cut(s) 622, 716
SchI GAGTC 3 cut(s) 382, 650, 1048
ScrFI CCNGG 3 cut(s) 458, 480, 1059
SduI GDGCHC 1 cut(s) 958
SetI ASST 8 cut(s) 120, 338, 354, 793, 846, 914, 1155, 1216
SfaNI GCATC 4 cut(s) 370, 681, 1060, 1117
SfcI CTRYAG 1 cut(s) 416
SfoI GGCGCC 1 cut(s) 1202
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 2 cut(s) 767, 889
SmlI CTYRAG 2 cut(s) 181, 341
SmoI CTYRAG 2 cut(s) 181, 341
SpeI ACTAGT 1 cut(s) 466
Sse9I AATT 7 cut(s) 94, 176, 228, 386, 814, 927, 1219
SseBI AGGCCT 2 cut(s) 731, 781
SsiI CCGC 5 cut(s) 217, 220, 593, 1073, 1076
SspDI GGCGCC 1 cut(s) 1200
SspI AATATT 1 cut(s) 211
SspMI CTAG 3 cut(s) 27, 467, 792
StuI AGGCCT 2 cut(s) 731, 781
StyD4I CCNGG 3 cut(s) 456, 478, 1057
StyI CCWWGG 2 cut(s) 60, 1014
TaaI ACNGT 4 cut(s) 156, 901, 953, 1086
TaqI TCGA 2 cut(s) 101, 545
TasI AATT 7 cut(s) 94, 176, 228, 386, 814, 927, 1219
TatI WGTACW 2 cut(s) 988, 1081
TauI GCSGC 2 cut(s) 222, 1076
TfiI GAWTC 5 cut(s) 250, 322, 430, 589, 968
Tru1I TTAA 2 cut(s) 120, 182
Tru9I TTAA 2 cut(s) 120, 182
TscAI CASTG 2 cut(s) 161, 904
TseI GCWGC 2 cut(s) 115, 502
TspDTI ATGAA 4 cut(s) 221, 785, 836, 950
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 161, 904
Vha464I CTTAAG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 3 cut(s) 176, 386, 814
XmnI GAANNNNTTC 2 cut(s) 69, 666
XspI CTAG 3 cut(s) 27, 467, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.