Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002221

Physical Location & Seq

Genomic Coordinates Forward (+)

36595397 .. 36597885

Gene Structure 2489 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1274400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGAGGAAGTTCAAGGTGGCACGAGTTCGCTGCCTCCTTTCCTTTCCAAGACATATGAGATGGTAGATGATGCTTTAACAGATTCAATTGTGTCATGGAGTGCCAGTAATAAGAGCTTCATTGTTTGGAATCCGCCGGAGTTCGCTAGAGATTTGCTGCCAAAGTTCTTCAAGCACAACAACTTCTCTAGCTTCATCAGGCAGCTCAATACCTACGGTTTTAGAAAAATTGATCCGGAGCAATGGGAATTCACCAATGACGATTTTATTAGAGGTCAGCCACACATTATGAAGAAGATCCGTAGGCGAAAGCCGGTGCACAGCCACTCTTTGCAGAATCTTCAAGTCCAAGGGCAAGGGACTTCATTGTCCGAAGCTGAACGGCAGAGTATGAAGGATGAAATCGAGAGGCTTAAACATGAGAAAGAACGCCTTGGTGTGGAGGTACAGAGGCATGAGCAGGAGCGGCATGAATTGGAGTTGCAGATGCAGTTCTTGATGGACCGTTTGCAACACATGGAAAAGCAGCAGCAAAGCATGGCGGCTTTCGTGGCTAGGGTTTTGCACAAACCGGGGATTGCCTCCAATCCTGTTCCGCAGTTAGAAGTCCGTGAGCGAAAGAGAAGGTTGCCAAGAATCAGTTGGTCCTTCGATGATGCTAACAGTGGATATAATCAGATGGTTACTTCACAAGCTGTAATTAGAGAAAATGGGGGTACGGAAAAGTTGGAGCAGTTGGAGTCTTTCCTAACATTTTGGGAAGATACCATTCATGATGTTGGTCACAATAACGTCCAGGTTGGTTCAAACCGAGAGGCGGATGAATCTACAAGTGGTAACGAAAGCACAGCTCTGTCTTCCATACAACTGAATGCTGACATTCGGTCAAAATCCCCTGGAATTGACATGAATGCTGAACCCCCCGCTTCTGTTGCTCCCAAGCCTGCTGCATCACAATCCTCGAAAGAACAAACAGCTGGAATTGCTGCTTCTGCACCCACCACCGCCTTGACACAACAAACGTCTAAAGAAAAACCAGCTGGAACCACTGCTTCTGCACCCACCGGGGTCAATGACATATTTTGGGAACAGTTCTTAACAGAGAATCCTGGTACATCGGAAGCACAGGAAGTTCTGATTGAAAGAAAGGATTCCGATGGTAAAACGAATGACAGCAAACCTGGTGATCATGGTATGTTGTGGTGGAATATGAGGAATGTAAATAACCTTACAGAACAGATGGGGCATCTTACTCCAGTAGAGAAAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

424

Amino Acids

47.79

Weight (kDa)

5.7

Isoelectric Point (pI)

47.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	14 - 103	2.6e-29	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18880 AT5G45710 AT5G45710 AT5G45710 AT5G45710
fragaria_vesca	FvH4_3g07170 FvH4_3g07170 FvH4_6g27900
malus_domestica	MD00G1007800.v1.1 MD09G1233700.v1.1 MD10G1274400.v1.1 MD17G1230100.v1.1
prunus_persica	Prupe.3G108700_v2.0.a1 Prupe.3G108700_v2.0.a1 Prupe.4G068100_v2.0.a1 Prupe.4G068100_v2.0.a1 Prupe.4G068100_v2.0.a1 Prupe.4G068100_v2.0.a1 Prupe.4G068100_v2.0.a1
pyrus_communis	pycom05g27300 pycom09g15200 pycom10g22900 pycom17g23300
rosa_chinensis	RchiOBHm_Chr2g0131371 RchiOBHm_Chr5g0011961
rosa_laevigata	RLG00000019181 RLG00000031873
rosa_multiflora	Rmu_sc0001475.1_g000001 Rmu_ssc0000009.1_g000034
rosa_roxburghii	Rroxscaffold_1G00064630 Rroxscaffold_2G00113040
rosa_rugosa	Rorug02G0296200 Rorug04G0460200
rosa_samantha	Rh2AG349500 Rh2BG356400 Rh2CG335300 Rh2DG374300 Rh5AG091800 Rh5BG087700 Rh5CG101500 Rh5DG088300
rosa_wichuraiana	Rw2G028360 Rw5G008020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	367
AccBSI	CCGCTC	1 cut(s)	466
AccIII	TCCGGA	1 cut(s)	235
AciI	CCGC	7 cut(s)	134, 466, 542, 596, 818, 924, 1005
AclWI	GGATC	2 cut(s)	227, 292
AcsI	RAATTY	1 cut(s)	248
AfaI	GTAC	3 cut(s)	447, 718, 1114
AfiI	CCNNNNNNNGG	2 cut(s)	439, 817
AgsI	TTSAA	6 cut(s)	14, 87, 172, 344, 807, 1142
AhdI	GACNNNNNGTC	1 cut(s)	883
AjnI	CCWGG	4 cut(s)	795, 895, 1108, 1180
AluBI	AGCT	8 cut(s)	117, 192, 205, 377, 695, 851, 977, 1040
AluI	AGCT	8 cut(s)	117, 192, 205, 377, 695, 851, 977, 1040
Alw21I	GWGCWC	1 cut(s)	321
Alw44I	GTGCAC	1 cut(s)	317
AlwI	GGATC	2 cut(s)	227, 292
Aor13HI	TCCGGA	1 cut(s)	235
ApaLI	GTGCAC	1 cut(s)	317
ApeKI	GCWGC	7 cut(s)	31, 157, 202, 526, 529, 947, 986
ApoI	RAATTY	1 cut(s)	248
Asp700I	GAANNNNTTC	1 cut(s)	1150
AspS9I	GGNCC	2 cut(s)	502, 645
AsuC2I	CCSGG	2 cut(s)	573, 1066
AsuHPI	GGTGA	2 cut(s)	244, 1196
AvaII	GGWCC	2 cut(s)	502, 645
BaeGI	GKGCMC	1 cut(s)	321
BauI	CACGAG	1 cut(s)	22
BbsI	GAAGAC	1 cut(s)	849
Bbv12I	GWGCWC	1 cut(s)	321
BbvI	GCAGC	7 cut(s)	18, 144, 214, 538, 541, 934, 973
BccI	CCATC	5 cut(s)	55, 493, 673, 1151, 1234
BceAI	ACGGC	1 cut(s)	398
BcgI	CGANNNNNNTGC	2 cut(s)	13, 47
BciT130I	CCWGG	4 cut(s)	797, 897, 1110, 1182
BclI	TGATCA	1 cut(s)	1186
BcnI	CCSGG	2 cut(s)	573, 1066
BfaI	CTAG	3 cut(s)	147, 189, 555
BisI	GCNGC	9 cut(s)	32, 158, 203, 467, 527, 530, 543, 948, 987
BlsI	GCNGC	9 cut(s)	33, 159, 204, 468, 528, 531, 544, 949, 988
Bme1390I	CCNGG	6 cut(s)	573, 797, 897, 1066, 1110, 1182
Bme18I	GGWCC	2 cut(s)	502, 645
BmeRI	GACNNNNNGTC	1 cut(s)	883
BmgT120I	GGNCC	2 cut(s)	502, 645
BmiI	GGNNCC	1 cut(s)	1045
BmrFI	CCNGG	6 cut(s)	573, 797, 897, 1066, 1110, 1182
BmsI	GCATC	5 cut(s)	61, 477, 646, 959, 1255
BpiI	GAAGAC	1 cut(s)	849
BpmI	CTGGAG	1 cut(s)	1239
BpuMI	CCSGG	2 cut(s)	573, 1066
BsaJI	CCNNGG	5 cut(s)	349, 433, 572, 895, 1065
BsaWI	WCCGGW	1 cut(s)	235
BsaXI	ACNNNNNCTCC	2 cut(s)	91, 121
Bsc4I	CCNNNNNNNGG	2 cut(s)	439, 817
Bse118I	RCCGGY	1 cut(s)	313
Bse1I	ACTGG	2 cut(s)	105, 1256
Bse3DI	GCAATG	1 cut(s)	248
BseAI	TCCGGA	1 cut(s)	235
BseBI	CCWGG	4 cut(s)	797, 897, 1110, 1182
BseDI	CCNNGG	5 cut(s)	349, 433, 572, 895, 1065
BseGI	GGATG	2 cut(s)	403, 826
BseLI	CCNNNNNNNGG	2 cut(s)	439, 817
BseMI	GCAATG	1 cut(s)	248
BseNI	ACTGG	2 cut(s)	105, 1256
BseSI	GKGCMC	1 cut(s)	321
BseXI	GCAGC	7 cut(s)	18, 144, 214, 538, 541, 934, 973
BsgI	GTGCAG	2 cut(s)	978, 1041
BsiHKAI	GWGCWC	1 cut(s)	321
BsiSI	CCGG	5 cut(s)	137, 236, 314, 572, 1065
BslFI	GGGAC	1 cut(s)	373
BslI	CCNNNNNNNGG	2 cut(s)	439, 817
BsmFI	GGGAC	1 cut(s)	373
BsmI	GAATGC	2 cut(s)	877, 916
Bsp1286I	GDGCHC	1 cut(s)	321
Bsp13I	TCCGGA	1 cut(s)	235
Bsp143I	GATC	3 cut(s)	232, 297, 1186
BspACI	CCGC	7 cut(s)	134, 466, 542, 596, 818, 924, 1005
BspEI	TCCGGA	1 cut(s)	235
BspHI	TCATGA	1 cut(s)	772
BspLI	GGNNCC	1 cut(s)	1045
BspPI	GGATC	2 cut(s)	227, 292
BsrBI	CCGCTC	1 cut(s)	466
BsrDI	GCAATG	1 cut(s)	248
BsrFI	RCCGGY	1 cut(s)	313
BsrI	ACTGG	2 cut(s)	105, 1256
BssAI	RCCGGY	1 cut(s)	313
BssECI	CCNNGG	5 cut(s)	349, 433, 572, 895, 1065
BssMI	GATC	3 cut(s)	232, 297, 1186
BssSI	CACGAG	1 cut(s)	22
BssT1I	CCWWGG	2 cut(s)	349, 433
Bst2BI	CACGAG	1 cut(s)	22
Bst2UI	CCWGG	4 cut(s)	797, 897, 1110, 1182
Bst4CI	ACNGT	4 cut(s)	218, 506, 665, 1092
BstC8I	GCNNGC	1 cut(s)	945
BstF5I	GGATG	2 cut(s)	403, 826
BstKTI	GATC	3 cut(s)	235, 300, 1189
BstMBI	GATC	3 cut(s)	232, 297, 1186
BstMWI	GCNNNNNNNGC	5 cut(s)	466, 551, 932, 983, 992
BstNI	CCWGG	4 cut(s)	797, 897, 1110, 1182
BstSCI	CCNGG	6 cut(s)	571, 795, 895, 1064, 1108, 1180
BstSLI	GKGCMC	1 cut(s)	321
BstV1I	GCAGC	7 cut(s)	18, 144, 214, 538, 541, 934, 973
BstV2I	GAAGAC	1 cut(s)	849
BstX2I	RGATCY	1 cut(s)	297
BstYI	RGATCY	1 cut(s)	297
BtsCI	GGATG	2 cut(s)	403, 826
BtsI	GCAGTG	1 cut(s)	1047
BtsIMutI	CAGTG	2 cut(s)	670, 1047
Cac8I	GCNNGC	1 cut(s)	945
CciI	TCATGA	1 cut(s)	772
Cfr10I	RCCGGY	1 cut(s)	313
Cfr13I	GGNCC	2 cut(s)	502, 645
CsiI	ACCWGGT	1 cut(s)	1180
Csp6I	GTAC	3 cut(s)	446, 717, 1113
CviAII	CATG	9 cut(s)	96, 419, 455, 470, 517, 538, 773, 907, 1190
CviQI	GTAC	3 cut(s)	446, 717, 1113
DpnI	GATC	3 cut(s)	234, 299, 1188
DpnII	GATC	3 cut(s)	232, 297, 1186
DrdI	GACNNNNNNGTC	1 cut(s)	367
DriI	GACNNNNNGTC	1 cut(s)	883
DseDI	GACNNNNNNGTC	1 cut(s)	367
Eam1105I	GACNNNNNGTC	1 cut(s)	883
EciI	GGCGGA	2 cut(s)	123, 833
Eco130I	CCWWGG	2 cut(s)	349, 433
Eco47I	GGWCC	2 cut(s)	502, 645
EcoRI	GAATTC	1 cut(s)	248
EcoRII	CCWGG	4 cut(s)	795, 895, 1108, 1180
EcoT14I	CCWWGG	2 cut(s)	349, 433
ErhI	CCWWGG	2 cut(s)	349, 433
FaeI	CATG	9 cut(s)	99, 422, 458, 473, 520, 541, 776, 910, 1193
FalI	AAGNNNNNCTT	2 cut(s)	417, 449
FaqI	GGGAC	1 cut(s)	373
FatI	CATG	9 cut(s)	95, 418, 454, 469, 516, 537, 772, 906, 1189
FauI	CCCGC	1 cut(s)	931
FauNDI	CATATG	1 cut(s)	55
FbaI	TGATCA	1 cut(s)	1186
Fnu4HI	GCNGC	9 cut(s)	32, 158, 203, 467, 527, 530, 543, 948, 987
FokI	GGATG	2 cut(s)	410, 833
Fsp4HI	GCNGC	9 cut(s)	32, 158, 203, 467, 527, 530, 543, 948, 987
FspBI	CTAG	3 cut(s)	147, 189, 555
GluI	GCNGC	9 cut(s)	32, 158, 203, 467, 527, 530, 543, 948, 987
GsuI	CTGGAG	1 cut(s)	1239
HapII	CCGG	5 cut(s)	137, 236, 314, 572, 1065
Hin1II	CATG	9 cut(s)	99, 422, 458, 473, 520, 541, 776, 910, 1193
HinfI	GANTC	8 cut(s)	83, 130, 337, 636, 740, 824, 1105, 1151
HpaII	CCGG	5 cut(s)	137, 236, 314, 572, 1065
HphI	GGTGA	2 cut(s)	244, 1196
Hpy166II	GTNNAC	1 cut(s)	319
Hpy188I	TCNGA	5 cut(s)	373, 678, 1120, 1137, 1156
Hpy188III	TCNNGA	4 cut(s)	236, 406, 496, 773
Hpy8I	GTNNAC	1 cut(s)	319
HpyAV	CCTTC	3 cut(s)	388, 618, 658
HpyCH4III	ACNGT	4 cut(s)	218, 506, 665, 1092
HpyCH4IV	ACGT	2 cut(s)	792, 1022
HpyCH4V	TGCA	9 cut(s)	319, 334, 484, 490, 511, 565, 950, 995, 1058
HpyF10VI	GCNNNNNNNGC	5 cut(s)	466, 551, 932, 983, 992
HpySE526I	ACGT	2 cut(s)	792, 1022
Hsp92II	CATG	9 cut(s)	99, 422, 458, 473, 520, 541, 776, 910, 1193
Kpn2I	TCCGGA	1 cut(s)	235
Ksp22I	TGATCA	1 cut(s)	1186
Kzo9I	GATC	3 cut(s)	232, 297, 1186
LmnI	GCTCC	4 cut(s)	238, 463, 730, 940
Lsp1109I	GCAGC	7 cut(s)	18, 144, 214, 538, 541, 934, 973
LweI	GCATC	5 cut(s)	61, 477, 646, 959, 1255
MabI	ACCWGGT	1 cut(s)	1180
MaeI	CTAG	3 cut(s)	147, 189, 555
MaeII	ACGT	2 cut(s)	792, 1022
MaeIII	GTNAC	3 cut(s)	682, 782, 836
MalI	GATC	3 cut(s)	234, 299, 1188
MbiI	CCGCTC	1 cut(s)	466
MboI	GATC	3 cut(s)	232, 297, 1186
MboII	GAAGA	6 cut(s)	160, 304, 307, 332, 773, 849
MfeI	CAATTG	1 cut(s)	87
MflI	RGATCY	1 cut(s)	297
MhlI	GDGCHC	1 cut(s)	321
MluCI	AATT	7 cut(s)	87, 228, 248, 473, 699, 900, 981
MlyI	GAGTC	1 cut(s)	749
MmeI	TCCRAC	2 cut(s)	708, 717
MnlI	CCTC	9 cut(s)	45, 266, 402, 436, 444, 592, 808, 970, 1206
MroI	TCCGGA	1 cut(s)	235
MroXI	GAANNNNTTC	1 cut(s)	1150
MseI	TTAA	3 cut(s)	77, 414, 1097
MspA1I	CMGCKG	2 cut(s)	977, 1040
MspI	CCGG	5 cut(s)	137, 236, 314, 572, 1065
MspR9I	CCNGG	6 cut(s)	573, 797, 897, 1066, 1110, 1182
MunI	CAATTG	1 cut(s)	87
Mva1269I	GAATGC	2 cut(s)	877, 916
MvaI	CCWGG	4 cut(s)	797, 897, 1110, 1182
MwoI	GCNNNNNNNGC	5 cut(s)	466, 551, 932, 983, 992
NciI	CCSGG	2 cut(s)	573, 1066
NdeI	CATATG	1 cut(s)	55
NdeII	GATC	3 cut(s)	232, 297, 1186
NlaIII	CATG	9 cut(s)	99, 422, 458, 473, 520, 541, 776, 910, 1193
NlaIV	GGNNCC	1 cut(s)	1045
NmuCI	GTSAC	1 cut(s)	782
PagI	TCATGA	1 cut(s)	772
PctI	GAATGC	2 cut(s)	877, 916
PdmI	GAANNNNTTC	1 cut(s)	1150
PfeI	GAWTC	7 cut(s)	83, 130, 337, 636, 824, 1105, 1151
PkrI	GCNGC	9 cut(s)	33, 159, 204, 468, 528, 531, 544, 949, 988
PleI	GAGTC	1 cut(s)	748
PpsI	GAGTC	1 cut(s)	748
Psp6I	CCWGG	4 cut(s)	795, 895, 1108, 1180
PspGI	CCWGG	4 cut(s)	795, 895, 1108, 1180
PspN4I	GGNNCC	1 cut(s)	1045
PspPI	GGNCC	2 cut(s)	502, 645
PsuI	RGATCY	1 cut(s)	297
PvuII	CAGCTG	2 cut(s)	977, 1040
RsaI	GTAC	3 cut(s)	447, 718, 1114
RsaNI	GTAC	3 cut(s)	446, 717, 1113
SaqAI	TTAA	3 cut(s)	77, 414, 1097
SatI	GCNGC	9 cut(s)	32, 158, 203, 467, 527, 530, 543, 948, 987
Sau3AI	GATC	3 cut(s)	232, 297, 1186
Sau96I	GGNCC	2 cut(s)	502, 645
SchI	GAGTC	1 cut(s)	749
ScrFI	CCNGG	6 cut(s)	573, 797, 897, 1066, 1110, 1182
SduI	GDGCHC	1 cut(s)	321
SexAI	ACCWGGT	1 cut(s)	1180
SfaNI	GCATC	5 cut(s)	61, 477, 646, 959, 1255
SinI	GGWCC	2 cut(s)	502, 645
Sse9I	AATT	7 cut(s)	87, 228, 248, 473, 699, 900, 981
SsiI	CCGC	7 cut(s)	134, 466, 542, 596, 818, 924, 1005
SspMI	CTAG	3 cut(s)	147, 189, 555
StyD4I	CCNGG	6 cut(s)	571, 795, 895, 1064, 1108, 1180
StyI	CCWWGG	2 cut(s)	349, 433
TaaI	ACNGT	4 cut(s)	218, 506, 665, 1092
TaiI	ACGT	2 cut(s)	795, 1025
TaqI	TCGA	3 cut(s)	405, 651, 962
TaqII	GACCGA	1 cut(s)	873
TasI	AATT	7 cut(s)	87, 228, 248, 473, 699, 900, 981
TauI	GCSGC	2 cut(s)	469, 545
TfiI	GAWTC	7 cut(s)	83, 130, 337, 636, 824, 1105, 1151
Tru1I	TTAA	3 cut(s)	77, 414, 1097
Tru9I	TTAA	3 cut(s)	77, 414, 1097
TscAI	CASTG	2 cut(s)	670, 1054
TseFI	GTSAC	1 cut(s)	782
TseI	GCWGC	7 cut(s)	31, 157, 202, 526, 529, 947, 986
Tsp45I	GTSAC	1 cut(s)	782
TspGWI	ACGGA	3 cut(s)	290, 599, 734
TspRI	CASTG	2 cut(s)	670, 1054
VneI	GTGCAC	1 cut(s)	317
VpaK11BI	GGWCC	2 cut(s)	502, 645
XapI	RAATTY	1 cut(s)	248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	639
XmnI	GAANNNNTTC	1 cut(s)	1150
XspI	CTAG	3 cut(s)	147, 189, 555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.