Rh2AG349500

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002221
Physical Location & Seq
Reverse (-)
51341406 .. 51345447
4042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG349500.1

Sequence Viewer

Length: 1317 bp
ATGGATGGGTCTCAAGGTGGTTCGAATGCACCAGCTCCTTTTCTTTCGAAAACATATGACTTGGTGGATGATCCTTCAACCAACCATGTGGTGTCTTGGAGTGAAGCTGGTTGTAGTTTTGTAGTTTGGGATCCCACAGAGTTTGCCAAGGATCTGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTTGTCAGGCAGCTTAATACATATGGGTTTAGGAAGGTTGATCCTGAGCAGTGGGAGTTTGCAAATGAGGAATTCGTAAGAGGAGGAAGGCATCTACTGAAGAATATTCACCGCCGCAAGCCAATTCATAGTCACTCCATGCAAAATCATGAGTATTCTTCAATTCCTTTAAGCGAGAAAGAAAGAGAAGAATATGAAAATAAAATCAATAGACTGAATCAGGAGAAGAGCTTGCTCGAGTCAGATCTACAAAGACATCAAAAGGAGAATCAGGCATTTGAATTTCAAATGCAATTGTTAACTGAACAATTGCAAAAGATGGAACTTCGGCAGAGGCAATATACGACCTTCCTAGCTCAAGTATTGAAGAAACCAGGGTTTGCCTCTTTACTTATGCAACAATCGGAAAATCATAACAAAAAGAGAAGACTGTTGAAACCTAACCTCTTTCCTGATGACTTCAGTATGGAAGGTTTGAATTTGAATTCACAGAAAGAGAACCTGGACTCAGTTTCAACTTCCACATCAAAGTCAGACCGGCTTGAGAAAATGGAGTCTTCCTTAAACTTTTGGGAAGATTTTCTACGTGGTATTGAGGAAGCTATACCTGAAGAAGTAAATGATATTGGCCCTTTGTCTGAGGCCTCTCCCATCATTGTTTCAGAAATGCAAGACCTTGGCATGAATAGCAGGCCGTGCTCACCTATATCGAATTTATCTTCGCCGACTTCAATGAATGTTCATTCATCCCCAGAGGCTGGGTCTGCAAATTTTTTCGACATCCTTGCTATATCATCAATGTGTCAAACTGTTGATTTCAGGACTAAATCTTCAGGACTTGATATGAACTCTAAGCCTGATAGTGCACCTGCAGTTGAGGCTCCAAAAGAAAGGGTAGTAGAAATGACAACCGCTGAGCCTGCTGCAGCAAATGATGTCTTTTGGGAGCAATGTCTAACAGAGACCCCCGGTTTAGAAGATGCACAGGAAGCACAATCGGAAAGAAGGGATGGCGATGGCGGAGCGGGTGATACCAATGCAGCTGCTCAGAAAAAGCTTTGGTGGAACACAGATAGTGTAGATAACTTTACAAACCAAATAGGGCGCCTCACTCCAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

438

Amino Acids

49.56

Weight (kDa)

4.98

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 14 - 103 1.7e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1069
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 1296
AccB7I CCANNNNNTGG 1 cut(s) 950
AccBSI CCGCTC 1 cut(s) 1217
AciI CCGC 5 cut(s) 304, 307, 1104, 1212, 1217
AclWI GGATC 5 cut(s) 65, 125, 138, 159, 227
AcsI RAATTY 6 cut(s) 263, 473, 670, 676, 904, 961
AcuI CTGAAG 4 cut(s) 311, 637, 822, 1008
AcyI GRCGYC 1 cut(s) 1297
AfiI CCNNNNNNNGG 1 cut(s) 950
AjnI CCWGG 2 cut(s) 565, 693
AluBI AGCT 9 cut(s) 35, 107, 205, 423, 548, 794, 1235, 1249, 1310
AluI AGCT 9 cut(s) 35, 107, 205, 423, 548, 794, 1235, 1249, 1310
Alw21I GWGCWC 2 cut(s) 893, 1060
Alw26I GTCTC 2 cut(s) 15, 1148
Alw44I GTGCAC 1 cut(s) 1056
AlwI GGATC 5 cut(s) 65, 125, 138, 159, 227
AlwNI CAGNNNCTG 1 cut(s) 950
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 3 cut(s) 820, 834, 884
ApaLI GTGCAC 1 cut(s) 1056
ApeKI GCWGC 5 cut(s) 202, 1115, 1118, 1232, 1235
ApoI RAATTY 6 cut(s) 263, 473, 670, 676, 904, 961
Asp700I GAANNNNTTC 1 cut(s) 771
AspLEI GCGC 1 cut(s) 1299
AspS9I GGNCC 1 cut(s) 821
AsuC2I CCSGG 1 cut(s) 1161
AsuHPI GGTGA 3 cut(s) 293, 885, 1232
AsuII TTCGAA 2 cut(s) 23, 47
AvaI CYCGRG 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 1060
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 1296
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BbsI GAAGAC 2 cut(s) 625, 741
Bbv12I GWGCWC 2 cut(s) 893, 1060
BbvI GCAGC 5 cut(s) 214, 1102, 1130, 1222, 1244
BccI CCATC 4 cut(s) 505, 851, 1196, 1202
BceAI ACGGC 1 cut(s) 871
BcgI CGANNNNNNTGC 2 cut(s) 959, 993
BciT130I CCWGG 2 cut(s) 567, 695
BcnI CCSGG 1 cut(s) 1161
BcoDI GTCTC 2 cut(s) 15, 1148
BfaI CTAG 1 cut(s) 545
BfmI CTRYAG 2 cut(s) 1062, 1116
BfoI RGCGCY 1 cut(s) 1300
BfuAI ACCTGC 1 cut(s) 1069
BglII AGATCT 1 cut(s) 436
BisI GCNGC 6 cut(s) 203, 307, 1116, 1119, 1233, 1236
BlpI GCTNAGC 1 cut(s) 1107
BlsI GCNGC 6 cut(s) 204, 308, 1117, 1120, 1234, 1237
Bme1390I CCNGG 3 cut(s) 567, 695, 1161
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 132, 1074, 1298
BmrFI CCNGG 3 cut(s) 567, 695, 1161
BmsI GCATC 2 cut(s) 292, 1162
BpiI GAAGAC 2 cut(s) 625, 741
BpmI CTGGAG 1 cut(s) 1290
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 1107
Bpu14I TTCGAA 2 cut(s) 23, 47
BpuEI CTTGAG 2 cut(s) 534, 755
BpuMI CCSGG 1 cut(s) 1161
BsaAI YACGTR 1 cut(s) 779
BsaHI GRCGYC 1 cut(s) 1297
BsaI GGTCTC 2 cut(s) 15, 1148
BsaJI CCNNGG 4 cut(s) 147, 566, 868, 1159
Bsc4I CCNNNNNNNGG 1 cut(s) 950
Bse118I RCCGGY 1 cut(s) 729
Bse3DI GCAATG 1 cut(s) 1148
BseBI CCWGG 2 cut(s) 567, 695
BseDI CCNNGG 4 cut(s) 147, 566, 868, 1159
BseGI GGATG 5 cut(s) 10, 73, 938, 972, 1207
BseLI CCNNNNNNNGG 1 cut(s) 950
BseMI GCAATG 1 cut(s) 1148
BseMII CTCAG 5 cut(s) 228, 714, 822, 1098, 1253
BseRI GAGGAG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 5 cut(s) 214, 1102, 1130, 1222, 1244
BseYI CCCAGC 1 cut(s) 950
BshFI GGCC 3 cut(s) 822, 836, 886
BshNI GGYRCC 1 cut(s) 1296
BsiHKAI GWGCWC 2 cut(s) 893, 1060
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 2 cut(s) 730, 1161
BslI CCNNNNNNNGG 1 cut(s) 950
BsmAI GTCTC 2 cut(s) 15, 1148
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 3 cut(s) 822, 836, 886
Bso31I GGTCTC 2 cut(s) 15, 1148
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 2 cut(s) 23, 47
Bsp1286I GDGCHC 2 cut(s) 893, 1060
Bsp143I GATC 5 cut(s) 70, 130, 151, 232, 436
Bsp1720I GCTNAGC 1 cut(s) 1107
BspACI CCGC 5 cut(s) 304, 307, 1104, 1212, 1217
BspANI GGCC 3 cut(s) 822, 836, 886
BspCNI CTCAG 5 cut(s) 229, 713, 823, 1099, 1252
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 132, 1074, 1298
BspMAI CTGCAG 2 cut(s) 1066, 1120
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 5 cut(s) 65, 125, 138, 159, 227
BspQI GCTCTTC 1 cut(s) 413
BspT104I TTCGAA 2 cut(s) 23, 47
BspT107I GGYRCC 1 cut(s) 1296
BspTNI GGTCTC 2 cut(s) 15, 1148
BsrBI CCGCTC 1 cut(s) 1217
BsrDI GCAATG 1 cut(s) 1148
BsrFI RCCGGY 1 cut(s) 729
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 4 cut(s) 147, 566, 868, 1159
BssMI GATC 5 cut(s) 70, 130, 151, 232, 436
BssNI GRCGYC 1 cut(s) 1297
BssT1I CCWWGG 2 cut(s) 147, 868
Bst2UI CCWGG 2 cut(s) 567, 695
Bst4CI ACNGT 2 cut(s) 624, 1003
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 1297
BstAPI GCANNNNNTGC 1 cut(s) 888
BstBAI YACGTR 1 cut(s) 779
BstBI TTCGAA 2 cut(s) 23, 47
BstC8I GCNNGC 4 cut(s) 311, 425, 884, 1113
BstDEI CTNAG 6 cut(s) 237, 700, 831, 1044, 1107, 1239
BstF5I GGATG 5 cut(s) 10, 73, 938, 972, 1207
BstH2I RGCGCY 1 cut(s) 1300
BstHHI GCGC 1 cut(s) 1299
BstKTI GATC 5 cut(s) 73, 133, 154, 235, 439
BstMAI GTCTC 2 cut(s) 15, 1148
BstMBI GATC 5 cut(s) 70, 130, 151, 232, 436
BstMWI GCNNNNNNNGC 6 cut(s) 879, 888, 956, 1070, 1112, 1181
BstNI CCWGG 2 cut(s) 567, 695
BstSCI CCNGG 3 cut(s) 565, 693, 1159
BstSFI CTRYAG 2 cut(s) 1062, 1116
BstSLI GKGCMC 1 cut(s) 1060
BstV1I GCAGC 5 cut(s) 214, 1102, 1130, 1222, 1244
BstV2I GAAGAC 2 cut(s) 625, 741
BstX2I RGATCY 3 cut(s) 130, 151, 436
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 3 cut(s) 130, 151, 436
BsuRI GGCC 3 cut(s) 822, 836, 886
BtgZI GCGATG 1 cut(s) 1221
BtsCI GGATG 5 cut(s) 10, 73, 938, 972, 1207
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 4 cut(s) 311, 425, 884, 1113
CaiI CAGNNNCTG 1 cut(s) 950
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 1299
Cfr10I RCCGGY 1 cut(s) 729
Cfr13I GGNCC 1 cut(s) 821
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 5 cut(s) 86, 163, 331, 341, 874
DdeI CTNAG 6 cut(s) 237, 700, 831, 1044, 1107, 1239
DinI GGCGCC 1 cut(s) 1298
DpnI GATC 5 cut(s) 72, 132, 153, 234, 438
DpnII GATC 5 cut(s) 70, 130, 151, 232, 436
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 1227
Eco130I CCWWGG 2 cut(s) 147, 868
Eco147I AGGCCT 1 cut(s) 836
Eco31I GGTCTC 2 cut(s) 15, 1148
Eco57I CTGAAG 4 cut(s) 311, 637, 822, 1008
Eco88I CYCGRG 1 cut(s) 428
EcoRI GAATTC 2 cut(s) 263, 676
EcoRII CCWGG 2 cut(s) 565, 693
EcoT14I CCWWGG 2 cut(s) 147, 868
EgeI GGCGCC 1 cut(s) 1298
EheI GGCGCC 1 cut(s) 1298
ErhI CCWWGG 2 cut(s) 147, 868
FaeI CATG 5 cut(s) 89, 166, 334, 344, 877
FatI CATG 5 cut(s) 85, 162, 330, 340, 873
FauI CCCGC 1 cut(s) 1210
FauNDI CATATG 2 cut(s) 55, 214
Fnu4HI GCNGC 6 cut(s) 203, 307, 1116, 1119, 1233, 1236
FokI GGATG 5 cut(s) 17, 80, 925, 959, 1214
Fsp4HI GCNGC 6 cut(s) 203, 307, 1116, 1119, 1233, 1236
FspBI CTAG 1 cut(s) 545
GlaI GCGC 1 cut(s) 1298
GluI GCNGC 6 cut(s) 203, 307, 1116, 1119, 1233, 1236
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 1 cut(s) 1290
HaeII RGCGCY 1 cut(s) 1300
HaeIII GGCC 3 cut(s) 822, 836, 886
HapII CCGG 2 cut(s) 730, 1161
HhaI GCGC 1 cut(s) 1299
Hin1I GRCGYC 1 cut(s) 1297
Hin1II CATG 5 cut(s) 89, 166, 334, 344, 877
Hin6I GCGC 1 cut(s) 1297
HinP1I GCGC 1 cut(s) 1297
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HindIII AAGCTT 1 cut(s) 1247
HinfI GANTC 5 cut(s) 409, 431, 460, 698, 746
HpaI GTTAAC 1 cut(s) 492
HpaII CCGG 2 cut(s) 730, 1161
HphI GGTGA 3 cut(s) 293, 885, 1232
Hpy166II GTNNAC 2 cut(s) 492, 1058
Hpy188I TCNGA 7 cut(s) 436, 598, 727, 832, 856, 1192, 1242
Hpy188III TCNNGA 6 cut(s) 236, 341, 413, 644, 1012, 1026
Hpy8I GTNNAC 2 cut(s) 492, 1058
HpyAV CCTTC 6 cut(s) 84, 220, 273, 550, 656, 1191
HpyCH4III ACNGT 2 cut(s) 624, 1003
HpyCH4IV ACGT 1 cut(s) 778
HpyF10VI GCNNNNNNNGC 6 cut(s) 879, 888, 956, 1070, 1112, 1181
HpyF3I CTNAG 6 cut(s) 237, 700, 831, 1044, 1107, 1239
HpySE526I ACGT 1 cut(s) 778
Hsp92I GRCGYC 1 cut(s) 1297
Hsp92II CATG 5 cut(s) 89, 166, 334, 344, 877
HspAI GCGC 1 cut(s) 1297
KasI GGCGCC 1 cut(s) 1296
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 5 cut(s) 70, 130, 151, 232, 436
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 4 cut(s) 40, 1078, 1138, 1214
Lsp1109I GCAGC 5 cut(s) 214, 1102, 1130, 1222, 1244
LweI GCATC 2 cut(s) 292, 1162
MaeI CTAG 1 cut(s) 545
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 1 cut(s) 323
MalI GATC 5 cut(s) 72, 132, 153, 234, 438
MbiI CCGCTC 1 cut(s) 1217
MboI GATC 5 cut(s) 70, 130, 151, 232, 436
MfeI CAATTG 2 cut(s) 485, 500
MflI RGATCY 3 cut(s) 130, 151, 436
MhlI GDGCHC 2 cut(s) 893, 1060
Mly113I GGCGCC 1 cut(s) 1297
MlyI GAGTC 3 cut(s) 440, 692, 755
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 4 cut(s) 207, 362, 491, 755
MslI CAYNNNNRTG 1 cut(s) 991
MspA1I CMGCKG 2 cut(s) 1106, 1235
MspI CCGG 2 cut(s) 730, 1161
MspR9I CCNGG 3 cut(s) 567, 695, 1161
MunI CAATTG 2 cut(s) 485, 500
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 567, 695
MwoI GCNNNNNNNGC 6 cut(s) 879, 888, 956, 1070, 1112, 1181
NarI GGCGCC 1 cut(s) 1297
NciI CCSGG 1 cut(s) 1161
NdeI CATATG 2 cut(s) 55, 214
NdeII GATC 5 cut(s) 70, 130, 151, 232, 436
NlaIII CATG 5 cut(s) 89, 166, 334, 344, 877
NlaIV GGNNCC 3 cut(s) 132, 1074, 1298
NmuCI GTSAC 1 cut(s) 323
NspV TTCGAA 2 cut(s) 23, 47
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 1069
PceI AGGCCT 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 413
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 2 cut(s) 409, 460
PflMI CCANNNNNTGG 1 cut(s) 950
PkrI GCNGC 6 cut(s) 204, 308, 1117, 1120, 1234, 1237
PleI GAGTC 3 cut(s) 439, 692, 754
PluTI GGCGCC 1 cut(s) 1300
PpsI GAGTC 3 cut(s) 439, 692, 754
Ppu21I YACGTR 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 565, 693
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 2 cut(s) 565, 693
PspN4I GGNNCC 3 cut(s) 132, 1074, 1298
PspPI GGNCC 1 cut(s) 821
PspXI VCTCGAGB 1 cut(s) 428
PstI CTGCAG 2 cut(s) 1066, 1120
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 3 cut(s) 130, 151, 436
PvuII CAGCTG 1 cut(s) 1235
RseI CAYNNNNRTG 1 cut(s) 991
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 4 cut(s) 207, 362, 491, 755
SatI GCNGC 6 cut(s) 203, 307, 1116, 1119, 1233, 1236
Sau3AI GATC 5 cut(s) 70, 130, 151, 232, 436
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 3 cut(s) 440, 692, 755
ScrFI CCNGG 3 cut(s) 567, 695, 1161
SduI GDGCHC 2 cut(s) 893, 1060
SfaNI GCATC 2 cut(s) 292, 1162
SfcI CTRYAG 2 cut(s) 1062, 1116
SfoI GGCGCC 1 cut(s) 1298
Sfr274I CTCGAG 1 cut(s) 428
SfuI TTCGAA 2 cut(s) 23, 47
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 991
SmlI CTYRAG 4 cut(s) 12, 428, 549, 734
SmoI CTYRAG 4 cut(s) 12, 428, 549, 734
SseBI AGGCCT 1 cut(s) 836
SsiI CCGC 5 cut(s) 304, 307, 1104, 1212, 1217
SspDI GGCGCC 1 cut(s) 1296
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 545
StuI AGGCCT 1 cut(s) 836
StyD4I CCNGG 3 cut(s) 565, 693, 1159
StyI CCWWGG 2 cut(s) 147, 868
TaaI ACNGT 2 cut(s) 624, 1003
TaiI ACGT 1 cut(s) 781
TaqI TCGA 6 cut(s) 23, 47, 188, 429, 902, 969
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 2 cut(s) 409, 460
Tru1I TTAA 4 cut(s) 207, 362, 491, 755
Tru9I TTAA 4 cut(s) 207, 362, 491, 755
TscAI CASTG 1 cut(s) 248
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 5 cut(s) 202, 1115, 1118, 1232, 1235
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 7 cut(s) 308, 402, 890, 923, 927, 941, 1052
TspRI CASTG 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 950
VneI GTGCAC 1 cut(s) 1056
XapI RAATTY 6 cut(s) 263, 473, 670, 676, 904, 961
XhoI CTCGAG 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.