Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005774

Physical Location & Seq

Genomic Coordinates Reverse (-)

5730664 .. 5738738

Gene Structure 8075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1032700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 558 bp

ATGGCGTCCGAACCGAAAGATTCTCCGGCCAACAATCCCGGCCTCCAGGCCACCCCAGATGAAGCCACTAGGGGTTACATCATGCAGCAAACTATGTTCCGAATTAAGGACCCGAAAGCGAGTCTCGACTTTTATTCACGTGTTTTGGGAATGTCGCTACTTAAGAGGCTCGATTTTCCAGAAATGAAGTTTAGTTTGTACTTTTTGGGCTATGAGGATCCTGCTTCAGCTCCAACTAACTCAGTTGATAGAACAGTTTGGACCTTTGGTCGGAAAGCTACAATTGAGTTGACACATAATTGGGGTACTGAAAGTGATTCAGAATTTAAAGGATATCACAATGGGAATTCAGAACCTCGTGGCTTTGGTCACATTGGCATCACCGTTGATGATACATACAAGGCCTGTGAGAGATTTGAAAGTCTGGGGGTGGAATTTGTGAAAAAGCCTGATGATGGAAAGATGAAAGGTATAGCATTCATTAAGGATCCTGATGGTTATTGGATTGAGATCTTTGACCTTAAAACTATAGGAACAGTCACAGCTGATGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00620 map00620

Pfam Domains

Protein Families

Glyoxalase

Protein Analysis

186

Amino Acids

20.74

Weight (kDa)

5.12

Isoelectric Point (pI)

35.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyoxalase	PF00903	29 - 171	8.1e-27	Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4	PF13669	30 - 153	1.4e-07	Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08110 AT1G08110 AT1G08110 AT1G08110
fragaria_vesca	FvH4_3g30960 FvH4_3g30960 FvH4_3g30960
malus_domestica	MD00G1032700.v1.1 MD11G1146100.v1.1
prunus_persica	Prupe.6G109100_v2.0.a1 Prupe.6G109100_v2.0.a1 Prupe.6G109100_v2.0.a1 Prupe.6G109100_v2.0.a1 Prupe.6G109100_v2.0.a1 Prupe.6G109100_v2.0.a1
pyrus_communis	pycom11g11910
rosa_chinensis	RchiOBHm_Chr5g0057511 RchiOBHm_Chr5g0057571
rosa_laevigata	RLG00000035161
rosa_multiflora	Rmu_sc0000508.1_g000002
rosa_roxburghii	Rroxscaffold_1G00022920
rosa_rugosa	Rorug05G0307800 Rorug05G0307900
rosa_samantha	Rh5AG376400 Rh5BG387200 Rh5CG410700 Rh5DG401400
rosa_wichuraiana	Rw5G035530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	4 cut(s)	212, 225, 482, 495
AcoI	YGGCCR	1 cut(s)	27
AcsI	RAATTY	3 cut(s)	323, 346, 434
AcuI	CTGAAG	1 cut(s)	210
AcvI	CACGTG	1 cut(s)	140
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	2 cut(s)	200, 307
AfiI	CCNNNNNNNGG	3 cut(s)	106, 270, 455
AflII	CTTAAG	1 cut(s)	161
AflIII	ACRYGT	1 cut(s)	139
AgsI	TTSAA	1 cut(s)	419
AhdI	GACNNNNNGTC	1 cut(s)	267
AjnI	CCWGG	1 cut(s)	45
AluBI	AGCT	3 cut(s)	230, 278, 545
AluI	AGCT	3 cut(s)	230, 278, 545
Alw26I	GTCTC	1 cut(s)	128
AlwI	GGATC	4 cut(s)	212, 225, 482, 495
AoxI	GGCC	4 cut(s)	27, 40, 48, 402
ApeKI	GCWGC	1 cut(s)	85
ApoI	RAATTY	3 cut(s)	323, 346, 434
AspS9I	GGNCC	2 cut(s)	109, 261
AsuC2I	CCSGG	1 cut(s)	39
AsuHPI	GGTGA	1 cut(s)	373
AvaII	GGWCC	2 cut(s)	109, 261
BamHI	GGATCC	2 cut(s)	217, 487
BauI	CACGAG	1 cut(s)	357
BbrPI	CACGTG	1 cut(s)	140
BbvI	GCAGC	1 cut(s)	97
BccI	CCATC	2 cut(s)	449, 488
BciT130I	CCWGG	1 cut(s)	47
BcnI	CCSGG	1 cut(s)	39
BcoDI	GTCTC	1 cut(s)	128
BfaI	CTAG	1 cut(s)	69
BfmI	CTRYAG	1 cut(s)	528
BfrI	CTTAAG	1 cut(s)	161
BglII	AGATCT	1 cut(s)	510
BisI	GCNGC	1 cut(s)	86
BlsI	GCNGC	1 cut(s)	87
Bme1390I	CCNGG	2 cut(s)	39, 47
Bme18I	GGWCC	2 cut(s)	109, 261
BmeRI	GACNNNNNGTC	1 cut(s)	267
BmgT120I	GGNCC	2 cut(s)	109, 261
BmiI	GGNNCC	3 cut(s)	111, 219, 489
BmrFI	CCNGG	2 cut(s)	39, 47
BmsI	GCATC	2 cut(s)	387, 538
BpmI	CTGGAG	1 cut(s)	29
BpuMI	CCSGG	1 cut(s)	39
BsaAI	YACGTR	1 cut(s)	140
BsaBI	GATNNNNATC	1 cut(s)	509
BsaHI	GRCGYC	1 cut(s)	5
Bsc4I	CCNNNNNNNGG	3 cut(s)	106, 270, 455
Bse8I	GATNNNNATC	1 cut(s)	509
BseBI	CCWGG	1 cut(s)	47
BseJI	GATNNNNATC	1 cut(s)	509
BseLI	CCNNNNNNNGG	3 cut(s)	106, 270, 455
BseMII	CTCAG	1 cut(s)	255
BseXI	GCAGC	1 cut(s)	97
BshFI	GGCC	4 cut(s)	29, 42, 50, 404
BsiSI	CCGG	2 cut(s)	26, 39
BslI	CCNNNNNNNGG	3 cut(s)	106, 270, 455
BsmAI	GTCTC	1 cut(s)	128
BsmI	GAATGC	1 cut(s)	476
BsnI	GGCC	4 cut(s)	29, 42, 50, 404
Bsp143I	GATC	3 cut(s)	217, 487, 510
BspANI	GGCC	4 cut(s)	29, 42, 50, 404
BspCNI	CTCAG	1 cut(s)	254
BspLI	GGNNCC	3 cut(s)	111, 219, 489
BspPI	GGATC	4 cut(s)	212, 225, 482, 495
BspTI	CTTAAG	1 cut(s)	161
BssMI	GATC	3 cut(s)	217, 487, 510
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	357
Bst2BI	CACGAG	1 cut(s)	357
Bst2UI	CCWGG	1 cut(s)	47
Bst4CI	ACNGT	3 cut(s)	256, 385, 538
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	161
BstBAI	YACGTR	1 cut(s)	140
BstDEI	CTNAG	1 cut(s)	241
BstKTI	GATC	3 cut(s)	220, 490, 513
BstMAI	GTCTC	1 cut(s)	128
BstMBI	GATC	3 cut(s)	217, 487, 510
BstNI	CCWGG	1 cut(s)	47
BstSCI	CCNGG	2 cut(s)	37, 45
BstSFI	CTRYAG	1 cut(s)	528
BstV1I	GCAGC	1 cut(s)	97
BstX2I	RGATCY	3 cut(s)	217, 487, 510
BstYI	RGATCY	3 cut(s)	217, 487, 510
BsuRI	GGCC	4 cut(s)	29, 42, 50, 404
Cfr13I	GGNCC	2 cut(s)	109, 261
Csp6I	GTAC	2 cut(s)	199, 306
CviAII	CATG	1 cut(s)	82
CviQI	GTAC	2 cut(s)	199, 306
DdeI	CTNAG	1 cut(s)	241
DpnI	GATC	3 cut(s)	219, 489, 512
DpnII	GATC	3 cut(s)	217, 487, 510
DraI	TTTAAA	1 cut(s)	328
DriI	GACNNNNNGTC	1 cut(s)	267
EaeI	YGGCCR	1 cut(s)	27
Eam1105I	GACNNNNNGTC	1 cut(s)	267
Eco147I	AGGCCT	1 cut(s)	404
Eco32I	GATATC	1 cut(s)	335
Eco47I	GGWCC	2 cut(s)	109, 261
Eco57I	CTGAAG	1 cut(s)	210
Eco72I	CACGTG	1 cut(s)	140
EcoO109I	RGGNCCY	1 cut(s)	109
EcoRI	GAATTC	1 cut(s)	346
EcoRII	CCWGG	1 cut(s)	45
EcoRV	GATATC	1 cut(s)	335
FaeI	CATG	1 cut(s)	85
FaiI	YATR	7 cut(s)	83, 95, 213, 297, 397, 473, 530
FatI	CATG	1 cut(s)	81
Fnu4HI	GCNGC	1 cut(s)	86
Fsp4HI	GCNGC	1 cut(s)	86
FspBI	CTAG	1 cut(s)	69
GluI	GCNGC	1 cut(s)	86
GsuI	CTGGAG	1 cut(s)	29
HaeIII	GGCC	4 cut(s)	29, 42, 50, 404
HapII	CCGG	2 cut(s)	26, 39
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	1 cut(s)	85
HincII	GTYRAC	1 cut(s)	291
HindII	GTYRAC	1 cut(s)	291
HinfI	GANTC	3 cut(s)	20, 121, 317
HpaII	CCGG	2 cut(s)	26, 39
HphI	GGTGA	1 cut(s)	373
Hpy166II	GTNNAC	1 cut(s)	291
Hpy188I	TCNGA	5 cut(s)	10, 101, 273, 322, 352
Hpy188III	TCNNGA	4 cut(s)	125, 179, 491, 555
Hpy8I	GTNNAC	1 cut(s)	291
HpyCH4III	ACNGT	3 cut(s)	256, 385, 538
HpyCH4IV	ACGT	1 cut(s)	139
HpyCH4V	TGCA	1 cut(s)	85
HpyF3I	CTNAG	1 cut(s)	241
HpySE526I	ACGT	1 cut(s)	139
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	1 cut(s)	85
Kzo9I	GATC	3 cut(s)	217, 487, 510
LmnI	GCTCC	1 cut(s)	235
Lsp1109I	GCAGC	1 cut(s)	97
LweI	GCATC	2 cut(s)	387, 538
MaeI	CTAG	1 cut(s)	69
MaeII	ACGT	1 cut(s)	139
MaeIII	GTNAC	3 cut(s)	74, 368, 538
MalI	GATC	3 cut(s)	219, 489, 512
MboI	GATC	3 cut(s)	217, 487, 510
MfeI	CAATTG	1 cut(s)	282
MflI	RGATCY	3 cut(s)	217, 487, 510
MluCI	AATT	6 cut(s)	102, 282, 298, 323, 346, 434
MlyI	GAGTC	1 cut(s)	130
MmeI	TCCRAC	2 cut(s)	251, 257
MnlI	CCTC	4 cut(s)	53, 159, 208, 366
MseI	TTAA	5 cut(s)	105, 162, 327, 483, 522
MspA1I	CMGCKG	1 cut(s)	545
MspCI	CTTAAG	1 cut(s)	161
MspI	CCGG	2 cut(s)	26, 39
MspR9I	CCNGG	2 cut(s)	39, 47
MunI	CAATTG	1 cut(s)	282
Mva1269I	GAATGC	1 cut(s)	476
MvaI	CCWGG	1 cut(s)	47
NciI	CCSGG	1 cut(s)	39
NdeII	GATC	3 cut(s)	217, 487, 510
NlaIII	CATG	1 cut(s)	85
NlaIV	GGNNCC	3 cut(s)	111, 219, 489
NmuCI	GTSAC	2 cut(s)	368, 538
PceI	AGGCCT	1 cut(s)	404
PctI	GAATGC	1 cut(s)	476
PfeI	GAWTC	2 cut(s)	20, 317
PkrI	GCNGC	1 cut(s)	87
PleI	GAGTC	1 cut(s)	129
PmaCI	CACGTG	1 cut(s)	140
PmlI	CACGTG	1 cut(s)	140
PpsI	GAGTC	1 cut(s)	129
Ppu21I	YACGTR	1 cut(s)	140
PpuMI	RGGWCCY	1 cut(s)	109
Psp5II	RGGWCCY	1 cut(s)	109
Psp6I	CCWGG	1 cut(s)	45
PspCI	CACGTG	1 cut(s)	140
PspGI	CCWGG	1 cut(s)	45
PspN4I	GGNNCC	3 cut(s)	111, 219, 489
PspPI	GGNCC	2 cut(s)	109, 261
PspPPI	RGGWCCY	1 cut(s)	109
PsuI	RGATCY	3 cut(s)	217, 487, 510
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	2 cut(s)	200, 307
RsaNI	GTAC	2 cut(s)	199, 306
SaqAI	TTAA	5 cut(s)	105, 162, 327, 483, 522
SatI	GCNGC	1 cut(s)	86
Sau3AI	GATC	3 cut(s)	217, 487, 510
Sau96I	GGNCC	2 cut(s)	109, 261
SchI	GAGTC	1 cut(s)	130
ScrFI	CCNGG	2 cut(s)	39, 47
SetI	ASST	8 cut(s)	142, 232, 266, 280, 358, 472, 522, 547
SfaNI	GCATC	2 cut(s)	387, 538
SfcI	CTRYAG	1 cut(s)	528
SinI	GGWCC	2 cut(s)	109, 261
SmlI	CTYRAG	1 cut(s)	161
SmoI	CTYRAG	1 cut(s)	161
Sse9I	AATT	6 cut(s)	102, 282, 298, 323, 346, 434
SseBI	AGGCCT	1 cut(s)	404
SspMI	CTAG	1 cut(s)	69
StuI	AGGCCT	1 cut(s)	404
StyD4I	CCNGG	2 cut(s)	37, 45
TaaI	ACNGT	3 cut(s)	256, 385, 538
TaiI	ACGT	1 cut(s)	142
TaqI	TCGA	2 cut(s)	126, 171
TasI	AATT	6 cut(s)	102, 282, 298, 323, 346, 434
TatI	WGTACW	1 cut(s)	198
TfiI	GAWTC	2 cut(s)	20, 317
Tru1I	TTAA	5 cut(s)	105, 162, 327, 483, 522
Tru9I	TTAA	5 cut(s)	105, 162, 327, 483, 522
TseFI	GTSAC	2 cut(s)	368, 538
TseI	GCWGC	1 cut(s)	85
Tsp45I	GTSAC	2 cut(s)	368, 538
TspDTI	ATGAA	4 cut(s)	75, 200, 469, 479
Vha464I	CTTAAG	1 cut(s)	161
VpaK11BI	GGWCC	2 cut(s)	109, 261
XapI	RAATTY	3 cut(s)	323, 346, 434
XspI	CTAG	1 cut(s)	69

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.