Rorug05G0307800

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005774
Physical Location & Seq
Reverse (-)
36033492 .. 36035620
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0307800.1

Sequence Viewer

Length: 1491 bp
ATGATGGTTTGGAATTGGGCAGTAATAATTGCATTGCTTGCTGTTGTTTATATTCTGGAACCATGGAGAAGCAAGAAGAAGAGGTTACCTCCTGGTCCAAGAGGGTTTCCTATTTTCGGAAGCCTCAACTTGTTAGGGGAGTTTCCTCACAAAGACCTACACAGACTGGCTCAAAAGTATGGCGACATCATGCACATGCGATTAGGTCTCGTGTCTGCCATCGTCGTCTCCTCCCCTCAAGCAGCCGAGCTCTTCCTCAAGACCCACGACCTTGTTTTCGCAAGCAGGCCACCTCACGAAGGCGCAAAGCACATCTCTTTCGGGCAGAGGAACTTATCCTTTTCCGAGTATGGCTCTTATTGGCGAGACATGCGCAAGATGTGCACCCTCGAGTTGCTCAGCAACCACAAAATCAATTCTTTCAAGGAAATGAGGAGAGAAGAGGTTGTCCTTTTCATAGAGGCCATTAAAGAGGCAGCTGCAACCAGCCACGAACCAGTCGATCTCAGCGCTAAGGTGTCATCTCTTAGTGCAGATATGAGCTGCAGGATGGTGTTTGGAAAGAAGTACGCGGATAAAGAATTTGACGACAAGGGTTTCAAGTCTGTGATTCAACAAGCCATACAATTAGCAGCTGCGCCTAACTTGGGCGATTACATCCCTTGTATTGCTCCACTTGACCTCCAAGGGTTCACTAAGCGCATGAAGGCTATTAACAAGGTGTTTGATGACTTTTTCGAGAAGATTATTGATGAACATCTTCAATCGAGGGATGAGGGAAAGACGAAGGACTTTGTTGATGTAATGCTCAGCTTTATGGGGACTCAAGAATCTGAATACCTAATTGAACGCTCCAATATCAAGGCAATCATTTTTGACATGCTTGTGGCCTCAATGGACACATCAGCAACAACAATTGAGTGGGCCGTCTCTGAACTCATAAAACATCCACAACTAATGAAGAAGGTCCGAAAAGAGCTAGAAAATGGGGTGGGAATGGAGAGAATGGTCGAGGAATCAGACTTGGACAAGTTGGAGTACCTGGACATGGTAGTGAAAGAAACCTTGAGGCTACATCCAGTGGGACCTTTGTTGCTTCCCCATGCATCCACTGAAGATTGCACTGTTAACGGCTTCCACATACCCAAAAAATCACGCATTATCATAAACGTGTGGGCAATTGGAAGAGACCCCAATGCTTGGACCAATGCAGACGAGTTCATACCCGAAAGGTTTGCAGGGAGTAATATAGACCTGAGGGGAAATCACTTTCAGCTTATTCCATTTGGTTCGGGTCGTAGACGTTGCCCTGGAATTCAATTGGGGCTAGTTGTGGTCAAGCTAGTATTGGCACAACTTCTGCACTGTTTCGATTGGGAGCTTCTGGATGGTTTGTTGCCAACAGAGTTGGACATGACGGAGGAATTTGGTCTAACCGTTTCACGAGCCAAGCATCTGCTGGTTGTTCCTTCTTATCGCCTTCACAAATAA

Protein Analysis

496

Amino Acids

56.39

Weight (kDa)

6.8

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 484 5e-107 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
AccB7I CCANNNNNTGG 1 cut(s) 1200
AccI GTMKAC 1 cut(s) 1300
AccII CGCG 1 cut(s) 572
AciI CCGC 1 cut(s) 572
AcsI RAATTY 3 cut(s) 581, 1314, 1424
AcuI CTGAAG 1 cut(s) 1134
AfaI GTAC 2 cut(s) 569, 1040
AfeI AGCGCT 1 cut(s) 511
AfiI CCNNNNNNNGG 5 cut(s) 116, 299, 647, 1048, 1200
AflIII ACRYGT 1 cut(s) 1170
AgsI TTSAA 6 cut(s) 424, 601, 614, 764, 848, 1319
AjnI CCWGG 3 cut(s) 91, 1041, 1309
AluBI AGCT 9 cut(s) 250, 479, 543, 635, 813, 979, 1276, 1342, 1381
AluI AGCT 9 cut(s) 250, 479, 543, 635, 813, 979, 1276, 1342, 1381
Alw21I GWGCWC 2 cut(s) 252, 386
Alw26I GTCTC 5 cut(s) 212, 232, 360, 934, 1182
Alw44I GTGCAC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 511
AoxI GGCC 4 cut(s) 287, 462, 888, 924
ApaLI GTGCAC 1 cut(s) 382
ApeKI GCWGC 6 cut(s) 242, 476, 479, 543, 632, 635
ApoI RAATTY 3 cut(s) 581, 1314, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 5 cut(s) 305, 375, 512, 640, 702
AspS9I GGNCC 5 cut(s) 95, 924, 967, 1085, 1203
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 4 cut(s) 95, 967, 1085, 1203
AxyI CCTNAGG 1 cut(s) 1256
BaeGI GKGCMC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 2 cut(s) 209, 1443
Bbv12I GWGCWC 2 cut(s) 252, 386
BbvI GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
BccI CCATC 3 cut(s) 227, 544, 1382
BceAI ACGGC 2 cut(s) 911, 1147
BcgI CGANNNNNNTGC 2 cut(s) 1217, 1251
BciT130I CCWGG 3 cut(s) 93, 1043, 1311
BcoDI GTCTC 5 cut(s) 212, 232, 360, 934, 1182
BfaI CTAG 3 cut(s) 980, 1328, 1343
BfmI CTRYAG 1 cut(s) 544
BfoI RGCGCY 1 cut(s) 513
BisI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
BlpI GCTNAGC 2 cut(s) 398, 809
BlsI GCNGC 6 cut(s) 244, 478, 481, 545, 634, 637
Bme1390I CCNGG 3 cut(s) 93, 1043, 1311
Bme18I GGWCC 4 cut(s) 95, 967, 1085, 1203
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 5 cut(s) 95, 924, 967, 1085, 1203
BmiI GGNNCC 2 cut(s) 60, 1086
BmrFI CCNGG 3 cut(s) 93, 1043, 1311
BmsI GCATC 2 cut(s) 1115, 1462
BplI GAGNNNNNCTC 4 cut(s) 73, 105, 338, 370
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 2 cut(s) 398, 809
BpuEI CTTGAG 4 cut(s) 222, 242, 810, 1087
BsaBI GATNNNNATC 1 cut(s) 756
BsaI GGTCTC 2 cut(s) 212, 1182
BsaJI CCNNGG 3 cut(s) 62, 685, 1309
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 299, 647, 1048, 1200
Bse1I ACTGG 3 cut(s) 171, 497, 1079
Bse21I CCTNAGG 1 cut(s) 1256
Bse3DI GCAATG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 3 cut(s) 93, 1043, 1311
BseDI CCNNGG 3 cut(s) 62, 685, 1309
BseGI GGATG 7 cut(s) 555, 657, 778, 946, 1075, 1106, 1393
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 5 cut(s) 116, 299, 647, 1048, 1200
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 4 cut(s) 412, 520, 823, 1247
BseNI ACTGG 3 cut(s) 171, 497, 1079
BseRI GAGGAG 2 cut(s) 220, 448
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
BsgI GTGCAG 2 cut(s) 552, 1346
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 4 cut(s) 289, 464, 890, 926
BsiHKAI GWGCWC 2 cut(s) 252, 386
BsiHKCI CYCGRG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 835, 1098
BslI CCNNNNNNNGG 5 cut(s) 116, 299, 647, 1048, 1200
BsmAI GTCTC 5 cut(s) 212, 232, 360, 934, 1182
BsmBI CGTCTC 2 cut(s) 232, 934
BsmFI GGGAC 2 cut(s) 835, 1098
BsnI GGCC 4 cut(s) 289, 464, 890, 926
Bso31I GGTCTC 2 cut(s) 212, 1182
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 252, 386
Bsp143I GATC 1 cut(s) 502
Bsp1720I GCTNAGC 2 cut(s) 398, 809
Bsp19I CCATGG 1 cut(s) 62
BspACI CCGC 1 cut(s) 572
BspANI GGCC 4 cut(s) 289, 464, 890, 926
BspCNI CTCAG 4 cut(s) 411, 519, 822, 1248
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 2 cut(s) 60, 1086
BspMAI CTGCAG 1 cut(s) 548
BspQI GCTCTTC 1 cut(s) 257
BspTNI GGTCTC 2 cut(s) 212, 1182
BsrDI GCAATG 1 cut(s) 32
BsrI ACTGG 3 cut(s) 171, 497, 1079
BssECI CCNNGG 3 cut(s) 62, 685, 1309
BssMI GATC 1 cut(s) 502
BssSI CACGAG 2 cut(s) 209, 1443
BssT1I CCWWGG 2 cut(s) 62, 685
Bst2BI CACGAG 2 cut(s) 209, 1443
Bst2UI CCWGG 3 cut(s) 93, 1043, 1311
Bst4CI ACNGT 3 cut(s) 1126, 1367, 1438
Bst6I CTCTTC 4 cut(s) 74, 257, 435, 1180
BstAPI GCANNNNNTGC 2 cut(s) 38, 381
BstC8I GCNNGC 3 cut(s) 39, 283, 287
BstDEI CTNAG 7 cut(s) 398, 506, 513, 527, 696, 809, 1256
BstDSI CCRYGG 1 cut(s) 62
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 7 cut(s) 555, 657, 778, 946, 1075, 1106, 1393
BstFNI CGCG 1 cut(s) 572
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 5 cut(s) 305, 375, 512, 640, 702
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 5 cut(s) 212, 232, 360, 934, 1182
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 3 cut(s) 38, 370, 381
BstNI CCWGG 3 cut(s) 93, 1043, 1311
BstNSI RCATGY 3 cut(s) 199, 373, 883
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 91, 1041, 1309
BstSFI CTRYAG 1 cut(s) 544
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
Bsu36I CCTNAGG 1 cut(s) 1256
BsuRI GGCC 4 cut(s) 289, 464, 890, 926
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 7 cut(s) 555, 657, 778, 946, 1075, 1106, 1393
BtsIMutI CAGTG 4 cut(s) 1086, 1110, 1122, 1363
Cac8I GCNNGC 3 cut(s) 39, 283, 287
CfoI GCGC 5 cut(s) 305, 375, 512, 640, 702
Cfr13I GGNCC 5 cut(s) 95, 924, 967, 1085, 1203
Csp6I GTAC 2 cut(s) 568, 1039
CviAII CATG 9 cut(s) 63, 190, 196, 370, 703, 880, 1048, 1103, 1414
CviQI GTAC 2 cut(s) 568, 1039
DdeI CTNAG 7 cut(s) 398, 506, 513, 527, 696, 809, 1256
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eam1104I CTCTTC 4 cut(s) 74, 257, 435, 1180
EarI CTCTTC 4 cut(s) 74, 257, 435, 1180
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 62, 685
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 2 cut(s) 212, 1182
Eco47I GGWCC 4 cut(s) 95, 967, 1085, 1203
Eco47III AGCGCT 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 1134
Eco81I CCTNAGG 1 cut(s) 1256
Eco88I CYCGRG 1 cut(s) 389
Eco91I GGTNACC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 1085
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 1314
EcoRII CCWGG 3 cut(s) 91, 1041, 1309
EcoT14I CCWWGG 2 cut(s) 62, 685
EcoT22I ATGCAT 1 cut(s) 1108
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 2 cut(s) 62, 685
Esp3I CGTCTC 2 cut(s) 232, 934
FaeI CATG 9 cut(s) 66, 193, 199, 373, 706, 883, 1051, 1106, 1417
FaqI GGGAC 2 cut(s) 835, 1098
FatI CATG 9 cut(s) 62, 189, 195, 369, 702, 879, 1047, 1102, 1413
FblI GTMKAC 1 cut(s) 1300
Fnu4HI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
FokI GGATG 7 cut(s) 562, 644, 785, 933, 1062, 1093, 1400
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
FspBI CTAG 3 cut(s) 980, 1328, 1343
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 5 cut(s) 304, 374, 511, 639, 701
GluI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 4 cut(s) 289, 464, 890, 926
HhaI GCGC 5 cut(s) 305, 375, 512, 640, 702
Hin1II CATG 9 cut(s) 66, 193, 199, 373, 706, 883, 1051, 1106, 1417
Hin6I GCGC 5 cut(s) 303, 373, 510, 638, 700
HinP1I GCGC 5 cut(s) 303, 373, 510, 638, 700
HincII GTYRAC 1 cut(s) 1129
HindII GTYRAC 1 cut(s) 1129
HinfI GANTC 4 cut(s) 610, 823, 830, 1016
HpaI GTTAAC 1 cut(s) 1129
Hpy166II GTNNAC 4 cut(s) 384, 693, 1129, 1301
Hpy188I TCNGA 6 cut(s) 119, 346, 835, 934, 971, 1021
Hpy188III TCNNGA 7 cut(s) 56, 259, 296, 739, 827, 1385, 1443
Hpy8I GTNNAC 4 cut(s) 384, 693, 1129, 1301
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 6 cut(s) 293, 700, 781, 958, 1479, 1490
HpyCH4III ACNGT 3 cut(s) 1126, 1367, 1438
HpyCH4IV ACGT 2 cut(s) 1170, 1303
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 370, 381
HpyF3I CTNAG 7 cut(s) 398, 506, 513, 527, 696, 809, 1256
HpySE526I ACGT 2 cut(s) 1170, 1303
Hsp92II CATG 9 cut(s) 66, 193, 199, 373, 706, 883, 1051, 1106, 1417
HspAI GCGC 5 cut(s) 303, 373, 510, 638, 700
KspAI GTTAAC 1 cut(s) 1129
Kzo9I GATC 1 cut(s) 502
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 3 cut(s) 676, 857, 1378
Lsp1109I GCAGC 6 cut(s) 254, 466, 488, 530, 622, 644
LweI GCATC 2 cut(s) 1115, 1462
MaeI CTAG 3 cut(s) 980, 1328, 1343
MaeII ACGT 2 cut(s) 1170, 1303
MaeIII GTNAC 1 cut(s) 84
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 9 cut(s) 88, 91, 244, 452, 752, 754, 973, 1127, 1197
MfeI CAATTG 3 cut(s) 915, 1179, 1319
MhlI GDGCHC 2 cut(s) 252, 386
MlyI GAGTC 1 cut(s) 817
MmeI TCCRAC 2 cut(s) 1014, 1389
Mph1103I ATGCAT 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 3 cut(s) 468, 714, 1128
MslI CAYNNNNRTG 4 cut(s) 194, 884, 1052, 1169
MspA1I CMGCKG 2 cut(s) 479, 635
MspR9I CCNGG 3 cut(s) 93, 1043, 1311
MunI CAATTG 3 cut(s) 915, 1179, 1319
MvaI CCWGG 3 cut(s) 93, 1043, 1311
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 3 cut(s) 38, 370, 381
NcoI CCATGG 1 cut(s) 62
NdeII GATC 1 cut(s) 502
NlaIII CATG 9 cut(s) 66, 193, 199, 373, 706, 883, 1051, 1106, 1417
NlaIV GGNNCC 2 cut(s) 60, 1086
NmeAIII GCCGAG 1 cut(s) 271
NsbI TGCGCA 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 1108
NspI RCATGY 3 cut(s) 199, 373, 883
PaeR7I CTCGAG 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 3 cut(s) 610, 830, 1016
PflMI CCANNNNNTGG 1 cut(s) 1200
PkrI GCNGC 6 cut(s) 244, 478, 481, 545, 634, 637
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
PpuMI RGGWCCY 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 1085
Psp6I CCWGG 3 cut(s) 91, 1041, 1309
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 3 cut(s) 91, 1041, 1309
PspN4I GGNNCC 2 cut(s) 60, 1086
PspPI GGNCC 5 cut(s) 95, 924, 967, 1085, 1203
PspPPI RGGWCCY 1 cut(s) 1085
PspXI VCTCGAGB 1 cut(s) 389
PstI CTGCAG 1 cut(s) 548
PvuII CAGCTG 2 cut(s) 479, 635
RsaI GTAC 2 cut(s) 569, 1040
RsaNI GTAC 2 cut(s) 568, 1039
RseI CAYNNNNRTG 4 cut(s) 194, 884, 1052, 1169
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 3 cut(s) 468, 714, 1128
SatI GCNGC 6 cut(s) 243, 477, 480, 544, 633, 636
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 5 cut(s) 95, 924, 967, 1085, 1203
SchI GAGTC 1 cut(s) 817
ScrFI CCNGG 3 cut(s) 93, 1043, 1311
SduI GDGCHC 2 cut(s) 252, 386
SfaNI GCATC 2 cut(s) 1115, 1462
SfcI CTRYAG 1 cut(s) 544
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 4 cut(s) 95, 967, 1085, 1203
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 4 cut(s) 194, 884, 1052, 1169
SmlI CTYRAG 5 cut(s) 237, 257, 389, 825, 1066
SmoI CTYRAG 5 cut(s) 237, 257, 389, 825, 1066
SsiI CCGC 1 cut(s) 572
SspMI CTAG 3 cut(s) 980, 1328, 1343
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 91, 1041, 1309
StyI CCWWGG 2 cut(s) 62, 685
TaaI ACNGT 3 cut(s) 1126, 1367, 1438
TaiI ACGT 2 cut(s) 1173, 1306
TaqI TCGA 6 cut(s) 390, 501, 738, 767, 1011, 1371
TfiI GAWTC 3 cut(s) 610, 830, 1016
Tru1I TTAA 3 cut(s) 468, 714, 1128
Tru9I TTAA 3 cut(s) 468, 714, 1128
TscAI CASTG 4 cut(s) 1086, 1117, 1129, 1370
TseI GCWGC 6 cut(s) 242, 476, 479, 543, 632, 635
TspDTI ATGAA 5 cut(s) 445, 719, 768, 974, 1210
TspGWI ACGGA 1 cut(s) 1433
TspRI CASTG 4 cut(s) 1086, 1117, 1129, 1370
Van91I CCANNNNNTGG 1 cut(s) 1200
VneI GTGCAC 1 cut(s) 382
VpaK11BI GGWCC 4 cut(s) 95, 967, 1085, 1203
XagI CCTNNNNNAGG 1 cut(s) 297
XapI RAATTY 3 cut(s) 581, 1314, 1424
XceI RCATGY 3 cut(s) 199, 373, 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XhoI CTCGAG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 1300
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 3 cut(s) 980, 1328, 1343
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.