MD00G1058900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008015
Physical Location & Seq
Reverse (-)
10720267 .. 10722919
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1058900.v1.1.491

Sequence Viewer

Length: 318 bp
ATGCAGGAGGAGGAGGAGACTCCGGACAATGGAAGCGGCGGTAGCGCTGGGTCACACACCCACTTGTGGTGGGCCGGGGCCAGTGCGGCTCAGCTGGGTTGGGCCGTTATGTCATCCCGGAAAGGCTGCGCCGGTAACTCCTTTACGATGCCTTTCAAGGCCTTCGCCGTTGCTTCCCTCTACGTCGGCTCTGTGGCCACCGCCGGCGTCGCTGGCCTCCATGCCTCTGGCATCCGCGAGGTGGAGGATCTAGTAGAGCTGGGTGCCAACATAAGAACTGGACTTGGGGTACCCCGGAGGAGGTCGAGGGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

10.92

Weight (kDa)

5.89

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 289
AccB1I GGYRCC 2 cut(s) 263, 289
AccII CGCG 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 5 cut(s) 36, 39, 86, 201, 235
AclWI GGATC 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 195
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 291
AfeI AGCGCT 1 cut(s) 46
AfiI CCNNNNNNNGG 4 cut(s) 29, 66, 241, 300
AgsI TTSAA 1 cut(s) 157
AluBI AGCT 2 cut(s) 94, 259
AluI AGCT 2 cut(s) 94, 259
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 22
Aor51HI AGCGCT 1 cut(s) 46
AoxI GGCC 6 cut(s) 72, 78, 102, 159, 195, 214
ApeKI GCWGC 1 cut(s) 126
Asp718I GGTACC 1 cut(s) 289
AspLEI GCGC 2 cut(s) 47, 131
AspS9I GGNCC 3 cut(s) 72, 78, 102
AsuC2I CCSGG 3 cut(s) 76, 118, 295
BalI TGGCCA 1 cut(s) 197
BanI GGYRCC 2 cut(s) 263, 289
BbvI GCAGC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 89, 152
BcnI CCSGG 3 cut(s) 76, 118, 295
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 48
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 3 cut(s) 37, 87, 127
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 3 cut(s) 38, 88, 128
Bme1390I CCNGG 3 cut(s) 76, 118, 295
BmgT120I GGNCC 3 cut(s) 72, 78, 102
BmiI GGNNCC 3 cut(s) 79, 265, 291
BmrFI CCNGG 3 cut(s) 76, 118, 295
BmsI GCATC 2 cut(s) 138, 240
Bpu1102I GCTNAGC 1 cut(s) 90
BpuMI CCSGG 3 cut(s) 76, 118, 295
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 75, 293
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 66, 241, 300
Bse118I RCCGGY 2 cut(s) 131, 203
Bse1I ACTGG 2 cut(s) 81, 283
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 2 cut(s) 75, 293
BseGI GGATG 2 cut(s) 113, 231
BseLI CCNNNNNNNGG 4 cut(s) 29, 66, 241, 300
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 81, 283
BseRI GAGGAG 4 cut(s) 23, 26, 29, 313
BseXI GCAGC 1 cut(s) 113
BseYI CCCAGC 3 cut(s) 47, 94, 259
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 6 cut(s) 74, 80, 104, 161, 197, 216
BshNI GGYRCC 2 cut(s) 263, 289
BsiSI CCGG 6 cut(s) 23, 75, 118, 132, 204, 295
BslI CCNNNNNNNGG 4 cut(s) 29, 66, 241, 300
BsmAI GTCTC 1 cut(s) 11
BsnI GGCC 6 cut(s) 74, 80, 104, 161, 197, 216
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 247
Bsp1720I GCTNAGC 1 cut(s) 90
BspACI CCGC 5 cut(s) 36, 39, 86, 201, 235
BspANI GGCC 6 cut(s) 74, 80, 104, 161, 197, 216
BspCNI CTCAG 1 cut(s) 103
BspEI TCCGGA 1 cut(s) 22
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 3 cut(s) 79, 265, 291
BspPI GGATC 1 cut(s) 255
BspT107I GGYRCC 2 cut(s) 263, 289
BsrFI RCCGGY 2 cut(s) 131, 203
BsrI ACTGG 2 cut(s) 81, 283
BssAI RCCGGY 2 cut(s) 131, 203
BssECI CCNNGG 2 cut(s) 75, 293
BssMI GATC 1 cut(s) 247
BssNI GRCGYC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 205, 214
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 113, 231
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 47, 131
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 4 cut(s) 42, 86, 209, 213
BstSCI CCNGG 3 cut(s) 74, 116, 293
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 247
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 6 cut(s) 74, 80, 104, 161, 197, 216
BtsCI GGATG 2 cut(s) 113, 231
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 2 cut(s) 205, 214
CfoI GCGC 2 cut(s) 47, 131
Cfr10I RCCGGY 2 cut(s) 131, 203
Cfr13I GGNCC 3 cut(s) 72, 78, 102
CseI GACGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 290
CviAII CATG 1 cut(s) 221
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 1 cut(s) 90
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 195
Eco147I AGGCCT 1 cut(s) 161
Eco47III AGCGCT 1 cut(s) 46
FaeI CATG 1 cut(s) 224
FaiI YATR 3 cut(s) 110, 222, 272
FatI CATG 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 37, 87, 127
FokI GGATG 2 cut(s) 100, 218
Fsp4HI GCNGC 3 cut(s) 37, 87, 127
FspBI CTAG 1 cut(s) 251
GlaI GCGC 2 cut(s) 46, 130
GluI GCNGC 3 cut(s) 37, 87, 127
GsaI CCCAGC 3 cut(s) 51, 98, 263
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 6 cut(s) 74, 80, 104, 161, 197, 216
HapII CCGG 6 cut(s) 23, 75, 118, 132, 204, 295
HgaI GACGC 1 cut(s) 196
HhaI GCGC 2 cut(s) 47, 131
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 224
Hin6I GCGC 2 cut(s) 45, 129
HinP1I GCGC 2 cut(s) 45, 129
HinfI GANTC 1 cut(s) 19
HpaII CCGG 6 cut(s) 23, 75, 118, 132, 204, 295
Hpy188III TCNNGA 1 cut(s) 23
Hpy99I CGWCG 2 cut(s) 188, 212
HpyAV CCTTC 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 86, 209, 213
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 224
HspAI GCGC 2 cut(s) 45, 129
Kpn2I TCCGGA 1 cut(s) 22
KpnI GGTACC 1 cut(s) 293
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 247
Lsp1109I GCAGC 1 cut(s) 113
LweI GCATC 2 cut(s) 138, 240
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 51, 134
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MflI RGATCY 1 cut(s) 247
MlsI TGGCCA 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 13
Mox20I TGGCCA 1 cut(s) 197
MreI CGCCGGCG 1 cut(s) 203
MroI TCCGGA 1 cut(s) 22
MroNI GCCGGC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 197
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 6 cut(s) 23, 75, 118, 132, 204, 295
MspR9I CCNGG 3 cut(s) 76, 118, 295
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 42, 86, 209, 213
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 3 cut(s) 76, 118, 295
NdeII GATC 1 cut(s) 247
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 3 cut(s) 79, 265, 291
NmuCI GTSAC 1 cut(s) 51
PceI AGGCCT 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 205
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 3 cut(s) 38, 88, 128
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspFI CCCAGC 3 cut(s) 47, 94, 259
PspN4I GGNNCC 3 cut(s) 79, 265, 291
PspPI GGNCC 3 cut(s) 72, 78, 102
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 94
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
SatI GCNGC 3 cut(s) 37, 87, 127
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 3 cut(s) 72, 78, 102
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 3 cut(s) 76, 118, 295
SetI ASST 5 cut(s) 96, 186, 243, 261, 305
SfaNI GCATC 2 cut(s) 138, 240
SgrAI CRCCGGYG 1 cut(s) 203
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 5 cut(s) 36, 39, 86, 201, 235
SspMI CTAG 1 cut(s) 251
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 74, 116, 293
TaiI ACGT 1 cut(s) 186
TaqI TCGA 1 cut(s) 305
TauI GCSGC 2 cut(s) 39, 89
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 51
TspRI CASTG 1 cut(s) 88
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.