MD00G1091900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008605
Physical Location & Seq
Forward (+)
19324137 .. 19324631
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1091900.v1.1.491

Sequence Viewer

Length: 495 bp
ATGCCAGGTGCCGGAGGTTCATTCATCTGCCTCATCTCTTGCGTTTTATTCATCTCTATCGTGGCCGGAGGCGGCTGCCTATTGATGTACATGATCCTACCTGAACCTCAAACCACTTGGCTTCCCATTACCGGAGTCGCGTTGGTTTGCCTTCCATGGTTGTTTTGGTTGCTCACATTCCTATATCGACTAATCTCTCGGGGCTCCGGATTCAGAGGTGGCATTGGCAATGCTGCTGCTGTGGCTGGTGGTGTAAAGGTTACTAACACAGCTGCATCGGCATCAAATTTACGTGCCAGTACCGATCTTGCTGCACCTTCTGCTCAATCGCCAGGGGGTGACACTGGTGCTATGGTAGCAAGAGAGGTAGAATTAGGAAGTTTTAAGGATGGAAAGCGAAGGATCTCGTCGTTAAGTCGCAATTCATCAAACATTAATGTATCTCGTCATTCTCATGAGAGTGAAATGCCTTTGGCCTTATCAATGGCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

17.03

Weight (kDa)

9.16

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 1 cut(s) 140
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 1 cut(s) 72
AclWI GGATC 2 cut(s) 88, 410
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 2 cut(s) 89, 301
AfiI CCNNNNNNNGG 2 cut(s) 11, 131
AjnI CCWGG 2 cut(s) 4, 331
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
AlwI GGATC 2 cut(s) 88, 410
Ama87I CYCGRG 1 cut(s) 198
Aor13HI TCCGGA 1 cut(s) 206
AoxI GGCC 2 cut(s) 63, 474
ApeKI GCWGC 5 cut(s) 75, 233, 236, 272, 311
ApoI RAATTY 1 cut(s) 286
AseI ATTAAT 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 350
AvaI CYCGRG 1 cut(s) 198
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 206
BbvI GCAGC 5 cut(s) 62, 220, 223, 259, 298
BccI CCATC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 2 cut(s) 6, 333
BisI GCNGC 6 cut(s) 73, 76, 234, 237, 273, 312
BlsI GCNGC 6 cut(s) 74, 77, 235, 238, 274, 313
Bme1390I CCNGG 2 cut(s) 6, 333
BmeT110I CYCGRG 1 cut(s) 198
BmiI GGNNCC 2 cut(s) 10, 205
BmrFI CCNGG 2 cut(s) 6, 333
BmsI GCATC 2 cut(s) 284, 290
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 155, 332
BsaWI WCCGGW 2 cut(s) 131, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 131
Bse1I ACTGG 2 cut(s) 297, 349
Bse3DI GCAATG 1 cut(s) 235
BseAI TCCGGA 1 cut(s) 206
BseBI CCWGG 2 cut(s) 6, 333
BseDI CCNNGG 2 cut(s) 155, 332
BseGI GGATG 2 cut(s) 394, 488
BseLI CCNNNNNNNGG 2 cut(s) 11, 131
BseMI GCAATG 1 cut(s) 235
BseNI ACTGG 2 cut(s) 297, 349
BseXI GCAGC 5 cut(s) 62, 220, 223, 259, 298
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 2 cut(s) 65, 476
BshNI GGYRCC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 12, 66, 132, 207
BslI CCNNNNNNNGG 2 cut(s) 11, 131
BsnI GGCC 2 cut(s) 65, 476
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 206
Bsp13I TCCGGA 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 3 cut(s) 93, 304, 402
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 1 cut(s) 72
BspANI GGCC 2 cut(s) 65, 476
BspEI TCCGGA 1 cut(s) 206
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 10, 205
BspPI GGATC 2 cut(s) 88, 410
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 2 cut(s) 297, 349
BssECI CCNNGG 2 cut(s) 155, 332
BssMI GATC 3 cut(s) 93, 304, 402
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 6, 333
BstAPI GCANNNNNTGC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 293
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 394, 488
BstFNI CGCG 1 cut(s) 140
BstKTI GATC 3 cut(s) 96, 307, 405
BstMBI GATC 3 cut(s) 93, 304, 402
BstMWI GCNNNNNNNGC 4 cut(s) 242, 278, 320, 356
BstNI CCWGG 2 cut(s) 6, 333
BstSCI CCNGG 2 cut(s) 4, 331
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 5 cut(s) 62, 220, 223, 259, 298
BstX2I RGATCY 1 cut(s) 402
BstYI RGATCY 1 cut(s) 402
BsuRI GGCC 2 cut(s) 65, 476
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 2 cut(s) 394, 488
BtsIMutI CAGTG 1 cut(s) 342
CciI TCATGA 1 cut(s) 454
Csp6I GTAC 2 cut(s) 88, 300
CviAII CATG 3 cut(s) 91, 156, 455
CviJI RGCY 7 cut(s) 65, 75, 121, 204, 245, 272, 476
CviKI_1 RGCY 7 cut(s) 65, 75, 121, 204, 245, 272, 476
CviQI GTAC 2 cut(s) 88, 300
DpnI GATC 3 cut(s) 95, 306, 404
DpnII GATC 3 cut(s) 93, 304, 402
EaeI YGGCCR 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 4, 331
EcoT14I CCWWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 3 cut(s) 94, 159, 458
FaiI YATR 5 cut(s) 92, 157, 184, 353, 456
FatI CATG 3 cut(s) 90, 155, 454
Fnu4HI GCNGC 6 cut(s) 73, 76, 234, 237, 273, 312
FokI GGATG 2 cut(s) 401, 475
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 6 cut(s) 73, 76, 234, 237, 273, 312
GluI GCNGC 6 cut(s) 73, 76, 234, 237, 273, 312
HaeIII GGCC 2 cut(s) 65, 476
HapII CCGG 4 cut(s) 12, 66, 132, 207
Hin1II CATG 3 cut(s) 94, 159, 458
HinfI GANTC 2 cut(s) 135, 210
HpaII CCGG 4 cut(s) 12, 66, 132, 207
HphI GGTGA 1 cut(s) 350
Hpy188I TCNGA 1 cut(s) 215
Hpy188III TCNNGA 3 cut(s) 207, 455, 492
Hpy99I CGWCG 1 cut(s) 412
HpyAV CCTTC 3 cut(s) 161, 327, 393
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 275, 314
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 278, 320, 356
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 3 cut(s) 94, 159, 458
Kpn2I TCCGGA 1 cut(s) 206
Kzo9I GATC 3 cut(s) 93, 304, 402
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 5 cut(s) 62, 220, 223, 259, 298
LweI GCATC 2 cut(s) 284, 290
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 259, 338
MalI GATC 3 cut(s) 95, 306, 404
MboI GATC 3 cut(s) 93, 304, 402
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 3 cut(s) 286, 371, 421
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 6 cut(s) 8, 41, 62, 117, 209, 358
MroI TCCGGA 1 cut(s) 206
MseI TTAA 3 cut(s) 384, 413, 435
MslI CAYNNNNRTG 2 cut(s) 453, 459
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 4 cut(s) 12, 66, 132, 207
MspR9I CCNGG 2 cut(s) 6, 333
MvaI CCWGG 2 cut(s) 6, 333
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 4 cut(s) 242, 278, 320, 356
NcoI CCATGG 1 cut(s) 155
NdeII GATC 3 cut(s) 93, 304, 402
NlaIII CATG 3 cut(s) 94, 159, 458
NlaIV GGNNCC 2 cut(s) 10, 205
NmuCI GTSAC 1 cut(s) 338
PagI TCATGA 1 cut(s) 454
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 6 cut(s) 74, 77, 235, 238, 274, 313
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 4, 331
PspGI CCWGG 2 cut(s) 4, 331
PspN4I GGNNCC 2 cut(s) 10, 205
PsuI RGATCY 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 2 cut(s) 89, 301
RsaNI GTAC 2 cut(s) 88, 300
RseI CAYNNNNRTG 2 cut(s) 453, 459
SaqAI TTAA 3 cut(s) 384, 413, 435
SatI GCNGC 6 cut(s) 73, 76, 234, 237, 273, 312
Sau3AI GATC 3 cut(s) 93, 304, 402
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 2 cut(s) 6, 333
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 2 cut(s) 284, 290
SmiMI CAYNNNNRTG 2 cut(s) 453, 459
Sse9I AATT 3 cut(s) 286, 371, 421
SsiI CCGC 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 4, 331
StyI CCWWGG 1 cut(s) 155
TaiI ACGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 187
TasI AATT 3 cut(s) 286, 371, 421
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 3 cut(s) 384, 413, 435
Tru9I TTAA 3 cut(s) 384, 413, 435
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 5 cut(s) 75, 233, 236, 272, 311
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 4 cut(s) 9, 13, 40, 414
TspRI CASTG 1 cut(s) 349
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.