Prupe.4G274100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008605
Physical Location & Seq
Forward (+)
22820891 .. 22822007
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G274100.1

Sequence Viewer

Length: 543 bp
ATGAAAAAACTGAAGGGTGGTGATCAAATGAATCGTGCCCCAGGAGGTTCGTTCATATGTTTCATCTCTTGCTTTTTATTCGTATCCATCGTGGCCGGAGGCGGGTGCCTTCTAATGTACATGATCCTGCCTGAACCACAAACCAGAGGTCGTTATTGGCTTCCCATTATTGGGGTTGCGCTGGTTTGCCTTCCATGGGTGTTCTGGTTCCTCACATTCGTATACAGACTCATTTCTCGGCGCTCTGGATCTAGGTTTGGCGTTGGCAATGCCGGAGGAGCTGGTGGTGCAAAAAATATTAATACAGCTGCAGCTGCAGGTGCGAGTGTACGGGCCGGCACGGATGCGGCTACACCCGCTGATAAGTCTTCCGGGGGTGAAACCGGTGCGATGGTAGCTGGAGAGATAGAATTAGGAAGTTTCAAGGATGGAAAGCGAAGGGTTTCATCGTTAAGCCGCACTTCGTCAAGCATTAATGTATCTAATCATTCTAATCATTCTCATGAGAGTGAAATGCCTCTGGCCACATCAATGGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

18.86

Weight (kDa)

9.74

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 222
AciI CCGC 4 cut(s) 102, 347, 357, 457
AclWI GGATC 2 cut(s) 118, 256
AcoI YGGCCR 2 cut(s) 93, 522
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 2 cut(s) 119, 330
AfiI CCNNNNNNNGG 4 cut(s) 102, 170, 171, 196
AgeI ACCGGT 1 cut(s) 383
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 4 cut(s) 281, 308, 314, 398
AluI AGCT 4 cut(s) 281, 308, 314, 398
AlwI GGATC 2 cut(s) 118, 256
AoxI GGCC 3 cut(s) 93, 333, 522
ApeKI GCWGC 3 cut(s) 308, 311, 314
ArsI GACNNNNNNTTYG 2 cut(s) 133, 165
AseI ATTAAT 2 cut(s) 300, 474
AsiGI ACCGGT 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 2 cut(s) 181, 243
AspS9I GGNCC 1 cut(s) 333
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 32, 389
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 524
BanI GGYRCC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 295, 301, 323
BccI CCATC 3 cut(s) 95, 385, 422
BciT130I CCWGG 1 cut(s) 42
BciVI GTATCC 1 cut(s) 94
BclI TGATCA 1 cut(s) 22
BcnI CCSGG 1 cut(s) 373
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 309, 315
BfoI RGCGCY 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 5 cut(s) 309, 312, 315, 348, 457
BlsI GCNGC 5 cut(s) 310, 313, 316, 349, 458
Bme1390I CCNGG 2 cut(s) 42, 373
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 2 cut(s) 107, 209
BmrFI CCNGG 2 cut(s) 42, 373
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 373
BsaJI CCNNGG 3 cut(s) 40, 194, 372
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 170, 171, 196
Bse118I RCCGGY 2 cut(s) 335, 383
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 3 cut(s) 40, 194, 372
BseGI GGATG 2 cut(s) 349, 433
BseLI CCNNNNNNNGG 4 cut(s) 102, 170, 171, 196
BseMI GCAATG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 3 cut(s) 295, 301, 323
BshFI GGCC 3 cut(s) 95, 335, 524
BshNI GGYRCC 1 cut(s) 105
BshTI ACCGGT 1 cut(s) 383
BsiSI CCGG 5 cut(s) 96, 273, 336, 372, 384
BslI CCNNNNNNNGG 4 cut(s) 102, 170, 171, 196
BsnI GGCC 3 cut(s) 95, 335, 524
Bsp1286I GDGCHC 1 cut(s) 40
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 3 cut(s) 22, 123, 248
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 4 cut(s) 102, 347, 357, 457
BspANI GGCC 3 cut(s) 95, 335, 524
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 107, 209
BspMAI CTGCAG 2 cut(s) 313, 319
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 118, 256
BspT107I GGYRCC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 274
BsrFI RCCGGY 2 cut(s) 335, 383
BsrGI TGTACA 1 cut(s) 117
BssAI RCCGGY 2 cut(s) 335, 383
BssECI CCNNGG 3 cut(s) 40, 194, 372
BssMI GATC 3 cut(s) 22, 123, 248
BssNAI GTATAC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 194
Bst1107I GTATAC 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 337
BstDSI CCRYGG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 349, 433
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 2 cut(s) 181, 243
BstKTI GATC 3 cut(s) 25, 126, 251
BstMBI GATC 3 cut(s) 22, 123, 248
BstMWI GCNNNNNNNGC 6 cut(s) 278, 287, 314, 320, 356, 395
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 2 cut(s) 40, 371
BstSFI CTRYAG 2 cut(s) 309, 315
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 3 cut(s) 295, 301, 323
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 1 cut(s) 248
BstXI CCANNNNNNTGG 1 cut(s) 532
BstYI RGATCY 1 cut(s) 248
BstZ17I GTATAC 1 cut(s) 223
BsuI GTATCC 1 cut(s) 94
BsuRI GGCC 3 cut(s) 95, 335, 524
BtgI CCRYGG 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 349, 433
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 337
CciI TCATGA 1 cut(s) 502
CfoI GCGC 2 cut(s) 181, 243
Cfr10I RCCGGY 2 cut(s) 335, 383
Cfr13I GGNCC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 118, 329
CspAI ACCGGT 1 cut(s) 383
CviAII CATG 3 cut(s) 121, 195, 503
CviQI GTAC 2 cut(s) 118, 329
DpnI GATC 3 cut(s) 24, 125, 250
DpnII GATC 3 cut(s) 22, 123, 248
EaeI YGGCCR 2 cut(s) 93, 522
Eco130I CCWWGG 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 3 cut(s) 124, 198, 506
FaiI YATR 6 cut(s) 56, 58, 122, 196, 223, 504
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FatI CATG 3 cut(s) 120, 194, 502
FauI CCCGC 2 cut(s) 95, 364
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 22
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 5 cut(s) 309, 312, 315, 348, 457
FokI GGATG 2 cut(s) 356, 440
Fsp4HI GCNGC 5 cut(s) 309, 312, 315, 348, 457
FspBI CTAG 1 cut(s) 252
GlaI GCGC 2 cut(s) 180, 242
GluI GCNGC 5 cut(s) 309, 312, 315, 348, 457
GsuI CTGGAG 1 cut(s) 420
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 3 cut(s) 95, 335, 524
HapII CCGG 5 cut(s) 96, 273, 336, 372, 384
HhaI GCGC 2 cut(s) 181, 243
Hin1II CATG 3 cut(s) 124, 198, 506
Hin6I GCGC 2 cut(s) 179, 241
HinP1I GCGC 2 cut(s) 179, 241
HinfI GANTC 2 cut(s) 31, 228
HpaII CCGG 5 cut(s) 96, 273, 336, 372, 384
HphI GGTGA 2 cut(s) 32, 389
Hpy166II GTNNAC 2 cut(s) 223, 329
Hpy188III TCNNGA 3 cut(s) 246, 503, 540
Hpy8I GTNNAC 2 cut(s) 223, 329
HpyAV CCTTC 4 cut(s) 7, 119, 200, 432
HpyCH4V TGCA 3 cut(s) 290, 311, 317
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 287, 314, 320, 356, 395
Hsp92II CATG 3 cut(s) 124, 198, 506
HspAI GCGC 2 cut(s) 179, 241
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 3 cut(s) 22, 123, 248
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 3 cut(s) 295, 301, 323
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 252
MalI GATC 3 cut(s) 24, 125, 250
MboI GATC 3 cut(s) 22, 123, 248
MboII GAAGA 1 cut(s) 360
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 6 cut(s) 38, 92, 140, 221, 269, 528
Mox20I TGGCCA 1 cut(s) 524
MroNI GCCGGC 1 cut(s) 335
MroXI GAANNNNTTC 1 cut(s) 442
MscI TGGCCA 1 cut(s) 524
MseI TTAA 3 cut(s) 300, 452, 474
MslI CAYNNNNRTG 3 cut(s) 501, 507, 530
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 3 cut(s) 308, 314, 359
MspI CCGG 5 cut(s) 96, 273, 336, 372, 384
MspR9I CCNGG 2 cut(s) 42, 373
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 6 cut(s) 278, 287, 314, 320, 356, 395
NaeI GCCGGC 1 cut(s) 337
NciI CCSGG 1 cut(s) 373
NcoI CCATGG 1 cut(s) 194
NdeI CATATG 1 cut(s) 56
NdeII GATC 3 cut(s) 22, 123, 248
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 3 cut(s) 124, 198, 506
NlaIV GGNNCC 2 cut(s) 107, 209
NmeAIII GCCGAG 1 cut(s) 217
PagI TCATGA 1 cut(s) 502
PaqCI CACCTGC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 1 cut(s) 31
PinAI ACCGGT 1 cut(s) 383
PkrI GCNGC 5 cut(s) 310, 313, 316, 349, 458
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PshBI ATTAAT 2 cut(s) 300, 474
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 107, 209
PspPI GGNCC 1 cut(s) 333
PstI CTGCAG 2 cut(s) 313, 319
PsuI RGATCY 1 cut(s) 248
PvuII CAGCTG 2 cut(s) 308, 314
RsaI GTAC 2 cut(s) 119, 330
RsaNI GTAC 2 cut(s) 118, 329
RseI CAYNNNNRTG 3 cut(s) 501, 507, 530
SaqAI TTAA 3 cut(s) 300, 452, 474
SatI GCNGC 5 cut(s) 309, 312, 315, 348, 457
Sau3AI GATC 3 cut(s) 22, 123, 248
Sau96I GGNCC 1 cut(s) 333
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 2 cut(s) 42, 373
SduI GDGCHC 1 cut(s) 40
SetI ASST 8 cut(s) 49, 151, 257, 283, 310, 316, 322, 400
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 2 cut(s) 309, 315
SmiMI CAYNNNNRTG 3 cut(s) 501, 507, 530
Sse9I AATT 1 cut(s) 410
SsiI CCGC 4 cut(s) 102, 347, 357, 457
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 40, 371
StyI CCWWGG 1 cut(s) 194
TasI AATT 1 cut(s) 410
TatI WGTACW 1 cut(s) 117
TauI GCSGC 2 cut(s) 350, 459
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 3 cut(s) 300, 452, 474
Tru9I TTAA 3 cut(s) 300, 452, 474
TseI GCWGC 3 cut(s) 308, 311, 314
TspDTI ATGAA 5 cut(s) 17, 43, 44, 52, 435
TspGWI ACGGA 1 cut(s) 356
VspI ATTAAT 2 cut(s) 300, 474
XcmI CCANNNNNNNNNTGG 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.