Rorug02G0236800

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005749
Physical Location & Seq
Forward (+)
23669805 .. 23674006
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0236800.1

Sequence Viewer

Length: 1857 bp
ATGAACAAGTCTCTAACTACAACACAAAATCCAGGCAGTCATTACAATCGCAGTGATCAACTTCAAGACCAAAGCATTGTGATTCCCGCGAAACTCATCAATCTCGCTGATAGCTTCGAAAAGAAAGAACACTCCTGGTTCATCACTTCTCATGTCCCAACGGACCTTACAATTCGAGTTCAAGAAATCACCTTCAATGTTCACAAGTATCCTTTGGTAGCAAAATGTGGCTACATAGGTCAATTGGAATTTCAGCCTTCGATCTCAAATTTTGGCAATGACTTGAAGCTTGAAAACTTCCCAGGTGGACCAGAAACCTTTGAAATGATTCTAAAATTCTGTTATGGTCTCCCACTGGATTTAAACCCTAACAATATAGCTCCACTAAGATGCGGATCAGAATTTCTAGAAATGACTGAAGAATTTCAAGCTGGAAATCTGATTTCCAAGACTGAAGCTTTTCTGACATTGGTAGTCCTTTCTTCTTGGAAAGATACCATCACTGTACTTAAGTCCTGTGAAACTCTATCTCCATGGGCTGAGAATCTTCAAATTGTCAGAAGATGCTGCGACTCAATTGCATGGAAGGCTACTAGAGAAAAAACCAGTAGGGATGCCGACGGTCAAGAACAATGGTGGTTTTGTGAAGTGGCCACCTTTCGAATAGATCATTTCATGAGGGTCATATCGACAATAAAAGCAAAGAATACAAGGCCAGAGTTTATAGGTAAATGTATTATGCACTATGCAGAAAGATGGTTGCCAGGCATGGATGTGGAGTTTGAAGGACTAAGAGGATATGGAATTGGAAGAAATGACCTGCAGTTCAGTATCCTGAGTAACAGGAAAGAGGAAGGCGATTATGGACACAGCCAGGAGCAAAAAGCAATTATTGAAAGCCTCCTAAGCGTACTTCCTCCCCAAAATGAAGCTGTTCCATGTAAATTCTTATTGCAGATGTTAAAGATGGCAATGATGTACTCGGCATCGCCAGCTTTGATTTCAGAGCTTGAAAAGAGAGTAGGGATGATGTTAGAAGATGCCACAGTCAATGATTTGCTGATTCCAAGTTATAATATTGCAGACCAAGGGAAATTGGTGACGTCTCCAGAAGAACGCACGATGCACGACGTTGATGTTGTTCAAAGGATTGTGGAATATTTCTTGATGCATGAGCATCAACAGCAAGAGAAGATTGGTAAAACCAATATCAGTAAGCTCTTGGACAGCTACCTGGCTGAGATTGCAGGAGACCCAAATCTCTCGATAACCAAGTTTCAAGTATTGGCTGAATTGCTGCCTGAAAATGCTCGAGCATGTGATGATGGTCTTTATAGGGCTATTGACACCTATCTGAAGACTCATCCTTCACTTCCCGAGCATGATCGAAGAAGGCTATGCAGGATAATGAACTGTGAGAAACTATCACTTGATGCATGCATGCATGCTGCACAAAATGATCGACTGCCTATGAAAACCATTGTCCAGGTTCTGTTCTCAGAGCAAGTAAAGATGAGGACAGCAATGCAAGAGAAGGAATCAGCACCAACTGGCAATAACTCTGAACAGGAAGGAAACCGCTCATCAACAGATGCAGAAATTAAGAATCTTAAAGCGGAACTTGAGAATGTGAAGATAAGGATGGCAGAGCTGCAAAGAGACTACTCAGAGCTGCAACAGGAGAATGAAAAGATGAACAACAAGCAAAAGATTGTGTCAAGTTGGAGTTCAGGATGGAGAAAAATCAAGAAATCTTTCCATACAAAATCTGAAGCCAACGATACTGGAGAAGAAAAACAAAGACCCAACTCAATTGGGGGCAGAATCAGCTCCAGACGAAGGTCATCCATGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

618

Amino Acids

70.57

Weight (kDa)

6.04

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 211 - 480 1.8e-90 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1074
AatII GACGTC 1 cut(s) 1106
Acc36I ACCTGC 1 cut(s) 828
AccBSI CCGCTC 1 cut(s) 1581
AccII CGCG 1 cut(s) 89
AciI CCGC 4 cut(s) 87, 393, 1579, 1616
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 6 cut(s) 248, 268, 335, 401, 422, 944
AcuI CTGAAG 4 cut(s) 438, 474, 1376, 1791
AcyI GRCGYC 1 cut(s) 1103
AfaI GTAC 3 cut(s) 507, 912, 980
AfiI CCNNNNNNNGG 1 cut(s) 1839
AflII CTTAAG 1 cut(s) 509
AjnI CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
Alw26I GTCTC 5 cut(s) 15, 353, 1110, 1245, 1653
AlwI GGATC 1 cut(s) 403
AlwNI CAGNNNCTG 1 cut(s) 1492
Ama87I CYCGRG 2 cut(s) 1311, 1376
AoxI GGCC 2 cut(s) 651, 713
ApeKI GCWGC 5 cut(s) 567, 1297, 1448, 1651, 1672
ApoI RAATTY 6 cut(s) 248, 268, 335, 401, 422, 944
Asp700I GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
AspS9I GGNCC 2 cut(s) 163, 308
AsuHPI GGTGA 2 cut(s) 181, 1111
AsuII TTCGAA 2 cut(s) 117, 661
AvaI CYCGRG 2 cut(s) 1311, 1376
AvaII GGWCC 2 cut(s) 163, 308
BalI TGGCCA 1 cut(s) 653
BbsI GAAGAC 1 cut(s) 1364
BbvI GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BccI CCATC 6 cut(s) 506, 750, 961, 1319, 1636, 1728
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BciVI GTATCC 2 cut(s) 219, 842
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 5 cut(s) 15, 353, 1110, 1245, 1653
BfaI CTAG 2 cut(s) 407, 594
BfmI CTRYAG 1 cut(s) 821
BfrI CTTAAG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 828
BfuI GTATCC 2 cut(s) 219, 842
BisI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
BlsI GCNGC 5 cut(s) 569, 1299, 1450, 1653, 1674
Bme1390I CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
Bme18I GGWCC 2 cut(s) 163, 308
BmeT110I CYCGRG 2 cut(s) 1311, 1376
BmgT120I GGNCC 2 cut(s) 163, 308
BmrFI CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BoxI GACNNNNGTC 1 cut(s) 1840
BpiI GAAGAC 1 cut(s) 1364
BpmI CTGGAG 3 cut(s) 1092, 1806, 1816
Bpu10I CCTNAGC 1 cut(s) 905
Bpu14I TTCGAA 2 cut(s) 117, 661
BpuEI CTTGAG 1 cut(s) 1643
BsaBI GATNNNNATC 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 1103
BsaI GGTCTC 2 cut(s) 353, 1245
BsaJI CCNNGG 3 cut(s) 301, 533, 1087
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 1839
Bse1I ACTGG 4 cut(s) 360, 606, 1555, 1789
Bse3DI GCAATG 3 cut(s) 283, 978, 1530
Bse8I GATNNNNATC 1 cut(s) 394
BseBI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BseDI CCNNGG 3 cut(s) 301, 533, 1087
BseGI GGATG 7 cut(s) 619, 778, 1032, 1363, 1647, 1739, 1844
BseJI GATNNNNATC 1 cut(s) 394
BseLI CCNNNNNNNGG 1 cut(s) 1839
BseMI GCAATG 3 cut(s) 283, 978, 1530
BseMII CTCAG 5 cut(s) 531, 827, 1230, 1512, 1680
BseNI ACTGG 4 cut(s) 360, 606, 1555, 1789
BseXI GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BsgI GTGCAG 1 cut(s) 1434
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 2 cut(s) 653, 715
BsiHKCI CYCGRG 2 cut(s) 1311, 1376
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 1839
BsmAI GTCTC 5 cut(s) 15, 353, 1110, 1245, 1653
BsmBI CGTCTC 1 cut(s) 1110
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 653, 715
Bso31I GGTCTC 2 cut(s) 353, 1245
BsoBI CYCGRG 2 cut(s) 1311, 1376
Bsp119I TTCGAA 2 cut(s) 117, 661
Bsp143I GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 4 cut(s) 87, 393, 1579, 1616
BspANI GGCC 2 cut(s) 653, 715
BspCNI CTCAG 5 cut(s) 532, 828, 1231, 1511, 1679
BspFNI CGCG 1 cut(s) 89
BspHI TCATGA 1 cut(s) 675
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 828
BspPI GGATC 1 cut(s) 403
BspT104I TTCGAA 2 cut(s) 117, 661
BspTI CTTAAG 1 cut(s) 509
BspTNI GGTCTC 2 cut(s) 353, 1245
BsrBI CCGCTC 1 cut(s) 1581
BsrDI GCAATG 3 cut(s) 283, 978, 1530
BsrI ACTGG 4 cut(s) 360, 606, 1555, 1789
BssECI CCNNGG 3 cut(s) 301, 533, 1087
BssMI GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
BssNI GRCGYC 1 cut(s) 1103
BssT1I CCWWGG 2 cut(s) 533, 1087
Bst2UI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
Bst4CI ACNGT 4 cut(s) 505, 623, 1048, 1415
BstACI GRCGYC 1 cut(s) 1103
BstAFI CTTAAG 1 cut(s) 509
BstBI TTCGAA 2 cut(s) 117, 661
BstC8I GCNNGC 4 cut(s) 993, 1438, 1442, 1446
BstDEI CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 7 cut(s) 619, 778, 1032, 1363, 1647, 1739, 1844
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 6 cut(s) 58, 264, 398, 670, 1387, 1462
BstMAI GTCTC 5 cut(s) 15, 353, 1110, 1245, 1653
BstMBI GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
BstMWI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
BstNI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
BstNSI RCATGY 4 cut(s) 1320, 1440, 1444, 1448
BstPAI GACNNNNGTC 1 cut(s) 1840
BstSCI CCNGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
BstSFI CTRYAG 1 cut(s) 821
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
BstV2I GAAGAC 1 cut(s) 1364
BsuI GTATCC 2 cut(s) 219, 842
BsuRI GGCC 2 cut(s) 653, 715
BtgI CCRYGG 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 972
BtsCI GGATG 7 cut(s) 619, 778, 1032, 1363, 1647, 1739, 1844
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 3 cut(s) 58, 353, 501
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 4 cut(s) 993, 1438, 1442, 1446
CaiI CAGNNNCTG 1 cut(s) 1492
CciI TCATGA 1 cut(s) 675
Cfr13I GGNCC 2 cut(s) 163, 308
Csp6I GTAC 3 cut(s) 506, 911, 979
CviQI GTAC 3 cut(s) 506, 911, 979
DdeI CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
DpnI GATC 6 cut(s) 57, 263, 397, 669, 1386, 1461
DpnII GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
DraI TTTAAA 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 651
Eco130I CCWWGG 2 cut(s) 533, 1087
Eco31I GGTCTC 2 cut(s) 353, 1245
Eco47I GGWCC 2 cut(s) 163, 308
Eco57I CTGAAG 4 cut(s) 438, 474, 1376, 1791
Eco88I CYCGRG 2 cut(s) 1311, 1376
EcoRII CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
EcoT14I CCWWGG 2 cut(s) 533, 1087
EcoT22I ATGCAT 4 cut(s) 1173, 1438, 1442, 1446
ErhI CCWWGG 2 cut(s) 533, 1087
Esp3I CGTCTC 1 cut(s) 1110
FalI AAGNNNNNCTT 2 cut(s) 1605, 1637
FaqI GGGAC 1 cut(s) 140
FauI CCCGC 1 cut(s) 94
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
FokI GGATG 7 cut(s) 626, 785, 1039, 1350, 1654, 1746, 1831
Fsp4HI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
FspBI CTAG 2 cut(s) 407, 594
GluI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
GsuI CTGGAG 3 cut(s) 1092, 1806, 1816
HaeIII GGCC 2 cut(s) 653, 715
Hin1I GRCGYC 1 cut(s) 1103
HindIII AAGCTT 2 cut(s) 287, 456
HinfI GANTC 9 cut(s) 82, 328, 544, 572, 1063, 1360, 1538, 1606, 1824
HphI GGTGA 2 cut(s) 181, 1111
Hpy166II GTNNAC 2 cut(s) 202, 308
Hpy8I GTNNAC 2 cut(s) 202, 308
Hpy99I CGWCG 2 cut(s) 623, 1133
HpyCH4III ACNGT 4 cut(s) 505, 623, 1048, 1415
HpyCH4IV ACGT 2 cut(s) 1103, 1131
HpyF10VI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
HpyF3I CTNAG 8 cut(s) 386, 540, 791, 836, 905, 1239, 1498, 1666
HpySE526I ACGT 2 cut(s) 1103, 1131
Hsp92I GRCGYC 1 cut(s) 1103
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
LmnI GCTCC 3 cut(s) 385, 877, 1835
Lsp1109I GCAGC 5 cut(s) 554, 1284, 1435, 1638, 1659
MaeI CTAG 2 cut(s) 407, 594
MaeII ACGT 2 cut(s) 1103, 1131
MaeIII GTNAC 2 cut(s) 839, 1099
MalI GATC 6 cut(s) 57, 263, 397, 669, 1386, 1461
MbiI CCGCTC 1 cut(s) 1581
MboI GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
MfeI CAATTG 3 cut(s) 242, 576, 1812
MlsI TGGCCA 1 cut(s) 653
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 2 cut(s) 566, 1354
MmeI TCCRAC 1 cut(s) 1703
MnlI CCTC 6 cut(s) 672, 788, 844, 911, 927, 1509
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 4 cut(s) 1173, 1438, 1442, 1446
MroXI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
MscI TGGCCA 1 cut(s) 653
MseI TTAA 5 cut(s) 362, 510, 962, 1602, 1611
MslI CAYNNNNRTG 2 cut(s) 388, 773
Msp20I TGGCCA 1 cut(s) 653
MspCI CTTAAG 1 cut(s) 509
MspR9I CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
MunI CAATTG 3 cut(s) 242, 576, 1812
MvaI CCWGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 6 cut(s) 587, 906, 992, 1183, 1244, 1827
NcoI CCATGG 1 cut(s) 533
NdeII GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
NmeAIII GCCGAG 1 cut(s) 962
NmuCI GTSAC 1 cut(s) 1099
NsiI ATGCAT 4 cut(s) 1173, 1438, 1442, 1446
NspI RCATGY 4 cut(s) 1320, 1440, 1444, 1448
NspV TTCGAA 2 cut(s) 117, 661
PaeI GCATGC 3 cut(s) 1440, 1444, 1448
PaeR7I CTCGAG 1 cut(s) 1311
PagI TCATGA 1 cut(s) 675
PdmI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
PfeI GAWTC 7 cut(s) 82, 328, 544, 1063, 1538, 1606, 1824
PkrI GCNGC 5 cut(s) 569, 1299, 1450, 1653, 1674
PleI GAGTC 2 cut(s) 566, 1354
PpsI GAGTC 2 cut(s) 566, 1354
PshAI GACNNNNGTC 1 cut(s) 1840
PsiI TTATAA 1 cut(s) 1074
Psp6I CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
PspGI CCWGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
PspPI GGNCC 2 cut(s) 163, 308
PspXI VCTCGAGB 1 cut(s) 1311
PstI CTGCAG 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 1492
RsaI GTAC 3 cut(s) 507, 912, 980
RsaNI GTAC 3 cut(s) 506, 911, 979
RseI CAYNNNNRTG 2 cut(s) 388, 773
SaqAI TTAA 5 cut(s) 362, 510, 962, 1602, 1611
SatI GCNGC 5 cut(s) 568, 1298, 1449, 1652, 1673
Sau3AI GATC 6 cut(s) 55, 261, 395, 667, 1384, 1459
Sau96I GGNCC 2 cut(s) 163, 308
SchI GAGTC 2 cut(s) 566, 1354
ScrFI CCNGG 7 cut(s) 33, 136, 303, 765, 875, 1235, 1487
SfcI CTRYAG 1 cut(s) 821
Sfr274I CTCGAG 1 cut(s) 1311
SfuI TTCGAA 2 cut(s) 117, 661
SinI GGWCC 2 cut(s) 163, 308
SlaI CTCGAG 1 cut(s) 1311
SmiMI CAYNNNNRTG 2 cut(s) 388, 773
SmlI CTYRAG 3 cut(s) 509, 1311, 1622
SmoI CTYRAG 3 cut(s) 509, 1311, 1622
SphI GCATGC 3 cut(s) 1440, 1444, 1448
SsiI CCGC 4 cut(s) 87, 393, 1579, 1616
SspI AATATT 2 cut(s) 1078, 1160
SspMI CTAG 2 cut(s) 407, 594
StyD4I CCNGG 7 cut(s) 31, 134, 301, 763, 873, 1233, 1485
StyI CCWWGG 2 cut(s) 533, 1087
TaaI ACNGT 4 cut(s) 505, 623, 1048, 1415
TaiI ACGT 2 cut(s) 1106, 1134
TaqI TCGA 9 cut(s) 117, 175, 260, 661, 689, 1265, 1312, 1387, 1462
TatI WGTACW 2 cut(s) 505, 978
TfiI GAWTC 7 cut(s) 82, 328, 544, 1063, 1538, 1606, 1824
Tru1I TTAA 5 cut(s) 362, 510, 962, 1602, 1611
Tru9I TTAA 5 cut(s) 362, 510, 962, 1602, 1611
TscAI CASTG 3 cut(s) 58, 360, 508
TseFI GTSAC 1 cut(s) 1099
TseI GCWGC 5 cut(s) 567, 1297, 1448, 1651, 1672
Tsp45I GTSAC 1 cut(s) 1099
TspDTI ATGAA 8 cut(s) 17, 130, 664, 942, 1424, 1487, 1701, 1709
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 3 cut(s) 58, 360, 508
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 2 cut(s) 163, 308
XapI RAATTY 6 cut(s) 248, 268, 335, 401, 422, 944
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 4 cut(s) 1320, 1440, 1444, 1448
XhoI CTCGAG 1 cut(s) 1311
XmnI GAANNNNTTC 5 cut(s) 327, 423, 459, 933, 1754
XspI CTAG 2 cut(s) 407, 594
ZraI GACGTC 1 cut(s) 1104
Zsp2I ATGCAT 4 cut(s) 1173, 1438, 1442, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.