MD04G1048700.v1.1

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005749
Physical Location & Seq
Reverse (-)
5706189 .. 5709747
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1048700.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGAAATGGCCGTTAATTTCAGCCTCCCCTTCTCGAGTTTAAATTCTAAGGTAATTAGGCACGATCAGAAAATTGGTGGCTCTTTTCCAAGCCTTACTTGCACAAGTGCAAAATCCTCCAATCACATCACATATGCTGCTCAATCAATTTCCAGAAGGAAAAGTCATAAGTATTTTCCTCCAATTTGTGCTGTATTATCAGGCACAGAAGACATTGGAGTATCTTCATCTCAGATTGACGACTTGCCTGTTACTGTTACTACTTCAGGTGCCAATGATGCTGGTGAACTGAAGATAAACGTAGAGGCATCTGGTGCTAAAACTCAAGCAATTTTTGACGAAGTTTTTAATAAAATGGTTACTGCAGCCCAACCAATACCTGGATTTCGAAGAGTGAAGGGAGGCAAGACACCCAATATACCTAGAGACATTTTACTAGAAGTCCTTGGACCTTCGAAAGTTTATAAGCAAGTAATCAAGAAAGTGATCAATTCGGCGGTAGCTGAATATGTAGAGAAGGAAGGTTTAGAGGTTAGAAAGGACTTGAGAGTGGAGCAAAGCTTTGAAGATCTTGAAGATGCTTTCGAAGCAGGTGAAAACTTCCGCTTTGATGCCGTCATTTATCTTCGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

212

Amino Acids

23.14

Weight (kDa)

7.73

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 93 - 207 8.4e-11 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AarI CACCTGC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 380
AciI CCGC 2 cut(s) 497, 604
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 249, 311
AfiI CCNNNNNNNGG 1 cut(s) 380
AgsI TTSAA 2 cut(s) 566, 575
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 2 cut(s) 503, 561
AluI AGCT 2 cut(s) 503, 561
Alw26I GTCTC 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 137, 365
ApoI RAATTY 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 296, 605
AsuII TTCGAA 3 cut(s) 388, 455, 585
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 124, 377
BceAI ACGGC 1 cut(s) 599
BciT130I CCWGG 1 cut(s) 381
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 2 cut(s) 423, 437
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 581
BglII AGATCT 1 cut(s) 568
BisI GCNGC 2 cut(s) 138, 366
BlsI GCNGC 2 cut(s) 139, 367
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 4 cut(s) 268, 317, 568, 601
BpiI GAAGAC 1 cut(s) 216
Bpu14I TTCGAA 3 cut(s) 388, 455, 585
BpuEI CTTGAG 2 cut(s) 309, 565
BsaJI CCNNGG 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMII CTCAG 1 cut(s) 245
BseXI GCAGC 2 cut(s) 124, 377
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 420
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 34
Bsp119I TTCGAA 3 cut(s) 388, 455, 585
Bsp143I GATC 3 cut(s) 64, 486, 568
BspACI CCGC 2 cut(s) 497, 604
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 581
BspT104I TTCGAA 3 cut(s) 388, 455, 585
BspT107I GGYRCC 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 3 cut(s) 64, 486, 568
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 385
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 3 cut(s) 388, 455, 585
BstDEI CTNAG 2 cut(s) 48, 231
BstKTI GATC 3 cut(s) 67, 489, 571
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 3 cut(s) 64, 486, 568
BstMWI GCNNNNNNNGC 4 cut(s) 99, 278, 314, 587
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 124, 377
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 1 cut(s) 11
BveI ACCTGC 1 cut(s) 581
Cfr13I GGNCC 1 cut(s) 449
CviJI RGCY 7 cut(s) 11, 24, 81, 93, 368, 503, 561
CviKI_1 RGCY 7 cut(s) 11, 24, 81, 93, 368, 503, 561
DdeI CTNAG 2 cut(s) 48, 231
DpnI GATC 3 cut(s) 66, 488, 570
DpnII GATC 3 cut(s) 64, 486, 568
DraI TTTAAA 1 cut(s) 42
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 2 cut(s) 249, 311
Eco88I CYCGRG 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 445
ErhI CCWWGG 1 cut(s) 445
FaiI YATR 6 cut(s) 133, 135, 168, 419, 465, 510
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FauNDI CATATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 138, 366
Fsp4HI GCNGC 2 cut(s) 138, 366
FspBI CTAG 2 cut(s) 423, 437
GluI GCNGC 2 cut(s) 138, 366
HaeIII GGCC 1 cut(s) 11
HindIII AAGCTT 1 cut(s) 559
HphI GGTGA 2 cut(s) 296, 605
Hpy166II GTNNAC 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 69, 234
Hpy188III TCNNGA 4 cut(s) 34, 153, 478, 572
Hpy8I GTNNAC 1 cut(s) 287
HpyAV CCTTC 6 cut(s) 40, 150, 391, 462, 511, 515
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 102, 110, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 278, 314, 587
HpyF3I CTNAG 2 cut(s) 48, 231
HpySE526I ACGT 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 3 cut(s) 64, 486, 568
LmnI GCTCC 1 cut(s) 553
LpnPI CCDG 9 cut(s) 166, 186, 252, 261, 267, 297, 366, 393, 576
Lsp1109I GCAGC 2 cut(s) 124, 377
LweI GCATC 4 cut(s) 268, 317, 568, 601
MaeI CTAG 2 cut(s) 423, 437
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 250, 256, 358
MalI GATC 3 cut(s) 66, 488, 570
MboI GATC 3 cut(s) 64, 486, 568
MboII GAAGA 7 cut(s) 216, 221, 304, 402, 578, 587, 617
MflI RGATCY 1 cut(s) 568
MluCI AATT 8 cut(s) 16, 43, 54, 72, 147, 183, 330, 490
MnlI CCTC 6 cut(s) 35, 127, 189, 298, 395, 523
MseI TTAA 3 cut(s) 15, 41, 348
MspR9I CCNGG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 4 cut(s) 99, 278, 314, 587
NdeI CATATG 1 cut(s) 133
NdeII GATC 3 cut(s) 64, 486, 568
NlaIV GGNNCC 1 cut(s) 271
NspV TTCGAA 3 cut(s) 388, 455, 585
PaeR7I CTCGAG 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 581
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 2 cut(s) 139, 367
PsiI TTATAA 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 568
SaqAI TTAA 3 cut(s) 15, 41, 348
SatI GCNGC 2 cut(s) 138, 366
Sau3AI GATC 3 cut(s) 64, 486, 568
Sau96I GGNCC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 381
SfaNI GCATC 4 cut(s) 268, 317, 568, 601
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 34
SfuI TTCGAA 3 cut(s) 388, 455, 585
SinI GGWCC 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 34
SmlI CTYRAG 3 cut(s) 34, 324, 544
SmoI CTYRAG 3 cut(s) 34, 324, 544
Sse9I AATT 8 cut(s) 16, 43, 54, 72, 147, 183, 330, 490
SsiI CCGC 2 cut(s) 497, 604
SspMI CTAG 2 cut(s) 423, 437
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 1 cut(s) 303
TaqI TCGA 4 cut(s) 35, 388, 455, 585
TasI AATT 8 cut(s) 16, 43, 54, 72, 147, 183, 330, 490
Tru1I TTAA 3 cut(s) 15, 41, 348
Tru9I TTAA 3 cut(s) 15, 41, 348
TseI GCWGC 2 cut(s) 137, 365
TspDTI ATGAA 1 cut(s) 216
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 34
XspI CTAG 2 cut(s) 423, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.