Rw5G027570

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005749
Physical Location & Seq
Reverse (-)
40877521 .. 40882054
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027570.1

Sequence Viewer

Length: 627 bp
ATGGCCACGCTGACGACGACAGTTTCTTCACAGTTCCCCTCCCTTCAATTACCCAAGCAGTTTTCTGTAAGAAGCTACTGCACTTACGTCCTTGCTTGTCAAAATGCTACAAGACTTGGGTTCTCTACCACACCCCATCATCTTTTTACTGGGAGAAGTCTCAAGTATCTTCCTGCTATCTGTGCTGTATTGTCAGGCACAGAAGACATTGGAGTATCCTCTACTCAATCTGAAGACTTGTCTATTACTTCTACTACTTCAGGTACCGATGCTGGAGAATTTAAGATAAGCGTAGAGGTATCTGGGGCCAAAACTCAAGCAATATTCGATGACGTTTTTGACAAAATGGTTGCTGCAGCCCAGCCAATACCTGGATTTCGAAGAGTGAAAGGAGGGAAAACACCGAATATACCTAGAGATGTTTTGGTCGAAGTCCTTGGACCGTCGAAAGTATATAAGCAAGTAATCAAGAAAGTGATCAACTCTACAGTAGCTGAATATGTAGAGAAGGAATGTTTAAAAGTCAGCAAGGACTTGAGAGTGGAGCAAAGCTTTGAAGATCTTGAAGCCTCTTTTGAACCCGGTGAAGAATTCAGCTTTGATGCTGTAGTTCAACTTCTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

208

Amino Acids

22.66

Weight (kDa)

5.77

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 206 3.2e-11 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 278, 590
AcuI CTGAAG 2 cut(s) 243, 252
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 5 cut(s) 47, 557, 566, 578, 614
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 4 cut(s) 75, 494, 552, 597
AluI AGCT 4 cut(s) 75, 494, 552, 597
Alw26I GTCTC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 2 cut(s) 3, 306
ApeKI GCWGC 2 cut(s) 353, 356
ApoI RAATTY 2 cut(s) 278, 590
Asp718I GGTACC 1 cut(s) 263
AspS9I GGNCC 2 cut(s) 306, 440
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 1 cut(s) 596
AsuII TTCGAA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 263
BarI GAAGNNNNNNTAC 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 210, 240
BbvI GCAGC 2 cut(s) 340, 368
BccI CCATC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 372
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 1 cut(s) 477
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 3 cut(s) 354, 486, 606
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 1 cut(s) 559
BisI GCNGC 2 cut(s) 354, 357
BlsI GCNGC 2 cut(s) 355, 358
Bme1390I CCNGG 2 cut(s) 372, 582
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 2 cut(s) 306, 440
BmiI GGNNCC 2 cut(s) 265, 307
BmrFI CCNGG 2 cut(s) 372, 582
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 259, 592
BmuI ACTGGG 1 cut(s) 159
BpiI GAAGAC 2 cut(s) 210, 240
BpmI CTGGAG 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 379
BpuEI CTTGAG 3 cut(s) 146, 300, 556
BpuMI CCSGG 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 2 cut(s) 340, 368
BseYI CCCAGC 1 cut(s) 360
BsgI GTGCAG 1 cut(s) 64
BshFI GGCC 2 cut(s) 5, 308
BshNI GGYRCC 1 cut(s) 263
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 2 cut(s) 5, 308
Bsp119I TTCGAA 1 cut(s) 379
Bsp143I GATC 2 cut(s) 477, 559
BspANI GGCC 2 cut(s) 5, 308
BspLI GGNNCC 2 cut(s) 265, 307
BspMAI CTGCAG 1 cut(s) 358
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 2 cut(s) 477, 559
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 4 cut(s) 22, 33, 444, 490
Bst6I CTCTTC 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 379
BstKTI GATC 2 cut(s) 480, 562
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 477, 559
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 370, 580
BstSFI CTRYAG 3 cut(s) 354, 486, 606
BstV1I GCAGC 2 cut(s) 340, 368
BstV2I GAAGAC 2 cut(s) 210, 240
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 2 cut(s) 5, 308
CaiI CAGNNNCTG 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 306, 440
Csp6I GTAC 1 cut(s) 264
CviJI RGCY 9 cut(s) 5, 75, 308, 359, 364, 494, 552, 569, 597
CviKI_1 RGCY 9 cut(s) 5, 75, 308, 359, 364, 494, 552, 569, 597
CviQI GTAC 1 cut(s) 264
DpnI GATC 2 cut(s) 479, 561
DpnII GATC 2 cut(s) 477, 559
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 2 cut(s) 243, 252
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
FaiI YATR 4 cut(s) 410, 454, 456, 501
FbaI TGATCA 1 cut(s) 477
Fnu4HI GCNGC 2 cut(s) 354, 357
Fsp4HI GCNGC 2 cut(s) 354, 357
FspBI CTAG 1 cut(s) 414
GluI GCNGC 2 cut(s) 354, 357
GsaI CCCAGC 1 cut(s) 364
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 5, 308
HapII CCGG 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 550
HpaII CCGG 1 cut(s) 582
HphI GGTGA 1 cut(s) 596
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 2 cut(s) 469, 563
Hpy99I CGWCG 2 cut(s) 19, 448
HpyAV CCTTC 2 cut(s) 53, 502
HpyCH4III ACNGT 4 cut(s) 22, 33, 444, 490
HpyCH4IV ACGT 2 cut(s) 87, 333
HpyCH4V TGCA 2 cut(s) 81, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpySE526I ACGT 2 cut(s) 87, 333
KpnI GGTACC 1 cut(s) 267
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 2 cut(s) 477, 559
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 2 cut(s) 340, 368
LweI GCATC 2 cut(s) 259, 592
MaeI CTAG 1 cut(s) 414
MaeII ACGT 2 cut(s) 87, 333
MalI GATC 2 cut(s) 479, 561
MboI GATC 2 cut(s) 477, 559
MboII GAAGA 7 cut(s) 18, 161, 215, 245, 393, 569, 599
MflI RGATCY 1 cut(s) 559
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 47, 278, 590
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 49, 229, 289, 386, 580
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 282, 518
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 2 cut(s) 372, 582
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 1 cut(s) 582
NdeII GATC 2 cut(s) 477, 559
NlaIV GGNNCC 2 cut(s) 265, 307
NspV TTCGAA 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 2 cut(s) 355, 358
Psp6I CCWGG 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 360
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 265, 307
PspPI GGNCC 2 cut(s) 306, 440
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 282, 518
SatI GCNGC 2 cut(s) 354, 357
Sau3AI GATC 2 cut(s) 477, 559
Sau96I GGNCC 2 cut(s) 306, 440
ScrFI CCNGG 2 cut(s) 372, 582
SfaNI GCATC 2 cut(s) 259, 592
SfcI CTRYAG 3 cut(s) 354, 486, 606
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 3 cut(s) 161, 315, 535
SmoI CTYRAG 3 cut(s) 161, 315, 535
Sse9I AATT 3 cut(s) 47, 278, 590
SspI AATATT 1 cut(s) 324
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 370, 580
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 4 cut(s) 22, 33, 444, 490
TaiI ACGT 2 cut(s) 90, 336
TaqI TCGA 4 cut(s) 327, 379, 429, 446
TasI AATT 3 cut(s) 47, 278, 590
Tru1I TTAA 2 cut(s) 282, 518
Tru9I TTAA 2 cut(s) 282, 518
TseI GCWGC 2 cut(s) 353, 356
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 2 cut(s) 278, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.