Rroxscaffold_1G00036610

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005749
Physical Location & Seq
Forward (+)
53455784 .. 53460002
4219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036610.1

Sequence Viewer

Length: 516 bp
ATGGCCACACTGACGACGACAGTTTCTTCACAGTTCCCCTCCCTGCAATTACCCAAGGTATTTCCTGCAGCAGTTTTCTGTAAGAAGCTACTGCACTTACGTCCTTGCTTGTCAAAATGCTACAAGACTTGGGTTCTCTACCACACCCCATCATCTTTTTACTGGATAAGCGTAGAGGTATCTGGGGCCAAAACTCAAGCAATATTCGATGACGTTTTTGACAAAATGGTTGCTGCAGCCCAGCCAATACCCGGATTTCGAAGAGTGAAAGGAGGGAAAACACCAAATATACCTAGAGATGTTTTGGTCGAAGTCCTTGGACCGTCGAAAGTATATAAGCAAGTAATCAAGAAAGTGATCAACTCTACAGTAGCTGAATATGTAGAGAAGGTAGTTGTTGGAGTGTTTAAAAGTCAGCAAGGACTTGAGAGTGGAGCAAAGCTTGAAGATCTTGAAGCCTCTTTTGAACCCGGCGAAGAATTCAGCTTTGATGCTGTAGTTCATCTTCTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

171

Amino Acids

18.94

Weight (kDa)

9.08

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 56 - 169 1.7e-08 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 3 cut(s) 446, 455, 467
AluBI AGCT 4 cut(s) 88, 374, 442, 486
AluI AGCT 4 cut(s) 88, 374, 442, 486
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 2 cut(s) 3, 186
ApeKI GCWGC 3 cut(s) 68, 233, 236
ApoI RAATTY 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 186, 320
AsuC2I CCSGG 2 cut(s) 252, 471
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 80, 220, 248
BccI CCATC 1 cut(s) 157
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 2 cut(s) 252, 471
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 4 cut(s) 66, 234, 366, 495
BglII AGATCT 1 cut(s) 448
BisI GCNGC 3 cut(s) 69, 234, 237
BlsI GCNGC 3 cut(s) 70, 235, 238
Bme1390I CCNGG 2 cut(s) 252, 471
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 186, 320
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 2 cut(s) 252, 471
BmsI GCATC 1 cut(s) 481
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 2 cut(s) 180, 446
BpuMI CCSGG 2 cut(s) 252, 471
BsaJI CCNNGG 2 cut(s) 54, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 54, 316
BseLI CCNNNNNNNGG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 3 cut(s) 80, 220, 248
BseYI CCCAGC 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 2 cut(s) 5, 188
BsiSI CCGG 2 cut(s) 252, 471
BslI CCNNNNNNNGG 1 cut(s) 251
BsnI GGCC 2 cut(s) 5, 188
Bsp119I TTCGAA 1 cut(s) 259
Bsp143I GATC 2 cut(s) 357, 448
BspANI GGCC 2 cut(s) 5, 188
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 2 cut(s) 70, 238
BspT104I TTCGAA 1 cut(s) 259
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 54, 316
BssMI GATC 2 cut(s) 357, 448
BssT1I CCWWGG 2 cut(s) 54, 316
Bst4CI ACNGT 4 cut(s) 22, 33, 324, 370
Bst6I CTCTTC 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 259
BstKTI GATC 2 cut(s) 360, 451
BstMBI GATC 2 cut(s) 357, 448
BstSCI CCNGG 2 cut(s) 250, 469
BstSFI CTRYAG 4 cut(s) 66, 234, 366, 495
BstV1I GCAGC 3 cut(s) 80, 220, 248
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 2 cut(s) 5, 188
BtsIMutI CAGTG 1 cut(s) 8
CaiI CAGNNNCTG 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 186, 320
CviJI RGCY 9 cut(s) 5, 88, 188, 239, 244, 374, 442, 458, 486
CviKI_1 RGCY 9 cut(s) 5, 88, 188, 239, 244, 374, 442, 458, 486
DpnI GATC 2 cut(s) 359, 450
DpnII GATC 2 cut(s) 357, 448
DraI TTTAAA 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 54, 316
Eco47I GGWCC 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 479
EcoT14I CCWWGG 2 cut(s) 54, 316
ErhI CCWWGG 2 cut(s) 54, 316
FaiI YATR 4 cut(s) 290, 334, 336, 381
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 3 cut(s) 69, 234, 237
Fsp4HI GCNGC 3 cut(s) 69, 234, 237
FspBI CTAG 1 cut(s) 294
GluI GCNGC 3 cut(s) 69, 234, 237
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 2 cut(s) 5, 188
HapII CCGG 2 cut(s) 252, 471
HindIII AAGCTT 1 cut(s) 440
HpaII CCGG 2 cut(s) 252, 471
Hpy188III TCNNGA 2 cut(s) 349, 452
Hpy99I CGWCG 2 cut(s) 19, 328
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 4 cut(s) 22, 33, 324, 370
HpyCH4IV ACGT 2 cut(s) 100, 213
HpyCH4V TGCA 4 cut(s) 46, 68, 94, 236
HpySE526I ACGT 2 cut(s) 100, 213
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 2 cut(s) 357, 448
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 7 cut(s) 56, 78, 148, 168, 254, 265, 484
Lsp1109I GCAGC 3 cut(s) 80, 220, 248
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 294
MaeII ACGT 2 cut(s) 100, 213
MalI GATC 2 cut(s) 359, 450
MboI GATC 2 cut(s) 357, 448
MboII GAAGA 5 cut(s) 18, 273, 458, 488, 497
MflI RGATCY 1 cut(s) 448
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 47, 479
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 4 cut(s) 49, 169, 266, 469
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 252, 471
MspR9I CCNGG 2 cut(s) 252, 471
NciI CCSGG 2 cut(s) 252, 471
NdeII GATC 2 cut(s) 357, 448
NlaIV GGNNCC 1 cut(s) 187
NspV TTCGAA 1 cut(s) 259
PkrI GCNGC 3 cut(s) 70, 235, 238
PspFI CCCAGC 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 186, 320
PstI CTGCAG 2 cut(s) 70, 238
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 448
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 3 cut(s) 69, 234, 237
Sau3AI GATC 2 cut(s) 357, 448
Sau96I GGNCC 2 cut(s) 186, 320
ScrFI CCNGG 2 cut(s) 252, 471
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 4 cut(s) 66, 234, 366, 495
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 195, 425
SmoI CTYRAG 2 cut(s) 195, 425
Sse9I AATT 2 cut(s) 47, 479
SspI AATATT 1 cut(s) 204
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 250, 469
StyI CCWWGG 2 cut(s) 54, 316
TaaI ACNGT 4 cut(s) 22, 33, 324, 370
TaiI ACGT 2 cut(s) 103, 216
TaqI TCGA 4 cut(s) 207, 259, 309, 326
TasI AATT 2 cut(s) 47, 479
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 15
TseI GCWGC 3 cut(s) 68, 233, 236
TspDTI ATGAA 1 cut(s) 491
TspRI CASTG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 320
XapI RAATTY 1 cut(s) 479
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.