RchiOBHm_Chr5g0044351

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005749
Physical Location & Seq
Reverse (-)
39675335 .. 39679809
4475 bp
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UTR
Exon/CDS
Intron
PRQ32256

Sequence Viewer

Length: 624 bp
ATGGCCACACTGACGACGACAGTTTCTTCACAGTTCCCCTCCCTTCAATTACCCAAGTTTTCTGTAAGAAGCTACTGCACTTACGTCCTTGCTTGTCAAAATGCTACAAGACTAGGGTTCTCTACCACACCCCATCATCTGTTTACTGGGAGAAGTCTCAAGTATCTTCCTGCTATCTGTGCTGTATTGTCAGGCACAGAAGACATTGGAGTATCCTCTACTCAATCTGAAGACTTGTCTATTACCTCTACTACTTCAGGTACCAATGCTGGAGAATTTAAGATAAGCGTAGAGGTATCTGGGGCCAAAACTCAAGCAATATTCGATGACGTTTTTGACAAAATGGTTGCTGCAGCCCAGCCAATACCTGGATTTCGAAGAGTGAAAGGAGGGAAAACACCGAATATACCTAGAGATGTTTTGGTCGAAGTCCTTGGACCGTCGAAAGTATATAAGCAAGTAATCAAGAAAGTGATCAACTCTACAGTAGCTGAATATGTAGAGAAGGAGTGTTTAAAAGTCAGCAAGGACTTGAGAGTGGAGCAAAGCTTTGAAGATCTTGAAGCCTCTTTTGAACCCGGCGAAGAATTCAGCTTTGATGCTGTAGTTCAACTTCTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

207

Amino Acids

22.53

Weight (kDa)

6.16

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 205 3.1e-11 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 275, 587
AcuI CTGAAG 2 cut(s) 240, 249
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 1 cut(s) 368
AgsI TTSAA 5 cut(s) 47, 554, 563, 575, 611
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 4 cut(s) 72, 491, 549, 594
AluI AGCT 4 cut(s) 72, 491, 549, 594
Alw26I GTCTC 1 cut(s) 161
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 2 cut(s) 3, 303
ApeKI GCWGC 2 cut(s) 350, 353
ApoI RAATTY 2 cut(s) 275, 587
Asp718I GGTACC 1 cut(s) 260
AspS9I GGNCC 2 cut(s) 303, 437
AsuC2I CCSGG 1 cut(s) 579
AsuII TTCGAA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 437
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 260
BarI GAAGNNNNNNTAC 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 207, 237
BbvI GCAGC 2 cut(s) 337, 365
BccI CCATC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 369
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 113, 411
BfmI CTRYAG 3 cut(s) 351, 483, 603
BfuI GTATCC 1 cut(s) 223
BglII AGATCT 1 cut(s) 556
BisI GCNGC 2 cut(s) 351, 354
BlsI GCNGC 2 cut(s) 352, 355
Bme1390I CCNGG 2 cut(s) 369, 579
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 303, 437
BmiI GGNNCC 2 cut(s) 262, 304
BmrFI CCNGG 2 cut(s) 369, 579
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 1 cut(s) 589
BmuI ACTGGG 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 207, 237
BpmI CTGGAG 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 3 cut(s) 143, 297, 553
BpuMI CCSGG 1 cut(s) 579
BsaJI CCNNGG 1 cut(s) 433
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 337, 365
BseYI CCCAGC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 61
BshFI GGCC 2 cut(s) 5, 305
BshNI GGYRCC 1 cut(s) 260
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 2 cut(s) 5, 305
Bsp119I TTCGAA 1 cut(s) 376
Bsp143I GATC 2 cut(s) 474, 556
BspANI GGCC 2 cut(s) 5, 305
BspLI GGNNCC 2 cut(s) 262, 304
BspMAI CTGCAG 1 cut(s) 355
BspT104I TTCGAA 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 474, 556
BssT1I CCWWGG 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 4 cut(s) 22, 33, 441, 487
Bst6I CTCTTC 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 376
BstKTI GATC 2 cut(s) 477, 559
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 474, 556
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 367, 577
BstSFI CTRYAG 3 cut(s) 351, 483, 603
BstV1I GCAGC 2 cut(s) 337, 365
BstV2I GAAGAC 2 cut(s) 207, 237
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 2 cut(s) 5, 305
BtsIMutI CAGTG 1 cut(s) 8
CaiI CAGNNNCTG 1 cut(s) 491
Cfr13I GGNCC 2 cut(s) 303, 437
Csp6I GTAC 1 cut(s) 261
CviJI RGCY 9 cut(s) 5, 72, 305, 356, 361, 491, 549, 566, 594
CviKI_1 RGCY 9 cut(s) 5, 72, 305, 356, 361, 491, 549, 566, 594
CviQI GTAC 1 cut(s) 261
DpnI GATC 2 cut(s) 476, 558
DpnII GATC 2 cut(s) 474, 556
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 2 cut(s) 240, 249
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
FaiI YATR 4 cut(s) 407, 451, 453, 498
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 2 cut(s) 351, 354
Fsp4HI GCNGC 2 cut(s) 351, 354
FspBI CTAG 2 cut(s) 113, 411
GluI GCNGC 2 cut(s) 351, 354
GsaI CCCAGC 1 cut(s) 361
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 5, 305
HapII CCGG 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 547
HpaII CCGG 1 cut(s) 579
Hpy166II GTNNAC 1 cut(s) 144
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 2 cut(s) 466, 560
Hpy8I GTNNAC 1 cut(s) 144
Hpy99I CGWCG 2 cut(s) 19, 445
HpyAV CCTTC 2 cut(s) 53, 499
HpyCH4III ACNGT 4 cut(s) 22, 33, 441, 487
HpyCH4IV ACGT 2 cut(s) 84, 330
HpyCH4V TGCA 2 cut(s) 78, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpySE526I ACGT 2 cut(s) 84, 330
KpnI GGTACC 1 cut(s) 264
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 2 cut(s) 474, 556
LmnI GCTCC 1 cut(s) 541
Lsp1109I GCAGC 2 cut(s) 337, 365
LweI GCATC 1 cut(s) 589
MaeI CTAG 2 cut(s) 113, 411
MaeII ACGT 2 cut(s) 84, 330
MalI GATC 2 cut(s) 476, 558
MboI GATC 2 cut(s) 474, 556
MboII GAAGA 7 cut(s) 18, 158, 212, 242, 390, 566, 596
MflI RGATCY 1 cut(s) 556
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 47, 275, 587
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 49, 226, 256, 286, 383, 577
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 279, 515
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 2 cut(s) 369, 579
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 579
NdeII GATC 2 cut(s) 474, 556
NlaIV GGNNCC 2 cut(s) 262, 304
NspV TTCGAA 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 368
PkrI GCNGC 2 cut(s) 352, 355
Psp6I CCWGG 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 357
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 2 cut(s) 262, 304
PspPI GGNCC 2 cut(s) 303, 437
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 279, 515
SatI GCNGC 2 cut(s) 351, 354
Sau3AI GATC 2 cut(s) 474, 556
Sau96I GGNCC 2 cut(s) 303, 437
ScrFI CCNGG 2 cut(s) 369, 579
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 3 cut(s) 351, 483, 603
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 1 cut(s) 437
SmlI CTYRAG 3 cut(s) 158, 312, 532
SmoI CTYRAG 3 cut(s) 158, 312, 532
Sse9I AATT 3 cut(s) 47, 275, 587
SspI AATATT 1 cut(s) 321
SspMI CTAG 2 cut(s) 113, 411
StyD4I CCNGG 2 cut(s) 367, 577
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 4 cut(s) 22, 33, 441, 487
TaiI ACGT 2 cut(s) 87, 333
TaqI TCGA 4 cut(s) 324, 376, 426, 443
TasI AATT 3 cut(s) 47, 275, 587
Tru1I TTAA 2 cut(s) 279, 515
Tru9I TTAA 2 cut(s) 279, 515
TscAI CASTG 1 cut(s) 15
TseI GCWGC 2 cut(s) 350, 353
TspRI CASTG 1 cut(s) 15
Van91I CCANNNNNTGG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 2 cut(s) 275, 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XspI CTAG 2 cut(s) 113, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.