pycom01g00490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008605
Physical Location & Seq
Forward (+)
560387 .. 561225
839 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00490.1

Sequence Viewer

Length: 753 bp
ATGGCAAATATAGTAGTATTATTTATAGGTGATAAAAAACTGAACCCGCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGTGTACGGGCGGGTTTGTTTTGGCATGGCCTCAATGCAACAACATTCGCAACGGGGCCATCGCTCAAGGACGGACCCCTTGTTTCTTCACATTGCTAATTAGCGCAATGAAAAACAAACAAATCTCAACGATGAACAAATATTTTTACTTAAGATTTCAAATATTTGATGTTTTTATGCCAGGTGCCGGAGGTTCATTCATCTGCCTCATCTCTTGCGTTTTATTCATCTCTATCGTGGCCGGAGGCGGCTGCCTGTTGATGTACATGATCCTACCTGAACCTCAAACCACTTGGCTTCCCATTACCGGAGTCGCGTTGGTTTGCCTTCCATGGTTGTTTTGGTTCCTCACATTCCTATATCGACTAATCTCTCGGGGCTCCGGATTTAGAGGTGGCATTGGCAATGCTGCGGCTGTGGCTGGTGGTGCAAAGGTTACTAACACAGCTGCATTGGCATCGAATTTACGTGCCAGTACCGATCTTGCTGCACCTTCTGCTCAATCGCCAGGGGGTGACACTGGTGCTATGGTAGCAAGAGAGGTAGAATTAGGAAGTTTTAAGGATGGAAAGCGAAGGATCTCGTCGTTAAGTCGCAATTCATCAAACATTAATGTATCTCGTCATTCTCATGAGAGTGAAATGCCTTTGGCCTTATCAATGGCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

26.76

Weight (kDa)

9.71

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccII CGCG 1 cut(s) 398
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 4 cut(s) 47, 93, 330, 494
AclWI GGATC 2 cut(s) 346, 668
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 544
AfaI GTAC 3 cut(s) 88, 347, 559
AfiI CCNNNNNNNGG 2 cut(s) 269, 389
AflII CTTAAG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 242
AjnI CCWGG 2 cut(s) 262, 589
AluBI AGCT 1 cut(s) 530
AluI AGCT 1 cut(s) 530
AlwI GGATC 2 cut(s) 346, 668
Ama87I CYCGRG 1 cut(s) 456
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 110, 138, 321, 732
ApeKI GCWGC 4 cut(s) 333, 491, 530, 569
ApoI RAATTY 1 cut(s) 544
AseI ATTAAT 1 cut(s) 693
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 2 cut(s) 138, 156
AsuHPI GGTGA 2 cut(s) 41, 608
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 1 cut(s) 156
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 464
BbvI GCAGC 4 cut(s) 320, 478, 517, 556
BccI CCATC 2 cut(s) 149, 641
BcgI CGANNNNNNTGC 4 cut(s) 522, 551, 556, 585
BciT130I CCWGG 2 cut(s) 264, 591
BfrI CTTAAG 1 cut(s) 232
BisI GCNGC 6 cut(s) 331, 334, 492, 495, 531, 570
BlsI GCNGC 6 cut(s) 332, 335, 493, 496, 532, 571
Bme1390I CCNGG 2 cut(s) 264, 591
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 2 cut(s) 138, 156
BmiI GGNNCC 5 cut(s) 139, 158, 268, 428, 463
BmrFI CCNGG 2 cut(s) 264, 591
BmsI GCATC 1 cut(s) 548
BpuEI CTTGAG 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 413, 590
BsaWI WCCGGW 2 cut(s) 389, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 389
Bse1I ACTGG 2 cut(s) 555, 607
Bse3DI GCAATG 3 cut(s) 173, 195, 493
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 2 cut(s) 264, 591
BseDI CCNNGG 2 cut(s) 413, 590
BseGI GGATG 2 cut(s) 652, 746
BseLI CCNNNNNNNGG 2 cut(s) 269, 389
BseMI GCAATG 3 cut(s) 173, 195, 493
BseNI ACTGG 2 cut(s) 555, 607
BseXI GCAGC 4 cut(s) 320, 478, 517, 556
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 4 cut(s) 112, 140, 323, 734
BshNI GGYRCC 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 4 cut(s) 270, 324, 390, 465
BslI CCNNNNNNNGG 2 cut(s) 269, 389
BsnI GGCC 4 cut(s) 112, 140, 323, 734
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 464
Bsp13I TCCGGA 1 cut(s) 464
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 3 cut(s) 351, 562, 660
Bsp19I CCATGG 1 cut(s) 413
BspACI CCGC 4 cut(s) 47, 93, 330, 494
BspANI GGCC 4 cut(s) 112, 140, 323, 734
BspEI TCCGGA 1 cut(s) 464
BspFNI CGCG 1 cut(s) 398
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 5 cut(s) 139, 158, 268, 428, 463
BspPI GGATC 2 cut(s) 346, 668
BspT107I GGYRCC 1 cut(s) 266
BspTI CTTAAG 1 cut(s) 232
BsrDI GCAATG 3 cut(s) 173, 195, 493
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 2 cut(s) 555, 607
BssECI CCNNGG 2 cut(s) 413, 590
BssMI GATC 3 cut(s) 351, 562, 660
BssT1I CCWWGG 1 cut(s) 413
Bst2UI CCWGG 2 cut(s) 264, 591
BstAFI CTTAAG 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 551
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 2 cut(s) 652, 746
BstFNI CGCG 1 cut(s) 398
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 3 cut(s) 354, 565, 663
BstMBI GATC 3 cut(s) 351, 562, 660
BstMWI GCNNNNNNNGC 5 cut(s) 500, 509, 536, 578, 614
BstNI CCWGG 2 cut(s) 264, 591
BstSCI CCNGG 2 cut(s) 262, 589
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 4 cut(s) 320, 478, 517, 556
BstX2I RGATCY 1 cut(s) 660
BstYI RGATCY 1 cut(s) 660
BsuRI GGCC 4 cut(s) 112, 140, 323, 734
BtgI CCRYGG 1 cut(s) 413
BtgZI GCGATG 1 cut(s) 127
BtsCI GGATG 2 cut(s) 652, 746
BtsIMutI CAGTG 1 cut(s) 600
CciI TCATGA 1 cut(s) 712
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 138, 156
Csp6I GTAC 3 cut(s) 87, 346, 558
CviAII CATG 4 cut(s) 108, 349, 414, 713
CviQI GTAC 3 cut(s) 87, 346, 558
DpnI GATC 3 cut(s) 353, 564, 662
DpnII GATC 3 cut(s) 351, 562, 660
EaeI YGGCCR 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 464
Eco47I GGWCC 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 456
EcoRII CCWGG 2 cut(s) 262, 589
EcoT14I CCWWGG 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 4 cut(s) 111, 352, 417, 716
FaiI YATR 9 cut(s) 11, 26, 109, 260, 350, 415, 442, 611, 714
FatI CATG 4 cut(s) 107, 348, 413, 712
FauI CCCGC 2 cut(s) 54, 86
Fnu4HI GCNGC 6 cut(s) 331, 334, 492, 495, 531, 570
FokI GGATG 2 cut(s) 659, 733
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 6 cut(s) 331, 334, 492, 495, 531, 570
GlaI GCGC 1 cut(s) 187
GluI GCNGC 6 cut(s) 331, 334, 492, 495, 531, 570
HaeIII GGCC 4 cut(s) 112, 140, 323, 734
HapII CCGG 4 cut(s) 270, 324, 390, 465
HhaI GCGC 1 cut(s) 188
Hin1II CATG 4 cut(s) 111, 352, 417, 716
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HinfI GANTC 1 cut(s) 393
HpaII CCGG 4 cut(s) 270, 324, 390, 465
HphI GGTGA 2 cut(s) 41, 608
Hpy166II GTNNAC 1 cut(s) 87
Hpy188III TCNNGA 3 cut(s) 465, 713, 750
Hpy8I GTNNAC 1 cut(s) 87
Hpy99I CGWCG 1 cut(s) 670
HpyAV CCTTC 3 cut(s) 419, 585, 651
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 4 cut(s) 120, 512, 533, 572
HpyF10VI GCNNNNNNNGC 5 cut(s) 500, 509, 536, 578, 614
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 4 cut(s) 111, 352, 417, 716
HspAI GCGC 1 cut(s) 186
Kpn2I TCCGGA 1 cut(s) 464
Kzo9I GATC 3 cut(s) 351, 562, 660
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 4 cut(s) 320, 478, 517, 556
LweI GCATC 1 cut(s) 548
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 2 cut(s) 517, 596
MalI GATC 3 cut(s) 353, 564, 662
MboI GATC 3 cut(s) 351, 562, 660
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 660
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 4 cut(s) 180, 544, 629, 679
MlyI GAGTC 1 cut(s) 402
MnlI CCTC 8 cut(s) 123, 266, 299, 320, 375, 440, 467, 616
MroI TCCGGA 1 cut(s) 464
MseI TTAA 4 cut(s) 233, 642, 671, 693
MslI CAYNNNNRTG 2 cut(s) 711, 717
MspA1I CMGCKG 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 232
MspI CCGG 4 cut(s) 270, 324, 390, 465
MspR9I CCNGG 2 cut(s) 264, 591
MvaI CCWGG 2 cut(s) 264, 591
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 5 cut(s) 500, 509, 536, 578, 614
NcoI CCATGG 1 cut(s) 413
NdeII GATC 3 cut(s) 351, 562, 660
NlaIII CATG 4 cut(s) 111, 352, 417, 716
NlaIV GGNNCC 5 cut(s) 139, 158, 268, 428, 463
NmuCI GTSAC 1 cut(s) 596
PagI TCATGA 1 cut(s) 712
PkrI GCNGC 6 cut(s) 332, 335, 493, 496, 532, 571
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
Ppu21I YACGTR 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 693
Psp6I CCWGG 2 cut(s) 262, 589
PspGI CCWGG 2 cut(s) 262, 589
PspN4I GGNNCC 5 cut(s) 139, 158, 268, 428, 463
PspPI GGNCC 2 cut(s) 138, 156
PsuI RGATCY 1 cut(s) 660
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 88, 347, 559
RsaNI GTAC 3 cut(s) 87, 346, 558
RseI CAYNNNNRTG 2 cut(s) 711, 717
SaqAI TTAA 4 cut(s) 233, 642, 671, 693
SatI GCNGC 6 cut(s) 331, 334, 492, 495, 531, 570
Sau3AI GATC 3 cut(s) 351, 562, 660
Sau96I GGNCC 2 cut(s) 138, 156
SchI GAGTC 1 cut(s) 402
ScrFI CCNGG 2 cut(s) 264, 591
SduI GDGCHC 1 cut(s) 464
SfaNI GCATC 1 cut(s) 548
SinI GGWCC 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 711, 717
SmlI CTYRAG 2 cut(s) 147, 232
SmoI CTYRAG 2 cut(s) 147, 232
Sse9I AATT 4 cut(s) 180, 544, 629, 679
SsiI CCGC 4 cut(s) 47, 93, 330, 494
SspI AATATT 2 cut(s) 224, 246
StyD4I CCNGG 2 cut(s) 262, 589
StyI CCWWGG 1 cut(s) 413
TaiI ACGT 1 cut(s) 553
TaqI TCGA 2 cut(s) 445, 542
TasI AATT 4 cut(s) 180, 544, 629, 679
TatI WGTACW 1 cut(s) 345
TauI GCSGC 2 cut(s) 333, 497
Tru1I TTAA 4 cut(s) 233, 642, 671, 693
Tru9I TTAA 4 cut(s) 233, 642, 671, 693
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 1 cut(s) 596
TseI GCWGC 4 cut(s) 333, 491, 530, 569
Tsp45I GTSAC 1 cut(s) 596
TspDTI ATGAA 6 cut(s) 206, 230, 267, 271, 298, 672
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 156
VspI ATTAAT 1 cut(s) 693
XapI RAATTY 1 cut(s) 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.