MD00G1112500.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008952
Physical Location & Seq
Reverse (-)
23677049 .. 23679547
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1112500.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGGTCGATTTCATCTTATTCTAGTATGGTCACTTTGCATCAGTTTGTGTCTACTCCTTTGTCCCACATCGGCGCAGCTCAAAACAAACTACTATGCCAATATCTGCCCCAACGTTGAAAGCATTGTCAGAGATGCGGTCACTAAGAAATTCCAGCAAACATTCGTCACAGTTCCGGGAACGCTCCGTCTCTTCTTCCACGATTGCTTTGTCGAGGGATGTGATGCTTCTGTTATAGTTGCTTCCACTGCAAACAACAAGGCAGAAAAGGACAATCCAGATAATCTGTCATTAGCCGGAGATGGGTTTGATACGGTGATCAAAGCGAAAGCAGCCGTTGACGCAGTTCCTCAATGCAAAAACAAAGTCTCTTGTGCTGATATTCTTGCCTTGGCCACCAGAGATGTTATTGGTTTGTCTGGGGGACCTTCATATTCTGTTGAGTTGGGAAGATTGGATGGACTAAGCTCAACTTCTACAAGTGTAAATGGGAAGCTACCCAAGTCAACCTTCAATTTGAACCAGCTCAATTCCCTGTTTGCTTCCCATGGGTTATCCCAAGCTGACATGGTTGCTCTTTCAGGGGCACACACCCTTGGGTTCTCTCATTGCAACCAGTTCTCAAATCGGATTTACAGTAACCCAGTGGACCCCACGCTGAACAAAACGTACGCAACGCAGCTCCAACAAATGTGTCCCAAAAATGTAGACCCCGACATAGCCATCGACATGGACCCAACCACGCCCAGGAAGTTTGACAATGTATACTTTCAGAATCTTGTGGAAGGGAAAGGATTATTTACCTCGGACCAGGTCCTTTATACGGACAGCAGATCCCAGCCCACGGTAAGAACGTGGGCCAAAAACAATGCAGCGTTTAACCAGGCCTTTATTACCGCTATGACCAAGCTGGGCCGTGTCGGAATGAAGACCGGCAAGAACGGTAATATTCGTCGCGATTGCAGTGTTTTTAATTAA

Protein Analysis

326

Amino Acids

35.34

Weight (kDa)

8.59

Isoelectric Point (pI)

24.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 43 - 289 2e-79 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49960 AT4G37520 AT4G37520 AT4G37530 AT4G37530 AT5G67400
fragaria_vesca FvH4_1g17340
malus_domestica MD00G1112500.v1.1 MD15G1294200.v1.1
prunus_persica Prupe.6G223400_v2.0.a1
pyrus_communis pycom02g14820 pycom15g25710
rosa_chinensis RchiOBHm_Chr2g0107511
rosa_laevigata RLG00000017587
rosa_multiflora Rmu_sc0022315.1_g000001
rosa_roxburghii Rroxscaffold_2G00136230
rosa_rugosa Rorug02G0144500
rosa_samantha Rh2AG194400 Rh2BG207100 Rh2CG199600 Rh2DG201700
rosa_wichuraiana Rw2G015400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 52, 708, 765
AccII CGCG 1 cut(s) 957
AciI CCGC 2 cut(s) 137, 897
AclI AACGTT 1 cut(s) 114
AclWI GGATC 1 cut(s) 828
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 4 cut(s) 303, 747, 823, 844
AgsI TTSAA 3 cut(s) 119, 514, 520
AjnI CCWGG 3 cut(s) 746, 810, 882
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 7 cut(s) 79, 468, 496, 526, 563, 682, 910
AluI AGCT 7 cut(s) 79, 468, 496, 526, 563, 682, 910
Alw26I GTCTC 2 cut(s) 194, 373
AlwI GGATC 1 cut(s) 828
AoxI GGCC 4 cut(s) 393, 858, 885, 913
ApeKI GCWGC 4 cut(s) 76, 332, 679, 872
ApoI RAATTY 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 7 cut(s) 425, 649, 733, 808, 814, 858, 913
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 328
AvaII GGWCC 5 cut(s) 425, 649, 733, 808, 814
BaeGI GKGCMC 1 cut(s) 589
BalI TGGCCA 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 935
BbvI GCAGC 4 cut(s) 88, 344, 691, 884
BccI CCATC 3 cut(s) 296, 452, 731
BceAI ACGGC 2 cut(s) 320, 900
BciT130I CCWGG 3 cut(s) 748, 812, 884
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 194, 373
BfaI CTAG 1 cut(s) 23
BisI GCNGC 4 cut(s) 77, 333, 680, 873
BlsI GCNGC 4 cut(s) 78, 334, 681, 874
Bme1390I CCNGG 4 cut(s) 177, 748, 812, 884
Bme18I GGWCC 5 cut(s) 425, 649, 733, 808, 814
BmgT120I GGNCC 7 cut(s) 425, 649, 733, 808, 814, 858, 913
BmiI GGNNCC 3 cut(s) 426, 651, 735
BmrFI CCNGG 4 cut(s) 177, 748, 812, 884
BmrI ACTGGG 1 cut(s) 638
BmsI GCATC 3 cut(s) 48, 124, 214
BmuI ACTGGG 1 cut(s) 638
BpiI GAAGAC 1 cut(s) 935
BpuMI CCSGG 1 cut(s) 177
BsaJI CCNNGG 6 cut(s) 390, 547, 595, 746, 804, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 747, 823, 844
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 2 cut(s) 616, 644
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 3 cut(s) 748, 812, 884
BseDI CCNNGG 6 cut(s) 390, 547, 595, 746, 804, 843
BseGI GGATG 2 cut(s) 224, 463
BseLI CCNNNNNNNGG 4 cut(s) 303, 747, 823, 844
BseMI GCAATG 1 cut(s) 607
BseNI ACTGG 2 cut(s) 616, 644
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 4 cut(s) 88, 344, 691, 884
BseYI CCCAGC 2 cut(s) 837, 910
Bsh1236I CGCG 1 cut(s) 957
BshFI GGCC 4 cut(s) 395, 860, 887, 915
BsiSI CCGG 3 cut(s) 176, 297, 933
BsiWI CGTACG 1 cut(s) 669
BslFI GGGAC 3 cut(s) 48, 438, 681
BslI CCNNNNNNNGG 4 cut(s) 303, 747, 823, 844
BsmAI GTCTC 2 cut(s) 194, 373
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 48, 438, 681
BsnI GGCC 4 cut(s) 395, 860, 887, 915
Bsp1286I GDGCHC 1 cut(s) 589
Bsp143I GATC 2 cut(s) 318, 833
Bsp19I CCATGG 1 cut(s) 547
Bsp68I TCGCGA 1 cut(s) 957
BspACI CCGC 2 cut(s) 137, 897
BspANI GGCC 4 cut(s) 395, 860, 887, 915
BspFNI CGCG 1 cut(s) 957
BspLI GGNNCC 3 cut(s) 426, 651, 735
BspPI GGATC 1 cut(s) 828
BsrDI GCAATG 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 932
BsrI ACTGG 2 cut(s) 616, 644
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 6 cut(s) 390, 547, 595, 746, 804, 843
BssMI GATC 2 cut(s) 318, 833
BssNAI GTATAC 1 cut(s) 766
BssT1I CCWWGG 3 cut(s) 390, 547, 595
Bst1107I GTATAC 1 cut(s) 766
Bst2UI CCWGG 3 cut(s) 748, 812, 884
Bst4CI ACNGT 5 cut(s) 172, 316, 638, 847, 944
Bst6I CTCTTC 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 144, 464
BstDSI CCRYGG 2 cut(s) 547, 843
BstF5I GGATG 2 cut(s) 224, 463
BstFNI CGCG 1 cut(s) 957
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 2 cut(s) 321, 836
BstMAI GTCTC 2 cut(s) 194, 373
BstMBI GATC 2 cut(s) 318, 833
BstMWI GCNNNNNNNGC 3 cut(s) 248, 332, 341
BstNI CCWGG 3 cut(s) 748, 812, 884
BstSCI CCNGG 4 cut(s) 175, 746, 810, 882
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 1 cut(s) 957
BstV1I GCAGC 4 cut(s) 88, 344, 691, 884
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 1 cut(s) 833
BstXI CCANNNNNNTGG 1 cut(s) 730
BstYI RGATCY 1 cut(s) 833
BstZ17I GTATAC 1 cut(s) 766
BsuRI GGCC 4 cut(s) 395, 860, 887, 915
BtgI CCRYGG 2 cut(s) 547, 843
BtsCI GGATG 2 cut(s) 224, 463
BtsI GCAGTG 2 cut(s) 246, 970
BtsIMutI CAGTG 3 cut(s) 246, 651, 970
BtuMI TCGCGA 1 cut(s) 957
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 932
Cfr13I GGNCC 7 cut(s) 425, 649, 733, 808, 814, 858, 913
CseI GACGC 1 cut(s) 350
CsiI ACCWGGT 1 cut(s) 810
Csp6I GTAC 1 cut(s) 670
CviAII CATG 3 cut(s) 548, 568, 730
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 2 cut(s) 144, 464
DpnI GATC 2 cut(s) 320, 835
DpnII GATC 2 cut(s) 318, 833
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco130I CCWWGG 3 cut(s) 390, 547, 595
Eco147I AGGCCT 1 cut(s) 887
Eco47I GGWCC 5 cut(s) 425, 649, 733, 808, 814
EcoO109I RGGNCCY 2 cut(s) 425, 814
EcoRII CCWGG 3 cut(s) 746, 810, 882
EcoT14I CCWWGG 3 cut(s) 390, 547, 595
ErhI CCWWGG 3 cut(s) 390, 547, 595
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 3 cut(s) 551, 571, 733
FalI AAGNNNNNCTT 4 cut(s) 457, 489, 494, 526
FaqI GGGAC 3 cut(s) 48, 438, 681
FatI CATG 3 cut(s) 547, 567, 729
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 3 cut(s) 52, 708, 765
Fnu4HI GCNGC 4 cut(s) 77, 333, 680, 873
FokI GGATG 2 cut(s) 231, 470
Fsp4HI GCNGC 4 cut(s) 77, 333, 680, 873
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 77, 333, 680, 873
GsaI CCCAGC 2 cut(s) 841, 914
HaeIII GGCC 4 cut(s) 395, 860, 887, 915
HapII CCGG 3 cut(s) 176, 297, 933
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 76
Hin1II CATG 3 cut(s) 551, 571, 733
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 2 cut(s) 340, 507
HindII GTYRAC 2 cut(s) 340, 507
HinfI GANTC 1 cut(s) 775
HpaII CCGG 3 cut(s) 176, 297, 933
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 6 cut(s) 53, 340, 507, 649, 709, 766
Hpy188I TCNGA 5 cut(s) 131, 630, 774, 808, 923
Hpy188III TCNNGA 2 cut(s) 278, 956
Hpy8I GTNNAC 6 cut(s) 53, 340, 507, 649, 709, 766
Hpy99I CGWCG 1 cut(s) 957
HpyAV CCTTC 3 cut(s) 438, 520, 779
HpyCH4III ACNGT 5 cut(s) 172, 316, 638, 847, 944
HpyCH4IV ACGT 3 cut(s) 114, 668, 854
HpyCH4V TGCA 6 cut(s) 39, 251, 357, 612, 872, 963
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 332, 341
HpyF3I CTNAG 2 cut(s) 144, 464
HpySE526I ACGT 3 cut(s) 114, 668, 854
Hsp92II CATG 3 cut(s) 551, 571, 733
HspAI GCGC 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 2 cut(s) 318, 833
LmnI GCTCC 2 cut(s) 189, 687
Lsp1109I GCAGC 4 cut(s) 88, 344, 691, 884
LweI GCATC 3 cut(s) 48, 124, 214
MabI ACCWGGT 1 cut(s) 810
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 114, 668, 854
MaeIII GTNAC 4 cut(s) 30, 139, 166, 638
MalI GATC 2 cut(s) 320, 835
MboI GATC 2 cut(s) 318, 833
MboII GAAGA 4 cut(s) 184, 187, 462, 940
MflI RGATCY 1 cut(s) 833
MhlI GDGCHC 1 cut(s) 589
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 4 cut(s) 149, 514, 529, 973
MluNI TGGCCA 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 709, 901
MnlI CCTC 3 cut(s) 208, 360, 814
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 879, 972, 976
MslI CAYNNNNRTG 1 cut(s) 728
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 3 cut(s) 176, 297, 933
MspR9I CCNGG 4 cut(s) 177, 748, 812, 884
MvaI CCWGG 3 cut(s) 748, 812, 884
MvnI CGCG 1 cut(s) 957
MwoI GCNNNNNNNGC 3 cut(s) 248, 332, 341
NciI CCSGG 1 cut(s) 177
NcoI CCATGG 1 cut(s) 547
NdeII GATC 2 cut(s) 318, 833
NlaIII CATG 3 cut(s) 551, 571, 733
NlaIV GGNNCC 3 cut(s) 426, 651, 735
NmuCI GTSAC 3 cut(s) 30, 139, 166
NruI TCGCGA 1 cut(s) 957
PacI TTAATTAA 1 cut(s) 976
PceI AGGCCT 1 cut(s) 887
PcsI WCGNNNNNNNCGW 1 cut(s) 851
PfeI GAWTC 1 cut(s) 775
Pfl23II CGTACG 1 cut(s) 669
PflFI GACNNNGTC 1 cut(s) 812
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 4 cut(s) 78, 334, 681, 874
PpuMI RGGWCCY 2 cut(s) 425, 814
Psp1406I AACGTT 1 cut(s) 114
Psp5II RGGWCCY 2 cut(s) 425, 814
Psp6I CCWGG 3 cut(s) 746, 810, 882
PspFI CCCAGC 2 cut(s) 837, 910
PspGI CCWGG 3 cut(s) 746, 810, 882
PspLI CGTACG 1 cut(s) 669
PspN4I GGNNCC 3 cut(s) 426, 651, 735
PspPI GGNCC 7 cut(s) 425, 649, 733, 808, 814, 858, 913
PspPPI RGGWCCY 2 cut(s) 425, 814
PsrI GAACNNNNNNTAC 2 cut(s) 653, 685
PsuI RGATCY 1 cut(s) 833
PsyI GACNNNGTC 1 cut(s) 812
RruI TCGCGA 1 cut(s) 957
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
RseI CAYNNNNRTG 1 cut(s) 728
SaqAI TTAA 3 cut(s) 879, 972, 976
SatI GCNGC 4 cut(s) 77, 333, 680, 873
Sau3AI GATC 2 cut(s) 318, 833
Sau96I GGNCC 7 cut(s) 425, 649, 733, 808, 814, 858, 913
ScrFI CCNGG 4 cut(s) 177, 748, 812, 884
SduI GDGCHC 1 cut(s) 589
SexAI ACCWGGT 1 cut(s) 810
SfaNI GCATC 3 cut(s) 48, 124, 214
SinI GGWCC 5 cut(s) 425, 649, 733, 808, 814
SmiMI CAYNNNNRTG 1 cut(s) 728
Sse9I AATT 4 cut(s) 149, 514, 529, 973
SseBI AGGCCT 1 cut(s) 887
SsiI CCGC 2 cut(s) 137, 897
SspI AATATT 1 cut(s) 949
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 887
StyD4I CCNGG 4 cut(s) 175, 746, 810, 882
StyI CCWWGG 3 cut(s) 390, 547, 595
TaaI ACNGT 5 cut(s) 172, 316, 638, 847, 944
TaiI ACGT 3 cut(s) 117, 671, 857
TaqI TCGA 3 cut(s) 7, 213, 726
TasI AATT 4 cut(s) 149, 514, 529, 973
TfiI GAWTC 1 cut(s) 775
Tru1I TTAA 3 cut(s) 879, 972, 976
Tru9I TTAA 3 cut(s) 879, 972, 976
TscAI CASTG 3 cut(s) 253, 651, 970
TseFI GTSAC 3 cut(s) 30, 139, 166
TseI GCWGC 4 cut(s) 76, 332, 679, 872
Tsp45I GTSAC 3 cut(s) 30, 139, 166
TspDTI ATGAA 2 cut(s) 420, 941
TspGWI ACGGA 2 cut(s) 176, 839
TspRI CASTG 3 cut(s) 253, 651, 970
Tth111I GACNNNGTC 1 cut(s) 812
VpaK11BI GGWCC 5 cut(s) 425, 649, 733, 808, 814
XapI RAATTY 1 cut(s) 149
XmiI GTMKAC 3 cut(s) 52, 708, 765
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.