MD15G1294200.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008952
Physical Location & Seq
Forward (+)
27448281 .. 27450997
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1294200.v1.1.491

Sequence Viewer

Length: 945 bp
ATGTTTGGTCGCATGGGTTGCTTCAATCTTTTACTAGTTTGGTCACTTAGCCTCAGTTTGTGTCTACTTCTTTGTCCCACATCGGCGCAGCTCAAAACAAATTACTATGCCAACATCTGCCCCGACGTAGAAAAAATTGTAAAAAATGCTGTCAATAATAAATTCCAGCAAACATTCGTCACTGGATGTGATGCTTCCGTTATAGTTGCTTCCACTGCAAGCAACAAGGCAGAAAAGGACCATCCTGATAATCTATCATTAGCCGGAGATGGGTTTGATACGGTGATCAAAGCCAAAGCAGCCGTTGATGCAGTTCCTCAATGCAAAAACAAAGTTTCATGCGCTGATATTCTTGCCTTGGCCACCAGAGATGTCATTGGTTTGGTTGGTGGACCTTCATATTCGGTTGAGTTAGGAAGATTGGATGGACTAACCTCAACTTCTACAAGTGTAGATGGGAAGCTGCCCAAGCCAACATTCAATTTGAACCAGCTCACTTCCATGTTTGCTTCCAATGGGTTGTCCCAGGTTGACATGGTTGCTCTTTCAGGGGCACACATCCTAGGGTTCTCTCATTGCAACCAGTTTTCAAATCGCATTTACAGTAATCCAGTGGACCCCACGCTGAACAAAACGTATGCAACGCAGCTCCAACAAATGTGTCCCCAAAATGTGGACCCCGACATAGCCATCAACATGGACCCAACCTCGCCCAGGGAGTTTGACAATGCATACTTTCAGAATCTTATAGAAGGGAAGGGGTTATTTACCTCGGACCAGGTCCTTTTTACGGACAGCAGGTCCCGGCCCACGGTAAGAACATGGGCCAAAGATAAGACGGCTTTTAACCAGGCCTTTATTAACGCTATGACCAAGCTGGGTCGGGTGGGAATCAAGACCGGCAATAATGGCAATATTCGTCGTGATTGCGGTATTTTAAATTGA

Protein Analysis

315

Amino Acids

34.01

Weight (kDa)

8.28

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 49 - 278 4.1e-71 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49960 AT4G37520 AT4G37520 AT4G37530 AT4G37530 AT5G67400
fragaria_vesca FvH4_1g17340
malus_domestica MD00G1112500.v1.1 MD15G1294200.v1.1
prunus_persica Prupe.6G223400_v2.0.a1
pyrus_communis pycom02g14820 pycom15g25710
rosa_chinensis RchiOBHm_Chr2g0107511
rosa_laevigata RLG00000017587
rosa_multiflora Rmu_sc0022315.1_g000001
rosa_roxburghii Rroxscaffold_2G00136230
rosa_rugosa Rorug02G0144500
rosa_samantha Rh2AG194400 Rh2BG207100 Rh2CG199600 Rh2DG201700
rosa_wichuraiana Rw2G015400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
AccB7I CCANNNNNTGG 1 cut(s) 673
AccI GTMKAC 1 cut(s) 64
AciI CCGC 1 cut(s) 930
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 1 cut(s) 161
AfiI CCNNNNNNNGG 5 cut(s) 270, 673, 714, 790, 811
AgsI TTSAA 4 cut(s) 25, 481, 487, 591
AhdI GACNNNNNGTC 1 cut(s) 799
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 4 cut(s) 525, 713, 777, 849
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 5 cut(s) 91, 463, 493, 649, 877
AluI AGCT 5 cut(s) 91, 463, 493, 649, 877
AoxI GGCC 4 cut(s) 360, 806, 825, 852
ApeKI GCWGC 4 cut(s) 88, 299, 463, 646
ApoI RAATTY 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 562
AspLEI GCGC 2 cut(s) 88, 344
AsuC2I CCSGG 1 cut(s) 805
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 8 cut(s) 238, 392, 616, 676, 700, 775, 781, 801
AvrII CCTAGG 1 cut(s) 562
BaeGI GKGCMC 1 cut(s) 556
BalI TGGCCA 1 cut(s) 362
BbvI GCAGC 4 cut(s) 100, 311, 450, 658
BccI CCATC 5 cut(s) 249, 263, 419, 449, 698
BceAI ACGGC 2 cut(s) 287, 855
BciT130I CCWGG 4 cut(s) 527, 715, 779, 851
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 1 cut(s) 805
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 563
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 4 cut(s) 89, 300, 464, 647
BlnI CCTAGG 1 cut(s) 562
BlsI GCNGC 4 cut(s) 90, 301, 465, 648
Bme1390I CCNGG 5 cut(s) 527, 715, 779, 805, 851
Bme18I GGWCC 8 cut(s) 238, 392, 616, 676, 700, 775, 781, 801
BmeRI GACNNNNNGTC 1 cut(s) 799
BmiI GGNNCC 4 cut(s) 618, 678, 702, 803
BmrFI CCNGG 5 cut(s) 527, 715, 779, 805, 851
BmsI GCATC 2 cut(s) 181, 298
BpuMI CCSGG 1 cut(s) 805
BsaJI CCNNGG 7 cut(s) 357, 525, 562, 713, 714, 771, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 270, 673, 714, 790, 811
Bse118I RCCGGY 1 cut(s) 899
Bse1I ACTGG 3 cut(s) 187, 583, 611
Bse3DI GCAATG 1 cut(s) 574
BseBI CCWGG 4 cut(s) 527, 715, 779, 851
BseDI CCNNGG 7 cut(s) 357, 525, 562, 713, 714, 771, 810
BseGI GGATG 4 cut(s) 191, 241, 430, 558
BseLI CCNNNNNNNGG 5 cut(s) 270, 673, 714, 790, 811
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 67
BseNI ACTGG 3 cut(s) 187, 583, 611
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 4 cut(s) 100, 311, 450, 658
BseYI CCCAGC 1 cut(s) 877
BshFI GGCC 4 cut(s) 362, 808, 827, 854
BsiSI CCGG 3 cut(s) 264, 805, 900
BslFI GGGAC 4 cut(s) 60, 508, 648, 787
BslI CCNNNNNNNGG 5 cut(s) 270, 673, 714, 790, 811
BsmFI GGGAC 4 cut(s) 60, 508, 648, 787
BsnI GGCC 4 cut(s) 362, 808, 827, 854
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 1 cut(s) 285
BspACI CCGC 1 cut(s) 930
BspANI GGCC 4 cut(s) 362, 808, 827, 854
BspCNI CTCAG 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 618, 678, 702, 803
BspMI ACCTGC 1 cut(s) 789
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 899
BsrI ACTGG 3 cut(s) 187, 583, 611
BssAI RCCGGY 1 cut(s) 899
BssECI CCNNGG 7 cut(s) 357, 525, 562, 713, 714, 771, 810
BssMI GATC 1 cut(s) 285
BssT1I CCWWGG 2 cut(s) 357, 562
Bst2UI CCWGG 4 cut(s) 527, 715, 779, 851
Bst4CI ACNGT 3 cut(s) 283, 605, 814
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 47, 53
BstDSI CCRYGG 1 cut(s) 810
BstENI CCTNNNNNAGG 1 cut(s) 712
BstF5I GGATG 4 cut(s) 191, 241, 430, 558
BstHHI GCGC 2 cut(s) 88, 344
BstKTI GATC 1 cut(s) 288
BstMBI GATC 1 cut(s) 285
BstMWI GCNNNNNNNGC 6 cut(s) 18, 215, 299, 308, 469, 909
BstNI CCWGG 4 cut(s) 527, 715, 779, 851
BstSCI CCNGG 5 cut(s) 525, 713, 777, 803, 849
BstSLI GKGCMC 1 cut(s) 556
BstV1I GCAGC 4 cut(s) 100, 311, 450, 658
BstXI CCANNNNNNTGG 1 cut(s) 697
BsuRI GGCC 4 cut(s) 362, 808, 827, 854
BtgI CCRYGG 1 cut(s) 810
BtsCI GGATG 4 cut(s) 191, 241, 430, 558
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 3 cut(s) 180, 213, 618
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 220
CfoI GCGC 2 cut(s) 88, 344
Cfr10I RCCGGY 1 cut(s) 899
CsiI ACCWGGT 1 cut(s) 777
CviAII CATG 6 cut(s) 13, 339, 502, 535, 697, 822
DdeI CTNAG 2 cut(s) 47, 53
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
DraI TTTAAA 1 cut(s) 939
DriI GACNNNNNGTC 1 cut(s) 799
EaeI YGGCCR 1 cut(s) 360
Eam1105I GACNNNNNGTC 1 cut(s) 799
Eco130I CCWWGG 2 cut(s) 357, 562
Eco147I AGGCCT 1 cut(s) 854
Eco47I GGWCC 8 cut(s) 238, 392, 616, 676, 700, 775, 781, 801
EcoNI CCTNNNNNAGG 1 cut(s) 712
EcoO109I RGGNCCY 2 cut(s) 781, 801
EcoRII CCWGG 4 cut(s) 525, 713, 777, 849
EcoT14I CCWWGG 2 cut(s) 357, 562
EcoT22I ATGCAT 1 cut(s) 733
ErhI CCWWGG 2 cut(s) 357, 562
FaeI CATG 6 cut(s) 16, 342, 505, 538, 700, 825
FaqI GGGAC 4 cut(s) 60, 508, 648, 787
FatI CATG 6 cut(s) 12, 338, 501, 534, 696, 821
FbaI TGATCA 1 cut(s) 285
FblI GTMKAC 1 cut(s) 64
Fnu4HI GCNGC 4 cut(s) 89, 300, 464, 647
FokI GGATG 4 cut(s) 198, 228, 437, 545
Fsp4HI GCNGC 4 cut(s) 89, 300, 464, 647
FspBI CTAG 2 cut(s) 35, 563
GlaI GCGC 2 cut(s) 87, 343
GluI GCNGC 4 cut(s) 89, 300, 464, 647
GsaI CCCAGC 1 cut(s) 881
HaeIII GGCC 4 cut(s) 362, 808, 827, 854
HapII CCGG 3 cut(s) 264, 805, 900
HhaI GCGC 2 cut(s) 88, 344
Hin1II CATG 6 cut(s) 16, 342, 505, 538, 700, 825
Hin6I GCGC 2 cut(s) 86, 342
HinP1I GCGC 2 cut(s) 86, 342
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 2 cut(s) 742, 891
HpaII CCGG 3 cut(s) 264, 805, 900
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 5 cut(s) 65, 392, 532, 616, 676
Hpy188I TCNGA 2 cut(s) 741, 775
Hpy188III TCNNGA 3 cut(s) 245, 895, 923
Hpy8I GTNNAC 5 cut(s) 65, 392, 532, 616, 676
Hpy99I CGWCG 2 cut(s) 128, 924
HpyAV CCTTC 3 cut(s) 405, 746, 751
HpyCH4III ACNGT 3 cut(s) 283, 605, 814
HpyCH4IV ACGT 2 cut(s) 126, 635
HpyCH4V TGCA 6 cut(s) 218, 311, 324, 579, 641, 731
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 215, 299, 308, 469, 909
HpyF3I CTNAG 2 cut(s) 47, 53
HpySE526I ACGT 2 cut(s) 126, 635
Hsp92II CATG 6 cut(s) 16, 342, 505, 538, 700, 825
HspAI GCGC 2 cut(s) 86, 342
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 654
Lsp1109I GCAGC 4 cut(s) 100, 311, 450, 658
LweI GCATC 2 cut(s) 181, 298
MabI ACCWGGT 1 cut(s) 777
MaeI CTAG 2 cut(s) 35, 563
MaeII ACGT 2 cut(s) 126, 635
MaeIII GTNAC 2 cut(s) 42, 178
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MboII GAAGA 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 556
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 5 cut(s) 100, 135, 161, 481, 940
MluNI TGGCCA 1 cut(s) 362
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 5 cut(s) 62, 327, 445, 718, 781
Mox20I TGGCCA 1 cut(s) 362
Mph1103I ATGCAT 1 cut(s) 733
MscI TGGCCA 1 cut(s) 362
MseI TTAA 3 cut(s) 846, 861, 938
MslI CAYNNNNRTG 2 cut(s) 500, 695
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 3 cut(s) 264, 805, 900
MspR9I CCNGG 5 cut(s) 527, 715, 779, 805, 851
MvaI CCWGG 4 cut(s) 527, 715, 779, 851
MwoI GCNNNNNNNGC 6 cut(s) 18, 215, 299, 308, 469, 909
NciI CCSGG 1 cut(s) 805
NdeII GATC 1 cut(s) 285
NlaIII CATG 6 cut(s) 16, 342, 505, 538, 700, 825
NlaIV GGNNCC 4 cut(s) 618, 678, 702, 803
NmuCI GTSAC 2 cut(s) 42, 178
NsiI ATGCAT 1 cut(s) 733
PasI CCCWGGG 1 cut(s) 714
PceI AGGCCT 1 cut(s) 854
PfeI GAWTC 2 cut(s) 742, 891
PflFI GACNNNGTC 1 cut(s) 779
PflMI CCANNNNNTGG 1 cut(s) 673
PkrI GCNGC 4 cut(s) 90, 301, 465, 648
PpuMI RGGWCCY 2 cut(s) 781, 801
Psp5II RGGWCCY 2 cut(s) 781, 801
Psp6I CCWGG 4 cut(s) 525, 713, 777, 849
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 4 cut(s) 525, 713, 777, 849
PspN4I GGNNCC 4 cut(s) 618, 678, 702, 803
PspPPI RGGWCCY 2 cut(s) 781, 801
PsyI GACNNNGTC 1 cut(s) 779
RseI CAYNNNNRTG 2 cut(s) 500, 695
SaqAI TTAA 3 cut(s) 846, 861, 938
SatI GCNGC 4 cut(s) 89, 300, 464, 647
Sau3AI GATC 1 cut(s) 285
ScrFI CCNGG 5 cut(s) 527, 715, 779, 805, 851
SduI GDGCHC 1 cut(s) 556
SexAI ACCWGGT 1 cut(s) 777
SfaNI GCATC 2 cut(s) 181, 298
SinI GGWCC 8 cut(s) 238, 392, 616, 676, 700, 775, 781, 801
SmiMI CAYNNNNRTG 2 cut(s) 500, 695
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 5 cut(s) 100, 135, 161, 481, 940
SseBI AGGCCT 1 cut(s) 854
SsiI CCGC 1 cut(s) 930
SspI AATATT 1 cut(s) 916
SspMI CTAG 2 cut(s) 35, 563
StuI AGGCCT 1 cut(s) 854
StyD4I CCNGG 5 cut(s) 525, 713, 777, 803, 849
StyI CCWWGG 2 cut(s) 357, 562
TaaI ACNGT 3 cut(s) 283, 605, 814
TaiI ACGT 2 cut(s) 129, 638
TasI AATT 5 cut(s) 100, 135, 161, 481, 940
TfiI GAWTC 2 cut(s) 742, 891
Tru1I TTAA 3 cut(s) 846, 861, 938
Tru9I TTAA 3 cut(s) 846, 861, 938
TscAI CASTG 3 cut(s) 187, 220, 618
TseFI GTSAC 2 cut(s) 42, 178
TseI GCWGC 4 cut(s) 88, 299, 463, 646
Tsp45I GTSAC 2 cut(s) 42, 178
TspDTI ATGAA 2 cut(s) 327, 387
TspGWI ACGGA 2 cut(s) 187, 806
TspRI CASTG 3 cut(s) 187, 220, 618
Tth111I GACNNNGTC 1 cut(s) 779
Van91I CCANNNNNTGG 1 cut(s) 673
VpaK11BI GGWCC 8 cut(s) 238, 392, 616, 676, 700, 775, 781, 801
XagI CCTNNNNNAGG 1 cut(s) 712
XapI RAATTY 1 cut(s) 161
XmaJI CCTAGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 64
XspI CTAG 2 cut(s) 35, 563
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.