MD00G1115800.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012593
Physical Location & Seq
Reverse (-)
24553722 .. 24556302
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1115800.v1.1.491

Sequence Viewer

Length: 873 bp
ATGAATGCTAATGAAGAAATACCTGTGGAAGAAAATGCTGAATCTTTCTTGTCCACTGGTGATGACAATGTTGATGGTACACGCACTCCTTTCAGATTTTTAAGACGTCGTGCTACAGCTTGCAACAAAAATGCACAAAAAGGCTTCACATTTGAAGAAGTTGGTTCCCTCCACTCAAGCAAGTCCAAGGTTGTATGCTGCCATTTTTCATCAGAAGGGAACTTGTTAGCTAGTGCAGGGCATGAGAAAAAGGTTCTGATTTGGAATATGGAAACTTTTGATTTTGTTAAAACTTCAGAAGGGCATTCTCTTCTCATTACAGATGTACGGTTTCAACCAAGCTCAACCGTTTTTGCAACTTCTTCATTTGATAAAACTGTGAAGATATGGGATGCATCAAACGGAGCAACTAAACAAGTCAGATTCCAGCCTCAATTTGGAAAGTTTTTGGCTACTGCTTCAGGAAAAGATATCAATCTATTTGATCTTGAGACCGGACGGATTGAGTTGTTCTTGACGGGATCCGGACACACCAAAGATGTTCTTTCACTTTGCTGGGATCCCACTGGGAAGTATCTTGCCTCTGTTAGTGAAGATAGTGCAAGAGTTTGGTCTCCTGCGTCAAACGGGAAATGCGTATATGAACTGAAATCAAATGGCAACAAATTCCAATCATGCACATTTCATCCCGGATATTCACTGCTCTTGATTATCGGTGGTTACCAGTCCCTTGAGTTGTGGAGTCCAAGCGAAAGCAGCAAGACACTGACAGTTCCGGCGCACCAGGGGATAGTTTCTACGCTGTCAGCATCAGCAAAGACTGAAATGGTTGCCTCAGTGAGCCATGATCAGTGTGTGAAGCTATGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

291

Amino Acids

31.85

Weight (kDa)

6.71

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 39 - 138 9.6e-13 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 44 - 140 2.5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 47 - 136 5.2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 50 - 135 1.6e-07 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 50 - 136 9.4e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 52 - 134 4.7e-10 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 137 2.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 54 - 136 4.2e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 56 - 137 1.3e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 58 - 289 2.2e-28 Prp19 WD40 domain
WD40_Gbeta PF25391 60 - 140 3.2e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 61 - 137 5.8e-15 THOC3 beta-propeller domain
EIF3I PF24805 64 - 137 1.1e-08 EIF3I
WD40 PF00400 95 - 131 2.9e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 137 - 289 1.8e-18 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 138 - 289 2.2e-22 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 138 - 290 2.3e-16 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 139 - 230 5.3e-08 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 153 - 241 3.4e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 174 - 205 1.8e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 180 - 290 1e-08 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 109
AccIII TCCGGA 1 cut(s) 524
AclWI GGATC 4 cut(s) 516, 529, 554, 567
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 2 cut(s) 279, 444
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 2 cut(s) 79, 327
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 2 cut(s) 155, 335
AjnI CCWGG 1 cut(s) 783
AluBI AGCT 4 cut(s) 119, 230, 342, 862
AluI AGCT 4 cut(s) 119, 230, 342, 862
Alw26I GTCTC 2 cut(s) 485, 618
AlwI GGATC 4 cut(s) 516, 529, 554, 567
Aor13HI TCCGGA 1 cut(s) 524
ApeKI GCWGC 2 cut(s) 198, 756
ApoI RAATTY 1 cut(s) 665
AspLEI GCGC 1 cut(s) 781
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 1 cut(s) 71
BamHI GGATCC 2 cut(s) 521, 559
BbvI GCAGC 2 cut(s) 185, 768
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 785
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 2 cut(s) 485, 618
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 2 cut(s) 199, 757
BlsI GCNGC 2 cut(s) 200, 758
Bme1390I CCNGG 2 cut(s) 690, 785
BmiI GGNNCC 3 cut(s) 166, 523, 561
BmrFI CCNGG 2 cut(s) 690, 785
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 3 cut(s) 382, 404, 818
BmuI ACTGGG 1 cut(s) 576
BpuEI CTTGAG 3 cut(s) 160, 509, 752
BpuMI CCSGG 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 106
BsaI GGTCTC 2 cut(s) 485, 618
BsaJI CCNNGG 2 cut(s) 186, 784
BsaWI WCCGGW 2 cut(s) 494, 524
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 3 cut(s) 61, 571, 724
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 2 cut(s) 186, 784
BseGI GGATG 2 cut(s) 397, 685
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMII CTCAG 1 cut(s) 849
BseNI ACTGG 3 cut(s) 61, 571, 724
BseXI GCAGC 2 cut(s) 185, 768
BseYI CCCAGC 1 cut(s) 555
BsgI GTGCAG 1 cut(s) 255
BsiSI CCGG 4 cut(s) 495, 525, 690, 776
BslFI GGGAC 1 cut(s) 712
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 2 cut(s) 485, 618
BsmFI GGGAC 1 cut(s) 712
BsmI GAATGC 2 cut(s) 10, 304
Bso31I GGTCTC 2 cut(s) 485, 618
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 4 cut(s) 484, 521, 559, 847
BspCNI CTCAG 1 cut(s) 848
BspEI TCCGGA 1 cut(s) 524
BspLI GGNNCC 3 cut(s) 166, 523, 561
BspPI GGATC 4 cut(s) 516, 529, 554, 567
BspTNI GGTCTC 2 cut(s) 485, 618
BsrI ACTGG 3 cut(s) 61, 571, 724
BssECI CCNNGG 2 cut(s) 186, 784
BssMI GATC 4 cut(s) 484, 521, 559, 847
BssNI GRCGYC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 785
Bst4CI ACNGT 4 cut(s) 330, 349, 379, 772
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 835
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 2 cut(s) 397, 685
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 4 cut(s) 487, 524, 562, 850
BstMAI GTCTC 2 cut(s) 485, 618
BstMBI GATC 4 cut(s) 484, 521, 559, 847
BstMWI GCNNNNNNNGC 1 cut(s) 756
BstNI CCWGG 1 cut(s) 785
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 2 cut(s) 688, 783
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 185, 768
BstX2I RGATCY 2 cut(s) 521, 559
BstYI RGATCY 2 cut(s) 521, 559
BtsCI GGATG 2 cut(s) 397, 685
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 6 cut(s) 54, 564, 698, 764, 843, 857
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 781
CseI GACGC 1 cut(s) 609
Csp6I GTAC 2 cut(s) 78, 326
CviAII CATG 3 cut(s) 242, 675, 845
CviJI RGCY 8 cut(s) 119, 144, 230, 342, 430, 452, 843, 862
CviKI_1 RGCY 8 cut(s) 119, 144, 230, 342, 430, 452, 843, 862
CviQI GTAC 2 cut(s) 78, 326
DdeI CTNAG 1 cut(s) 835
DpnI GATC 4 cut(s) 486, 523, 561, 849
DpnII GATC 4 cut(s) 484, 521, 559, 847
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 2 cut(s) 485, 618
Eco32I GATATC 1 cut(s) 472
Eco57I CTGAAG 2 cut(s) 279, 444
Eco91I GGTNACC 1 cut(s) 719
EcoO65I GGTNACC 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 783
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 245, 678, 848
FaiI YATR 9 cut(s) 196, 243, 269, 388, 640, 642, 676, 846, 865
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 1 cut(s) 712
FatI CATG 3 cut(s) 241, 674, 844
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 2 cut(s) 199, 757
FokI GGATG 2 cut(s) 404, 672
Fsp4HI GCNGC 2 cut(s) 199, 757
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 780
GluI GCNGC 2 cut(s) 199, 757
GsaI CCCAGC 1 cut(s) 559
HapII CCGG 4 cut(s) 495, 525, 690, 776
HgaI GACGC 1 cut(s) 609
HhaI GCGC 1 cut(s) 781
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 3 cut(s) 245, 678, 848
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HinfI GANTC 3 cut(s) 41, 423, 742
HpaII CCGG 4 cut(s) 495, 525, 690, 776
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 54, 80
Hpy188I TCNGA 5 cut(s) 95, 214, 258, 298, 422
Hpy188III TCNNGA 5 cut(s) 462, 488, 514, 525, 706
Hpy8I GTNNAC 2 cut(s) 54, 80
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 2 cut(s) 209, 293
HpyCH4III ACNGT 4 cut(s) 330, 349, 379, 772
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 7 cut(s) 123, 134, 236, 356, 395, 602, 678
HpyF10VI GCNNNNNNNGC 1 cut(s) 756
HpyF3I CTNAG 1 cut(s) 835
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 3 cut(s) 245, 678, 848
HspAI GCGC 1 cut(s) 779
Kpn2I TCCGGA 1 cut(s) 524
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 4 cut(s) 484, 521, 559, 847
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 2 cut(s) 185, 768
LweI GCATC 3 cut(s) 382, 404, 818
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 719
MalI GATC 4 cut(s) 486, 523, 561, 849
MboI GATC 4 cut(s) 484, 521, 559, 847
MboII GAAGA 7 cut(s) 26, 41, 167, 302, 354, 394, 605
MflI RGATCY 2 cut(s) 521, 559
MluCI AATT 2 cut(s) 434, 665
MlyI GAGTC 1 cut(s) 751
MnlI CCTC 4 cut(s) 179, 441, 592, 844
Mph1103I ATGCAT 1 cut(s) 397
MroI TCCGGA 1 cut(s) 524
MseI TTAA 2 cut(s) 101, 288
MspI CCGG 4 cut(s) 495, 525, 690, 776
MspR9I CCNGG 2 cut(s) 690, 785
Mva1269I GAATGC 2 cut(s) 10, 304
MvaI CCWGG 1 cut(s) 785
MwoI GCNNNNNNNGC 1 cut(s) 756
NciI CCSGG 1 cut(s) 690
NdeII GATC 4 cut(s) 484, 521, 559, 847
NlaIII CATG 3 cut(s) 245, 678, 848
NlaIV GGNNCC 3 cut(s) 166, 523, 561
NsiI ATGCAT 1 cut(s) 397
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 2 cut(s) 10, 304
PfeI GAWTC 2 cut(s) 41, 423
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 2 cut(s) 200, 758
PleI GAGTC 1 cut(s) 750
PpsI GAGTC 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 783
PspEI GGTNACC 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 783
PspN4I GGNNCC 3 cut(s) 166, 523, 561
PsuI RGATCY 2 cut(s) 521, 559
RsaI GTAC 2 cut(s) 79, 327
RsaNI GTAC 2 cut(s) 78, 326
SaqAI TTAA 2 cut(s) 101, 288
SatI GCNGC 2 cut(s) 199, 757
Sau3AI GATC 4 cut(s) 484, 521, 559, 847
SchI GAGTC 1 cut(s) 751
ScrFI CCNGG 2 cut(s) 690, 785
SetI ASST 8 cut(s) 25, 109, 121, 192, 232, 255, 344, 864
SfaNI GCATC 3 cut(s) 382, 404, 818
SfcI CTRYAG 1 cut(s) 114
SmlI CTYRAG 3 cut(s) 175, 488, 731
SmoI CTYRAG 3 cut(s) 175, 488, 731
Sse9I AATT 2 cut(s) 434, 665
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 688, 783
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 330, 349, 379, 772
TaiI ACGT 1 cut(s) 109
TasI AATT 2 cut(s) 434, 665
TfiI GAWTC 2 cut(s) 41, 423
Tru1I TTAA 2 cut(s) 101, 288
Tru9I TTAA 2 cut(s) 101, 288
TscAI CASTG 6 cut(s) 61, 571, 705, 771, 843, 857
TseI GCWGC 2 cut(s) 198, 756
TspDTI ATGAA 6 cut(s) 17, 27, 198, 354, 657, 674
TspGWI ACGGA 2 cut(s) 417, 514
TspRI CASTG 6 cut(s) 61, 571, 705, 771, 843, 857
XapI RAATTY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XspI CTAG 1 cut(s) 231
ZraI GACGTC 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.