Rw5G006200

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012593
Physical Location & Seq
Reverse (-)
6384792 .. 6388209
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006200.1

Sequence Viewer

Length: 1170 bp
ATGGTGGGTCGCCTCTTGAGACAAATTGCAGAGCCCCAGCAACAACCTGATCAACAGCAGTTACTAGAGGGTGACAGAGAGAGGAGGATTGAATCAGGAGCTGGGAGGACAGCATCAGGAGCTGGAGACAGTCATTTGGAAAATAGGAGTGCTAATGAAGATCCCCCTGTGGAAGAAAATACCGAGTCTTTCTTGTCCTATGAGGATGACAATGCTGGTGGTGGTACAAGCACTCTATTCAAGGCTTCAAAGCGTCGTGGTGGGAAAAAAAAGCACGAAAGGGGCTTCAAGTTCGAAGAAATTTGTTCTCTCCATTCAAGCATGGCTAAGGTTATATGCTGCCATTTTTCATCAAATGGGACGTTGTTAGCAAGTGCAGGGCATGAAAAAAAGGTTCTGATTTGGAACATGGAAACTTTTGATGTTGTTAAAACTTTCGAAGAGCATTCTCATCTCATTACAGATGTACGGTTTAGACCAAATTCAACAACGTTTGCAACATCTTCGTTTGATAGAAATGTGAAGATATGGGATGCAGCCAGACCAAGTGAATCAATATCCAAGCTTGAAGGTCACACTGAGCATGTAATGTCAGTGGATTTTCACCCAAGGAAGTTCAATCTCCTTTCATCATGCGATAGTAACGATGAAATCCGACTGTGGAATGTCAACCAATCTAAGTGCACAAGCGTGTTTAAGGGAGCAACTAAACAAGTCAGATTCCAGCCTCGATTTGGAAAATATTTGGCTGCTGCTTCAGGAAATAGTGTCAATACTTTTGATGTTGAGACTGCCAGTTTTGACTTGTATTCGAAGGGATCTGGACATGCCAAAGATGTCCTTTCAATTTGCTGGGATACAAGTGGGAAGTATCTTGCCTCTGTTAGTGAAGACAGTGCACGGATTTGGTCAACTGCATCAAGCGGAAAATGCATATATGAACTCCACTCAAATGGCAACAAATTCCAATCATGCACATTTCATCCTGGATATTCACTACTCTTGATCATTGGTTGTTACGAGTCCCTTGAGTTGTGGAGTCCAAGTGAGAGCAATAAAACTATGACTGTTCCAGCGCACAAGGGGCTAGTTGCTGCACTGGCGTCATCCCCAAAGACTGACATGGTTGCCTCTGGGAGCCATGATCAGGTCGTCAAACTATGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

389

Amino Acids

43.06

Weight (kDa)

6.95

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 96 - 300 1.7e-13 MABP1/WDR62 second WD40 domain
EIF3I PF24805 97 - 180 1.5e-07 EIF3I
Beta-prop_THOC3 PF25174 98 - 179 3.8e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 99 - 239 2.5e-22 WDR3 first beta-propeller domain
WDR55 PF24796 99 - 228 4e-09 WDR55
WD40_CDC20-Fz PF24807 101 - 231 5e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 102 - 223 3.3e-16 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 105 - 228 5.1e-18 Prp19 WD40 domain
Beta-prop_Aladin PF25460 106 - 306 1.4e-09 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd PF25168 106 - 181 5.8e-09 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 106 - 181 2e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 107 - 239 2.2e-29 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 108 - 227 1.6e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EIPR1 PF23609 121 - 221 1e-05 EIPR1 beta-propeller
Beta-prop_IFT122_1st PF23381 131 - 291 1e-05 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 137 - 232 4.7e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 139 - 237 1.9e-06 TEP-1 second beta-propeller
WD40 PF00400 142 - 178 4.6e-08 WD domain, G-beta repeat
WD40_Gbeta PF25391 147 - 245 2.1e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 149 - 263 1.1e-17 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 153 - 332 1e-19 WDHD1 first WD40 domain
WD40_Prp19 PF24814 184 - 257 1e-06 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 188 - 336 3e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 212 - 388 1.8e-17 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 215 - 389 1.2e-18 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 234 - 312 1.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 236 - 388 7.9e-22 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 238 - 388 4.2e-17 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 247 - 385 3.3e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 279 - 389 1.2e-10 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 924
AclI AACGTT 1 cut(s) 491
AclWI GGATC 2 cut(s) 155, 826
AcsI RAATTY 3 cut(s) 300, 481, 962
AcuI CTGAAG 1 cut(s) 741
AcyI GRCGYC 1 cut(s) 1103
AfaI GTAC 2 cut(s) 226, 468
AfiI CCNNNNNNNGG 2 cut(s) 734, 1147
AgsI TTSAA 9 cut(s) 92, 241, 249, 289, 318, 486, 569, 619, 846
AjnI CCWGG 1 cut(s) 985
AleI CACNNNNGTG 1 cut(s) 689
AluBI AGCT 3 cut(s) 101, 122, 565
AluI AGCT 3 cut(s) 101, 122, 565
Alw21I GWGCWC 2 cut(s) 686, 901
Alw26I GTCTC 3 cut(s) 13, 120, 782
Alw44I GTGCAC 2 cut(s) 682, 897
AlwI GGATC 2 cut(s) 155, 826
AlwNI CAGNNNCTG 2 cut(s) 101, 122
ApaLI GTGCAC 2 cut(s) 682, 897
ApeKI GCWGC 5 cut(s) 339, 536, 749, 752, 1094
ApoI RAATTY 3 cut(s) 300, 481, 962
AspLEI GCGC 1 cut(s) 1078
AsuHPI GGTGA 2 cut(s) 83, 596
AsuII TTCGAA 3 cut(s) 294, 438, 812
BaeGI GKGCMC 2 cut(s) 686, 901
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 2 cut(s) 686, 901
BbvI GCAGC 5 cut(s) 326, 548, 736, 739, 1081
BcgI CGANNNNNNTGC 2 cut(s) 486, 520
BciT130I CCWGG 1 cut(s) 987
BciVI GTATCC 1 cut(s) 850
BclI TGATCA 3 cut(s) 49, 1005, 1144
BcoDI GTCTC 3 cut(s) 13, 120, 782
BfaI CTAG 2 cut(s) 65, 1088
BfuI GTATCC 1 cut(s) 850
BisI GCNGC 5 cut(s) 340, 537, 750, 753, 1095
BlsI GCNGC 5 cut(s) 341, 538, 751, 754, 1096
Bme1390I CCNGG 1 cut(s) 987
BmiI GGNNCC 1 cut(s) 1139
BmrFI CCNGG 1 cut(s) 987
BmsI GCATC 3 cut(s) 122, 523, 926
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 327
Bpu14I TTCGAA 3 cut(s) 294, 438, 812
BpuEI CTTGAG 2 cut(s) 37, 1049
BsaHI GRCGYC 1 cut(s) 1103
BsaJI CCNNGG 1 cut(s) 608
BsaXI ACNNNNNCTCC 2 cut(s) 1030, 1060
Bsc4I CCNNNNNNNGG 2 cut(s) 734, 1147
Bse1I ACTGG 2 cut(s) 795, 1104
BseBI CCWGG 1 cut(s) 987
BseDI CCNNGG 1 cut(s) 608
BseGI GGATG 4 cut(s) 211, 538, 982, 1106
BseLI CCNNNNNNNGG 2 cut(s) 734, 1147
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 2 cut(s) 795, 1104
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 2 cut(s) 686, 901
BseXI GCAGC 5 cut(s) 326, 548, 736, 739, 1081
BseYI CCCAGC 3 cut(s) 36, 101, 852
BsgI GTGCAG 2 cut(s) 396, 1080
BsiHKAI GWGCWC 2 cut(s) 686, 901
BslFI GGGAC 2 cut(s) 373, 1009
BslI CCNNNNNNNGG 2 cut(s) 734, 1147
BsmAI GTCTC 3 cut(s) 13, 120, 782
BsmFI GGGAC 2 cut(s) 373, 1009
BsmI GAATGC 1 cut(s) 445
Bsp119I TTCGAA 3 cut(s) 294, 438, 812
Bsp1286I GDGCHC 3 cut(s) 36, 686, 901
Bsp143I GATC 5 cut(s) 49, 160, 818, 1005, 1144
BspACI CCGC 1 cut(s) 924
BspCNI CTCAG 1 cut(s) 571
BspLI GGNNCC 1 cut(s) 1139
BspPI GGATC 2 cut(s) 155, 826
BspQI GCTCTTC 1 cut(s) 435
BspT104I TTCGAA 3 cut(s) 294, 438, 812
BsrI ACTGG 2 cut(s) 795, 1104
BssECI CCNNGG 1 cut(s) 608
BssMI GATC 5 cut(s) 49, 160, 818, 1005, 1144
BssNI GRCGYC 1 cut(s) 1103
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 987
Bst4CI ACNGT 5 cut(s) 131, 471, 660, 896, 1069
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 1 cut(s) 1103
BstBI TTCGAA 3 cut(s) 294, 438, 812
BstDEI CTNAG 3 cut(s) 327, 579, 678
BstF5I GGATG 4 cut(s) 211, 538, 982, 1106
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 5 cut(s) 52, 163, 821, 1008, 1147
BstMAI GTCTC 3 cut(s) 13, 120, 782
BstMBI GATC 5 cut(s) 49, 160, 818, 1005, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 119, 930, 1084, 1100
BstNI CCWGG 1 cut(s) 987
BstNSI RCATGY 2 cut(s) 587, 830
BstSCI CCNGG 1 cut(s) 985
BstSLI GKGCMC 2 cut(s) 686, 901
BstV1I GCAGC 5 cut(s) 326, 548, 736, 739, 1081
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 2 cut(s) 160, 818
BstXI CCANNNNNNTGG 1 cut(s) 953
BstYI RGATCY 2 cut(s) 160, 818
BsuI GTATCC 1 cut(s) 850
BtsCI GGATG 4 cut(s) 211, 538, 982, 1106
BtsIMutI CAGTG 4 cut(s) 576, 600, 901, 1097
CaiI CAGNNNCTG 2 cut(s) 101, 122
CfoI GCGC 1 cut(s) 1078
CseI GACGC 2 cut(s) 242, 1092
Csp6I GTAC 2 cut(s) 225, 467
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 9 cut(s) 322, 383, 409, 584, 633, 827, 972, 1123, 1142
CviQI GTAC 2 cut(s) 225, 467
DdeI CTNAG 3 cut(s) 327, 579, 678
DpnI GATC 5 cut(s) 51, 162, 820, 1007, 1146
DpnII GATC 5 cut(s) 49, 160, 818, 1005, 1144
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 741
EcoRII CCWGG 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 608
EcoT22I ATGCAT 1 cut(s) 935
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 9 cut(s) 325, 386, 412, 587, 636, 830, 975, 1126, 1145
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 2 cut(s) 373, 1009
FatI CATG 9 cut(s) 321, 382, 408, 583, 632, 826, 971, 1122, 1141
FbaI TGATCA 3 cut(s) 49, 1005, 1144
Fnu4HI GCNGC 5 cut(s) 340, 537, 750, 753, 1095
FokI GGATG 4 cut(s) 218, 545, 969, 1093
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 5 cut(s) 340, 537, 750, 753, 1095
FspBI CTAG 2 cut(s) 65, 1088
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 5 cut(s) 340, 537, 750, 753, 1095
GsaI CCCAGC 3 cut(s) 40, 105, 856
GsuI CTGGAG 1 cut(s) 144
HgaI GACGC 2 cut(s) 242, 1092
HhaI GCGC 1 cut(s) 1078
Hin1I GRCGYC 1 cut(s) 1103
Hin1II CATG 9 cut(s) 325, 386, 412, 587, 636, 830, 975, 1126, 1145
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HincII GTYRAC 2 cut(s) 670, 912
HindII GTYRAC 2 cut(s) 670, 912
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 6 cut(s) 92, 185, 551, 720, 1022, 1039
HphI GGTGA 2 cut(s) 83, 596
Hpy166II GTNNAC 4 cut(s) 670, 684, 899, 912
Hpy188I TCNGA 3 cut(s) 399, 656, 719
Hpy188III TCNNGA 6 cut(s) 16, 96, 117, 759, 822, 1003
Hpy8I GTNNAC 4 cut(s) 670, 684, 899, 912
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 563, 808
HpyCH4III ACNGT 5 cut(s) 131, 471, 660, 896, 1069
HpyCH4IV ACGT 2 cut(s) 362, 491
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 930, 1084, 1100
HpyF3I CTNAG 3 cut(s) 327, 579, 678
HpySE526I ACGT 2 cut(s) 362, 491
Hsp92I GRCGYC 1 cut(s) 1103
Hsp92II CATG 9 cut(s) 325, 386, 412, 587, 636, 830, 975, 1126, 1145
HspAI GCGC 1 cut(s) 1076
Ksp22I TGATCA 3 cut(s) 49, 1005, 1144
Kzo9I GATC 5 cut(s) 49, 160, 818, 1005, 1144
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 4 cut(s) 98, 119, 701, 1137
Lsp1109I GCAGC 5 cut(s) 326, 548, 736, 739, 1081
LweI GCATC 3 cut(s) 122, 523, 926
MaeI CTAG 2 cut(s) 65, 1088
MaeII ACGT 2 cut(s) 362, 491
MaeIII GTNAC 5 cut(s) 60, 71, 572, 641, 1016
MalI GATC 5 cut(s) 51, 162, 820, 1007, 1146
MboI GATC 5 cut(s) 49, 160, 818, 1005, 1144
MboII GAAGA 7 cut(s) 170, 185, 308, 452, 495, 535, 902
MflI RGATCY 2 cut(s) 160, 818
MhlI GDGCHC 3 cut(s) 36, 686, 901
MluCI AATT 5 cut(s) 24, 300, 481, 846, 962
MlyI GAGTC 3 cut(s) 194, 1031, 1048
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 9 cut(s) 23, 61, 75, 78, 99, 196, 738, 889, 1141
Mph1103I ATGCAT 1 cut(s) 935
MseI TTAA 2 cut(s) 429, 696
MslI CAYNNNNRTG 2 cut(s) 689, 951
MspR9I CCNGG 1 cut(s) 987
Mva1269I GAATGC 1 cut(s) 445
MvaI CCWGG 1 cut(s) 987
MwoI GCNNNNNNNGC 4 cut(s) 119, 930, 1084, 1100
NdeII GATC 5 cut(s) 49, 160, 818, 1005, 1144
NlaIII CATG 9 cut(s) 325, 386, 412, 587, 636, 830, 975, 1126, 1145
NlaIV GGNNCC 1 cut(s) 1139
NmuCI GTSAC 2 cut(s) 71, 572
NsiI ATGCAT 1 cut(s) 935
NspI RCATGY 2 cut(s) 587, 830
NspV TTCGAA 3 cut(s) 294, 438, 812
OliI CACNNNNGTG 1 cut(s) 689
PciSI GCTCTTC 1 cut(s) 435
PctI GAATGC 1 cut(s) 445
PfeI GAWTC 3 cut(s) 92, 551, 720
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 5 cut(s) 341, 538, 751, 754, 1096
PleI GAGTC 3 cut(s) 193, 1030, 1047
PpsI GAGTC 3 cut(s) 193, 1030, 1047
Psp1406I AACGTT 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 985
PspFI CCCAGC 3 cut(s) 36, 101, 852
PspGI CCWGG 1 cut(s) 985
PspN4I GGNNCC 1 cut(s) 1139
PstNI CAGNNNCTG 2 cut(s) 101, 122
PsuI RGATCY 2 cut(s) 160, 818
RsaI GTAC 2 cut(s) 226, 468
RsaNI GTAC 2 cut(s) 225, 467
RseI CAYNNNNRTG 2 cut(s) 689, 951
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 2 cut(s) 429, 696
SatI GCNGC 5 cut(s) 340, 537, 750, 753, 1095
Sau3AI GATC 5 cut(s) 49, 160, 818, 1005, 1144
SchI GAGTC 3 cut(s) 194, 1031, 1048
ScrFI CCNGG 1 cut(s) 987
SduI GDGCHC 3 cut(s) 36, 686, 901
SfaNI GCATC 3 cut(s) 122, 523, 926
SfuI TTCGAA 3 cut(s) 294, 438, 812
SmiMI CAYNNNNRTG 2 cut(s) 689, 951
SmlI CTYRAG 2 cut(s) 16, 1028
SmoI CTYRAG 2 cut(s) 16, 1028
Sse9I AATT 5 cut(s) 24, 300, 481, 846, 962
SsiI CCGC 1 cut(s) 924
SspI AATATT 1 cut(s) 743
SspMI CTAG 2 cut(s) 65, 1088
StyD4I CCNGG 1 cut(s) 985
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 5 cut(s) 131, 471, 660, 896, 1069
TaiI ACGT 2 cut(s) 365, 494
TaqI TCGA 4 cut(s) 294, 438, 730, 812
TasI AATT 5 cut(s) 24, 300, 481, 846, 962
TfiI GAWTC 3 cut(s) 92, 551, 720
Tru1I TTAA 2 cut(s) 429, 696
Tru9I TTAA 2 cut(s) 429, 696
TscAI CASTG 4 cut(s) 583, 600, 901, 1104
TseFI GTSAC 2 cut(s) 71, 572
TseI GCWGC 5 cut(s) 339, 536, 749, 752, 1094
Tsp45I GTSAC 2 cut(s) 71, 572
TspDTI ATGAA 7 cut(s) 171, 339, 399, 618, 663, 954, 971
TspGWI ACGGA 1 cut(s) 916
TspRI CASTG 4 cut(s) 583, 600, 901, 1104
VneI GTGCAC 2 cut(s) 682, 897
XapI RAATTY 3 cut(s) 300, 481, 962
XceI RCATGY 2 cut(s) 587, 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XspI CTAG 2 cut(s) 65, 1088
Zsp2I ATGCAT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.