MD00G1130100.v1.1

Signal peptidase complex catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012805
Physical Location & Seq
Forward (+)
27997101 .. 28008990
11890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1130100.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGGTTGGATCGGTGAGAGCGTGGACTCCATCAAATCCCTTCAGATCCGGCAGGTCCTCACCCAGGCCGTCAGTCTCGGTTCGTCAACCCTTAGACCCTCGCTCTCGCTCGCCAATTTTCTCCTTATATCGGAAATTCTTCCTCCGTTATTTCAATTGGATTTTGGAAGAGCTTTAATGTGTGTCACCGGCAGTGAGTCTCCTGTAGTTGTTGTTTTGTCTGGAAGTATGGAACCTGGTTTCAAGAGGGGGGACATTTTATTCCTGCACATGAGTAAAGAACCCATTCGTGCAGGAGAAATTGTTGTTTTTAATATTGATGGCCGTGAAATTCCAATTGTCCATCGTGTCATTAAGGTTCATGAACGAAAAGATACAGGAGAAGTTGATGTCCTAACAAAAGGAGATAACAATTTTGGGGATGACAGGCTTCTGTATGCTCAGGGTCAGCTGTGGCTACAACGGCATCATATCATGGGCAGAGCTGTAGGGTTTTTACCTTATGTTGGTTGGGTGACAATTATCATGACTGAAAAACCTATTATTAAGTATATTCTAATTGGCGCGTTGGGGTTGCTTGTTATAACATCAAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
Peptidase_S24

Protein Analysis

200

Amino Acids

22.03

Weight (kDa)

6.97

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S24 PF00717 60 - 122 4e-09 Peptidase S24-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52600
fragaria_vesca FvH4_6g30010 FvH4_7g29810
malus_domestica MD00G1130100.v1.1 MD17G1208500.v1.1
prunus_persica Prupe.3G083100_v2.0.a1 Prupe.3G083100_v2.0.a1
pyrus_communis pycom09g13940 pycom17g21120
rosa_chinensis RchiOBHm_Chr2g0136041
rosa_laevigata RLG00000019498
rosa_multiflora Rmu_sc0011078.1_g000006
rosa_roxburghii Rroxscaffold_2G00108630
rosa_rugosa Rorug02G0327800
rosa_samantha Rh2AG379500 Rh2BG386000 Rh2CG365400
rosa_wichuraiana Rw2G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 43
AccII CGCG 1 cut(s) 566
AclWI GGATC 2 cut(s) 17, 40
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 2 cut(s) 135, 330
AcuI CTGAAG 1 cut(s) 26
AfiI CCNNNNNNNGG 3 cut(s) 64, 130, 506
AgsI TTSAA 2 cut(s) 155, 244
AjnI CCWGG 2 cut(s) 63, 235
AluBI AGCT 3 cut(s) 173, 451, 485
AluI AGCT 3 cut(s) 173, 451, 485
Alw26I GTCTC 2 cut(s) 80, 204
AlwI GGATC 2 cut(s) 17, 40
AoxI GGCC 2 cut(s) 66, 322
ApoI RAATTY 2 cut(s) 135, 330
Asp700I GAANNNNTTC 2 cut(s) 138, 285
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 4 cut(s) 26, 52, 178, 526
AvaII GGWCC 1 cut(s) 55
BccI CCATC 3 cut(s) 38, 314, 351
BceAI ACGGC 3 cut(s) 53, 309, 479
BciT130I CCWGG 2 cut(s) 65, 237
BcoDI GTCTC 2 cut(s) 80, 204
BfmI CTRYAG 2 cut(s) 204, 486
BfuAI ACCTGC 1 cut(s) 43
Bme1390I CCNGG 2 cut(s) 65, 237
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 65, 237
BmsI GCATC 1 cut(s) 475
Bpu10I CCTNAGC 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 130, 506
Bse118I RCCGGY 1 cut(s) 188
BseBI CCWGG 2 cut(s) 65, 237
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 3 cut(s) 64, 130, 506
BseMII CTCAG 1 cut(s) 455
BsgI GTGCAG 2 cut(s) 251, 312
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 2 cut(s) 68, 324
BsiSI CCGG 2 cut(s) 49, 189
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 64, 130, 506
BsmAI GTCTC 2 cut(s) 80, 204
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 68, 324
Bsp143I GATC 2 cut(s) 9, 45
BspANI GGCC 2 cut(s) 68, 324
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 566
BspHI TCATGA 2 cut(s) 361, 525
BspLI GGNNCC 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 17, 40
BspQI GCTCTTC 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 2 cut(s) 9, 45
Bst2UI CCWGG 2 cut(s) 65, 237
Bst6I CTCTTC 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 2 cut(s) 92, 441
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 2 cut(s) 12, 48
BstMAI GTCTC 2 cut(s) 80, 204
BstMBI GATC 2 cut(s) 9, 45
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstNI CCWGG 2 cut(s) 65, 237
BstSCI CCNGG 2 cut(s) 63, 235
BstSFI CTRYAG 2 cut(s) 204, 486
BstUI CGCG 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 2 cut(s) 68, 324
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 199
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 111
CciI TCATGA 2 cut(s) 361, 525
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 235
CviAII CATG 4 cut(s) 271, 362, 475, 526
CviJI RGCY 7 cut(s) 68, 173, 324, 430, 451, 457, 485
CviKI_1 RGCY 7 cut(s) 68, 173, 324, 430, 451, 457, 485
DdeI CTNAG 2 cut(s) 92, 441
DpnI GATC 2 cut(s) 11, 47
DpnII GATC 2 cut(s) 9, 45
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 63, 235
FaeI CATG 4 cut(s) 274, 365, 478, 529
FaqI GGGAC 1 cut(s) 266
FatI CATG 4 cut(s) 270, 361, 474, 525
FokI GGATG 1 cut(s) 434
GlaI GCGC 1 cut(s) 565
HaeIII GGCC 2 cut(s) 68, 324
HapII CCGG 2 cut(s) 49, 189
HhaI GCGC 1 cut(s) 566
Hin1II CATG 4 cut(s) 274, 365, 478, 529
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 2 cut(s) 26, 197
HpaII CCGG 2 cut(s) 49, 189
HphI GGTGA 4 cut(s) 26, 52, 178, 526
Hpy166II GTNNAC 2 cut(s) 25, 87
Hpy188I TCNGA 2 cut(s) 45, 133
Hpy188III TCNNGA 4 cut(s) 222, 244, 362, 526
Hpy8I GTNNAC 2 cut(s) 25, 87
HpyAV CCTTC 1 cut(s) 50
HpyCH4V TGCA 2 cut(s) 268, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 2 cut(s) 92, 441
Hsp92II CATG 4 cut(s) 274, 365, 478, 529
HspAI GCGC 1 cut(s) 564
Kzo9I GATC 2 cut(s) 9, 45
LguI GCTCTTC 1 cut(s) 163
LweI GCATC 1 cut(s) 475
MabI ACCWGGT 1 cut(s) 235
MaeIII GTNAC 2 cut(s) 184, 514
MalI GATC 2 cut(s) 11, 47
MboI GATC 2 cut(s) 9, 45
MboII GAAGA 2 cut(s) 131, 180
MfeI CAATTG 2 cut(s) 155, 336
MflI RGATCY 1 cut(s) 45
MluCI AATT 9 cut(s) 115, 135, 155, 300, 330, 336, 412, 519, 558
MlyI GAGTC 2 cut(s) 20, 206
MnlI CCTC 4 cut(s) 68, 109, 153, 240
MroXI GAANNNNTTC 2 cut(s) 138, 285
MseI TTAA 4 cut(s) 176, 312, 354, 546
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 2 cut(s) 49, 189
MspR9I CCNGG 2 cut(s) 65, 237
MunI CAATTG 2 cut(s) 155, 336
MvaI CCWGG 2 cut(s) 65, 237
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 1 cut(s) 463
NdeII GATC 2 cut(s) 9, 45
NlaIII CATG 4 cut(s) 274, 365, 478, 529
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 2 cut(s) 184, 514
PagI TCATGA 2 cut(s) 361, 525
PciSI GCTCTTC 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PdmI GAANNNNTTC 2 cut(s) 138, 285
PleI GAGTC 2 cut(s) 20, 205
PpsI GAGTC 2 cut(s) 20, 205
PpuMI RGGWCCY 1 cut(s) 55
PsiI TTATAA 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 63, 235
PspGI CCWGG 2 cut(s) 63, 235
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 55
PspPPI RGGWCCY 1 cut(s) 55
PsuI RGATCY 1 cut(s) 45
PvuII CAGCTG 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 163
SaqAI TTAA 4 cut(s) 176, 312, 354, 546
Sau3AI GATC 2 cut(s) 9, 45
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 2 cut(s) 20, 206
ScrFI CCNGG 2 cut(s) 65, 237
SetI ASST 8 cut(s) 57, 175, 238, 360, 453, 487, 502, 541
SexAI ACCWGGT 1 cut(s) 235
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 2 cut(s) 204, 486
SinI GGWCC 1 cut(s) 55
Sse9I AATT 9 cut(s) 115, 135, 155, 300, 330, 336, 412, 519, 558
SspI AATATT 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 63, 235
TasI AATT 9 cut(s) 115, 135, 155, 300, 330, 336, 412, 519, 558
Tru1I TTAA 4 cut(s) 176, 312, 354, 546
Tru9I TTAA 4 cut(s) 176, 312, 354, 546
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 184, 514
Tsp45I GTSAC 2 cut(s) 184, 514
TspDTI ATGAA 2 cut(s) 350, 378
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 55
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 135, 330
XmnI GAANNNNTTC 2 cut(s) 138, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.