Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012067

Physical Location & Seq

Genomic Coordinates Forward (+)

39904383 .. 39910793

Gene Structure 6411 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1172700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3492 bp

ATGGTGGCGGGAGTTGTTGATCCCTCAATCGTCTCCCCATTTTATTATAAAGCGCCAAAACCCTTACCCTACCCTCAGACACAGGAAGGAAGCAGAGACAAGAGAAGAGAAATGGCGACTCTGAGCTCTGCCAAGAAGTCGTACGAGTACGCCAAGGCAGAAGGGACCCGTCTTCAGGAGGCTCAGTGGGCAAACGTGATCGGAAACATGTTGAAGAAGAGTGGGAAGTACGCAGAGGCCCTCAAGTGGCTAAACATCGACTACGATCTTTCCATCAAGTACTTGTCCCAAAGCCACTGCCTCCCCGCCTGTCAGTCACTTGGCGAGCTCTATCTTTGCCTTGAAGTTTTCCAACATGCTCTTCTTTTTCAGAAAAAACATTTACAGCTTGCTGAGGATGCCAATGATCTTGTCGAGCAGCAAAGAGCAAATACCCAACTAGGCCGAACCTACCATGAAATGTTTTTAAGATCCGAGGATGACCACTTCTCTGTTCAAAATGCCCGAAAGTATTTCAAAGCTGCAATGAAGCTTGCACACATTATTAAGGAAAGCCCACCTACTAATAATTGTTCTTTCCTAGAGGAATACATTGACGCTCATAACAATATAGGAATGCTTGAGTTTGATCTTGATAATCTGGAAGAGGCTCGGAAAATATTGACTAAAGGGTTGGAGATTTGCGATGAACGAGGGGTAGTGGAGGATGATGATGGGCGCAGCAGGCTCCATCACAATCTTGGAAATGTTTACATGAAGCTGAGGATGTGGGATAGTGCTCGAGAACACATTGATAAGGACATTTTGATATGTCAAAGAACTGAACATCGTCAAGGGGAAGCAAAGGGGTACATCAATCTTGGTGAATTGCATTATCGTGTTCAGAACTATCAGGAGGCACTCCGATGCTACCAAAAAGCTCTTGACCTGACAAATCTAATGGAAGGTGAGGATCCTCTTCTCAAGCAAATTCATCAAAATATTGGAACTGTAAAAAAAGCCGCTGAAGTAATGAACGAGTTGCAAAAAGAAGAGCAGAATCTCAAAAAGCTAACAGAAGACAAAGAAGATGCAAGAGGCAGACCACGTGAGAGGCATTGTCTGCCGCAGATAATTGCCTCTCTTGACTGTCTCATTGAGAAGTCAAGGACGACCATTGCCTGGGCAAAGCTTTGTGAGTTTGCAAGCATGAAGAAGATAATATCAAGTGAACTTCACGACAAACAAAAGCTCATTGATTCATTTTTGGTTATTGGAGAATCATACGAGAACTTAAGAGAATTCGACAAAGCCTTGGACTGGTATACGAGGAGTTGGAAAGAGTACCAGTTCATTGGTGACTTTGAGGGGCAGGCATTAGCGAAAGTTAATATAGGTAATGTTTTGGACCACACTAACAACTGGCAAGGAGCATTAGACGCATTCAAAGAAAGCTACAAGCTTGCTGTTAGGGCTAACCTTCCTTCTATACAGCTCACTGCTCTAGAGAATATGCACTACAGCTACATGATCAGATTTGACAAAGCTGAAGAGGCCAGGAGAGTGAAGCTTCTAGTTGAGGAATTGAAACAACAAAGAAATAAAGAGTTTCATACAGAAAATGTTCCAGAGGATTTGTGTTCCAGTTCTAACAGTCGTTCTAAATGTGCTACCCTTGTCTCTGAAAATATAGTGCATAGGAATGCTGTTTCCCGTCCCATTTTTCTTTCTTCTGCTGATAAACACAATTGTCAAGTTGTTTCATTTAAGATCGACAAAGACGTAATACATTACCCAATGAAGGCTACTGGGAAGTTAAGCATTGAGTCTATGAAGGTTGAACTAGCATGCTTATATTACTTGGGACTTCCTGTGGAAAGAAGATCAGACGGAATTCTTCCAATAATTAAGGTCATAAAGCTTGGTGGACGAGTTATCGAATCCCTAGAAACACTTGAAGCAACGGTTTCAGAGCATATGGGACAAGTCTTGTTTGATGCTTGCATTGGTGGATGGAATCAGAAGCGCTTGATAAAACTATACACCGATTGCTGCAACACGTTATGTGAGACGCCCAATATGAAAGTGCTTATGAAACTATACGATCAGGAGGTAAGTCCAGAGGCAAGTGTATTAAGGCAAAAATTTGACATCCTTGTTATGTCTTTCACAACCTACGACATGATGCTTAGCAGATACGTTATGACAGATGCTGACGTTGCTGTGTCTGGATGTGGTTTGCAAGATTTTTCAATAACTCCATTGTTGAATGCCTTAGATGCCTATAAAGACTTTGCAATGCTAGACCTTTCTCACAATTTGTTAGGAAATAGTACGATGGAGAAGCTGCAGAAAGTATTACCAGGCTATACATCTTATGGGTTAAGGTTGGATATACACTGCAACCTATTTGGTCCAACTGCTTTGCTCCAGATATGTGAATGCCCTGTGCTCGTTGCTCGATTGGAAGTGCTAAATATATCCGAGAACCCTCTCACTGATGCATGCGCATCTTATCTTTTAGATGTCTTGGAAAAGTGCAAGACCCTTTACAGCTTGAGTATGGAGCGCTGCTGTATCACATCTAGAACAGTTCTTATGGTTGCTGATGCATTAAACGCTGGATCTCCACTTGACCAACTTTGTATAGGATATAACGACATTGATGACAATGCCCTTGCTTGTTTACTTGTCAAGCTTGGCTCTCTGAAAAGATTTTCACGTTTGAATCTGAACGGTTTGGAGCTAAACAAGCGTGCAGTTGATAGCCTTTGCGGCCTTGCTTCGACCTTGCCCTTGTTCGAACTAATGCTTCGGGAAACTGGTATTGGAATGGATGGAGCGTCATTAGTAACCAATTCATTGTTCATCAGGACTGAGGAGATTTTCAAACTTGACTTATCGTATTGTGGACTGACGTCTAATTATGCTCTGAAAATTAGCACTAATTCTTGTATGATTTGTGGCATTTACGAGCTGAACCTTTCAGGAAATCCTATCAAGGAAGAGGGTAGCAAAGCGTTATCGTCGATGCTTTTGAATTCCAAATGTTGTCTGCAAGTTTTGGTCCTTGAGAAGTGCGAGCTTGGAGTTGCTGGGTTTCTTCAAATAATCCAGGCAGTATCAGGTTCCCTCACTAATTCTTATGTTGAGGAGCTCAATCTCGCCGATAATGTCAAATCTCTTCATGTTGCTGATCAAGTTGGGCTCGACGATGACTGCAAAACCACATGCCAAGGAGAATTCTCATTCATTCAAAAGGTTTCAACTGCCATTCGCAAGGCAACGAATTTGCGATTCCTGGACCTTAGCAAAAATGGTTTTTCCAAACAAAGTGCAGAGGTATTTTATTCCGCGTGGTCGAGATCAAGACCGAGTGACAGTTCCTTGTATGGTTCACGTGGTTGGCATATGGAGGAGGAGACCATCCATTTGTTTTTGGAGGGAATCAAGTGCTGTGTGAAACCTTGCTGCAAAACGAACAGCTTTTCTTGCTCTGCTTATCGCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000278 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007049 GO:0008150 GO:0009653 GO:0009888 GO:0009933 GO:0009934 GO:0009987 GO:0016020 GO:0022402 GO:0022603 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0048507 GO:0048509 GO:0048532 GO:0048856 GO:0050789 GO:0050793 GO:0051301 GO:0065007 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_6 TPR_10 TPR_12 TPR_2 TPR_7 TPR_8

Protein Analysis

1164

Amino Acids

131.49

Weight (kDa)

6.01

Isoelectric Point (pI)

44.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_12	PF13424	199 - 263	1.8e-06	Tetratricopeptide repeat
TPR_12	PF13424	242 - 310	4.5e-11	Tetratricopeptide repeat
TPR_2	PF07719	281 - 310	8.5e-07	Tetratricopeptide repeat
TPR_1	PF00515	281 - 310	2.4e-06	Tetratricopeptide repeat
TPR_12	PF13424	282 - 333	1.5e-06	Tetratricopeptide repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18730
fragaria_vesca	FvH4_1g23980 FvH4_1g23980 FvH4_1g23980
malus_domestica	MD00G1172700.v1.1 MD09G1255500.v1.1 MD17G1248600.v1.1
prunus_persica	Prupe.3G147500_v2.0.a1 Prupe.3G147500_v2.0.a1
pyrus_communis	pycom09g17130
rosa_chinensis	RchiOBHm_Chr2g0120111
rosa_laevigata	RLG00000018521
rosa_multiflora	Rmu_sc0010863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123720
rosa_rugosa	Rorug02G0226800
rosa_samantha	Rh2AG283400 Rh2BG294700 Rh2DG308400
rosa_wichuraiana	Rw2G022700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	48
AatII	GACGTC	1 cut(s)	2900
Acc16I	TGCGCA	1 cut(s)	2488
AccB7I	CCANNNNNTGG	1 cut(s)	1161
AccI	GTMKAC	1 cut(s)	1304
AccII	CGCG	1 cut(s)	3335
AciI	CCGC	6 cut(s)	8, 306, 1002, 1106, 2754, 3333
AclWI	GGATC	5 cut(s)	14, 465, 947, 960, 2611
AcsI	RAATTY	7 cut(s)	969, 1280, 1872, 2123, 3019, 3221, 3268
AcuI	CTGAAG	3 cut(s)	158, 1026, 1548
AcvI	CACGTG	2 cut(s)	1088, 3380
AcyI	GRCGYC	2 cut(s)	2051, 2897
AfaI	GTAC	7 cut(s)	143, 149, 230, 281, 851, 1325, 2314
AfeI	AGCGCT	2 cut(s)	2006, 2549
AfiI	CCNNNNNNNGG	5 cut(s)	175, 246, 1161, 1299, 1780
AflII	CTTAAG	1 cut(s)	1273
AflIII	ACRYGT	2 cut(s)	207, 2037
AjnI	CCWGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
AjuI	GAANNNNNNNTTGG	6 cut(s)	345, 377, 2790, 2822, 2828, 2860
Alw21I	GWGCWC	5 cut(s)	128, 330, 781, 2433, 3138
Alw26I	GTCTC	6 cut(s)	37, 90, 1136, 1663, 2042, 3395
AlwI	GGATC	5 cut(s)	14, 465, 947, 960, 2611
AlwNI	CAGNNNCTG	1 cut(s)	2192
Ama87I	CYCGRG	1 cut(s)	780
Aor51HI	AGCGCT	2 cut(s)	2006, 2549
AoxI	GGCC	4 cut(s)	237, 442, 1533, 2755
ApeKI	GCWGC	7 cut(s)	418, 521, 720, 2031, 2326, 2550, 3450
ApoI	RAATTY	7 cut(s)	969, 1280, 1872, 2123, 3019, 3221, 3268
ArsI	GACNNNNNNTTYG	2 cut(s)	3394, 3426
Asp700I	GAANNNNTTC	1 cut(s)	1602
AspLEI	GCGC	5 cut(s)	55, 720, 2007, 2489, 2550
AspS9I	GGNCC	6 cut(s)	165, 238, 1387, 2393, 3046, 3283
AsuHPI	GGTGA	3 cut(s)	875, 959, 1349
AsuII	TTCGAA	1 cut(s)	2781
AvaI	CYCGRG	1 cut(s)	780
AvaII	GGWCC	5 cut(s)	165, 1387, 2393, 3046, 3283
BamHI	GGATCC	1 cut(s)	952
BanII	GRGCYC	4 cut(s)	128, 330, 3138, 3189
BbrPI	CACGTG	2 cut(s)	1088, 3380
BbsI	GAAGAC	2 cut(s)	164, 1065
Bbv12I	GWGCWC	5 cut(s)	128, 330, 781, 2433, 3138
BbvCI	CCTCAGC	2 cut(s)	393, 761
BbvI	GCAGC	7 cut(s)	430, 508, 732, 2018, 2313, 2537, 3437
BccI	CCATC	7 cut(s)	281, 707, 738, 1986, 2311, 2810, 3413
BciT130I	CCWGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
BclI	TGATCA	2 cut(s)	1509, 3175
BcoDI	GTCTC	6 cut(s)	37, 90, 1136, 1663, 2042, 3395
BfaI	CTAG	8 cut(s)	440, 581, 1484, 1553, 1823, 1925, 2282, 2565
BfmI	CTRYAG	2 cut(s)	1498, 2327
BfoI	RGCGCY	3 cut(s)	56, 2008, 2551
BfrI	CTTAAG	1 cut(s)	1273
BglI	GCCNNNNNGGC	1 cut(s)	2754
BlpI	GCTNAGC	1 cut(s)	2168
BmcAI	AGTACT	1 cut(s)	281
Bme1390I	CCNGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
Bme18I	GGWCC	5 cut(s)	165, 1387, 2393, 3046, 3283
BmeT110I	CYCGRG	1 cut(s)	780
BmgT120I	GGNCC	6 cut(s)	165, 238, 1387, 2393, 3046, 3283
BmiI	GGNNCC	5 cut(s)	166, 167, 728, 954, 3109
BmrFI	CCNGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
BmrI	ACTGGG	1 cut(s)	1797
BmuI	ACTGGG	1 cut(s)	1797
BoxI	GACNNNNGTC	1 cut(s)	2896
BpiI	GAAGAC	2 cut(s)	164, 1065
BpmI	CTGGAG	1 cut(s)	2393
Bpu10I	CCTNAGC	3 cut(s)	393, 761, 3287
Bpu1102I	GCTNAGC	1 cut(s)	2168
Bpu14I	TTCGAA	1 cut(s)	2781
BpuEI	CTTGAG	5 cut(s)	227, 641, 947, 2557, 3071
BsaAI	YACGTR	2 cut(s)	1088, 3380
BsaHI	GRCGYC	2 cut(s)	2051, 2897
BsaI	GGTCTC	1 cut(s)	3395
BsaJI	CCNNGG	5 cut(s)	153, 474, 1161, 1293, 3214
BsaXI	ACNNNNNCTCC	2 cut(s)	170, 200
Bsc4I	CCNNNNNNNGG	5 cut(s)	175, 246, 1161, 1299, 1780
Bse1I	ACTGG	7 cut(s)	1304, 1327, 1406, 1623, 1792, 2806, 3487
Bse3DI	GCAATG	3 cut(s)	531, 1155, 2283
BseBI	CCWGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
BseDI	CCNNGG	5 cut(s)	153, 474, 1161, 1293, 3214
BseGI	GGATG	9 cut(s)	403, 484, 712, 771, 1997, 2130, 2216, 2821, 3405
BseLI	CCNNNNNNNGG	5 cut(s)	175, 246, 1161, 1299, 1780
BseMI	GCAATG	3 cut(s)	531, 1155, 2283
BseMII	CTCAG	6 cut(s)	89, 113, 197, 384, 752, 2847
BseNI	ACTGG	7 cut(s)	1304, 1327, 1406, 1623, 1792, 2806, 3487
BseRI	GAGGAG	5 cut(s)	1324, 2873, 3146, 3410, 3413
BseXI	GCAGC	7 cut(s)	430, 508, 732, 2018, 2313, 2537, 3437
BseYI	CCCAGC	1 cut(s)	3074
BsgI	GTGCAG	2 cut(s)	2757, 3336
Bsh1236I	CGCG	1 cut(s)	3335
BshFI	GGCC	4 cut(s)	239, 444, 1535, 2757
BsiHKAI	GWGCWC	5 cut(s)	128, 330, 781, 2433, 3138
BsiHKCI	CYCGRG	1 cut(s)	780
BsiWI	CGTACG	1 cut(s)	141
BslFI	GGGAC	5 cut(s)	178, 271, 1680, 1857, 1974
BslI	CCNNNNNNNGG	5 cut(s)	175, 246, 1161, 1299, 1780
BsmAI	GTCTC	6 cut(s)	37, 90, 1136, 1663, 2042, 3395
BsmBI	CGTCTC	2 cut(s)	37, 2042
BsmFI	GGGAC	5 cut(s)	178, 271, 1680, 1857, 1974
BsmI	GAATGC	5 cut(s)	621, 1421, 1687, 2254, 2426
BsnI	GGCC	4 cut(s)	239, 444, 1535, 2757
Bso31I	GGTCTC	1 cut(s)	3395
BsoBI	CYCGRG	1 cut(s)	780
Bsp119I	TTCGAA	1 cut(s)	2781
Bsp1286I	GDGCHC	6 cut(s)	128, 330, 781, 2433, 3138, 3189
Bsp1720I	GCTNAGC	1 cut(s)	2168
BspACI	CCGC	6 cut(s)	8, 306, 1002, 1106, 2754, 3333
BspANI	GGCC	4 cut(s)	239, 444, 1535, 2757
BspCNI	CTCAG	6 cut(s)	88, 114, 196, 385, 753, 2848
BspFNI	CGCG	1 cut(s)	3335
BspLI	GGNNCC	5 cut(s)	166, 167, 728, 954, 3109
BspMAI	CTGCAG	1 cut(s)	2331
BspPI	GGATC	5 cut(s)	14, 465, 947, 960, 2611
BspQI	GCTCTTC	2 cut(s)	366, 1026
BspT104I	TTCGAA	1 cut(s)	2781
BspTI	CTTAAG	1 cut(s)	1273
BspTNI	GGTCTC	1 cut(s)	3395
BsrDI	GCAATG	3 cut(s)	531, 1155, 2283
BsrI	ACTGG	7 cut(s)	1304, 1327, 1406, 1623, 1792, 2806, 3487
BssECI	CCNNGG	5 cut(s)	153, 474, 1161, 1293, 3214
BssNAI	GTATAC	1 cut(s)	1305
BssNI	GRCGYC	2 cut(s)	2051, 2897
BssT1I	CCWWGG	3 cut(s)	153, 1293, 3214
Bst1107I	GTATAC	1 cut(s)	1305
Bst2UI	CCWGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
Bst4CI	ACNGT	7 cut(s)	991, 1130, 1634, 1945, 2572, 2717, 3362
Bst6I	CTCTTC	9 cut(s)	100, 212, 366, 639, 963, 1026, 1524, 2979, 3168
BstACI	GRCGYC	2 cut(s)	2051, 2897
BstAFI	CTTAAG	1 cut(s)	1273
BstAPI	GCANNNNNTGC	1 cut(s)	1102
BstBAI	YACGTR	2 cut(s)	1088, 3380
BstBI	TTCGAA	1 cut(s)	2781
BstDEI	CTNAG	9 cut(s)	75, 122, 183, 393, 761, 2168, 2254, 2856, 3287
BstF5I	GGATG	9 cut(s)	403, 484, 712, 771, 1997, 2130, 2216, 2821, 3405
BstFNI	CGCG	1 cut(s)	3335
BstH2I	RGCGCY	3 cut(s)	56, 2008, 2551
BstHHI	GCGC	5 cut(s)	55, 720, 2007, 2489, 2550
BstMAI	GTCTC	6 cut(s)	37, 90, 1136, 1663, 2042, 3395
BstNI	CCWGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
BstNSI	RCATGY	5 cut(s)	211, 359, 1830, 2487, 3213
BstPAI	GACNNNNGTC	1 cut(s)	2896
BstSCI	CCNGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
BstSFI	CTRYAG	2 cut(s)	1498, 2327
BstUI	CGCG	1 cut(s)	3335
BstV1I	GCAGC	7 cut(s)	430, 508, 732, 2018, 2313, 2537, 3437
BstV2I	GAAGAC	2 cut(s)	164, 1065
BstX2I	RGATCY	3 cut(s)	470, 952, 2603
BstXI	CCANNNNNNTGG	1 cut(s)	1334
BstYI	RGATCY	3 cut(s)	470, 952, 2603
BstZ17I	GTATAC	1 cut(s)	1305
BsuRI	GGCC	4 cut(s)	239, 444, 1535, 2757
BtgZI	GCGATG	1 cut(s)	699
BtsCI	GGATG	9 cut(s)	403, 484, 712, 771, 1997, 2130, 2216, 2821, 3405
BtsI	GCAGTG	3 cut(s)	295, 1476, 2377
BtsIMutI	CAGTG	5 cut(s)	191, 295, 1476, 2377, 2475
CaiI	CAGNNNCTG	1 cut(s)	2192
CfoI	GCGC	5 cut(s)	55, 720, 2007, 2489, 2550
Cfr13I	GGNCC	6 cut(s)	165, 238, 1387, 2393, 3046, 3283
CseI	GACGC	4 cut(s)	605, 1427, 2059, 2811
Csp6I	GTAC	7 cut(s)	142, 148, 229, 280, 850, 1324, 2313
CviQI	GTAC	7 cut(s)	142, 148, 229, 280, 850, 1324, 2313
DdeI	CTNAG	9 cut(s)	75, 122, 183, 393, 761, 2168, 2254, 2856, 3287
Eam1104I	CTCTTC	9 cut(s)	100, 212, 366, 639, 963, 1026, 1524, 2979, 3168
EarI	CTCTTC	9 cut(s)	100, 212, 366, 639, 963, 1026, 1524, 2979, 3168
Ecl136II	GAGCTC	3 cut(s)	126, 328, 3136
Eco130I	CCWWGG	3 cut(s)	153, 1293, 3214
Eco24I	GRGCYC	4 cut(s)	128, 330, 3138, 3189
Eco31I	GGTCTC	1 cut(s)	3395
Eco47I	GGWCC	5 cut(s)	165, 1387, 2393, 3046, 3283
Eco47III	AGCGCT	2 cut(s)	2006, 2549
Eco53kI	GAGCTC	3 cut(s)	126, 328, 3136
Eco57I	CTGAAG	3 cut(s)	158, 1026, 1548
Eco72I	CACGTG	2 cut(s)	1088, 3380
Eco88I	CYCGRG	1 cut(s)	780
EcoICRI	GAGCTC	3 cut(s)	126, 328, 3136
EcoO109I	RGGNCCY	2 cut(s)	165, 238
EcoRI	GAATTC	4 cut(s)	1280, 1872, 3019, 3221
EcoRII	CCWGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
EcoT14I	CCWWGG	3 cut(s)	153, 1293, 3214
EcoT22I	ATGCAT	2 cut(s)	2485, 2593
EcoT38I	GRGCYC	4 cut(s)	128, 330, 3138, 3189
ErhI	CCWWGG	3 cut(s)	153, 1293, 3214
Esp3I	CGTCTC	2 cut(s)	37, 2042
FaqI	GGGAC	5 cut(s)	178, 271, 1680, 1857, 1974
FauI	CCCGC	1 cut(s)	313
FauNDI	CATATG	2 cut(s)	1956, 3390
FbaI	TGATCA	2 cut(s)	1509, 3175
FblI	GTMKAC	1 cut(s)	1304
FokI	GGATG	9 cut(s)	410, 491, 719, 778, 2004, 2117, 2223, 2828, 3392
FriOI	GRGCYC	4 cut(s)	128, 330, 3138, 3189
FspAI	RTGCGCAY	1 cut(s)	2488
FspBI	CTAG	8 cut(s)	440, 581, 1484, 1553, 1823, 1925, 2282, 2565
FspI	TGCGCA	1 cut(s)	2488
GlaI	GCGC	5 cut(s)	54, 719, 2006, 2488, 2549
GsaI	CCCAGC	1 cut(s)	3078
GsuI	CTGGAG	1 cut(s)	2393
HaeII	RGCGCY	3 cut(s)	56, 2008, 2551
HaeIII	GGCC	4 cut(s)	239, 444, 1535, 2757
HgaI	GACGC	4 cut(s)	605, 1427, 2059, 2811
HhaI	GCGC	5 cut(s)	55, 720, 2007, 2489, 2550
Hin1I	GRCGYC	2 cut(s)	2051, 2897
Hin6I	GCGC	5 cut(s)	53, 718, 2005, 2487, 2548
HinP1I	GCGC	5 cut(s)	53, 718, 2005, 2487, 2548
HindIII	AAGCTT	6 cut(s)	530, 1169, 1439, 1547, 1898, 2675
HphI	GGTGA	3 cut(s)	875, 959, 1349
Hpy166II	GTNNAC	7 cut(s)	751, 1211, 1305, 1907, 2666, 2891, 3377
Hpy8I	GTNNAC	7 cut(s)	751, 1211, 1305, 1907, 2666, 2891, 3377
Hpy99I	CGWCG	2 cut(s)	3010, 3194
HpyAV	CCTTC	7 cut(s)	80, 155, 938, 1469, 1473, 1774, 1807
HpyCH4III	ACNGT	7 cut(s)	991, 1130, 1634, 1945, 2572, 2717, 3362
HpyCH4IV	ACGT	9 cut(s)	195, 1087, 1761, 2039, 2178, 2196, 2701, 2897, 3379
HpyF3I	CTNAG	9 cut(s)	75, 122, 183, 393, 761, 2168, 2254, 2856, 3287
HpySE526I	ACGT	9 cut(s)	195, 1087, 1761, 2039, 2178, 2196, 2701, 2897, 3379
Hsp92I	GRCGYC	2 cut(s)	2051, 2897
HspAI	GCGC	5 cut(s)	53, 718, 2005, 2487, 2548
KflI	GGGWCCC	1 cut(s)	165
Ksp22I	TGATCA	2 cut(s)	1509, 3175
LguI	GCTCTTC	2 cut(s)	366, 1026
LmnI	GCTCC	7 cut(s)	732, 1409, 2412, 2545, 2722, 2819, 3133
Lsp1109I	GCAGC	7 cut(s)	430, 508, 732, 2018, 2313, 2537, 3437
MaeI	CTAG	8 cut(s)	440, 581, 1484, 1553, 1823, 1925, 2282, 2565
MaeII	ACGT	9 cut(s)	195, 1087, 1761, 2039, 2178, 2196, 2701, 2897, 3379
MaeIII	GTNAC	4 cut(s)	315, 1337, 2830, 3356
MfeI	CAATTG	1 cut(s)	1726
MflI	RGATCY	3 cut(s)	470, 952, 2603
MhlI	GDGCHC	6 cut(s)	128, 330, 781, 2433, 3138, 3189
MlyI	GAGTC	2 cut(s)	112, 1814
MmeI	TCCRAC	5 cut(s)	376, 654, 1295, 2349, 2420
Mph1103I	ATGCAT	2 cut(s)	2485, 2593
MroXI	GAANNNNTTC	1 cut(s)	1602
MslI	CAYNNNNRTG	3 cut(s)	876, 904, 1680
MspA1I	CMGCKG	1 cut(s)	1004
MspCI	CTTAAG	1 cut(s)	1273
MspR9I	CCNGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
MunI	CAATTG	1 cut(s)	1726
Mva1269I	GAATGC	5 cut(s)	621, 1421, 1687, 2254, 2426
MvaI	CCWGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
MvnI	CGCG	1 cut(s)	3335
NdeI	CATATG	2 cut(s)	1956, 3390
NlaIV	GGNNCC	5 cut(s)	166, 167, 728, 954, 3109
NmuCI	GTSAC	3 cut(s)	315, 1337, 3356
NsbI	TGCGCA	1 cut(s)	2488
NsiI	ATGCAT	2 cut(s)	2485, 2593
NspI	RCATGY	5 cut(s)	211, 359, 1830, 2487, 3213
NspV	TTCGAA	1 cut(s)	2781
PaeI	GCATGC	2 cut(s)	1830, 2487
PaeR7I	CTCGAG	1 cut(s)	780
PciI	ACATGT	1 cut(s)	207
PciSI	GCTCTTC	2 cut(s)	366, 1026
PcsI	WCGNNNNNNNCGW	1 cut(s)	1758
PctI	GAATGC	5 cut(s)	621, 1421, 1687, 2254, 2426
PdmI	GAANNNNTTC	1 cut(s)	1602
PfeI	GAWTC	8 cut(s)	1039, 1238, 1259, 1919, 1996, 2707, 3276, 3426
Pfl23II	CGTACG	1 cut(s)	141
PflMI	CCANNNNNTGG	1 cut(s)	1161
PfoI	TCCNGGA	1 cut(s)	3279
PleI	GAGTC	2 cut(s)	112, 1813
PmaCI	CACGTG	2 cut(s)	1088, 3380
PmlI	CACGTG	2 cut(s)	1088, 3380
PpsI	GAGTC	2 cut(s)	112, 1813
Ppu21I	YACGTR	2 cut(s)	1088, 3380
PpuMI	RGGWCCY	1 cut(s)	165
PscI	ACATGT	1 cut(s)	207
PshAI	GACNNNNGTC	1 cut(s)	2896
PsiI	TTATAA	1 cut(s)	48
Psp124BI	GAGCTC	3 cut(s)	128, 330, 3138
Psp5II	RGGWCCY	1 cut(s)	165
Psp6I	CCWGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
PspCI	CACGTG	2 cut(s)	1088, 3380
PspFI	CCCAGC	1 cut(s)	3074
PspGI	CCWGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
PspLI	CGTACG	1 cut(s)	141
PspN4I	GGNNCC	5 cut(s)	166, 167, 728, 954, 3109
PspPI	GGNCC	6 cut(s)	165, 238, 1387, 2393, 3046, 3283
PspPPI	RGGWCCY	1 cut(s)	165
PstI	CTGCAG	1 cut(s)	2331
PstNI	CAGNNNCTG	1 cut(s)	2192
PsuI	RGATCY	3 cut(s)	470, 952, 2603
RsaI	GTAC	7 cut(s)	143, 149, 230, 281, 851, 1325, 2314
RsaNI	GTAC	7 cut(s)	142, 148, 229, 280, 850, 1324, 2313
RseI	CAYNNNNRTG	3 cut(s)	876, 904, 1680
SacI	GAGCTC	3 cut(s)	128, 330, 3138
SapI	GCTCTTC	2 cut(s)	366, 1026
Sau96I	GGNCC	6 cut(s)	165, 238, 1387, 2393, 3046, 3283
ScaI	AGTACT	1 cut(s)	281
SchI	GAGTC	2 cut(s)	112, 1814
ScrFI	CCNGG	5 cut(s)	1162, 1537, 2343, 3095, 3281
SduI	GDGCHC	6 cut(s)	128, 330, 781, 2433, 3138, 3189
SfcI	CTRYAG	2 cut(s)	1498, 2327
Sfr274I	CTCGAG	1 cut(s)	780
SfuI	TTCGAA	1 cut(s)	2781
SinI	GGWCC	5 cut(s)	165, 1387, 2393, 3046, 3283
SlaI	CTCGAG	1 cut(s)	780
SmiMI	CAYNNNNRTG	3 cut(s)	876, 904, 1680
SmlI	CTYRAG	7 cut(s)	242, 620, 780, 962, 1273, 2536, 3050
SmoI	CTYRAG	7 cut(s)	242, 620, 780, 962, 1273, 2536, 3050
SphI	GCATGC	2 cut(s)	1830, 2487
SsiI	CCGC	6 cut(s)	8, 306, 1002, 1106, 2754, 3333
SspI	AATATT	2 cut(s)	660, 982
SspMI	CTAG	8 cut(s)	440, 581, 1484, 1553, 1823, 1925, 2282, 2565
SstI	GAGCTC	3 cut(s)	128, 330, 3138
StyD4I	CCNGG	5 cut(s)	1160, 1535, 2341, 3093, 3279
StyI	CCWWGG	3 cut(s)	153, 1293, 3214
TaaI	ACNGT	7 cut(s)	991, 1130, 1634, 1945, 2572, 2717, 3362
TaiI	ACGT	9 cut(s)	198, 1090, 1764, 2042, 2181, 2199, 2704, 2900, 3382
TaqII	GACCGA	1 cut(s)	3367
TatI	WGTACW	1 cut(s)	279
TauI	GCSGC	3 cut(s)	1004, 1108, 2757
TfiI	GAWTC	8 cut(s)	1039, 1238, 1259, 1919, 1996, 2707, 3276, 3426
TscAI	CASTG	5 cut(s)	191, 302, 1483, 2384, 2482
TseFI	GTSAC	3 cut(s)	315, 1337, 3356
TseI	GCWGC	7 cut(s)	418, 521, 720, 2031, 2326, 2550, 3450
Tsp45I	GTSAC	3 cut(s)	315, 1337, 3356
TspGWI	ACGGA	1 cut(s)	1884
TspRI	CASTG	5 cut(s)	191, 302, 1483, 2384, 2482
Van91I	CCANNNNNTGG	1 cut(s)	1161
Vha464I	CTTAAG	1 cut(s)	1273
VpaK11BI	GGWCC	5 cut(s)	165, 1387, 2393, 3046, 3283
XapI	RAATTY	7 cut(s)	969, 1280, 1872, 2123, 3019, 3221, 3268
XbaI	TCTAGA	2 cut(s)	1483, 2564
XceI	RCATGY	5 cut(s)	211, 359, 1830, 2487, 3213
XcmI	CCANNNNNNNNNTGG	1 cut(s)	737
XhoI	CTCGAG	1 cut(s)	780
XmiI	GTMKAC	1 cut(s)	1304
XmnI	GAANNNNTTC	1 cut(s)	1602
XspI	CTAG	8 cut(s)	440, 581, 1484, 1553, 1823, 1925, 2282, 2565
ZraI	GACGTC	1 cut(s)	2898
ZrmI	AGTACT	1 cut(s)	281
Zsp2I	ATGCAT	2 cut(s)	2485, 2593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.