Rw2G022700

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012067
Physical Location & Seq
Forward (+)
32545596 .. 32557035
11440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022700.1

Sequence Viewer

Length: 3942 bp
ATGGCAAGGGAGGAAGCGAAGCTGAGTGAGGCGAAGGCGTCTTACCGGAGCGCGAAGGCCATCGGCAACCACGAGGAGGAGGCCCGGTGGGCCAACGTCATCGGCAACTTGTTGAAGGACAGAGGCGAGTACCTGCAGGCCCTCAAGTGGTTCGACATCGATTACCATCTCTGCACCAAGTACTTGTCCCCCAAGCACTCCCTTGCCACCTGTCAGTCCCTCGGTGAGGTCTATCTCCGTCTCGAGCGTTTCGAAGACGCCCTCGTGTATCAGAAAAAACATTTGATGCTTGCTGAGGAGGCCAATGACCTTGTTGAGCAGCAAAGGGCCAACACGCAACTAGGTCGTACCTACCACGAGATGTATCTAAAATCCCTGGACGAACATCAGTCGGTGCAGAATGCCAAAAAGTACTTCAAGTCTGCTATGAAGCTTGCACAGGCTATCAAAGAGAACCCACCTTCTAATAAATCTTTTTTTATCAAAGAATATATTGACGCGCATAACAATGTAGGATTGCTTGAACTTGATCGTGATAACCTGGAAGAGGCTGAGAAAATCTTGACTAGAGCATTAGTTATTTGCGACCAAGAAGAGGTGAATGAATATGATGATGGGCGCACCAGGCTTCATCACAATCTTGGAAAGATTTACACCGAGCTGAGGATGTGGGAAAATGCTCGGGAACACATTGAGAAGGACATTCTGATATGTAAACAAATTGGGCATTGTGAAGGAGAGGCAAAGGGGTATATCAATCTTGGCGAGTTGCATAACCGTGTTCAGAAATATCAGGAGGCAATCCTTTGCTACCAAAAAGCACTTGATCTAGCAAATTCAATGGAAGATGAGGATGCCTTGGTACGTCAAATTGAAGACAATATCAAAACTGTAAAAGAATTAGGTAAAGTAATGGATGACATGAAGAGAGAAGAGCAAAATCTCAAGAAGCTAACAAGAGACATGGCCATAGCAAGAGGCACAACACATAAGAGGAATTGTCAGCTCAAGCAAATTGCTTCTCTTGATGTTCTTATTAACAAATCAGAGACTATCTCTGCCTGGCCAAAGGTCCTTGAATTTGCGAAAAAGAAGAAGAAAGTAGCAACTGAAATTTGTGATAAAGAAAAGATTGCTGATTCACTTTTGAGCATTGGAGAATCCTACTTGAAGCTAAGGAAGTTCCAGAAAGCCCTTAAATGGTGCATGAAGAGTTTGAAAATTTACAAATCACTTGGAAACTTGGAGGGGCAGGCGTTGACACAAATTAACATAGGTCATGTTTTGGACAGTGAAGGTTATTATGAAGAAGCACTCAATGCATTTACAGATAGCTACAGGCTTGCTGTTGAGGGTAAACTTTCTTATGTACAGCGTACTGCACTAGAAAATATGCACTATAGCAACTTGAACAGATTTGATGATGTTGAAGAGACCAGGAGATTGCAACTTGAAATTGACAAATTGGAGCAAAACAATGAAGGGCTTGAAACAGAAATTACGGCAGAGGATCGCTGCTCTGAATCTAGTACAGAAGGGAGTGGTGATTTCTCAGATAATATGCCTAATGCATGCTGTCCAGAAGAGATAAGAAACTCCCAATCTAGCAAATCACAATCTTTAAACCAAAAGGAAGAGTTGAATGATGATGTACCTTTGATTTCTCTCATCCGATCTACCAAAGTGTCGTCTAAGATGAAATCAGCTCATTTCGAAAACAATGAGCTTGGAGTGGTGGGCCGTAAACGTATTCGTTTAGTCCTGTCTGATGATGAAGACGAAATGTCTGATGAGGCAGAAAGCCCAAAAGTCAGGCATAATAAAATCCCATTAAATGTCGCTACATCTAGTGAAGTTAAGACTAGAAGTAATACAGCCAGTCCTACTACTAAATTTCAGGATGTCTCAGCCTTCACCTCAAATTGCGCCACAAGATCTGATCCTCCTGTTAATACTGAAGAAAGCTCTAGTTCATGCAAATCCAGGACTCTTAATATGGCCACTCCAGAAGGCAAATCTTTTAGAGCTTCAAGTTCTGGTGAAGGTTTTAATGCTAGTGGCTCTAAATGTGATGTCAGTGTCTCTGGAAATATATTGCATAAAAATGACGCTGCACAATTTATGTTGCTTACTTCGGATGATAAACATAATCAATGTATAACTTTTGAAATCGATGAAGACATAATACAGGGCTCATTCATGGCTTCTGATAATTTAAGCATTGAGTCAGTGAAGGTTCAACTGGCATGCTTATACTACTTGCAACTTCCTATGGAGAGAAAACTCGAGGGGCTAGTTCCAATAATTCAGAATGTAAAATGTGGTGAAAAAGCTATTGAATCCCTCGAAACAATCAAAACATTTCGGGGGCATATGGGAAAAATTGTGGTTGAAGCTGTTATTGATGGATGGGTCCAAAAGCGCTTGATAAAACTGTACACCGATCACTGCTACAAGCTATCTGAGACACCCAACATGAAGTTGCTAAAGAAACTATATGATCTGGAGGTTTCAGACGATGAAGTTACTGTTTCTGAATGTGAATTGCAAGATTTATCAATAACTCCACTGTTGAATGCCCTCCATGCCCATAAAACATTTGCAATGCTAGACCTTTCCCACAACTTGTTAGGAAATGGTACTATGGAGAAACTCCAGCAAGTACTCACATCATCAGGCCAAAAATATGGTGGCTTAACATTGGATCTGCATTGCAACAGCTTTGGTCCAACTGCTTTGTTCCAGATTTGTGAGTGCCCTCTGCTATTTACCCGATTGGAAGTGCTTAATATCTCAGGAAATCGTCTCACTGATGCATGCGCATCTTACCTTTCAACTATCTTGGAGAATTGCAAGGCCCTTTGCAGTTTAAGTATAGAGCGATGCTCCATCACATCTAGAACTATTCAAAAGGTTGCTGATGCGTTGAGTGCTGAATCTGTCCTTGAGCAACTTTGTATAGGATATAACAAGCCTATTTCTGGAAGTGCCATTACTAATTTACTCGTCAAGCTTGGCACTCTGAAAAGATTTTCAAAATTAAATATGAACGGCTTGAAGCTGAGCAAGCCTGTGGTTGATAGTCTTTGCCAGCTTGCTAAACGCTTGTCCTTGTCAGTACTAATGCTTGGGGAAACTGGTATCGGACTTGACGGAGCATTGTTAGTAACCGAGTCATTGTTCCATGAAATTGAAGAGTTTGTTAAACTTGACTTATCATATTGCGGAGTGACATATAATTATGCTGTCAAACTAAGCACTAATTCTTCTATGGTTTGTGGCATTCTTGAGCTGAGCCTTTCAGGAAATCCTATTATGCAAGAGGGTAGCAATGCATTATCATCAATGCTTTTGAATTCTCAATGTTGTCTGAAAGTTTTGGTCCTTCAAAAGTGCCAGCTTGGGATCTCTGGCATTCTGCAAATTATCCAGGCATTATCAGGTAATGCTTTTCTTGAAGAGCTCAATCTTGCTGAAAATGCGGATATTGGTAAAGAGTACTCTGCACCATATGACGTGGCAGAAAAAGGGGGTGTCGGACTCTCACAGCCAAACCTCAATATGCTTGAATCTTCTCCATTGGTATCTGTGCTCAAAGATGTTAGCCAGCTTGAAGTTGCTGATAGTGAAGATGATGAAGTTGGGGTTGAAGCTGCTGCATCTGCACGCAACGAGAACTGTTCAAACTCATTTCAAAGAAATTCCTCATCCACTGAGTGTCAGTTTCTTGAAGAGTTTTCAACTGCCATTGATATGGCAAAGAATCTGCGGTTCCTGGACCTTAGCAAAAATGGTTTCTCCACACAATGTGCAGAGATACTTTATTCGTCATGGTCAAGATCAAGATCTGTTTCAGCTCGGAGGCATATCAAGGACAAGACCATCCACTTCTTTGTGGAAGGAATGATGTGTTGTGTGAAACCCTGCTGCAGGAAGGACTAA

Protein Analysis

1313

Amino Acids

146.93

Weight (kDa)

5.56

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 166 - 232 1.1e-06 Tetratricopeptide repeat
TPR_12 PF13424 209 - 279 4.3e-09 Tetratricopeptide repeat
TPR_1 PF00515 248 - 277 7.8e-06 Tetratricopeptide repeat
TPR_12 PF13424 379 - 449 1.6e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18730
fragaria_vesca FvH4_1g23980 FvH4_1g23980 FvH4_1g23980
malus_domestica MD00G1172700.v1.1 MD09G1255500.v1.1 MD17G1248600.v1.1
prunus_persica Prupe.3G147500_v2.0.a1 Prupe.3G147500_v2.0.a1
pyrus_communis pycom09g17130
rosa_chinensis RchiOBHm_Chr2g0120111
rosa_laevigata RLG00000018521
rosa_multiflora Rmu_sc0010863.1_g000001
rosa_roxburghii Rroxscaffold_2G00123720
rosa_rugosa Rorug02G0226800
rosa_samantha Rh2AG283400 Rh2BG294700 Rh2DG308400
rosa_wichuraiana Rw2G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2821
Acc36I ACCTGC 1 cut(s) 141
AccII CGCG 2 cut(s) 53, 500
AciI CCGC 3 cut(s) 3225, 3482, 3769
AclWI GGATC 4 cut(s) 1518, 1934, 2712, 3413
AcoI YGGCCR 3 cut(s) 966, 1064, 1998
AcsI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3355, 3700
AcuI CTGAAG 1 cut(s) 1977
AcyI GRCGYC 2 cut(s) 38, 258
AdeI CACNNNGTG 2 cut(s) 3717, 3809
AfeI AGCGCT 1 cut(s) 2423
AfiI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2981, 3930
AhdI GACNNNNNGTC 1 cut(s) 1783
AjiI CACGTC 1 cut(s) 3517
AjnI CCWGG 8 cut(s) 375, 540, 623, 1061, 1436, 1982, 3429, 3774
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
Alw21I GWGCWC 2 cut(s) 3465, 3594
Alw26I GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
AlwI GGATC 4 cut(s) 1518, 1934, 2712, 3413
Ama87I CYCGRG 3 cut(s) 242, 681, 2285
Aor51HI AGCGCT 1 cut(s) 2423
ApeKI GCWGC 6 cut(s) 319, 1515, 2111, 3653, 3656, 3927
ApoI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3355, 3700
AspLEI GCGC 6 cut(s) 53, 502, 621, 1928, 2424, 2822
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 6 cut(s) 236, 610, 1556, 1906, 2051, 2336
AsuII TTCGAA 2 cut(s) 252, 1713
AvaI CYCGRG 3 cut(s) 242, 681, 2285
AvaII GGWCC 5 cut(s) 1072, 2413, 2726, 3382, 3778
BaeGI GKGCMC 1 cut(s) 2759
BalI TGGCCA 3 cut(s) 968, 1066, 2000
BanII GRGCYC 2 cut(s) 2195, 3465
BarI GAAGNNNNNNTAC 6 cut(s) 26, 58, 2169, 2201, 2977, 3009
BauI CACGAG 3 cut(s) 71, 263, 356
BbsI GAAGAC 4 cut(s) 261, 882, 1782, 2184
Bbv12I GWGCWC 2 cut(s) 3465, 3594
BbvCI CCTCAGC 2 cut(s) 294, 662
BbvI GCAGC 6 cut(s) 331, 1502, 2098, 3640, 3643, 3914
BccI CCATC 7 cut(s) 68, 174, 608, 2399, 2403, 2897, 3890
BceAI ACGGC 3 cut(s) 1518, 1725, 3067
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 8 cut(s) 377, 542, 625, 1063, 1438, 1984, 3431, 3776
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BfmI CTRYAG 4 cut(s) 134, 1336, 1399, 3928
BfoI RGCGCY 1 cut(s) 2425
BfuAI ACCTGC 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 89
BglII AGATCT 2 cut(s) 1934, 3845
BisI GCNGC 6 cut(s) 320, 1516, 2112, 3654, 3657, 3928
BlpI GCTNAGC 2 cut(s) 3062, 3293
BlsI GCNGC 6 cut(s) 321, 1517, 2113, 3655, 3658, 3929
BmcAI AGTACT 5 cut(s) 182, 413, 2664, 3120, 3500
Bme1390I CCNGG 9 cut(s) 85, 377, 542, 625, 1063, 1438, 1984, 3431, 3776
Bme18I GGWCC 5 cut(s) 1072, 2413, 2726, 3382, 3778
BmeRI GACNNNNNGTC 1 cut(s) 1783
BmeT110I CYCGRG 3 cut(s) 242, 681, 2285
BmgBI CACGTC 1 cut(s) 3517
BmiI GGNNCC 2 cut(s) 2414, 3773
BmrFI CCNGG 9 cut(s) 85, 377, 542, 625, 1063, 1438, 1984, 3431, 3776
BmsI GCATC 7 cut(s) 276, 844, 2803, 2831, 2873, 2911, 3668
BpiI GAAGAC 4 cut(s) 261, 882, 1782, 2184
BplI GAGNNNNNCTC 4 cut(s) 1040, 1072, 2870, 2902
BpmI CTGGAG 3 cut(s) 1989, 2525, 2639
Bpu10I CCTNAGC 4 cut(s) 294, 662, 1175, 3782
Bpu1102I GCTNAGC 2 cut(s) 3062, 3293
Bpu14I TTCGAA 2 cut(s) 252, 1713
BpuEI CTTGAG 5 cut(s) 128, 929, 992, 2966, 3308
BpuMI CCSGG 1 cut(s) 85
Bsa29I ATCGAT 2 cut(s) 159, 2172
BsaBI GATNNNNATC 2 cut(s) 165, 3844
BsaHI GRCGYC 2 cut(s) 38, 258
BsaI GGTCTC 1 cut(s) 1427
BsaJI CCNNGG 3 cut(s) 220, 375, 858
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2981, 3930
Bse1I ACTGG 3 cut(s) 1878, 2247, 3142
Bse3DI GCAATG 3 cut(s) 2610, 2710, 3337
Bse8I GATNNNNATC 2 cut(s) 165, 3844
BseBI CCWGG 8 cut(s) 377, 542, 625, 1063, 1438, 1984, 3431, 3776
BseCI ATCGAT 2 cut(s) 159, 2172
BseDI CCNNGG 3 cut(s) 220, 375, 858
BseGI GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3707, 3882
BseJI GATNNNNATC 2 cut(s) 165, 3844
BseLI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2981, 3930
BseMI GCAATG 3 cut(s) 2610, 2710, 3337
BseNI ACTGG 3 cut(s) 1878, 2247, 3142
BseRI GAGGAG 3 cut(s) 89, 92, 311
BseSI GKGCMC 1 cut(s) 2759
BseXI GCAGC 6 cut(s) 331, 1502, 2098, 3640, 3643, 3914
BsgI GTGCAG 7 cut(s) 157, 416, 1365, 2097, 3489, 3648, 3831
Bsh1236I CGCG 2 cut(s) 53, 500
BshVI ATCGAT 2 cut(s) 159, 2172
BsiHKAI GWGCWC 2 cut(s) 3465, 3594
BsiHKCI CYCGRG 3 cut(s) 242, 681, 2285
BsiSI CCGG 2 cut(s) 46, 85
BslFI GGGAC 2 cut(s) 172, 202
BslI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2981, 3930
BsmAI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BsmBI CGTCTC 2 cut(s) 245, 2810
BsmFI GGGAC 2 cut(s) 172, 202
BsmI GAATGC 4 cut(s) 406, 2581, 3282, 3414
Bso31I GGTCTC 1 cut(s) 1427
BsoBI CYCGRG 3 cut(s) 242, 681, 2285
Bsp119I TTCGAA 2 cut(s) 252, 1713
Bsp1286I GDGCHC 4 cut(s) 2195, 2759, 3465, 3594
Bsp1407I TGTACA 2 cut(s) 1369, 2436
Bsp1720I GCTNAGC 2 cut(s) 3062, 3293
BspACI CCGC 3 cut(s) 3225, 3482, 3769
BspDI ATCGAT 2 cut(s) 159, 2172
BspFNI CGCG 2 cut(s) 53, 500
BspLI GGNNCC 2 cut(s) 2414, 3773
BspMAI CTGCAG 2 cut(s) 138, 3932
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 4 cut(s) 1518, 1934, 2712, 3413
BspQI GCTCTTC 2 cut(s) 927, 3453
BspT104I TTCGAA 2 cut(s) 252, 1713
BspTNI GGTCTC 1 cut(s) 1427
BsrDI GCAATG 3 cut(s) 2610, 2710, 3337
BsrGI TGTACA 2 cut(s) 1369, 2436
BsrI ACTGG 3 cut(s) 1878, 2247, 3142
BssECI CCNNGG 3 cut(s) 220, 375, 858
BssNI GRCGYC 2 cut(s) 38, 258
BssSI CACGAG 3 cut(s) 71, 263, 356
BssT1I CCWWGG 1 cut(s) 858
Bst2BI CACGAG 3 cut(s) 71, 263, 356
Bst2UI CCWGG 8 cut(s) 377, 542, 625, 1063, 1438, 1984, 3431, 3776
Bst4CI ACNGT 7 cut(s) 779, 892, 1292, 2436, 2530, 2571, 3680
BstACI GRCGYC 2 cut(s) 38, 258
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstAUI TGTACA 2 cut(s) 1369, 2436
BstBI TTCGAA 2 cut(s) 252, 1713
BstENI CCTNNNNNAGG 3 cut(s) 224, 545, 3928
BstF5I GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3707, 3882
BstFNI CGCG 2 cut(s) 53, 500
BstH2I RGCGCY 1 cut(s) 2425
BstHHI GCGC 6 cut(s) 53, 502, 621, 1928, 2424, 2822
BstMAI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BstMWI GCNNNNNNNGC 9 cut(s) 89, 299, 625, 1319, 2585, 2930, 3067, 3479, 3662
BstNI CCWGG 8 cut(s) 377, 542, 625, 1063, 1438, 1984, 3431, 3776
BstNSI RCATGY 3 cut(s) 1575, 2250, 2820
BstSCI CCNGG 9 cut(s) 83, 375, 540, 623, 1061, 1436, 1982, 3429, 3774
BstSFI CTRYAG 4 cut(s) 134, 1336, 1399, 3928
BstSLI GKGCMC 1 cut(s) 2759
BstUI CGCG 2 cut(s) 53, 500
BstV1I GCAGC 6 cut(s) 331, 1502, 2098, 3640, 3643, 3914
BstV2I GAAGAC 4 cut(s) 261, 882, 1782, 2184
BstX2I RGATCY 4 cut(s) 1934, 2704, 3405, 3845
BstXI CCANNNNNNTGG 2 cut(s) 2687, 3754
BstYI RGATCY 4 cut(s) 1934, 2704, 3405, 3845
Bsu15I ATCGAT 2 cut(s) 159, 2172
BsuTUI ATCGAT 2 cut(s) 159, 2172
BtgZI GCGATG 1 cut(s) 2896
BtrI CACGTC 1 cut(s) 3517
BtsCI GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3707, 3882
BtsI GCAGTG 1 cut(s) 2446
BtsIMutI CAGTG 7 cut(s) 1297, 2083, 2235, 2446, 2567, 2808, 3711
BveI ACCTGC 1 cut(s) 141
CfoI GCGC 6 cut(s) 53, 502, 621, 1928, 2424, 2822
ClaI ATCGAT 2 cut(s) 159, 2172
CseI GACGC 4 cut(s) 27, 266, 506, 2117
DraI TTTAAA 1 cut(s) 1623
DraIII CACNNNGTG 2 cut(s) 3717, 3809
DriI GACNNNNNGTC 1 cut(s) 1783
EaeI YGGCCR 3 cut(s) 966, 1064, 1998
Eam1105I GACNNNNNGTC 1 cut(s) 1783
Ecl136II GAGCTC 1 cut(s) 3463
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 2 cut(s) 2195, 3465
Eco31I GGTCTC 1 cut(s) 1427
Eco47I GGWCC 5 cut(s) 1072, 2413, 2726, 3382, 3778
Eco47III AGCGCT 1 cut(s) 2423
Eco53kI GAGCTC 1 cut(s) 3463
Eco57I CTGAAG 1 cut(s) 1977
Eco88I CYCGRG 3 cut(s) 242, 681, 2285
EcoICRI GAGCTC 1 cut(s) 3463
EcoNI CCTNNNNNAGG 3 cut(s) 224, 545, 3928
EcoO109I RGGNCCY 3 cut(s) 139, 1072, 2857
EcoRI GAATTC 1 cut(s) 3355
EcoRII CCWGG 8 cut(s) 375, 540, 623, 1061, 1436, 1982, 3429, 3774
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 4 cut(s) 1324, 1573, 2818, 3337
EcoT38I GRGCYC 2 cut(s) 2195, 3465
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 2 cut(s) 245, 2810
FaqI GGGAC 2 cut(s) 172, 202
FauNDI CATATG 2 cut(s) 2373, 3511
Fnu4HI GCNGC 6 cut(s) 320, 1516, 2112, 3654, 3657, 3928
FokI GGATG 9 cut(s) 679, 866, 929, 1655, 1913, 2150, 2421, 3694, 3869
FriOI GRGCYC 2 cut(s) 2195, 3465
Fsp4HI GCNGC 6 cut(s) 320, 1516, 2112, 3654, 3657, 3928
FspAI RTGCGCAY 1 cut(s) 2821
FspI TGCGCA 1 cut(s) 2821
GlaI GCGC 6 cut(s) 52, 501, 620, 1927, 2423, 2821
GluI GCNGC 6 cut(s) 320, 1516, 2112, 3654, 3657, 3928
GsuI CTGGAG 3 cut(s) 1989, 2525, 2639
HaeII RGCGCY 1 cut(s) 2425
HapII CCGG 2 cut(s) 46, 85
HgaI GACGC 4 cut(s) 27, 266, 506, 2117
HhaI GCGC 6 cut(s) 53, 502, 621, 1928, 2424, 2822
Hin1I GRCGYC 2 cut(s) 38, 258
Hin6I GCGC 6 cut(s) 51, 500, 619, 1926, 2422, 2820
HinP1I GCGC 6 cut(s) 51, 500, 619, 1926, 2422, 2820
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 2 cut(s) 431, 3011
HpaII CCGG 2 cut(s) 46, 85
HphI GGTGA 6 cut(s) 236, 610, 1556, 1906, 2051, 2336
Hpy166II GTNNAC 5 cut(s) 716, 1260, 1358, 1745, 2439
Hpy8I GTNNAC 5 cut(s) 716, 1260, 1358, 1745, 2439
HpyCH4III ACNGT 7 cut(s) 779, 892, 1292, 2436, 2530, 2571, 3680
HpyCH4IV ACGT 4 cut(s) 96, 865, 1747, 3516
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 299, 625, 1319, 2585, 2930, 3067, 3479, 3662
HpySE526I ACGT 4 cut(s) 96, 865, 1747, 3516
Hsp92I GRCGYC 2 cut(s) 38, 258
HspAI GCGC 6 cut(s) 51, 500, 619, 1926, 2422, 2820
LguI GCTCTTC 2 cut(s) 927, 3453
LmnI GCTCC 4 cut(s) 48, 1468, 2891, 3155
Lsp1109I GCAGC 6 cut(s) 331, 1502, 2098, 3640, 3643, 3914
LweI GCATC 7 cut(s) 276, 844, 2803, 2831, 2873, 2911, 3668
MaeII ACGT 4 cut(s) 96, 865, 1747, 3516
MaeIII GTNAC 3 cut(s) 2524, 3166, 3229
MflI RGATCY 4 cut(s) 1934, 2704, 3405, 3845
MhlI GDGCHC 4 cut(s) 2195, 2759, 3465, 3594
MlsI TGGCCA 3 cut(s) 968, 1066, 2000
MluNI TGGCCA 3 cut(s) 968, 1066, 2000
MlyI GAGTC 4 cut(s) 1981, 2234, 3182, 3534
MmeI TCCRAC 2 cut(s) 2753, 3517
Mox20I TGGCCA 3 cut(s) 968, 1066, 2000
Mph1103I ATGCAT 4 cut(s) 1324, 1573, 2818, 3337
MscI TGGCCA 3 cut(s) 968, 1066, 2000
MslI CAYNNNNRTG 4 cut(s) 507, 777, 2103, 3752
Msp20I TGGCCA 3 cut(s) 968, 1066, 2000
MspI CCGG 2 cut(s) 46, 85
MspR9I CCNGG 9 cut(s) 85, 377, 542, 625, 1063, 1438, 1984, 3431, 3776
Mva1269I GAATGC 4 cut(s) 406, 2581, 3282, 3414
MvaI CCWGG 8 cut(s) 377, 542, 625, 1063, 1438, 1984, 3431, 3776
MvnI CGCG 2 cut(s) 53, 500
MwoI GCNNNNNNNGC 9 cut(s) 89, 299, 625, 1319, 2585, 2930, 3067, 3479, 3662
NciI CCSGG 1 cut(s) 85
NdeI CATATG 2 cut(s) 2373, 3511
NlaIV GGNNCC 2 cut(s) 2414, 3773
NmuCI GTSAC 1 cut(s) 3229
NsbI TGCGCA 1 cut(s) 2821
NsiI ATGCAT 4 cut(s) 1324, 1573, 2818, 3337
NspI RCATGY 3 cut(s) 1575, 2250, 2820
NspV TTCGAA 2 cut(s) 252, 1713
PaeI GCATGC 3 cut(s) 1575, 2250, 2820
PaeR7I CTCGAG 2 cut(s) 242, 2285
PciSI GCTCTTC 2 cut(s) 927, 3453
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 249
PctI GAATGC 4 cut(s) 406, 2581, 3282, 3414
PfeI GAWTC 7 cut(s) 1139, 1160, 1523, 2339, 2936, 3569, 3763
PfoI TCCNGGA 2 cut(s) 1982, 3774
PkrI GCNGC 6 cut(s) 321, 1517, 2113, 3655, 3658, 3929
PleI GAGTC 4 cut(s) 1981, 2233, 3181, 3534
PpsI GAGTC 4 cut(s) 1981, 2233, 3181, 3534
PpuMI RGGWCCY 1 cut(s) 1072
Psp124BI GAGCTC 1 cut(s) 3465
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 8 cut(s) 375, 540, 623, 1061, 1436, 1982, 3429, 3774
PspGI CCWGG 8 cut(s) 375, 540, 623, 1061, 1436, 1982, 3429, 3774
PspN4I GGNNCC 2 cut(s) 2414, 3773
PspPPI RGGWCCY 1 cut(s) 1072
PspXI VCTCGAGB 1 cut(s) 2285
PstI CTGCAG 2 cut(s) 138, 3932
PsuI RGATCY 4 cut(s) 1934, 2704, 3405, 3845
RseI CAYNNNNRTG 4 cut(s) 507, 777, 2103, 3752
SacI GAGCTC 1 cut(s) 3465
SapI GCTCTTC 2 cut(s) 927, 3453
SatI GCNGC 6 cut(s) 320, 1516, 2112, 3654, 3657, 3928
SbfI CCTGCAGG 1 cut(s) 138
ScaI AGTACT 5 cut(s) 182, 413, 2664, 3120, 3500
SchI GAGTC 4 cut(s) 1981, 2234, 3182, 3534
ScrFI CCNGG 9 cut(s) 85, 377, 542, 625, 1063, 1438, 1984, 3431, 3776
SdaI CCTGCAGG 1 cut(s) 138
SduI GDGCHC 4 cut(s) 2195, 2759, 3465, 3594
SfaNI GCATC 7 cut(s) 276, 844, 2803, 2831, 2873, 2911, 3668
SfcI CTRYAG 4 cut(s) 134, 1336, 1399, 3928
SfiI GGCCNNNNNGGCC 1 cut(s) 89
Sfr274I CTCGAG 2 cut(s) 242, 2285
SfuI TTCGAA 2 cut(s) 252, 1713
SinI GGWCC 5 cut(s) 1072, 2413, 2726, 3382, 3778
SlaI CTCGAG 2 cut(s) 242, 2285
SmiMI CAYNNNNRTG 4 cut(s) 507, 777, 2103, 3752
SmlI CTYRAG 7 cut(s) 143, 242, 944, 1007, 2285, 2945, 3287
SmoI CTYRAG 7 cut(s) 143, 242, 944, 1007, 2285, 2945, 3287
SphI GCATGC 3 cut(s) 1575, 2250, 2820
Sse8387I CCTGCAGG 1 cut(s) 138
SsiI CCGC 3 cut(s) 3225, 3482, 3769
SstI GAGCTC 1 cut(s) 3465
StyD4I CCNGG 9 cut(s) 83, 375, 540, 623, 1061, 1436, 1982, 3429, 3774
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 7 cut(s) 779, 892, 1292, 2436, 2530, 2571, 3680
TaiI ACGT 4 cut(s) 99, 868, 1750, 3519
TaqI TCGA 8 cut(s) 153, 159, 243, 252, 1713, 2172, 2286, 2346
TatI WGTACW 8 cut(s) 180, 411, 1369, 1529, 2436, 2662, 3118, 3498
TfiI GAWTC 7 cut(s) 1139, 1160, 1523, 2339, 2936, 3569, 3763
TscAI CASTG 7 cut(s) 1297, 2083, 2235, 2453, 2574, 2815, 3718
TseFI GTSAC 1 cut(s) 3229
TseI GCWGC 6 cut(s) 319, 1515, 2111, 3653, 3656, 3927
Tsp45I GTSAC 1 cut(s) 3229
TspGWI ACGGA 2 cut(s) 227, 3168
TspRI CASTG 7 cut(s) 1297, 2083, 2235, 2453, 2574, 2815, 3718
VpaK11BI GGWCC 5 cut(s) 1072, 2413, 2726, 3382, 3778
XagI CCTNNNNNAGG 3 cut(s) 224, 545, 3928
XapI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3355, 3700
XbaI TCTAGA 1 cut(s) 2897
XceI RCATGY 3 cut(s) 1575, 2250, 2820
XcmI CCANNNNNNNNNTGG 1 cut(s) 2687
XhoI CTCGAG 2 cut(s) 242, 2285
ZrmI AGTACT 5 cut(s) 182, 413, 2664, 3120, 3500
Zsp2I ATGCAT 4 cut(s) 1324, 1573, 2818, 3337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.