RLG00000018521

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012067
Physical Location & Seq
Forward (+)
31276208 .. 31287816
11609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018521

Sequence Viewer

Length: 3789 bp
ATGGCAAGGGAGGAGGCGAAGCTGAGTGAGGCGAAGGCGTCTTACCGGAGCGCGAAGGCCATCGGCAACCACGAGGAGGAGGCCCGGTGGGCCAACGTCATCGGCAACTTGTTGAAGGACAGAGGCGAGTACCTGCAGGCCCTAAAGTGGTTCGACATAGATTACCATCTCTGCACCAAGTACTTGTCCCCCAAGCACTCCCTTGCCACCTGTCAGTCCCTCGGTGAGGTCTATCTCCGTCTCGAGCGTTTCGAAGACGCCCTTGTGTATCAGAAAAAACATTTGATGCTTGCTGAGGAGGCCAATGACCTTGTTGAGCAGCAAAGGGCCAATACGCAACTAGGTCGTACCTACCACGAGATGTATCTAAAATCCCTGGACGAACATCAGTCGGTGCAGAATGCCAAAAAGTACTTCAAGTCTGCTATGAAGCTTGCACAGGCTATCAAAGAGAACCCACCTTCTAATAAATCTTTCTTTATCAAAGAATATATTGACGCGCATAACAATGTAGGATTGCTTGAACTTGATCGTGATAACCTGGAAGAGGCTGAGAAAATCTTGACTAAAGCATTAGTTATTTGCGACCAAGAAGAGGTGAATGAATATGATGATGGGCGCACCAGGCTTCATCACAATCTTGGAAAGATTTACACCGAGCTGAGGATGTGGGAAAATGCTCGGGAACACATTGAGAAGGACATTCTGATATGTAAACAAATTGGGCATTGTGAAGGAGAGGCAAAGGGCTATATCAATCTTGGCGAGTTGCATAACCGTGTTCAGAAATATCAGGAGGCAATCCTTTGCTACCAAAAAGCACTTGATCTAGCAAATTCAATGGAAGATGAGGATGCCTTGGTACGTCAAATTGAAGACAATATCAAAACTGTCAAAGAATTAGGTAAAGTAATGGATGACATGAAGAGAGAAGAGCAAAATCTCAAGAAGCTAACAAGAGACATGGCCATAGCAAGAGGCACAACACATAAGAGGAATTGTCAGCTCAAGCAAATTGCTTCTCTTGATGTTCTTATTAACAAATCAGAGACTATCTCTGCCTGGCCAAAGGTCCTTGAATTTGCAAAAAAGAAGAAGAAAGTAGCAACTGAAATTTGTGATAAAGAAAAGATTGCTGATTCACTTTTGAGCATTGGAGAATCCTACTTGAAGCTAAGGAAGTTCCAGAAAGCCCTTAAATGGTGCATGAAGAGTTTGAAAATTTACAAATCACTTGGAAACTTGGAGGGACAGGCGTTGACACAAATTAACATAGGTCATGTTTTGGACAGTGAAGGTTATTATGAAGAAGCACTCGGTGCATTTACAGATAGCTACAGGCTTGCTGTTGAGGGTAAGCTTTCGTATGTACAGCGTACTGCACTAGAAAATATGCACTATAGCAACTTGAACAGATTTGATGATGTTGAAGAGACCAGGAGATTGCAACTTGAAATTGACAAATTGGAGCAAAACAATGAAGGGCTTGAAACAGAAATTACGGCAGAGGATCGCTGCTCTGAATCTAGTACAGAAGGGAGTGGTCATTTCTCAGATAATATGCCTAATGCATGCTGTTCAGAAGAGATAAGAAACTCCCAATCTAGCAAATCACAATCTTTAAACCAAAAGGAAGAGTTGAATGATGATCTACCTTTGATTTCTCTCATCCGATCTACCAAAGTGTCGTCTAAGATGAAATCAGCTCATTTCGAAAACAATGAGCATGGAGTGGTGGGCCGTAAACGCATTCGTTTAGTCCTGTCTGATGATGACGATCAACTGTCTGATGAGGCAGAAAGCCCAAAAGTCAGGCATAATAAAATCCCATTAAATGTCGCTAAATCTAGTAAAGTTAAGACTAGAAGTAATACAGCCAGTCCTACTACTAAATTTCAGGATGTCTCAGCCTTTACCTCAAATTGTGCCACAAGATCTGATCCTCCTGTTAATACGGAGGAAAGCTCTAGTTCATGCAAATCTAGGACTCTTAATATGGCCGCTCCAGAAGGCAAATCTTTCAGAGCTTCAAGTTCTGGTGAAGGTTTTAATGCTAGTGGCTCTAAATGTGATGTCAGTGTCTCTGGAAATATATTGCATAAAAATGACGCTGCACAATTTATGTTGCTTACTTCGGATGATAAACATAATCAATGTATAACTTTTGAAATCAATGAAGACATAATACAGGACTCATTCATGGCTTCTGATAATTTAAGCATTGAGTCAGTGAAGGTTCAACTGGCATGCTTATACTACTTGCAACTTCCTATGGAGAGAAAACTCGAGGGGCTACTTCCAATAATTCAGAATGTAAAATGTGGTGAAAAAGCTATTGAATCCCTTGAAACAATCAAAACATTTCAAGGGCATATGGGAAAAATTGTGGTTGAAGCTGTCATTGATGGATGGGTCCAAAAGCGCTTGATAAAACTATACACCGATTACTGCTACAAGGTATCTGAGACTCCCAACATGAAGGTGCTAAAGAAACTATATGATCTGGAGGTTTCAGACGATGAAGTTACTGTTTCTGAATGTGAATTGCAAGATTTATCAATAACTCCACTGTTGAATGCCCTCCAAGCCCATAAAACATTTGCAATGCTAGACCTTTCCCACAATTTGTTAGGAAATGGTACTATGGAGAAACTTCAGCAAGTACTCACATCATCAGGCCAAAAATATGGTGGCTTAACGTTGGATCTGCATTGCAACAGCTTTGGTCCAACTGCTTTGTTCCAGATTTGTGAGTGCCCTCTGCTATTTACTCGATTGGAAGTGCTTAATATCTCAGGAAATCGTCTCACTGATGCATGCCCTATTTCTGGAAGTGCCATTACTAATTTACTCGTCAAGCTTGGCACTCTGAAAAGATTTTCAAAATTAAATATGAACGGCTTGAAGCTGAGCAAGCCTGTGGTTGATAGTCTTTGCCAGCTTGCTAAACGCTTGTCCTTGTCAGTACTAATGCTTGGGGAAACTGGTATCGGACTTGACGGAGCATTGTTAGTAACTGAGTCATTGTTCCATGAAATTGAAGAGTTTGTTAAACTTGACTTATCATATTGCGGAGTGACATATAATTATGCTGTCAAACTAAGCACTAATTCTTCTATGGTTTGTGGCATTCTTGAGCTGAGCCTTTCAGGAAATCCTATTATGCAAGAGGGTAGCAATGCATTATCATCAATGCTTTTGAATTCTCAATGTTGTCTGAAAGTTTTGGTCCTTCAAAAGTGCCAGCTTGGGATCTCTGGCGTTCTGCAAATTATCCAGGCATTATCAGGTAATGCTTTTCTTGAAGAGCTCAATCTTGCTGAAAATGCGGATATTGGTAAAGAGTTCTCTGCACCATATGGCGTGGCAGAAAAAGGGAGTGTTGGACTCTCGCAGCCAAACCTCAATATGCTTGAATCTTCTCCATTGGTATCTGCGCTCCAAGATGTTAGCCAGCTTGAAGTTGCTGATAGTGAAGATGATGAAGTTGGGGTTGAAGCTGCTGCATCTGCACTCAACGAGAGCTGTTCAAGCTCATTTCAAAGAAATTCCTCATCCACTGAGTGCCAGTTTCTTGAAGAGTTTTCAACTGCCATTGATATGGCAAAGAATCTGCGGTTCCTGGACCTTAGCAAAAATGGTTTCTCCACACAATGTGCAGAGATACTGTATTCGTCATGGTCAAGATCAAGATCTGTTTCAGCTCGGAGGCATATCAAGGACAAGACCATCCACTTCTTTGTGGAAGGAATGATGTGTTGTGTGAAACCCTGCTGCAGGAAGGACTAA

Protein Analysis

1263

Amino Acids

141.19

Weight (kDa)

5.63

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 166 - 232 1e-06 Tetratricopeptide repeat
TPR_12 PF13424 209 - 279 4.1e-09 Tetratricopeptide repeat
TPR_1 PF00515 248 - 277 7.5e-06 Tetratricopeptide repeat
TPR_12 PF13424 379 - 449 8.7e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012067)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18730
fragaria_vesca FvH4_1g23980 FvH4_1g23980 FvH4_1g23980
malus_domestica MD00G1172700.v1.1 MD09G1255500.v1.1 MD17G1248600.v1.1
prunus_persica Prupe.3G147500_v2.0.a1 Prupe.3G147500_v2.0.a1
pyrus_communis pycom09g17130
rosa_chinensis RchiOBHm_Chr2g0120111
rosa_laevigata RLG00000018521
rosa_multiflora Rmu_sc0010863.1_g000001
rosa_roxburghii Rroxscaffold_2G00123720
rosa_rugosa Rorug02G0226800
rosa_samantha Rh2AG283400 Rh2BG294700 Rh2DG308400
rosa_wichuraiana Rw2G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 2003
AccII CGCG 2 cut(s) 53, 500
AciI CCGC 4 cut(s) 2001, 3072, 3329, 3616
AclI AACGTT 1 cut(s) 2699
AclWI GGATC 4 cut(s) 1518, 1934, 2712, 3260
AcoI YGGCCR 3 cut(s) 966, 1064, 1998
AcsI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3202, 3547
AcuI CTGAAG 1 cut(s) 2639
AcyI GRCGYC 2 cut(s) 38, 258
AdeI CACNNNGTG 3 cut(s) 1319, 3564, 3656
AfeI AGCGCT 1 cut(s) 2423
AfiI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2828, 3777
AjnI CCWGG 7 cut(s) 375, 540, 623, 1061, 1436, 3276, 3621
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
Alw21I GWGCWC 1 cut(s) 3312
Alw26I GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
AlwI GGATC 4 cut(s) 1518, 1934, 2712, 3260
Ama87I CYCGRG 3 cut(s) 242, 681, 2285
Aor51HI AGCGCT 1 cut(s) 2423
ApeKI GCWGC 7 cut(s) 319, 1515, 2111, 3394, 3500, 3503, 3774
ApoI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3202, 3547
AspLEI GCGC 5 cut(s) 53, 502, 621, 2424, 3439
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 4 cut(s) 236, 610, 2051, 2336
AsuII TTCGAA 2 cut(s) 252, 1713
AvaI CYCGRG 3 cut(s) 242, 681, 2285
AvaII GGWCC 5 cut(s) 1072, 2413, 2726, 3229, 3625
BaeGI GKGCMC 1 cut(s) 2759
BalI TGGCCA 2 cut(s) 968, 1066
BanII GRGCYC 1 cut(s) 3312
BarI GAAGNNNNNNTAC 6 cut(s) 26, 58, 2169, 2201, 2824, 2856
BauI CACGAG 2 cut(s) 71, 356
BbsI GAAGAC 3 cut(s) 261, 882, 2184
Bbv12I GWGCWC 1 cut(s) 3312
BbvCI CCTCAGC 2 cut(s) 294, 662
BbvI GCAGC 7 cut(s) 331, 1502, 2098, 3406, 3487, 3490, 3761
BccI CCATC 6 cut(s) 68, 174, 608, 2399, 2403, 3737
BceAI ACGGC 3 cut(s) 1518, 1725, 2914
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 7 cut(s) 377, 542, 625, 1063, 1438, 3278, 3623
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BfmI CTRYAG 4 cut(s) 134, 1336, 1399, 3775
BfoI RGCGCY 1 cut(s) 2425
BfuAI ACCTGC 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 89
BglII AGATCT 2 cut(s) 1934, 3692
BisI GCNGC 8 cut(s) 320, 1516, 2001, 2112, 3395, 3501, 3504, 3775
BlpI GCTNAGC 2 cut(s) 2909, 3140
BlsI GCNGC 8 cut(s) 321, 1517, 2002, 2113, 3396, 3502, 3505, 3776
BmcAI AGTACT 4 cut(s) 182, 413, 2664, 2967
Bme1390I CCNGG 8 cut(s) 85, 377, 542, 625, 1063, 1438, 3278, 3623
Bme18I GGWCC 5 cut(s) 1072, 2413, 2726, 3229, 3625
BmeT110I CYCGRG 3 cut(s) 242, 681, 2285
BmiI GGNNCC 2 cut(s) 2414, 3620
BmrFI CCNGG 8 cut(s) 85, 377, 542, 625, 1063, 1438, 3278, 3623
BmsI GCATC 4 cut(s) 276, 844, 2803, 3515
BpiI GAAGAC 3 cut(s) 261, 882, 2184
BplI GAGNNNNNCTC 4 cut(s) 1040, 1072, 1949, 1981
BpmI CTGGAG 2 cut(s) 1989, 2525
Bpu10I CCTNAGC 4 cut(s) 294, 662, 1175, 3629
Bpu1102I GCTNAGC 2 cut(s) 2909, 3140
Bpu14I TTCGAA 2 cut(s) 252, 1713
BpuEI CTTGAG 3 cut(s) 929, 992, 3155
BpuMI CCSGG 1 cut(s) 85
BsaBI GATNNNNATC 3 cut(s) 165, 1776, 3691
BsaHI GRCGYC 2 cut(s) 38, 258
BsaI GGTCTC 1 cut(s) 1427
BsaJI CCNNGG 3 cut(s) 220, 375, 858
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2828, 3777
Bse1I ACTGG 4 cut(s) 1878, 2247, 2989, 3568
Bse3DI GCAATG 3 cut(s) 2610, 2710, 3184
Bse8I GATNNNNATC 3 cut(s) 165, 1776, 3691
BseBI CCWGG 7 cut(s) 377, 542, 625, 1063, 1438, 3278, 3623
BseDI CCNNGG 3 cut(s) 220, 375, 858
BseGI GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3554, 3729
BseJI GATNNNNATC 3 cut(s) 165, 1776, 3691
BseLI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2828, 3777
BseMI GCAATG 3 cut(s) 2610, 2710, 3184
BseNI ACTGG 4 cut(s) 1878, 2247, 2989, 3568
BseRI GAGGAG 4 cut(s) 26, 89, 92, 311
BseSI GKGCMC 1 cut(s) 2759
BseXI GCAGC 7 cut(s) 331, 1502, 2098, 3406, 3487, 3490, 3761
BsgI GTGCAG 7 cut(s) 157, 416, 1365, 2097, 3336, 3495, 3678
Bsh1236I CGCG 2 cut(s) 53, 500
BsiHKAI GWGCWC 1 cut(s) 3312
BsiHKCI CYCGRG 3 cut(s) 242, 681, 2285
BsiSI CCGG 2 cut(s) 46, 85
BslFI GGGAC 3 cut(s) 172, 202, 1263
BslI CCNNNNNNNGG 9 cut(s) 76, 147, 226, 547, 595, 663, 1200, 2828, 3777
BsmAI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BsmBI CGTCTC 2 cut(s) 245, 2810
BsmFI GGGAC 3 cut(s) 172, 202, 1263
BsmI GAATGC 4 cut(s) 406, 1749, 2581, 3129
Bso31I GGTCTC 1 cut(s) 1427
BsoBI CYCGRG 3 cut(s) 242, 681, 2285
Bsp119I TTCGAA 2 cut(s) 252, 1713
Bsp1286I GDGCHC 2 cut(s) 2759, 3312
Bsp1407I TGTACA 1 cut(s) 1369
Bsp1720I GCTNAGC 2 cut(s) 2909, 3140
BspACI CCGC 4 cut(s) 2001, 3072, 3329, 3616
BspFNI CGCG 2 cut(s) 53, 500
BspLI GGNNCC 2 cut(s) 2414, 3620
BspMAI CTGCAG 2 cut(s) 138, 3779
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 4 cut(s) 1518, 1934, 2712, 3260
BspQI GCTCTTC 2 cut(s) 927, 3300
BspT104I TTCGAA 2 cut(s) 252, 1713
BspTNI GGTCTC 1 cut(s) 1427
BsrBI CCGCTC 1 cut(s) 2003
BsrDI GCAATG 3 cut(s) 2610, 2710, 3184
BsrGI TGTACA 1 cut(s) 1369
BsrI ACTGG 4 cut(s) 1878, 2247, 2989, 3568
BssECI CCNNGG 3 cut(s) 220, 375, 858
BssNI GRCGYC 2 cut(s) 38, 258
BssSI CACGAG 2 cut(s) 71, 356
BssT1I CCWWGG 1 cut(s) 858
Bst2BI CACGAG 2 cut(s) 71, 356
Bst2UI CCWGG 7 cut(s) 377, 542, 625, 1063, 1438, 3278, 3623
Bst4CI ACNGT 7 cut(s) 779, 892, 1292, 1785, 2530, 2571, 3669
BstACI GRCGYC 2 cut(s) 38, 258
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstAUI TGTACA 1 cut(s) 1369
BstBI TTCGAA 2 cut(s) 252, 1713
BstENI CCTNNNNNAGG 3 cut(s) 224, 545, 3775
BstF5I GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3554, 3729
BstFNI CGCG 2 cut(s) 53, 500
BstH2I RGCGCY 1 cut(s) 2425
BstHHI GCGC 5 cut(s) 53, 502, 621, 2424, 3439
BstMAI GTCTC 8 cut(s) 245, 954, 1043, 1427, 1909, 2086, 2459, 2810
BstNI CCWGG 7 cut(s) 377, 542, 625, 1063, 1438, 3278, 3623
BstNSI RCATGY 3 cut(s) 1575, 2250, 2820
BstSCI CCNGG 8 cut(s) 83, 375, 540, 623, 1061, 1436, 3276, 3621
BstSFI CTRYAG 4 cut(s) 134, 1336, 1399, 3775
BstSLI GKGCMC 1 cut(s) 2759
BstUI CGCG 2 cut(s) 53, 500
BstV1I GCAGC 7 cut(s) 331, 1502, 2098, 3406, 3487, 3490, 3761
BstV2I GAAGAC 3 cut(s) 261, 882, 2184
BstX2I RGATCY 4 cut(s) 1934, 2704, 3252, 3692
BstXI CCANNNNNNTGG 2 cut(s) 2687, 3601
BstYI RGATCY 4 cut(s) 1934, 2704, 3252, 3692
BtsCI GGATG 9 cut(s) 672, 859, 922, 1668, 1906, 2143, 2414, 3554, 3729
BtsIMutI CAGTG 6 cut(s) 1297, 2083, 2235, 2567, 2808, 3558
BveI ACCTGC 1 cut(s) 141
CfoI GCGC 5 cut(s) 53, 502, 621, 2424, 3439
CseI GACGC 4 cut(s) 27, 266, 506, 2117
DraI TTTAAA 1 cut(s) 1623
DraIII CACNNNGTG 3 cut(s) 1319, 3564, 3656
EaeI YGGCCR 3 cut(s) 966, 1064, 1998
Ecl136II GAGCTC 1 cut(s) 3310
Eco130I CCWWGG 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 3312
Eco31I GGTCTC 1 cut(s) 1427
Eco47I GGWCC 5 cut(s) 1072, 2413, 2726, 3229, 3625
Eco47III AGCGCT 1 cut(s) 2423
Eco53kI GAGCTC 1 cut(s) 3310
Eco57I CTGAAG 1 cut(s) 2639
Eco88I CYCGRG 3 cut(s) 242, 681, 2285
EcoICRI GAGCTC 1 cut(s) 3310
EcoNI CCTNNNNNAGG 3 cut(s) 224, 545, 3775
EcoO109I RGGNCCY 2 cut(s) 139, 1072
EcoRI GAATTC 1 cut(s) 3202
EcoRII CCWGG 7 cut(s) 375, 540, 623, 1061, 1436, 3276, 3621
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 3 cut(s) 1573, 2818, 3184
EcoT38I GRGCYC 1 cut(s) 3312
ErhI CCWWGG 1 cut(s) 858
Esp3I CGTCTC 2 cut(s) 245, 2810
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 3 cut(s) 172, 202, 1263
FauNDI CATATG 2 cut(s) 2373, 3358
Fnu4HI GCNGC 8 cut(s) 320, 1516, 2001, 2112, 3395, 3501, 3504, 3775
FokI GGATG 9 cut(s) 679, 866, 929, 1655, 1913, 2150, 2421, 3541, 3716
FriOI GRGCYC 1 cut(s) 3312
Fsp4HI GCNGC 8 cut(s) 320, 1516, 2001, 2112, 3395, 3501, 3504, 3775
GlaI GCGC 5 cut(s) 52, 501, 620, 2423, 3438
GluI GCNGC 8 cut(s) 320, 1516, 2001, 2112, 3395, 3501, 3504, 3775
GsuI CTGGAG 2 cut(s) 1989, 2525
HaeII RGCGCY 1 cut(s) 2425
HapII CCGG 2 cut(s) 46, 85
HgaI GACGC 4 cut(s) 27, 266, 506, 2117
HhaI GCGC 5 cut(s) 53, 502, 621, 2424, 3439
Hin1I GRCGYC 2 cut(s) 38, 258
Hin6I GCGC 5 cut(s) 51, 500, 619, 2422, 3437
HinP1I GCGC 5 cut(s) 51, 500, 619, 2422, 3437
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 3 cut(s) 431, 1358, 2858
HpaII CCGG 2 cut(s) 46, 85
HphI GGTGA 4 cut(s) 236, 610, 2051, 2336
Hpy166II GTNNAC 3 cut(s) 716, 1260, 1745
Hpy8I GTNNAC 3 cut(s) 716, 1260, 1745
HpyCH4III ACNGT 7 cut(s) 779, 892, 1292, 1785, 2530, 2571, 3669
HpyCH4IV ACGT 3 cut(s) 96, 865, 2699
HpySE526I ACGT 3 cut(s) 96, 865, 2699
Hsp92I GRCGYC 2 cut(s) 38, 258
HspAI GCGC 5 cut(s) 51, 500, 619, 2422, 3437
LguI GCTCTTC 2 cut(s) 927, 3300
LmnI GCTCC 5 cut(s) 48, 1468, 2008, 3002, 3444
Lsp1109I GCAGC 7 cut(s) 331, 1502, 2098, 3406, 3487, 3490, 3761
LweI GCATC 4 cut(s) 276, 844, 2803, 3515
MaeII ACGT 3 cut(s) 96, 865, 2699
MaeIII GTNAC 3 cut(s) 2524, 3013, 3076
MbiI CCGCTC 1 cut(s) 2003
MflI RGATCY 4 cut(s) 1934, 2704, 3252, 3692
MhlI GDGCHC 2 cut(s) 2759, 3312
MlsI TGGCCA 2 cut(s) 968, 1066
MluNI TGGCCA 2 cut(s) 968, 1066
MlyI GAGTC 6 cut(s) 1981, 2186, 2234, 2461, 3029, 3381
MmeI TCCRAC 3 cut(s) 2682, 2753, 3364
Mox20I TGGCCA 2 cut(s) 968, 1066
Mph1103I ATGCAT 3 cut(s) 1573, 2818, 3184
MscI TGGCCA 2 cut(s) 968, 1066
MslI CAYNNNNRTG 5 cut(s) 507, 777, 2103, 2480, 3599
Msp20I TGGCCA 2 cut(s) 968, 1066
MspI CCGG 2 cut(s) 46, 85
MspR9I CCNGG 8 cut(s) 85, 377, 542, 625, 1063, 1438, 3278, 3623
Mva1269I GAATGC 4 cut(s) 406, 1749, 2581, 3129
MvaI CCWGG 7 cut(s) 377, 542, 625, 1063, 1438, 3278, 3623
MvnI CGCG 2 cut(s) 53, 500
NciI CCSGG 1 cut(s) 85
NdeI CATATG 2 cut(s) 2373, 3358
NlaIV GGNNCC 2 cut(s) 2414, 3620
NmuCI GTSAC 1 cut(s) 3076
NsiI ATGCAT 3 cut(s) 1573, 2818, 3184
NspI RCATGY 3 cut(s) 1575, 2250, 2820
NspV TTCGAA 2 cut(s) 252, 1713
PaeI GCATGC 3 cut(s) 1575, 2250, 2820
PaeR7I CTCGAG 2 cut(s) 242, 2285
PciSI GCTCTTC 2 cut(s) 927, 3300
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 249
PctI GAATGC 4 cut(s) 406, 1749, 2581, 3129
PfeI GAWTC 6 cut(s) 1139, 1160, 1523, 2339, 3416, 3610
PfoI TCCNGGA 1 cut(s) 3621
PkrI GCNGC 8 cut(s) 321, 1517, 2002, 2113, 3396, 3502, 3505, 3776
PleI GAGTC 6 cut(s) 1981, 2186, 2233, 2461, 3028, 3381
PpsI GAGTC 6 cut(s) 1981, 2186, 2233, 2461, 3028, 3381
PpuMI RGGWCCY 1 cut(s) 1072
Psp124BI GAGCTC 1 cut(s) 3312
Psp1406I AACGTT 1 cut(s) 2699
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 7 cut(s) 375, 540, 623, 1061, 1436, 3276, 3621
PspGI CCWGG 7 cut(s) 375, 540, 623, 1061, 1436, 3276, 3621
PspN4I GGNNCC 2 cut(s) 2414, 3620
PspPPI RGGWCCY 1 cut(s) 1072
PspXI VCTCGAGB 1 cut(s) 2285
PstI CTGCAG 2 cut(s) 138, 3779
PsuI RGATCY 4 cut(s) 1934, 2704, 3252, 3692
RseI CAYNNNNRTG 5 cut(s) 507, 777, 2103, 2480, 3599
SacI GAGCTC 1 cut(s) 3312
SapI GCTCTTC 2 cut(s) 927, 3300
SatI GCNGC 8 cut(s) 320, 1516, 2001, 2112, 3395, 3501, 3504, 3775
SbfI CCTGCAGG 1 cut(s) 138
ScaI AGTACT 4 cut(s) 182, 413, 2664, 2967
SchI GAGTC 6 cut(s) 1981, 2186, 2234, 2461, 3029, 3381
ScrFI CCNGG 8 cut(s) 85, 377, 542, 625, 1063, 1438, 3278, 3623
SdaI CCTGCAGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 2759, 3312
SfaNI GCATC 4 cut(s) 276, 844, 2803, 3515
SfcI CTRYAG 4 cut(s) 134, 1336, 1399, 3775
SfiI GGCCNNNNNGGCC 1 cut(s) 89
Sfr274I CTCGAG 2 cut(s) 242, 2285
SfuI TTCGAA 2 cut(s) 252, 1713
SinI GGWCC 5 cut(s) 1072, 2413, 2726, 3229, 3625
SlaI CTCGAG 2 cut(s) 242, 2285
SmiMI CAYNNNNRTG 5 cut(s) 507, 777, 2103, 2480, 3599
SmlI CTYRAG 5 cut(s) 242, 944, 1007, 2285, 3134
SmoI CTYRAG 5 cut(s) 242, 944, 1007, 2285, 3134
SphI GCATGC 3 cut(s) 1575, 2250, 2820
Sse8387I CCTGCAGG 1 cut(s) 138
SsiI CCGC 4 cut(s) 2001, 3072, 3329, 3616
SstI GAGCTC 1 cut(s) 3312
StyD4I CCNGG 8 cut(s) 83, 375, 540, 623, 1061, 1436, 3276, 3621
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 7 cut(s) 779, 892, 1292, 1785, 2530, 2571, 3669
TaiI ACGT 3 cut(s) 99, 868, 2702
TaqI TCGA 6 cut(s) 153, 243, 252, 1713, 2286, 2773
TatI WGTACW 6 cut(s) 180, 411, 1369, 1529, 2662, 2965
TauI GCSGC 1 cut(s) 2003
TfiI GAWTC 6 cut(s) 1139, 1160, 1523, 2339, 3416, 3610
TscAI CASTG 6 cut(s) 1297, 2083, 2235, 2574, 2815, 3565
TseFI GTSAC 1 cut(s) 3076
TseI GCWGC 7 cut(s) 319, 1515, 2111, 3394, 3500, 3503, 3774
Tsp45I GTSAC 1 cut(s) 3076
TspGWI ACGGA 3 cut(s) 227, 1970, 3015
TspRI CASTG 6 cut(s) 1297, 2083, 2235, 2574, 2815, 3565
VpaK11BI GGWCC 5 cut(s) 1072, 2413, 2726, 3229, 3625
XagI CCTNNNNNAGG 3 cut(s) 224, 545, 3775
XapI RAATTY 7 cut(s) 835, 1079, 1113, 1221, 1892, 3202, 3547
XceI RCATGY 3 cut(s) 1575, 2250, 2820
XcmI CCANNNNNNNNNTGG 1 cut(s) 2687
XhoI CTCGAG 2 cut(s) 242, 2285
ZrmI AGTACT 4 cut(s) 182, 413, 2664, 2967
Zsp2I ATGCAT 3 cut(s) 1573, 2818, 3184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.