MD00G1176800.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007977
Physical Location & Seq
Reverse (-)
41115158 .. 41116156
999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1176800.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGACGTCCGAGAGGAAAATGGAAGGCGACATGCCTTGCCTTGTGGCTTTCTTCTTGGACGAACTATTGCTTGTCTTACGACCTTGACATTGGGAACATCAATGGTGGCTCGTCAAGGTCTAATAGCTATGGCTGCTCATCAGATATGTATTCAAGTATGGTTGGCTGTATCCCTTTTAACTGGTGCAATGGCTGCATCTGGTCAGGCCCTGATTGCAAGTTACTTATCAAAAGGTGAATACAAAATTGTGAAAGAAGTTGTTGACTCTGTGTTAAAGATAGGATTGTTCACAGGTATTTCCTTGTCTGTAATTTTGGGAGTACCTTTTGGTTCTCTAGCTACGTTGTTCACCAACGATCCTGAAGTATTAGCTATTGTTAGATCTGGGATATTGTTTGTCAGTGCTAGTCAGCGTTTGAATGCTCTAGCTTACGTTTTTTATGGCCTCCATTATGGTGTTTCAGATTTTGCATATGCTGCTCGCTCCATGGTAAGCTTGCTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

170

Amino Acids

18.0

Weight (kDa)

8.88

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 31 - 165 2.4e-09 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
AclWI GGATC 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 1 cut(s) 324
AgsI TTSAA 2 cut(s) 155, 421
AluBI AGCT 5 cut(s) 128, 341, 374, 431, 498
AluI AGCT 5 cut(s) 128, 341, 374, 431, 498
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 211
AoxI GGCC 2 cut(s) 207, 445
ApeKI GCWGC 3 cut(s) 134, 194, 479
AspS9I GGNCC 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 248, 343
BbvI GCAGC 3 cut(s) 121, 181, 466
BciVI GTATCC 1 cut(s) 181
BfaI CTAG 3 cut(s) 338, 408, 428
BfuI GTATCC 1 cut(s) 181
BglII AGATCT 1 cut(s) 383
BisI GCNGC 3 cut(s) 135, 195, 480
BlsI GCNGC 3 cut(s) 136, 196, 481
BmgT120I GGNCC 1 cut(s) 208
BmsI GCATC 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 3 cut(s) 121, 181, 466
BshFI GGCC 2 cut(s) 209, 447
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 2 cut(s) 209, 447
Bsp143I GATC 2 cut(s) 358, 383
Bsp19I CCATGG 1 cut(s) 489
BspANI GGCC 2 cut(s) 209, 447
BspPI GGATC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 2 cut(s) 358, 383
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 489
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 2 cut(s) 194, 479
BstC8I GCNNGC 2 cut(s) 484, 500
BstDSI CCRYGG 1 cut(s) 489
BstKTI GATC 2 cut(s) 361, 386
BstMBI GATC 2 cut(s) 358, 383
BstMWI GCNNNNNNNGC 4 cut(s) 134, 194, 215, 479
BstNSI RCATGY 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 121, 181, 466
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 209, 447
BtgI CCRYGG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 484, 500
CaiI CAGNNNCTG 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 323
CviAII CATG 2 cut(s) 32, 490
CviQI GTAC 1 cut(s) 323
DpnI GATC 2 cut(s) 360, 385
DpnII GATC 2 cut(s) 358, 383
Eco130I CCWWGG 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 2 cut(s) 35, 493
FaiI YATR 9 cut(s) 33, 131, 148, 160, 444, 456, 475, 477, 491
FatI CATG 2 cut(s) 31, 489
FauNDI CATATG 1 cut(s) 475
Fnu4HI GCNGC 3 cut(s) 135, 195, 480
Fsp4HI GCNGC 3 cut(s) 135, 195, 480
FspBI CTAG 3 cut(s) 338, 408, 428
GluI GCNGC 3 cut(s) 135, 195, 480
HaeIII GGCC 2 cut(s) 209, 447
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 35, 493
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 1 cut(s) 266
HphI GGTGA 2 cut(s) 248, 343
Hpy166II GTNNAC 3 cut(s) 265, 291, 351
Hpy188I TCNGA 3 cut(s) 11, 144, 466
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 3 cut(s) 265, 291, 351
HpyAV CCTTC 1 cut(s) 18
HpyCH4IV ACGT 3 cut(s) 6, 344, 435
HpyCH4V TGCA 4 cut(s) 188, 197, 218, 473
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 194, 215, 479
HpySE526I ACGT 3 cut(s) 6, 344, 435
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 35, 493
Kzo9I GATC 2 cut(s) 358, 383
LmnI GCTCC 1 cut(s) 491
LpnPI CCDG 7 cut(s) 168, 186, 191, 224, 279, 372, 375
Lsp1109I GCAGC 3 cut(s) 121, 181, 466
LweI GCATC 1 cut(s) 206
MaeI CTAG 3 cut(s) 338, 408, 428
MaeII ACGT 3 cut(s) 6, 344, 435
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 360, 385
MboI GATC 2 cut(s) 358, 383
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 383
MluCI AATT 2 cut(s) 246, 312
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 2 cut(s) 7, 458
MseI TTAA 2 cut(s) 179, 275
MslI CAYNNNNRTG 1 cut(s) 456
Mva1269I GAATGC 1 cut(s) 427
MwoI GCNNNNNNNGC 4 cut(s) 134, 194, 215, 479
NcoI CCATGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 475
NdeII GATC 2 cut(s) 358, 383
NlaIII CATG 2 cut(s) 35, 493
NspI RCATGY 1 cut(s) 35
PctI GAATGC 1 cut(s) 427
PkrI GCNGC 3 cut(s) 136, 196, 481
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 383
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 2 cut(s) 179, 275
SatI GCNGC 3 cut(s) 135, 195, 480
Sau3AI GATC 2 cut(s) 358, 383
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 260
SfaNI GCATC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 456
Sse9I AATT 2 cut(s) 246, 312
SspMI CTAG 3 cut(s) 338, 408, 428
StyI CCWWGG 1 cut(s) 489
TaiI ACGT 3 cut(s) 9, 347, 438
TasI AATT 2 cut(s) 246, 312
Tru1I TTAA 2 cut(s) 179, 275
Tru9I TTAA 2 cut(s) 179, 275
TscAI CASTG 1 cut(s) 409
TseI GCWGC 3 cut(s) 134, 194, 479
TspRI CASTG 1 cut(s) 409
XceI RCATGY 1 cut(s) 35
XspI CTAG 3 cut(s) 338, 408, 428
ZraI GACGTC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.