Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0007977

Physical Location & Seq

Genomic Coordinates Forward (+)

25589042 .. 25592748

Gene Structure 3707 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1165300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGCGATTGGTCTCTCACACCACCACCTTCTCTGCATATATTCCTCCAAATCCAACCCACCATTTTCAAAAACGCAAACACATGTCAGAAACCCGAAATCCTCCTCCGTCGCTCTTCGGACCATCCTTAAGTCGTCGTCTCCGAAAAACTCCAACACTTCGCTGCAAAGTTCGCCGGAAGAATCGAAGCCCGCCATCACTTCTAAGTGGGCTGACCCGCCCGGCAAAAACCCACCTCCCAGATTGTCTTCTTTCGACTCCCTCTTCATCCGCCTTCGTCGTTTAAGAGCTGAAAGGTTTAAACTTGATGGAATTTTTGTGGACATTTTGTCGATTGCTCTGCCTGCTGCTCTGGCGCTGGCTGCTGACCCTATTACCTCTTTGGTTGACACAGCCTTTGTTGGCCATTTAGGGTCGGTTGAATTGGCAGCAGTTGGGGTGTCAGCTTCAGTATTCAATTTAGTTTCTAAATTGTTTAACGTTCCGTTGCTCAACATCACCACATCTTTTGTTGCTGAAGATCAGGCATTGGCTAGCAAGGCCCAGGATGATTTCCAGGGTGACAGCCAGAGCAAGAAGCTCCTTCCCTCAGTGTCAACTTCGTTGGCACTTGCTGCCACCATTGGCATTGCTGAAGCTGTTGCACTTTTTCTTGGGTCGGGTGTCTTGATGAATGCCATGGGTATATATGCTGATTCACCAATGCGAATACCTGCTGAGAATTTTCTTGCCCTAAGGGCATTAGGTGCTCCACCAATTGTAATTGCACTTGCCTCACAAGGCACTTTTCGTGGCTTTAAGGACACAAAGACACCTCTGTATGCCATTGTTGCTGGCAACTTACTTAATGCTATATTGGATGCAATTTTGATATTTGCTTTTGATTTTGGAATCCGCGGTGCTGCTATTGCTACAGTGATTTCTGAGTACGTCAACGATGTGAACTCTTCATGTCTATTTTTCGCAAGGTACCTGATTGCTGCTATTCTTCTGTGGAAATTGAATAGCAAAGTATTACTTGTCCCTACCTATATTGATGGGGGAAGAATAATCGGTTACCTTAAATCTGGGGGTCTTCTTATTGGCAGAAGCTTAGCAGTAGTTGTAACTACGACACTAGCCACATCCATGGCAGCCCGGGAAGGTCCTATCCCCATGGCTGGCCATCAGATTTGCATTCAAGTTTGGTTGGCAGTATCTTTGCTTACCGATGCTTTAGCACTTGCTGGTCAGGCACTTCTTGCCAGTGGTTACTCCCAACAAGATTATGAACAAGCACGCCTAGTGATTTATAGGGTTCTACAGATTGGTTTAGTGACAGGAATTGGTTTGGGAATTATATTATTCATCAGTTTTAAAGCGTTTTCTAGCTTATTCAGCACAGACCCACAAGTCCTGGCAATTGCATGGTCTGGTTTATTGTTTGTTGCTGGATCTCAGCCAATAAACGCTATAGCATTTGTTATCGACGGACTGTACTATGGAGTTTCAGACTTTGGATATGCTGCCTATTCAATGGTGTTAGTAGGGCTGATTTCGTCCACAGTCATACTCATTGCTTCTCCGTCTTTTGGCCTTGCTGGAGTCTGGACGGGGCTGTTTCTCTTCATGGCATTGCGCGTAGTAGCCGGGATTTGGAGGTTGGGCACAAGAACCGGACCATGGAAATTGGTCTGGGATGAGAAGAAGCAGGAAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MIF MatE

Protein Analysis

567

Amino Acids

60.2

Weight (kDa)

9.3

Isoelectric Point (pI)

32.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	115 - 288	3.8e-15	MatE
MatE	PF01554	363 - 508	2.8e-11	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38330
fragaria_vesca	FvH4_6g10700 FvH4_6g10700
malus_domestica	MD00G1176800.v1.1 MD04G1165300.v1.1 MD12G1179400.v1.1
prunus_persica	Prupe.6G284300_v2.0.a1
pyrus_communis	pycom04g14730 pycom04g14740 pycom12g16920
rosa_chinensis	RchiOBHm_Chr3g0461701
rosa_laevigata	RLG00000024904
rosa_multiflora	Rmu_co8339943.1_g000001 Rmu_co8373083.1_g000001 Rmu_sc0012406.1_g000005 Rmu_sc0027127.1_g000001 Rmu_sc0038320.1_g000003
rosa_roxburghii	Rroxscaffold_6G00418030
rosa_rugosa	Rorug03G0053100
rosa_samantha	Rh3AG111300 Rh3BG114300 Rh3CG116500 Rh3DG116200
rosa_wichuraiana	Rw3G009420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1391
Acc36I	ACCTGC	1 cut(s)	721
Acc65I	GGTACC	1 cut(s)	969
AccB1I	GGYRCC	1 cut(s)	969
AccII	CGCG	2 cut(s)	897, 1620
AciI	CCGC	5 cut(s)	192, 218, 271, 895, 897
AclI	AACGTT	1 cut(s)	480
AclWI	GGATC	1 cut(s)	1441
AcoI	YGGCCR	2 cut(s)	403, 1162
AcsI	RAATTY	2 cut(s)	312, 721
AcuI	CTGAAG	3 cut(s)	432, 537, 654
AfaI	GTAC	3 cut(s)	929, 971, 1478
AfiI	CCNNNNNNNGG	4 cut(s)	1160, 1571, 1635, 1662
AflII	CTTAAG	1 cut(s)	128
AflIII	ACRYGT	1 cut(s)	82
AgsI	TTSAA	6 cut(s)	69, 422, 457, 1003, 1181, 1515
AhdI	GACNNNNNGTC	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	543, 555, 1395
AluBI	AGCT	6 cut(s)	290, 446, 580, 638, 1092, 1371
AluI	AGCT	6 cut(s)	290, 446, 580, 638, 1092, 1371
Alw21I	GWGCWC	1 cut(s)	751
Alw26I	GTCTC	2 cut(s)	17, 144
AlwI	GGATC	1 cut(s)	1441
Ama87I	CYCGRG	1 cut(s)	1137
AoxI	GGCC	4 cut(s)	403, 540, 1162, 1573
ApeKI	GCWGC	9 cut(s)	163, 347, 362, 428, 614, 902, 980, 1133, 1505
ApoI	RAATTY	2 cut(s)	312, 721
ArsI	GACNNNNNNTTYG	2 cut(s)	380, 412
Asp718I	GGTACC	1 cut(s)	969
AspLEI	GCGC	2 cut(s)	358, 1620
AspS9I	GGNCC	4 cut(s)	120, 541, 1145, 1658
AsuC2I	CCSGG	4 cut(s)	222, 1138, 1139, 1630
AsuHPI	GGTGA	3 cut(s)	490, 572, 690
AsuNHI	GCTAGC	1 cut(s)	533
AvaI	CYCGRG	1 cut(s)	1137
AvaII	GGWCC	3 cut(s)	120, 1145, 1658
AxyI	CCTNAGG	1 cut(s)	734
BaeGI	GKGCMC	1 cut(s)	1649
BalI	TGGCCA	2 cut(s)	405, 1164
BanI	GGYRCC	1 cut(s)	969
BbsI	GAAGAC	2 cut(s)	240, 1067
Bbv12I	GWGCWC	1 cut(s)	751
BbvI	GCAGC	9 cut(s)	150, 334, 349, 440, 601, 889, 967, 1145, 1492
BccI	CCATC	5 cut(s)	131, 203, 302, 1031, 1173
BcgI	CGANNNNNNTGC	2 cut(s)	322, 356
BciT130I	CCWGG	3 cut(s)	545, 557, 1397
BcnI	CCSGG	4 cut(s)	222, 1138, 1139, 1630
BcoDI	GTCTC	2 cut(s)	17, 144
BfaI	CTAG	4 cut(s)	534, 1118, 1283, 1368
BfmI	CTRYAG	3 cut(s)	912, 1301, 1452
BfoI	RGCGCY	1 cut(s)	359
BfrI	CTTAAG	1 cut(s)	128
BfuAI	ACCTGC	1 cut(s)	721
BglI	GCCNNNNNGGC	1 cut(s)	737
BisI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 903, 981, 1134, 1506
BlpI	GCTNAGC	1 cut(s)	1093
BlsI	GCNGC	9 cut(s)	165, 349, 364, 430, 616, 904, 982, 1135, 1507
Bme1390I	CCNGG	7 cut(s)	222, 545, 557, 1138, 1139, 1397, 1630
Bme18I	GGWCC	3 cut(s)	120, 1145, 1658
BmeRI	GACNNNNNGTC	1 cut(s)	328
BmeT110I	CYCGRG	1 cut(s)	1137
BmgT120I	GGNCC	4 cut(s)	120, 541, 1145, 1658
BmiI	GGNNCC	1 cut(s)	971
BmrFI	CCNGG	7 cut(s)	222, 545, 557, 1138, 1139, 1397, 1630
BmsI	GCATC	2 cut(s)	850, 1201
BmtI	GCTAGC	1 cut(s)	537
BpiI	GAAGAC	2 cut(s)	240, 1067
BpmI	CTGGAG	1 cut(s)	1602
Bpu1102I	GCTNAGC	1 cut(s)	1093
BpuMI	CCSGG	4 cut(s)	222, 1138, 1139, 1630
BsaI	GGTCTC	1 cut(s)	17
BsaJI	CCNNGG	8 cut(s)	543, 556, 678, 895, 1128, 1137, 1155, 1661
BsaWI	WCCGGW	1 cut(s)	1655
Bsc4I	CCNNNNNNNGG	4 cut(s)	1160, 1571, 1635, 1662
Bse1I	ACTGG	1 cut(s)	1245
Bse21I	CCTNAGG	1 cut(s)	734
Bse3DI	GCAATG	3 cut(s)	627, 1554, 1613
BseBI	CCWGG	3 cut(s)	545, 557, 1397
BseDI	CCNNGG	8 cut(s)	543, 556, 678, 895, 1128, 1137, 1155, 1661
BseGI	GGATG	6 cut(s)	123, 267, 553, 865, 1124, 1684
BseLI	CCNNNNNNNGG	4 cut(s)	1160, 1571, 1635, 1662
BseMI	GCAATG	3 cut(s)	627, 1554, 1613
BseMII	CTCAG	4 cut(s)	603, 708, 915, 1451
BseNI	ACTGG	1 cut(s)	1245
BseRI	GAGGAG	1 cut(s)	94
BseSI	GKGCMC	1 cut(s)	1649
BseXI	GCAGC	9 cut(s)	150, 334, 349, 440, 601, 889, 967, 1145, 1492
Bsh1236I	CGCG	2 cut(s)	897, 1620
BshFI	GGCC	4 cut(s)	405, 542, 1164, 1575
BshNI	GGYRCC	1 cut(s)	969
BsiHKAI	GWGCWC	1 cut(s)	751
BsiHKCI	CYCGRG	1 cut(s)	1137
BsiSI	CCGG	5 cut(s)	176, 222, 1138, 1629, 1656
BslFI	GGGAC	1 cut(s)	1007
BslI	CCNNNNNNNGG	4 cut(s)	1160, 1571, 1635, 1662
BsmAI	GTCTC	2 cut(s)	17, 144
BsmBI	CGTCTC	1 cut(s)	144
BsmFI	GGGAC	1 cut(s)	1007
BsmI	GAATGC	2 cut(s)	679, 1176
BsnI	GGCC	4 cut(s)	405, 542, 1164, 1575
Bso31I	GGTCTC	1 cut(s)	17
BsoBI	CYCGRG	1 cut(s)	1137
Bsp1286I	GDGCHC	2 cut(s)	751, 1649
Bsp143I	GATC	2 cut(s)	520, 1433
Bsp1720I	GCTNAGC	1 cut(s)	1093
Bsp19I	CCATGG	4 cut(s)	678, 1128, 1155, 1661
BspACI	CCGC	5 cut(s)	192, 218, 271, 895, 897
BspANI	GGCC	4 cut(s)	405, 542, 1164, 1575
BspCNI	CTCAG	4 cut(s)	602, 709, 916, 1450
BspFNI	CGCG	2 cut(s)	897, 1620
BspLI	GGNNCC	1 cut(s)	971
BspMI	ACCTGC	1 cut(s)	721
BspOI	GCTAGC	1 cut(s)	537
BspPI	GGATC	1 cut(s)	1441
BspQI	GCTCTTC	1 cut(s)	120
BspT107I	GGYRCC	1 cut(s)	969
BspTI	CTTAAG	1 cut(s)	128
BspTNI	GGTCTC	1 cut(s)	17
BsrDI	GCAATG	3 cut(s)	627, 1554, 1613
BsrI	ACTGG	1 cut(s)	1245
BssECI	CCNNGG	8 cut(s)	543, 556, 678, 895, 1128, 1137, 1155, 1661
BssMI	GATC	2 cut(s)	520, 1433
BssT1I	CCWWGG	4 cut(s)	678, 1128, 1155, 1661
Bst2UI	CCWGG	3 cut(s)	545, 557, 1397
Bst4CI	ACNGT	3 cut(s)	916, 1476, 1546
Bst6I	CTCTTC	4 cut(s)	120, 269, 952, 1610
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	3 cut(s)	614, 746, 1241
BstC8I	GCNNGC	7 cut(s)	192, 345, 360, 535, 835, 1162, 1279
BstDEI	CTNAG	8 cut(s)	204, 589, 717, 734, 924, 1093, 1437, 1698
BstDSI	CCRYGG	5 cut(s)	678, 895, 1128, 1155, 1661
BstEII	GGTNACC	1 cut(s)	1055
BstF5I	GGATG	6 cut(s)	123, 267, 553, 865, 1124, 1684
BstFNI	CGCG	2 cut(s)	897, 1620
BstH2I	RGCGCY	1 cut(s)	359
BstHHI	GCGC	2 cut(s)	358, 1620
BstKTI	GATC	2 cut(s)	523, 1436
BstMAI	GTCTC	2 cut(s)	17, 144
BstMBI	GATC	2 cut(s)	520, 1433
BstNI	CCWGG	3 cut(s)	545, 557, 1397
BstNSI	RCATGY	1 cut(s)	86
BstPI	GGTNACC	1 cut(s)	1055
BstSCI	CCNGG	7 cut(s)	220, 543, 555, 1136, 1137, 1395, 1628
BstSFI	CTRYAG	3 cut(s)	912, 1301, 1452
BstSLI	GKGCMC	1 cut(s)	1649
BstUI	CGCG	2 cut(s)	897, 1620
BstV1I	GCAGC	9 cut(s)	150, 334, 349, 440, 601, 889, 967, 1145, 1492
BstV2I	GAAGAC	2 cut(s)	240, 1067
BstX2I	RGATCY	1 cut(s)	1433
BstXI	CCANNNNNNTGG	1 cut(s)	1129
BstYI	RGATCY	1 cut(s)	1433
Bsu36I	CCTNAGG	1 cut(s)	734
BsuRI	GGCC	4 cut(s)	405, 542, 1164, 1575
BtgI	CCRYGG	5 cut(s)	678, 895, 1128, 1155, 1661
BtsCI	GGATG	6 cut(s)	123, 267, 553, 865, 1124, 1684
BtsIMutI	CAGTG	3 cut(s)	597, 921, 1252
BveI	ACCTGC	1 cut(s)	721
Cac8I	GCNNGC	7 cut(s)	192, 345, 360, 535, 835, 1162, 1279
CfoI	GCGC	2 cut(s)	358, 1620
Cfr13I	GGNCC	4 cut(s)	120, 541, 1145, 1658
Cfr42I	CCGCGG	1 cut(s)	898
Cfr9I	CCCGGG	1 cut(s)	1137
Csp6I	GTAC	3 cut(s)	928, 970, 1477
CspCI	CAANNNNNGTGG	2 cut(s)	490, 525
CviAII	CATG	8 cut(s)	83, 679, 951, 1129, 1156, 1407, 1609, 1662
CviQI	GTAC	3 cut(s)	928, 970, 1477
DdeI	CTNAG	8 cut(s)	204, 589, 717, 734, 924, 1093, 1437, 1698
DpnI	GATC	2 cut(s)	522, 1435
DpnII	GATC	2 cut(s)	520, 1433
DraI	TTTAAA	2 cut(s)	301, 1357
DrdI	GACNNNNNNGTC	1 cut(s)	1391
DriI	GACNNNNNGTC	1 cut(s)	328
DseDI	GACNNNNNNGTC	1 cut(s)	1391
EaeI	YGGCCR	2 cut(s)	403, 1162
Eam1104I	CTCTTC	4 cut(s)	120, 269, 952, 1610
Eam1105I	GACNNNNNGTC	1 cut(s)	328
EarI	CTCTTC	4 cut(s)	120, 269, 952, 1610
EciI	GGCGGA	1 cut(s)	260
Eco130I	CCWWGG	4 cut(s)	678, 1128, 1155, 1661
Eco31I	GGTCTC	1 cut(s)	17
Eco47I	GGWCC	3 cut(s)	120, 1145, 1658
Eco57I	CTGAAG	3 cut(s)	432, 537, 654
Eco81I	CCTNAGG	1 cut(s)	734
Eco88I	CYCGRG	1 cut(s)	1137
Eco91I	GGTNACC	1 cut(s)	1055
EcoO109I	RGGNCCY	1 cut(s)	1145
EcoO65I	GGTNACC	1 cut(s)	1055
EcoRII	CCWGG	3 cut(s)	543, 555, 1395
EcoT14I	CCWWGG	4 cut(s)	678, 1128, 1155, 1661
ErhI	CCWWGG	4 cut(s)	678, 1128, 1155, 1661
Esp3I	CGTCTC	1 cut(s)	144
FaeI	CATG	8 cut(s)	86, 682, 954, 1132, 1159, 1410, 1612, 1665
FalI	AAGNNNNNCTT	2 cut(s)	1002, 1034
FaqI	GGGAC	1 cut(s)	1007
FatI	CATG	8 cut(s)	82, 678, 950, 1128, 1155, 1406, 1608, 1661
FauI	CCCGC	2 cut(s)	199, 225
Fnu4HI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 903, 981, 1134, 1506
FokI	GGATG	6 cut(s)	110, 254, 560, 872, 1111, 1691
Fsp4HI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 903, 981, 1134, 1506
FspBI	CTAG	4 cut(s)	534, 1118, 1283, 1368
GlaI	GCGC	2 cut(s)	357, 1619
GluI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 903, 981, 1134, 1506
GsuI	CTGGAG	1 cut(s)	1602
HaeII	RGCGCY	1 cut(s)	359
HaeIII	GGCC	4 cut(s)	405, 542, 1164, 1575
HapII	CCGG	5 cut(s)	176, 222, 1138, 1629, 1656
HhaI	GCGC	2 cut(s)	358, 1620
Hin1II	CATG	8 cut(s)	86, 682, 954, 1132, 1159, 1410, 1612, 1665
Hin6I	GCGC	2 cut(s)	356, 1618
HinP1I	GCGC	2 cut(s)	356, 1618
HincII	GTYRAC	3 cut(s)	388, 597, 934
HindII	GTYRAC	3 cut(s)	388, 597, 934
HindIII	AAGCTT	1 cut(s)	1090
HinfI	GANTC	5 cut(s)	182, 257, 695, 891, 1584
HpaII	CCGG	5 cut(s)	176, 222, 1138, 1629, 1656
HphI	GGTGA	3 cut(s)	490, 572, 690
Hpy166II	GTNNAC	6 cut(s)	322, 388, 597, 934, 943, 1542
Hpy188I	TCNGA	6 cut(s)	89, 120, 144, 925, 1170, 1492
Hpy188III	TCNNGA	2 cut(s)	667, 1588
Hpy8I	GTNNAC	6 cut(s)	322, 388, 597, 934, 943, 1542
Hpy99I	CGWCG	4 cut(s)	113, 139, 282, 1472
HpyAV	CCTTC	4 cut(s)	38, 284, 593, 1136
HpyCH4III	ACNGT	3 cut(s)	916, 1476, 1546
HpyCH4IV	ACGT	2 cut(s)	480, 930
HpyCH4V	TGCA	7 cut(s)	36, 166, 644, 767, 863, 1176, 1406
HpyF3I	CTNAG	8 cut(s)	204, 589, 717, 734, 924, 1093, 1437, 1698
HpySE526I	ACGT	2 cut(s)	480, 930
Hsp92II	CATG	8 cut(s)	86, 682, 954, 1132, 1159, 1410, 1612, 1665
HspAI	GCGC	2 cut(s)	356, 1618
KpnI	GGTACC	1 cut(s)	973
KspI	CCGCGG	1 cut(s)	898
Kzo9I	GATC	2 cut(s)	520, 1433
LguI	GCTCTTC	1 cut(s)	120
LmnI	GCTCC	2 cut(s)	585, 754
Lsp1109I	GCAGC	9 cut(s)	150, 334, 349, 440, 601, 889, 967, 1145, 1492
LweI	GCATC	2 cut(s)	850, 1201
MaeI	CTAG	4 cut(s)	534, 1118, 1283, 1368
MaeII	ACGT	2 cut(s)	480, 930
MaeIII	GTNAC	5 cut(s)	560, 1055, 1105, 1250, 1315
MalI	GATC	2 cut(s)	522, 1435
MboI	GATC	2 cut(s)	520, 1433
MfeI	CAATTG	2 cut(s)	756, 1401
MflI	RGATCY	1 cut(s)	1433
MhlI	GDGCHC	2 cut(s)	751, 1649
MlsI	TGGCCA	2 cut(s)	405, 1164
MluNI	TGGCCA	2 cut(s)	405, 1164
MlyI	GAGTC	2 cut(s)	251, 1593
MmeI	TCCRAC	2 cut(s)	78, 177
Mox20I	TGGCCA	2 cut(s)	405, 1164
MscI	TGGCCA	2 cut(s)	405, 1164
MseI	TTAA	8 cut(s)	129, 284, 300, 477, 798, 846, 1062, 1356
MslI	CAYNNNNRTG	1 cut(s)	1127
Msp20I	TGGCCA	2 cut(s)	405, 1164
MspA1I	CMGCKG	1 cut(s)	897
MspCI	CTTAAG	1 cut(s)	128
MspI	CCGG	5 cut(s)	176, 222, 1138, 1629, 1656
MspR9I	CCNGG	7 cut(s)	222, 545, 557, 1138, 1139, 1397, 1630
MssI	GTTTAAAC	1 cut(s)	301
MunI	CAATTG	2 cut(s)	756, 1401
Mva1269I	GAATGC	2 cut(s)	679, 1176
MvaI	CCWGG	3 cut(s)	545, 557, 1397
MvnI	CGCG	2 cut(s)	897, 1620
NciI	CCSGG	4 cut(s)	222, 1138, 1139, 1630
NcoI	CCATGG	4 cut(s)	678, 1128, 1155, 1661
NdeII	GATC	2 cut(s)	520, 1433
NheI	GCTAGC	1 cut(s)	533
NlaIII	CATG	8 cut(s)	86, 682, 954, 1132, 1159, 1410, 1612, 1665
NlaIV	GGNNCC	1 cut(s)	971
NmuCI	GTSAC	2 cut(s)	560, 1315
NspI	RCATGY	1 cut(s)	86
PciI	ACATGT	1 cut(s)	82
PciSI	GCTCTTC	1 cut(s)	120
PcsI	WCGNNNNNNNCGW	1 cut(s)	140
PctI	GAATGC	2 cut(s)	679, 1176
PfeI	GAWTC	3 cut(s)	182, 695, 891
PkrI	GCNGC	9 cut(s)	165, 349, 364, 430, 616, 904, 982, 1135, 1507
PleI	GAGTC	2 cut(s)	251, 1592
PmeI	GTTTAAAC	1 cut(s)	301
PpsI	GAGTC	2 cut(s)	251, 1592
PpuMI	RGGWCCY	1 cut(s)	1145
PscI	ACATGT	1 cut(s)	82
Psp1406I	AACGTT	1 cut(s)	480
Psp5II	RGGWCCY	1 cut(s)	1145
Psp6I	CCWGG	3 cut(s)	543, 555, 1395
PspEI	GGTNACC	1 cut(s)	1055
PspGI	CCWGG	3 cut(s)	543, 555, 1395
PspN4I	GGNNCC	1 cut(s)	971
PspPI	GGNCC	4 cut(s)	120, 541, 1145, 1658
PspPPI	RGGWCCY	1 cut(s)	1145
PsuI	RGATCY	1 cut(s)	1433
RsaI	GTAC	3 cut(s)	929, 971, 1478
RsaNI	GTAC	3 cut(s)	928, 970, 1477
RseI	CAYNNNNRTG	1 cut(s)	1127
SacII	CCGCGG	1 cut(s)	898
SapI	GCTCTTC	1 cut(s)	120
SaqAI	TTAA	8 cut(s)	129, 284, 300, 477, 798, 846, 1062, 1356
SatI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 903, 981, 1134, 1506
Sau3AI	GATC	2 cut(s)	520, 1433
Sau96I	GGNCC	4 cut(s)	120, 541, 1145, 1658
SchI	GAGTC	2 cut(s)	251, 1593
ScrFI	CCNGG	7 cut(s)	222, 545, 557, 1138, 1139, 1397, 1630
SduI	GDGCHC	2 cut(s)	751, 1649
SfaNI	GCATC	2 cut(s)	850, 1201
SfcI	CTRYAG	3 cut(s)	912, 1301, 1452
Sfr303I	CCGCGG	1 cut(s)	898
SgrBI	CCGCGG	1 cut(s)	898
SinI	GGWCC	3 cut(s)	120, 1145, 1658
SmaI	CCCGGG	1 cut(s)	1139
SmiMI	CAYNNNNRTG	1 cut(s)	1127
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
SsiI	CCGC	5 cut(s)	192, 218, 271, 895, 897
SspMI	CTAG	4 cut(s)	534, 1118, 1283, 1368
StyD4I	CCNGG	7 cut(s)	220, 543, 555, 1136, 1137, 1395, 1628
StyI	CCWWGG	4 cut(s)	678, 1128, 1155, 1661
TaaI	ACNGT	3 cut(s)	916, 1476, 1546
TaiI	ACGT	2 cut(s)	483, 933
TaqI	TCGA	4 cut(s)	185, 255, 332, 1467
TatI	WGTACW	1 cut(s)	1476
TfiI	GAWTC	3 cut(s)	182, 695, 891
Tru1I	TTAA	8 cut(s)	129, 284, 300, 477, 798, 846, 1062, 1356
Tru9I	TTAA	8 cut(s)	129, 284, 300, 477, 798, 846, 1062, 1356
TscAI	CASTG	3 cut(s)	597, 921, 1252
TseFI	GTSAC	2 cut(s)	560, 1315
TseI	GCWGC	9 cut(s)	163, 347, 362, 428, 614, 902, 980, 1133, 1505
Tsp45I	GTSAC	2 cut(s)	560, 1315
TspDTI	ATGAA	6 cut(s)	256, 686, 939, 1284, 1336, 1597
TspGWI	ACGGA	4 cut(s)	97, 474, 1485, 1554
TspMI	CCCGGG	1 cut(s)	1137
TspRI	CASTG	3 cut(s)	597, 921, 1252
Vha464I	CTTAAG	1 cut(s)	128
VpaK11BI	GGWCC	3 cut(s)	120, 1145, 1658
XapI	RAATTY	2 cut(s)	312, 721
XceI	RCATGY	1 cut(s)	86
XmaI	CCCGGG	1 cut(s)	1137
XspI	CTAG	4 cut(s)	534, 1118, 1283, 1368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.