Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007977

Physical Location & Seq

Genomic Coordinates Forward (+)

25976156 .. 25979956

Gene Structure 3801 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1179400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGCGAGTGGTCTCTCACACCACCACCTTCTCTGCATGTATTCCTCAAAAACCCACCCACCGTTTTCAAAAACGCAAACACTTTTCAGAAACCCGAAATCCTCCTCCGTTGCTCTCCGGACCCTCCCGAAGTCGTTGTCTCCGAAAAACTCCAACACTTCGCTGCAAAGTTCGCCGGAAGAGTCGAAGCCCGCCAGCGCTCTTAAGCGGGCCGACCCGCCCGGCCAAAACCCACCTCCCAGATTGTCTTCTTTCGACTCCCTCTTCGTCCGCCTTCGTCATTTGAGAGATGAAAGGTTTAAGCTTGATGGGATTTTTGTGGACATTCTGTCGATTGCATTGCCTGCTGCTCTGGCTCTTGCTGCTGACCCCATTACCTCTTTGGTTGACACAGCCTTTGTTGGGCATTTAGGGTCGGTTGAATTGGCAGCAGTTGGGGTGTCAGCTTCAGTATTCAACTTAGTTTCTAAATTGTTTAACGTTCCATTGCTCAACATCACTACATCTTTTGTTGCTGAAGAGCAGGCATTGGCTAGCAAGGCCGAGGATGATTTGCAGGGTGACAGGCAGAGCAAGAAGCTCCTTCCCTCGGTGTCGACGTCATTGGCTCTTGCTGCCACCTTTGGCGTAGCTGAAGCTGTTGCACTTTTTCTTGGGTCGGGTGTCTTGATGAATGCCATGGGTATATATGCTGATTCACCAATGCGAATACCTGCGGAGAGTTTTCTTGCCGTAAGGGCATTTGGTGCTCCACCGATTGTAATTGCACTTGCCGCACAAGGCACATTTCGTGGCTTTAAAGACACAAAGACGCCTCTGTATGCTGTTGTTGCTGGCAACTTACTTAATGCTATATTGGATGCAATATTGATATTTATTTTTGATTTTGGAATCCGCGGTGCTGCTATTGCTACGGTGATTTCTGAGTACATGATCGCTGTTATTCTTTTGTGGAAATTGAATAGCGAAGTATTACTTGTCCCTACCTATATTGATGGGAGAAGAATCATCGGTTACCTTAAATCTGGGGTCCTTCTTATTGGCAGAAGCTTAGCAGTTGTTGTAACTACGACACTAGCCACATCCGTGGCAGCCCGGGAGGGTCCTATCCCCATGGCTGGCCATCAGATTTGCATTCAAGTTTGGTTGGCAGTATCTTTGCTTACCGATGCTTTAGCACTGGCTGGTCAGACACTTCTCGCCAGTGGTTACTCCCAACAAGATTATGAACAAGCACGCCTAGTAATTTATAGAGTTCTACAGATTGGTTTAGTGACGGGAATTGGTTTGGGAATTATGTTATTCATCGGTTTTAAAGCGTTTTCTAGCTTATTCAGCACAGACCCTCAAGTCCTGGCAATTGCATGGTCTGGTTTATTGTTTGTTGCTGGATCTCAGCCAATAAACGCTCTAGCTTTTGTTATTGATGGACTGTACTACGGAGTTTCAGACTTTGGATTTGCTGCCTATTCAATGGTGTTAGTAGGACTGATTTCTTCCGCGGTCATACTTGTTGCCTCTCCGTCTTTTGGCCTTGCTGGAGTCTGGACCGGGCTGTTTCTCTTCATGGCATTGCGCGTAGTAGCCGGGATTTGGAGGTTGGGCACAAGAACCGGACCATGGAAATTGGTCTGGGATGAGAGGAAACAGGAAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MIF MatE

Protein Analysis

553

Amino Acids

58.77

Weight (kDa)

9.15

Isoelectric Point (pI)

34.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	115 - 288	2.2e-15	MatE
MatE	PF01554	350 - 495	1.3e-11	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38330
fragaria_vesca	FvH4_6g10700 FvH4_6g10700
malus_domestica	MD00G1176800.v1.1 MD04G1165300.v1.1 MD12G1179400.v1.1
prunus_persica	Prupe.6G284300_v2.0.a1
pyrus_communis	pycom04g14730 pycom04g14740 pycom12g16920
rosa_chinensis	RchiOBHm_Chr3g0461701
rosa_laevigata	RLG00000024904
rosa_multiflora	Rmu_co8339943.1_g000001 Rmu_co8373083.1_g000001 Rmu_sc0012406.1_g000005 Rmu_sc0027127.1_g000001 Rmu_sc0038320.1_g000003
rosa_roxburghii	Rroxscaffold_6G00418030
rosa_rugosa	Rorug03G0053100
rosa_samantha	Rh3AG111300 Rh3BG114300 Rh3CG116500 Rh3DG116200
rosa_wichuraiana	Rw3G009420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1349
AatII	GACGTC	1 cut(s)	602
Acc36I	ACCTGC	1 cut(s)	721
AccI	GTMKAC	1 cut(s)	596
AccII	CGCG	3 cut(s)	897, 1502, 1578
AccIII	TCCGGA	1 cut(s)	117
AclI	AACGTT	1 cut(s)	480
AclWI	GGATC	1 cut(s)	1399
AcoI	YGGCCR	2 cut(s)	223, 1120
AcuI	CTGAAG	3 cut(s)	432, 537, 654
AcyI	GRCGYC	2 cut(s)	599, 812
AfaI	GTAC	2 cut(s)	929, 1436
AfeI	AGCGCT	1 cut(s)	199
AfiI	CCNNNNNNNGG	6 cut(s)	402, 589, 1118, 1529, 1593, 1620
AflII	CTTAAG	1 cut(s)	203
AgsI	TTSAA	6 cut(s)	69, 422, 457, 961, 1139, 1473
AhdI	GACNNNNNGTC	1 cut(s)	328
AjnI	CCWGG	1 cut(s)	1353
AleI	CACNNNNGTG	1 cut(s)	1085
AluBI	AGCT	8 cut(s)	304, 446, 580, 632, 638, 1050, 1329, 1415
AluI	AGCT	8 cut(s)	304, 446, 580, 632, 638, 1050, 1329, 1415
Alw21I	GWGCWC	1 cut(s)	751
Alw26I	GTCTC	2 cut(s)	17, 144
AlwI	GGATC	1 cut(s)	1399
Ama87I	CYCGRG	1 cut(s)	1095
Aor13HI	TCCGGA	1 cut(s)	117
Aor51HI	AGCGCT	1 cut(s)	199
AoxI	GGCC	5 cut(s)	210, 223, 540, 1120, 1531
ApeKI	GCWGC	8 cut(s)	163, 347, 362, 428, 614, 902, 1091, 1463
ArsI	GACNNNNNNTTYG	8 cut(s)	122, 154, 248, 280, 380, 412, 1442, 1474
AspLEI	GCGC	2 cut(s)	200, 1578
AspS9I	GGNCC	6 cut(s)	120, 210, 1030, 1103, 1548, 1616
AsuC2I	CCSGG	5 cut(s)	222, 1096, 1097, 1552, 1588
AsuHPI	GGTGA	3 cut(s)	572, 690, 928
AsuNHI	GCTAGC	1 cut(s)	533
AvaI	CYCGRG	1 cut(s)	1095
AvaII	GGWCC	5 cut(s)	120, 1030, 1103, 1548, 1616
BaeGI	GKGCMC	1 cut(s)	1607
BalI	TGGCCA	1 cut(s)	1122
BbsI	GAAGAC	1 cut(s)	240
Bbv12I	GWGCWC	1 cut(s)	751
BbvI	GCAGC	8 cut(s)	150, 334, 349, 440, 601, 889, 1103, 1450
BccI	CCATC	4 cut(s)	302, 989, 1131, 1421
BceAI	ACGGC	1 cut(s)	716
BcgI	CGANNNNNNTGC	2 cut(s)	322, 356
BciT130I	CCWGG	1 cut(s)	1355
BcnI	CCSGG	5 cut(s)	222, 1096, 1097, 1552, 1588
BcoDI	GTCTC	2 cut(s)	17, 144
BfaI	CTAG	5 cut(s)	534, 1076, 1241, 1326, 1412
BfmI	CTRYAG	1 cut(s)	1259
BfoI	RGCGCY	1 cut(s)	201
BfrI	CTTAAG	1 cut(s)	203
BfuAI	ACCTGC	1 cut(s)	721
BglI	GCCNNNNNGGC	1 cut(s)	737
BisI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 774, 903, 1092, 1464
BlpI	GCTNAGC	1 cut(s)	1051
BlsI	GCNGC	9 cut(s)	165, 349, 364, 430, 616, 775, 904, 1093, 1465
Bme1390I	CCNGG	6 cut(s)	222, 1096, 1097, 1355, 1552, 1588
Bme18I	GGWCC	5 cut(s)	120, 1030, 1103, 1548, 1616
BmeRI	GACNNNNNGTC	1 cut(s)	328
BmeT110I	CYCGRG	1 cut(s)	1095
BmgT120I	GGNCC	6 cut(s)	120, 210, 1030, 1103, 1548, 1616
BmiI	GGNNCC	3 cut(s)	122, 1031, 1104
BmrFI	CCNGG	6 cut(s)	222, 1096, 1097, 1355, 1552, 1588
BmsI	GCATC	2 cut(s)	850, 1159
BmtI	GCTAGC	1 cut(s)	537
BpiI	GAAGAC	1 cut(s)	240
BpmI	CTGGAG	1 cut(s)	1560
Bpu1102I	GCTNAGC	1 cut(s)	1051
BpuEI	CTTGAG	1 cut(s)	1332
BpuMI	CCSGG	5 cut(s)	222, 1096, 1097, 1552, 1588
BsaHI	GRCGYC	2 cut(s)	599, 812
BsaI	GGTCTC	1 cut(s)	17
BsaJI	CCNNGG	9 cut(s)	543, 588, 678, 895, 1086, 1095, 1113, 1500, 1619
BsaWI	WCCGGW	2 cut(s)	117, 1613
Bsc4I	CCNNNNNNNGG	6 cut(s)	402, 589, 1118, 1529, 1593, 1620
Bse1I	ACTGG	2 cut(s)	1185, 1203
Bse3DI	GCAATG	3 cut(s)	338, 485, 1571
BseAI	TCCGGA	1 cut(s)	117
BseBI	CCWGG	1 cut(s)	1355
BseDI	CCNNGG	9 cut(s)	543, 588, 678, 895, 1086, 1095, 1113, 1500, 1619
BseGI	GGATG	4 cut(s)	553, 865, 1082, 1642
BseLI	CCNNNNNNNGG	6 cut(s)	402, 589, 1118, 1529, 1593, 1620
BseMI	GCAATG	3 cut(s)	338, 485, 1571
BseMII	CTCAG	2 cut(s)	915, 1409
BseNI	ACTGG	2 cut(s)	1185, 1203
BseRI	GAGGAG	1 cut(s)	94
BseSI	GKGCMC	1 cut(s)	1607
BseXI	GCAGC	8 cut(s)	150, 334, 349, 440, 601, 889, 1103, 1450
Bsh1236I	CGCG	3 cut(s)	897, 1502, 1578
BshFI	GGCC	5 cut(s)	212, 225, 542, 1122, 1533
BsiHKAI	GWGCWC	1 cut(s)	751
BsiHKCI	CYCGRG	1 cut(s)	1095
BsiSI	CCGG	7 cut(s)	118, 176, 222, 1096, 1551, 1587, 1614
BslFI	GGGAC	1 cut(s)	965
BslI	CCNNNNNNNGG	6 cut(s)	402, 589, 1118, 1529, 1593, 1620
BsmAI	GTCTC	2 cut(s)	17, 144
BsmFI	GGGAC	1 cut(s)	965
BsmI	GAATGC	2 cut(s)	679, 1134
BsnI	GGCC	5 cut(s)	212, 225, 542, 1122, 1533
Bso31I	GGTCTC	1 cut(s)	17
BsoBI	CYCGRG	1 cut(s)	1095
Bsp1286I	GDGCHC	2 cut(s)	751, 1607
Bsp13I	TCCGGA	1 cut(s)	117
Bsp143I	GATC	2 cut(s)	933, 1391
Bsp1720I	GCTNAGC	1 cut(s)	1051
Bsp19I	CCATGG	3 cut(s)	678, 1113, 1619
BspANI	GGCC	5 cut(s)	212, 225, 542, 1122, 1533
BspCNI	CTCAG	2 cut(s)	916, 1408
BspEI	TCCGGA	1 cut(s)	117
BspFNI	CGCG	3 cut(s)	897, 1502, 1578
BspLI	GGNNCC	3 cut(s)	122, 1031, 1104
BspMI	ACCTGC	1 cut(s)	721
BspOI	GCTAGC	1 cut(s)	537
BspPI	GGATC	1 cut(s)	1399
BspQI	GCTCTTC	1 cut(s)	513
BspTI	CTTAAG	1 cut(s)	203
BspTNI	GGTCTC	1 cut(s)	17
BsrDI	GCAATG	3 cut(s)	338, 485, 1571
BsrI	ACTGG	2 cut(s)	1185, 1203
BssECI	CCNNGG	9 cut(s)	543, 588, 678, 895, 1086, 1095, 1113, 1500, 1619
BssMI	GATC	2 cut(s)	933, 1391
BssNI	GRCGYC	2 cut(s)	599, 812
BssT1I	CCWWGG	3 cut(s)	678, 1113, 1619
Bst2UI	CCWGG	1 cut(s)	1355
Bst4CI	ACNGT	3 cut(s)	63, 916, 1434
Bst6I	CTCTTC	4 cut(s)	174, 269, 513, 1568
BstACI	GRCGYC	2 cut(s)	599, 812
BstAFI	CTTAAG	1 cut(s)	203
BstAPI	GCANNNNNTGC	2 cut(s)	344, 746
BstC8I	GCNNGC	9 cut(s)	192, 196, 210, 345, 525, 535, 835, 1120, 1237
BstDEI	CTNAG	4 cut(s)	460, 924, 1051, 1395
BstDSI	CCRYGG	6 cut(s)	678, 895, 1086, 1113, 1500, 1619
BstEII	GGTNACC	1 cut(s)	1013
BstF5I	GGATG	4 cut(s)	553, 865, 1082, 1642
BstFNI	CGCG	3 cut(s)	897, 1502, 1578
BstH2I	RGCGCY	1 cut(s)	201
BstHHI	GCGC	2 cut(s)	200, 1578
BstKTI	GATC	2 cut(s)	936, 1394
BstMAI	GTCTC	2 cut(s)	17, 144
BstMBI	GATC	2 cut(s)	933, 1391
BstNI	CCWGG	1 cut(s)	1355
BstNSI	RCATGY	1 cut(s)	40
BstPI	GGTNACC	1 cut(s)	1013
BstSCI	CCNGG	6 cut(s)	220, 1094, 1095, 1353, 1550, 1586
BstSFI	CTRYAG	1 cut(s)	1259
BstSLI	GKGCMC	1 cut(s)	1607
BstUI	CGCG	3 cut(s)	897, 1502, 1578
BstV1I	GCAGC	8 cut(s)	150, 334, 349, 440, 601, 889, 1103, 1450
BstV2I	GAAGAC	1 cut(s)	240
BstX2I	RGATCY	1 cut(s)	1391
BstXI	CCANNNNNNTGG	1 cut(s)	1087
BstYI	RGATCY	1 cut(s)	1391
BsuRI	GGCC	5 cut(s)	212, 225, 542, 1122, 1533
BtgI	CCRYGG	6 cut(s)	678, 895, 1086, 1113, 1500, 1619
BtsCI	GGATG	4 cut(s)	553, 865, 1082, 1642
BtsIMutI	CAGTG	2 cut(s)	1178, 1210
BveI	ACCTGC	1 cut(s)	721
Cac8I	GCNNGC	9 cut(s)	192, 196, 210, 345, 525, 535, 835, 1120, 1237
CfoI	GCGC	2 cut(s)	200, 1578
Cfr13I	GGNCC	6 cut(s)	120, 210, 1030, 1103, 1548, 1616
Cfr42I	CCGCGG	2 cut(s)	898, 1503
Cfr9I	CCCGGG	1 cut(s)	1095
CseI	GACGC	1 cut(s)	820
Csp6I	GTAC	2 cut(s)	928, 1435
CviAII	CATG	7 cut(s)	37, 679, 931, 1114, 1365, 1567, 1620
CviQI	GTAC	2 cut(s)	928, 1435
DdeI	CTNAG	4 cut(s)	460, 924, 1051, 1395
DpnI	GATC	2 cut(s)	935, 1393
DpnII	GATC	2 cut(s)	933, 1391
DraI	TTTAAA	2 cut(s)	799, 1315
DrdI	GACNNNNNNGTC	1 cut(s)	1349
DriI	GACNNNNNGTC	1 cut(s)	328
DseDI	GACNNNNNNGTC	1 cut(s)	1349
EaeI	YGGCCR	2 cut(s)	223, 1120
Eam1104I	CTCTTC	4 cut(s)	174, 269, 513, 1568
Eam1105I	GACNNNNNGTC	1 cut(s)	328
EarI	CTCTTC	4 cut(s)	174, 269, 513, 1568
EciI	GGCGGA	1 cut(s)	260
Eco130I	CCWWGG	3 cut(s)	678, 1113, 1619
Eco31I	GGTCTC	1 cut(s)	17
Eco47I	GGWCC	5 cut(s)	120, 1030, 1103, 1548, 1616
Eco47III	AGCGCT	1 cut(s)	199
Eco57I	CTGAAG	3 cut(s)	432, 537, 654
Eco88I	CYCGRG	1 cut(s)	1095
Eco91I	GGTNACC	1 cut(s)	1013
EcoO109I	RGGNCCY	2 cut(s)	1030, 1103
EcoO65I	GGTNACC	1 cut(s)	1013
EcoRII	CCWGG	1 cut(s)	1353
EcoT14I	CCWWGG	3 cut(s)	678, 1113, 1619
ErhI	CCWWGG	3 cut(s)	678, 1113, 1619
FaeI	CATG	7 cut(s)	40, 682, 934, 1117, 1368, 1570, 1623
FalI	AAGNNNNNCTT	2 cut(s)	960, 992
FaqI	GGGAC	1 cut(s)	965
FatI	CATG	7 cut(s)	36, 678, 930, 1113, 1364, 1566, 1619
FauI	CCCGC	3 cut(s)	199, 201, 225
FblI	GTMKAC	1 cut(s)	596
Fnu4HI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 774, 903, 1092, 1464
FokI	GGATG	4 cut(s)	560, 872, 1069, 1649
Fsp4HI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 774, 903, 1092, 1464
FspBI	CTAG	5 cut(s)	534, 1076, 1241, 1326, 1412
GlaI	GCGC	2 cut(s)	199, 1577
GluI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 774, 903, 1092, 1464
GsuI	CTGGAG	1 cut(s)	1560
HaeII	RGCGCY	1 cut(s)	201
HaeIII	GGCC	5 cut(s)	212, 225, 542, 1122, 1533
HapII	CCGG	7 cut(s)	118, 176, 222, 1096, 1551, 1587, 1614
HgaI	GACGC	1 cut(s)	820
HhaI	GCGC	2 cut(s)	200, 1578
Hin1I	GRCGYC	2 cut(s)	599, 812
Hin1II	CATG	7 cut(s)	40, 682, 934, 1117, 1368, 1570, 1623
Hin6I	GCGC	2 cut(s)	198, 1576
HinP1I	GCGC	2 cut(s)	198, 1576
HincII	GTYRAC	2 cut(s)	388, 597
HindII	GTYRAC	2 cut(s)	388, 597
HindIII	AAGCTT	2 cut(s)	302, 1048
HinfI	GANTC	6 cut(s)	182, 257, 695, 891, 1005, 1542
HpaII	CCGG	7 cut(s)	118, 176, 222, 1096, 1551, 1587, 1614
HphI	GGTGA	3 cut(s)	572, 690, 928
Hpy166II	GTNNAC	3 cut(s)	322, 388, 597
Hpy188I	TCNGA	6 cut(s)	89, 144, 925, 1128, 1191, 1450
Hpy188III	TCNNGA	4 cut(s)	118, 127, 667, 1546
Hpy8I	GTNNAC	3 cut(s)	322, 388, 597
Hpy99I	CGWCG	1 cut(s)	601
HpyAV	CCTTC	5 cut(s)	38, 284, 593, 1043, 1646
HpyCH4III	ACNGT	3 cut(s)	63, 916, 1434
HpyCH4IV	ACGT	2 cut(s)	480, 599
HpyCH4V	TGCA	9 cut(s)	36, 166, 338, 556, 644, 767, 863, 1134, 1364
HpyF3I	CTNAG	4 cut(s)	460, 924, 1051, 1395
HpySE526I	ACGT	2 cut(s)	480, 599
Hsp92I	GRCGYC	2 cut(s)	599, 812
Hsp92II	CATG	7 cut(s)	40, 682, 934, 1117, 1368, 1570, 1623
HspAI	GCGC	2 cut(s)	198, 1576
Kpn2I	TCCGGA	1 cut(s)	117
KspI	CCGCGG	2 cut(s)	898, 1503
Kzo9I	GATC	2 cut(s)	933, 1391
LguI	GCTCTTC	1 cut(s)	513
LmnI	GCTCC	2 cut(s)	585, 754
Lsp1109I	GCAGC	8 cut(s)	150, 334, 349, 440, 601, 889, 1103, 1450
LweI	GCATC	2 cut(s)	850, 1159
MaeI	CTAG	5 cut(s)	534, 1076, 1241, 1326, 1412
MaeII	ACGT	2 cut(s)	480, 599
MaeIII	GTNAC	5 cut(s)	560, 1013, 1063, 1208, 1273
MalI	GATC	2 cut(s)	935, 1393
MboI	GATC	2 cut(s)	933, 1391
MboII	GAAGA	7 cut(s)	191, 240, 256, 530, 1014, 1488, 1555
MfeI	CAATTG	1 cut(s)	1359
MflI	RGATCY	1 cut(s)	1391
MhlI	GDGCHC	2 cut(s)	751, 1607
MlsI	TGGCCA	1 cut(s)	1122
MluCI	AATT	9 cut(s)	422, 470, 762, 956, 1245, 1281, 1293, 1359, 1625
MluNI	TGGCCA	1 cut(s)	1122
MlyI	GAGTC	3 cut(s)	191, 251, 1551
MmeI	TCCRAC	1 cut(s)	177
Mox20I	TGGCCA	1 cut(s)	1122
MroI	TCCGGA	1 cut(s)	117
MscI	TGGCCA	1 cut(s)	1122
MseI	TTAA	8 cut(s)	204, 300, 477, 798, 846, 1020, 1314, 1657
MslI	CAYNNNNRTG	1 cut(s)	1085
Msp20I	TGGCCA	1 cut(s)	1122
MspA1I	CMGCKG	2 cut(s)	897, 1502
MspCI	CTTAAG	1 cut(s)	203
MspI	CCGG	7 cut(s)	118, 176, 222, 1096, 1551, 1587, 1614
MspR9I	CCNGG	6 cut(s)	222, 1096, 1097, 1355, 1552, 1588
MunI	CAATTG	1 cut(s)	1359
Mva1269I	GAATGC	2 cut(s)	679, 1134
MvaI	CCWGG	1 cut(s)	1355
MvnI	CGCG	3 cut(s)	897, 1502, 1578
NciI	CCSGG	5 cut(s)	222, 1096, 1097, 1552, 1588
NcoI	CCATGG	3 cut(s)	678, 1113, 1619
NdeII	GATC	2 cut(s)	933, 1391
NheI	GCTAGC	1 cut(s)	533
NlaIII	CATG	7 cut(s)	40, 682, 934, 1117, 1368, 1570, 1623
NlaIV	GGNNCC	3 cut(s)	122, 1031, 1104
NmeAIII	GCCGAG	1 cut(s)	568
NmuCI	GTSAC	2 cut(s)	560, 1273
NspI	RCATGY	1 cut(s)	40
OliI	CACNNNNGTG	1 cut(s)	1085
PciSI	GCTCTTC	1 cut(s)	513
PcsI	WCGNNNNNNNCGW	2 cut(s)	140, 596
PctI	GAATGC	2 cut(s)	679, 1134
PfeI	GAWTC	3 cut(s)	695, 891, 1005
PkrI	GCNGC	9 cut(s)	165, 349, 364, 430, 616, 775, 904, 1093, 1465
PleI	GAGTC	3 cut(s)	190, 251, 1550
PpsI	GAGTC	3 cut(s)	190, 251, 1550
PpuMI	RGGWCCY	2 cut(s)	1030, 1103
Psp1406I	AACGTT	1 cut(s)	480
Psp5II	RGGWCCY	2 cut(s)	1030, 1103
Psp6I	CCWGG	1 cut(s)	1353
PspEI	GGTNACC	1 cut(s)	1013
PspGI	CCWGG	1 cut(s)	1353
PspN4I	GGNNCC	3 cut(s)	122, 1031, 1104
PspPI	GGNCC	6 cut(s)	120, 210, 1030, 1103, 1548, 1616
PspPPI	RGGWCCY	2 cut(s)	1030, 1103
PsuI	RGATCY	1 cut(s)	1391
RsaI	GTAC	2 cut(s)	929, 1436
RsaNI	GTAC	2 cut(s)	928, 1435
RseI	CAYNNNNRTG	1 cut(s)	1085
SacII	CCGCGG	2 cut(s)	898, 1503
SalI	GTCGAC	1 cut(s)	595
SapI	GCTCTTC	1 cut(s)	513
SaqAI	TTAA	8 cut(s)	204, 300, 477, 798, 846, 1020, 1314, 1657
SatI	GCNGC	9 cut(s)	164, 348, 363, 429, 615, 774, 903, 1092, 1464
Sau3AI	GATC	2 cut(s)	933, 1391
Sau96I	GGNCC	6 cut(s)	120, 210, 1030, 1103, 1548, 1616
SchI	GAGTC	3 cut(s)	191, 251, 1551
ScrFI	CCNGG	6 cut(s)	222, 1096, 1097, 1355, 1552, 1588
SduI	GDGCHC	2 cut(s)	751, 1607
SfaNI	GCATC	2 cut(s)	850, 1159
SfcI	CTRYAG	1 cut(s)	1259
Sfr303I	CCGCGG	2 cut(s)	898, 1503
SgrBI	CCGCGG	2 cut(s)	898, 1503
SinI	GGWCC	5 cut(s)	120, 1030, 1103, 1548, 1616
SmaI	CCCGGG	1 cut(s)	1097
SmiMI	CAYNNNNRTG	1 cut(s)	1085
SmlI	CTYRAG	2 cut(s)	203, 1347
SmoI	CTYRAG	2 cut(s)	203, 1347
Sse9I	AATT	9 cut(s)	422, 470, 762, 956, 1245, 1281, 1293, 1359, 1625
SspI	AATATT	1 cut(s)	867
SspMI	CTAG	5 cut(s)	534, 1076, 1241, 1326, 1412
StyD4I	CCNGG	6 cut(s)	220, 1094, 1095, 1353, 1550, 1586
StyI	CCWWGG	3 cut(s)	678, 1113, 1619
TaaI	ACNGT	3 cut(s)	63, 916, 1434
TaiI	ACGT	2 cut(s)	483, 602
TaqI	TCGA	4 cut(s)	185, 255, 332, 596
TasI	AATT	9 cut(s)	422, 470, 762, 956, 1245, 1281, 1293, 1359, 1625
TatI	WGTACW	2 cut(s)	927, 1434
TauI	GCSGC	1 cut(s)	776
TfiI	GAWTC	3 cut(s)	695, 891, 1005
Tru1I	TTAA	8 cut(s)	204, 300, 477, 798, 846, 1020, 1314, 1657
Tru9I	TTAA	8 cut(s)	204, 300, 477, 798, 846, 1020, 1314, 1657
TscAI	CASTG	2 cut(s)	1185, 1210
TseFI	GTSAC	2 cut(s)	560, 1273
TseI	GCWGC	8 cut(s)	163, 347, 362, 428, 614, 902, 1091, 1463
Tsp45I	GTSAC	2 cut(s)	560, 1273
TspDTI	ATGAA	5 cut(s)	306, 686, 1242, 1294, 1555
TspGWI	ACGGA	4 cut(s)	97, 1075, 1455, 1512
TspMI	CCCGGG	1 cut(s)	1095
TspRI	CASTG	2 cut(s)	1185, 1210
Vha464I	CTTAAG	1 cut(s)	203
VpaK11BI	GGWCC	5 cut(s)	120, 1030, 1103, 1548, 1616
XceI	RCATGY	1 cut(s)	40
XcmI	CCANNNNNNNNNTGG	1 cut(s)	379
XmaI	CCCGGG	1 cut(s)	1095
XmiI	GTMKAC	1 cut(s)	596
XspI	CTAG	5 cut(s)	534, 1076, 1241, 1326, 1412
ZraI	GACGTC	1 cut(s)	600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.