Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004728

Physical Location & Seq

Genomic Coordinates Forward (+)

43428176 .. 43433128

Gene Structure 4953 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1184200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1032 bp

ATGCCCTCCAACTTCCTTACTAATTCCACGTCCACCCCAACACCCGACCCCTCCGATTCCGCCTCCTCATCCTCCGACGCTCAGTCCGCCGCAGCGTCCCCAAAGACGACACCCGCAGAGATGTGGAGCTACCTCTGGATTCCTTTCCTAATTTCCCTGTCCAAGGAGCTGAGCTTGGCCAAAGCCCAGTCTACGATTGTCTTACCAAGTCAACTCGGAACATCCGACTCGTCCTCGACCAAATGTGCGGCGCCCGACCCCAAGCTCAATTACCGGCCCGTGATCGGCATCCTGAGCCACCCCGGAGACGGCGCCTCCGGCCGGCTCAGCAACGCCTCCACGGCGTCGTATATCGCAGCTTCATATGTCAAGTTCGTCGAATCGGCTGGGGCTCGTGTTATTCCTCTCATCTATAACGAGCCGCGTGATATTCTTTTCCAGAAGCTCGATCTCGTTAATGGCGTGCTTTTCACCGGAGGATGGGCTAAACGTGGGTTGTACTATGATGTTGTTGAGAGAATATTTCAGAAAATTATAGAGAAGAATGACGCTGGAGATCATTTCCCATTATACGCCATCTGCTTAGGTTTTGAACTTCTAACAATGATCATCAGCAAGAACAAGAAAATTCTTGAGTCATTCAGTGCAGTAGATGTGGCGTCAACTTTACAATTTACCGAGAACACCATTATTGAAGGAACCGTATTTCAAAGATTTCCTCCAGACTTGCTTAGGAAGTTGAGTACAGATTGTCTTGTAATGCAAAACCATAAATATGGTATTTCACCAGAGAGGCTTATCGAGAACGAGAATCTGTCAAGTTTTTTTAAGATCTTGACAACTTGTACTGATGAAGATAATAAGGTCTATGTGTCCACAGCACACGCATACAACTATCCTGTGACCGCCTTCCAATGGCATCCAGAGAAAAATGCTTTTGAATGGGGATTACCAATGATTCCGCACTCAGAGGATGCTATTCAAGTGACCCAGCACGTCGCAAACTTCTTGGTCAGGCAAGGAAGTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006082 GO:0006508 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008242 GO:0009987 GO:0016787 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034722 GO:0042558 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044281 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046900 GO:0051186 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00790 ko01523 map00790 map01523

Pfam Domains

Protein Families

Peptidase_C26

Protein Analysis

344

Amino Acids

37.83

Weight (kDa)

5.77

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C26	PF07722	93 - 309	6.9e-11	Peptidase C26
GATase	PF00117	126 - 313	1.9e-09	Glutamine amidotransferase class-I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78660 AT1G78660 AT1G78660 AT1G78680 AT1G78680
fragaria_vesca	FvH4_3g18102 FvH4_3g18102 FvH4_3g18102 FvH4_3g18102 FvH4_3g18102 FvH4_3g18102 FvH4_3g18102
malus_domestica	MD00G1184200.v1.1 MD03G1244700.v1.1 MD03G1244800.v1.1
prunus_persica	Prupe.4G159900_v2.0.a1
pyrus_communis	pycom03g19410 pycom11g23430
rosa_chinensis	RchiOBHm_Chr5g0030171 RchiOBHm_Chr5g0030241 RchiOBHm_Chr5g0030431 RchiOBHm_Chr5g0030511
rosa_laevigata	RLG00000033221
rosa_multiflora	Rmu_sc0000280.1_g000003 Rmu_sc0015917.1_g000002
rosa_roxburghii	Rroxscaffold_1G00049790
rosa_rugosa	Rorug05G0118100
rosa_samantha	Rh5AG210300 Rh5BG209500 Rh5DG213700
rosa_wichuraiana	Rw5G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	250, 311
AccI	GTMKAC	1 cut(s)	191
AccII	CGCG	1 cut(s)	424
AciI	CCGC	8 cut(s)	60, 87, 90, 114, 248, 422, 906, 962
AcoI	YGGCCR	2 cut(s)	177, 319
AcsI	RAATTY	1 cut(s)	627
AcyI	GRCGYC	4 cut(s)	251, 312, 344, 659
AfaI	GTAC	3 cut(s)	500, 745, 847
AfiI	CCNNNNNNNGG	6 cut(s)	163, 284, 308, 321, 480, 915
AgsI	TTSAA	5 cut(s)	593, 695, 710, 941, 983
AhdI	GACNNNNNGTC	1 cut(s)	82
AjiI	CACGTC	2 cut(s)	30, 997
AluBI	AGCT	6 cut(s)	129, 169, 174, 265, 359, 445
AluI	AGCT	6 cut(s)	129, 169, 174, 265, 359, 445
Alw26I	GTCTC	1 cut(s)	300
AoxI	GGCC	3 cut(s)	177, 275, 319
ApeKI	GCWGC	2 cut(s)	92, 356
ApoI	RAATTY	1 cut(s)	627
AspLEI	GCGC	2 cut(s)	253, 314
AspS9I	GGNCC	1 cut(s)	276
AsuC2I	CCSGG	1 cut(s)	303
AsuHPI	GGTGA	2 cut(s)	463, 777
BalI	TGGCCA	1 cut(s)	179
BanI	GGYRCC	2 cut(s)	250, 311
BanII	GRGCYC	1 cut(s)	394
BauI	CACGAG	1 cut(s)	393
BbvI	GCAGC	2 cut(s)	104, 368
BccI	CCATC	2 cut(s)	474, 584
BceAI	ACGGC	2 cut(s)	325, 357
BclI	TGATCA	1 cut(s)	606
BcnI	CCSGG	1 cut(s)	303
BcoDI	GTCTC	1 cut(s)	300
BfoI	RGCGCY	2 cut(s)	254, 315
BglI	GCCNNNNNGGC	1 cut(s)	341
BglII	AGATCT	1 cut(s)	831
BisI	GCNGC	5 cut(s)	90, 93, 249, 357, 422
BlpI	GCTNAGC	2 cut(s)	170, 326
BlsI	GCNGC	5 cut(s)	91, 94, 250, 358, 423
Bme1390I	CCNGG	1 cut(s)	303
BmeRI	GACNNNNNGTC	1 cut(s)	82
BmgBI	CACGTC	2 cut(s)	30, 997
BmgT120I	GGNCC	1 cut(s)	276
BmiI	GGNNCC	3 cut(s)	252, 313, 700
BmrFI	CCNGG	1 cut(s)	303
BmrI	ACTGGG	1 cut(s)	181
BmsI	GCATC	3 cut(s)	297, 928, 964
BmuI	ACTGGG	1 cut(s)	181
BpmI	CTGGAG	2 cut(s)	573, 705
Bpu10I	CCTNAGC	3 cut(s)	293, 583, 731
Bpu1102I	GCTNAGC	2 cut(s)	170, 326
BpuEI	CTTGAG	1 cut(s)	653
BpuMI	CCSGG	1 cut(s)	303
BsaBI	GATNNNNATC	1 cut(s)	287
BsaHI	GRCGYC	4 cut(s)	251, 312, 344, 659
BsaJI	CCNNGG	3 cut(s)	162, 301, 339
BsaWI	WCCGGW	1 cut(s)	473
BsaXI	ACNNNNNCTCC	2 cut(s)	299, 329
Bsc4I	CCNNNNNNNGG	6 cut(s)	163, 284, 308, 321, 480, 915
Bse118I	RCCGGY	2 cut(s)	273, 321
Bse1I	ACTGG	1 cut(s)	187
Bse8I	GATNNNNATC	1 cut(s)	287
BseDI	CCNNGG	3 cut(s)	162, 301, 339
BseGI	GGATG	6 cut(s)	68, 221, 288, 485, 919, 979
BseJI	GATNNNNATC	1 cut(s)	287
BseLI	CCNNNNNNNGG	6 cut(s)	163, 284, 308, 321, 480, 915
BseMII	CTCAG	5 cut(s)	95, 161, 284, 340, 981
BseNI	ACTGG	1 cut(s)	187
BseRI	GAGGAG	1 cut(s)	55
BseX3I	CGGCCG	1 cut(s)	319
BseXI	GCAGC	2 cut(s)	104, 368
BseYI	CCCAGC	2 cut(s)	386, 990
BsgI	GTGCAG	1 cut(s)	666
Bsh1236I	CGCG	1 cut(s)	424
Bsh1285I	CGRYCG	1 cut(s)	322
BshFI	GGCC	3 cut(s)	179, 277, 321
BshNI	GGYRCC	2 cut(s)	250, 311
BsiEI	CGRYCG	1 cut(s)	322
BsiSI	CCGG	5 cut(s)	274, 303, 318, 322, 474
BslFI	GGGAC	1 cut(s)	82
BslI	CCNNNNNNNGG	6 cut(s)	163, 284, 308, 321, 480, 915
BsmAI	GTCTC	1 cut(s)	300
BsmBI	CGTCTC	1 cut(s)	300
BsmFI	GGGAC	1 cut(s)	82
BsnI	GGCC	3 cut(s)	179, 277, 321
Bsp1286I	GDGCHC	1 cut(s)	394
Bsp143I	GATC	5 cut(s)	282, 448, 556, 606, 831
Bsp1720I	GCTNAGC	2 cut(s)	170, 326
BspACI	CCGC	8 cut(s)	60, 87, 90, 114, 248, 422, 906, 962
BspANI	GGCC	3 cut(s)	179, 277, 321
BspCNI	CTCAG	5 cut(s)	94, 162, 285, 339, 980
BspFNI	CGCG	1 cut(s)	424
BspLI	GGNNCC	3 cut(s)	252, 313, 700
BspT107I	GGYRCC	2 cut(s)	250, 311
BsrFI	RCCGGY	2 cut(s)	273, 321
BsrI	ACTGG	1 cut(s)	187
BssAI	RCCGGY	2 cut(s)	273, 321
BssECI	CCNNGG	3 cut(s)	162, 301, 339
BssMI	GATC	5 cut(s)	282, 448, 556, 606, 831
BssNI	GRCGYC	4 cut(s)	251, 312, 344, 659
BssSI	CACGAG	1 cut(s)	393
BssT1I	CCWWGG	1 cut(s)	162
Bst2BI	CACGAG	1 cut(s)	393
Bst4CI	ACNGT	1 cut(s)	703
BstACI	GRCGYC	4 cut(s)	251, 312, 344, 659
BstC8I	GCNNGC	2 cut(s)	323, 464
BstDEI	CTNAG	7 cut(s)	81, 170, 293, 326, 583, 731, 967
BstDSI	CCRYGG	1 cut(s)	339
BstENI	CCTNNNNNAGG	1 cut(s)	161
BstF5I	GGATG	6 cut(s)	68, 221, 288, 485, 919, 979
BstFNI	CGCG	1 cut(s)	424
BstH2I	RGCGCY	2 cut(s)	254, 315
BstHHI	GCGC	2 cut(s)	253, 314
BstKTI	GATC	5 cut(s)	285, 451, 559, 609, 834
BstMAI	GTCTC	1 cut(s)	300
BstMBI	GATC	5 cut(s)	282, 448, 556, 606, 831
BstMCI	CGRYCG	1 cut(s)	322
BstMWI	GCNNNNNNNGC	5 cut(s)	86, 294, 318, 327, 341
BstSCI	CCNGG	1 cut(s)	301
BstUI	CGCG	1 cut(s)	424
BstV1I	GCAGC	2 cut(s)	104, 368
BstX2I	RGATCY	1 cut(s)	831
BstXI	CCANNNNNNTGG	1 cut(s)	776
BstYI	RGATCY	1 cut(s)	831
BstZI	CGGCCG	1 cut(s)	319
BsuRI	GGCC	3 cut(s)	179, 277, 321
BtgI	CCRYGG	1 cut(s)	339
BtrI	CACGTC	2 cut(s)	30, 997
BtsCI	GGATG	6 cut(s)	68, 221, 288, 485, 919, 979
BtsIMutI	CAGTG	1 cut(s)	649
Cac8I	GCNNGC	2 cut(s)	323, 464
CfoI	GCGC	2 cut(s)	253, 314
Cfr10I	RCCGGY	2 cut(s)	273, 321
Cfr13I	GGNCC	1 cut(s)	276
CseI	GACGC	5 cut(s)	84, 86, 333, 557, 648
Csp6I	GTAC	3 cut(s)	499, 744, 846
CviQI	GTAC	3 cut(s)	499, 744, 846
DdeI	CTNAG	7 cut(s)	81, 170, 293, 326, 583, 731, 967
DinI	GGCGCC	2 cut(s)	252, 313
DpnI	GATC	5 cut(s)	284, 450, 558, 608, 833
DpnII	GATC	5 cut(s)	282, 448, 556, 606, 831
DriI	GACNNNNNGTC	1 cut(s)	82
EaeI	YGGCCR	2 cut(s)	177, 319
EagI	CGGCCG	1 cut(s)	319
Eam1105I	GACNNNNNGTC	1 cut(s)	82
EciI	GGCGGA	2 cut(s)	49, 76
EclXI	CGGCCG	1 cut(s)	319
Eco130I	CCWWGG	1 cut(s)	162
Eco24I	GRGCYC	1 cut(s)	394
Eco52I	CGGCCG	1 cut(s)	319
EcoNI	CCTNNNNNAGG	1 cut(s)	161
EcoT14I	CCWWGG	1 cut(s)	162
EcoT38I	GRGCYC	1 cut(s)	394
EgeI	GGCGCC	2 cut(s)	252, 313
EheI	GGCGCC	2 cut(s)	252, 313
ErhI	CCWWGG	1 cut(s)	162
Esp3I	CGTCTC	1 cut(s)	300
FaqI	GGGAC	1 cut(s)	82
FauI	CCCGC	1 cut(s)	121
FauNDI	CATATG	1 cut(s)	364
FbaI	TGATCA	1 cut(s)	606
FblI	GTMKAC	1 cut(s)	191
Fnu4HI	GCNGC	5 cut(s)	90, 93, 249, 357, 422
FokI	GGATG	6 cut(s)	55, 208, 275, 492, 906, 986
FriOI	GRGCYC	1 cut(s)	394
Fsp4HI	GCNGC	5 cut(s)	90, 93, 249, 357, 422
GlaI	GCGC	2 cut(s)	252, 313
GluI	GCNGC	5 cut(s)	90, 93, 249, 357, 422
GsaI	CCCAGC	2 cut(s)	390, 994
GsuI	CTGGAG	2 cut(s)	573, 705
HaeII	RGCGCY	2 cut(s)	254, 315
HaeIII	GGCC	3 cut(s)	179, 277, 321
HapII	CCGG	5 cut(s)	274, 303, 318, 322, 474
HgaI	GACGC	5 cut(s)	84, 86, 333, 557, 648
HhaI	GCGC	2 cut(s)	253, 314
Hin1I	GRCGYC	4 cut(s)	251, 312, 344, 659
Hin6I	GCGC	2 cut(s)	251, 312
HinP1I	GCGC	2 cut(s)	251, 312
HincII	GTYRAC	2 cut(s)	212, 663
HindII	GTYRAC	2 cut(s)	212, 663
HinfI	GANTC	7 cut(s)	56, 139, 227, 380, 635, 811, 958
HpaII	CCGG	5 cut(s)	274, 303, 318, 322, 474
HphI	GGTGA	2 cut(s)	463, 777
Hpy166II	GTNNAC	5 cut(s)	33, 192, 212, 663, 876
Hpy188I	TCNGA	6 cut(s)	55, 76, 218, 226, 528, 970
Hpy188III	TCNNGA	8 cut(s)	136, 292, 439, 632, 722, 802, 835, 923
Hpy8I	GTNNAC	5 cut(s)	33, 192, 212, 663, 876
Hpy99I	CGWCG	4 cut(s)	80, 349, 380, 1001
HpyAV	CCTTC	2 cut(s)	689, 919
HpyCH4III	ACNGT	1 cut(s)	703
HpyCH4IV	ACGT	3 cut(s)	29, 490, 996
HpyCH4V	TGCA	2 cut(s)	647, 763
HpyF10VI	GCNNNNNNNGC	5 cut(s)	86, 294, 318, 327, 341
HpyF3I	CTNAG	7 cut(s)	81, 170, 293, 326, 583, 731, 967
HpySE526I	ACGT	3 cut(s)	29, 490, 996
Hsp92I	GRCGYC	4 cut(s)	251, 312, 344, 659
HspAI	GCGC	2 cut(s)	251, 312
KasI	GGCGCC	2 cut(s)	250, 311
KroI	GCCGGC	1 cut(s)	321
KroNI	GCCGGC	1 cut(s)	323
Ksp22I	TGATCA	1 cut(s)	606
Kzo9I	GATC	5 cut(s)	282, 448, 556, 606, 831
LmnI	GCTCC	2 cut(s)	126, 166
Lsp1109I	GCAGC	2 cut(s)	104, 368
LweI	GCATC	3 cut(s)	297, 928, 964
MaeII	ACGT	3 cut(s)	29, 490, 996
MaeIII	GTNAC	2 cut(s)	901, 985
MalI	GATC	5 cut(s)	284, 450, 558, 608, 833
MboI	GATC	5 cut(s)	282, 448, 556, 606, 831
MboII	GAAGA	2 cut(s)	553, 866
MflI	RGATCY	1 cut(s)	831
MhlI	GDGCHC	1 cut(s)	394
MlsI	TGGCCA	1 cut(s)	179
MluCI	AATT	6 cut(s)	22, 150, 268, 531, 627, 671
MluNI	TGGCCA	1 cut(s)	179
Mly113I	GGCGCC	2 cut(s)	251, 312
MlyI	GAGTC	2 cut(s)	221, 644
MmeI	TCCRAC	3 cut(s)	33, 99, 249
Mox20I	TGGCCA	1 cut(s)	179
MroNI	GCCGGC	1 cut(s)	321
MscI	TGGCCA	1 cut(s)	179
MseI	TTAA	3 cut(s)	456, 828, 1030
MslI	CAYNNNNRTG	1 cut(s)	774
Msp20I	TGGCCA	1 cut(s)	179
MspI	CCGG	5 cut(s)	274, 303, 318, 322, 474
MspR9I	CCNGG	1 cut(s)	303
MvnI	CGCG	1 cut(s)	424
MwoI	GCNNNNNNNGC	5 cut(s)	86, 294, 318, 327, 341
NaeI	GCCGGC	1 cut(s)	323
NarI	GGCGCC	2 cut(s)	251, 312
NciI	CCSGG	1 cut(s)	303
NdeI	CATATG	1 cut(s)	364
NdeII	GATC	5 cut(s)	282, 448, 556, 606, 831
NgoMIV	GCCGGC	1 cut(s)	321
NlaIV	GGNNCC	3 cut(s)	252, 313, 700
NmuCI	GTSAC	2 cut(s)	901, 985
PcsI	WCGNNNNNNNCGW	2 cut(s)	222, 459
PdiI	GCCGGC	1 cut(s)	323
PfeI	GAWTC	5 cut(s)	56, 139, 380, 811, 958
PkrI	GCNGC	5 cut(s)	91, 94, 250, 358, 423
PleI	GAGTC	2 cut(s)	221, 643
PluTI	GGCGCC	2 cut(s)	254, 315
PpsI	GAGTC	2 cut(s)	221, 643
PspFI	CCCAGC	2 cut(s)	386, 990
PspN4I	GGNNCC	3 cut(s)	252, 313, 700
PspPI	GGNCC	1 cut(s)	276
PsuI	RGATCY	1 cut(s)	831
RsaI	GTAC	3 cut(s)	500, 745, 847
RsaNI	GTAC	3 cut(s)	499, 744, 846
RseI	CAYNNNNRTG	1 cut(s)	774
SaqAI	TTAA	3 cut(s)	456, 828, 1030
SatI	GCNGC	5 cut(s)	90, 93, 249, 357, 422
Sau3AI	GATC	5 cut(s)	282, 448, 556, 606, 831
Sau96I	GGNCC	1 cut(s)	276
SchI	GAGTC	2 cut(s)	221, 644
ScrFI	CCNGG	1 cut(s)	303
SduI	GDGCHC	1 cut(s)	394
SfaNI	GCATC	3 cut(s)	297, 928, 964
SfoI	GGCGCC	2 cut(s)	252, 313
SmiMI	CAYNNNNRTG	1 cut(s)	774
SmlI	CTYRAG	1 cut(s)	632
SmoI	CTYRAG	1 cut(s)	632
Sse9I	AATT	6 cut(s)	22, 150, 268, 531, 627, 671
SsiI	CCGC	8 cut(s)	60, 87, 90, 114, 248, 422, 906, 962
SspDI	GGCGCC	2 cut(s)	250, 311
SspI	AATATT	1 cut(s)	522
StyD4I	CCNGG	1 cut(s)	301
StyI	CCWWGG	1 cut(s)	162
TaaI	ACNGT	1 cut(s)	703
TaiI	ACGT	3 cut(s)	32, 493, 999
TaqI	TCGA	4 cut(s)	236, 378, 447, 801
TasI	AATT	6 cut(s)	22, 150, 268, 531, 627, 671
TatI	WGTACW	3 cut(s)	498, 743, 845
TauI	GCSGC	3 cut(s)	92, 251, 424
TfiI	GAWTC	5 cut(s)	56, 139, 380, 811, 958
Tru1I	TTAA	3 cut(s)	456, 828, 1030
Tru9I	TTAA	3 cut(s)	456, 828, 1030
TscAI	CASTG	1 cut(s)	649
TseFI	GTSAC	2 cut(s)	901, 985
TseI	GCWGC	2 cut(s)	92, 356
Tsp45I	GTSAC	2 cut(s)	901, 985
TspDTI	ATGAA	2 cut(s)	351, 867
TspRI	CASTG	1 cut(s)	649
XagI	CCTNNNNNAGG	1 cut(s)	161
XapI	RAATTY	1 cut(s)	627
XmiI	GTMKAC	1 cut(s)	191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.