RchiOBHm_Chr5g0030241

Gamma-glutamyl

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004728
Physical Location & Seq
Forward (+)
23982938 .. 23993863
10926 bp
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UTR
Exon/CDS
Intron
PRQ30957

Sequence Viewer

Length: 633 bp
ATGATTTTAAAGAAGAATGATGCTGGTGACCATTTTCCATTATATGCCATCTGCTTGGGTTTCGAACTTCTAACAATGATCATCAGCAAGAACAAGAAAATTCTGGAGTCATTCAGTGCTGCAGATATGGCTTCAACTTTACAATTTACCGAGACAAATATTGAGGGAACTGTGTTTCAAAGATTTCCGCCTGATTTGCTTAGAAAGTTGAGTACAGATTGTATTGTCATGCAAAACCACCATTATGGTATCTCACCAGAGACGTTGGAAGAGAGCCAAGATCTATCAAGTTTTTTTAAGATCTTGACAACCAGTACTGATAAAGATGATAAGGTCTATGTCTCCACAGTACATGCATACCACTATCCTGTGACTGCCTCCCAATGGCATCTGGAGAAAAATGCTTTTGAATGGGGGTTACCGATGATTCCACACTCAGAAGATGCCATTCACGTGACTCAGCACATCGCAAACTTTTTTATCAGCGAGGCAAGGAGGTCATTAAACAGGCCACCTATTCAGGATGTCCTCGACAATCTCATCTACAATTACAGTCCAACATTTTGTGGGAAGGCAGGGAAGGGATACGATGAAGTTTACATCTTTAAACAACCTTCACTCGCACATTTGTAA

Protein Analysis

210

Amino Acids

23.96

Weight (kDa)

5.76

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 10 - 136 9.2e-07 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 188
AcsI RAATTY 1 cut(s) 99
AcvI CACGTG 1 cut(s) 454
AfaI GTAC 3 cut(s) 214, 316, 351
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 3 cut(s) 135, 179, 410
Alw26I GTCTC 3 cut(s) 146, 254, 346
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 38, 246
AsuII TTCGAA 1 cut(s) 63
BbrPI CACGTG 1 cut(s) 454
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 56
BciVI GTATCC 1 cut(s) 578
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 3 cut(s) 146, 254, 346
BfmI CTRYAG 1 cut(s) 120
BfuI GTATCC 1 cut(s) 578
BglII AGATCT 2 cut(s) 280, 300
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmcAI AGTACT 1 cut(s) 316
BmsI GCATC 3 cut(s) 10, 397, 433
BpmI CTGGAG 2 cut(s) 125, 413
Bpu14I TTCGAA 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 454
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMII CTCAG 2 cut(s) 450, 473
BseNI ACTGG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 1 cut(s) 511
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 3 cut(s) 146, 254, 346
BsmBI CGTCTC 1 cut(s) 254
BsnI GGCC 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 63
Bsp143I GATC 3 cut(s) 78, 280, 300
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 2 cut(s) 449, 472
BspMAI CTGCAG 1 cut(s) 124
BspT104I TTCGAA 1 cut(s) 63
BsrI ACTGG 1 cut(s) 312
BssMI GATC 3 cut(s) 78, 280, 300
Bst4CI ACNGT 3 cut(s) 172, 349, 554
Bst6I CTCTTC 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 454
BstBI TTCGAA 1 cut(s) 63
BstDEI CTNAG 3 cut(s) 200, 436, 459
BstEII GGTNACC 2 cut(s) 26, 417
BstF5I GGATG 1 cut(s) 529
BstKTI GATC 3 cut(s) 81, 283, 303
BstMAI GTCTC 3 cut(s) 146, 254, 346
BstMBI GATC 3 cut(s) 78, 280, 300
BstMWI GCNNNNNNNGC 2 cut(s) 128, 196
BstNSI RCATGY 1 cut(s) 356
BstPI GGTNACC 2 cut(s) 26, 417
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 280, 300
BstXI CCANNNNNNTGG 2 cut(s) 55, 245
BstYI RGATCY 2 cut(s) 280, 300
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 1 cut(s) 511
BtgZI GCGATG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 121
Csp6I GTAC 3 cut(s) 213, 315, 350
CviAII CATG 2 cut(s) 229, 353
CviJI RGCY 3 cut(s) 131, 276, 511
CviKI_1 RGCY 3 cut(s) 131, 276, 511
CviQI GTAC 3 cut(s) 213, 315, 350
DdeI CTNAG 3 cut(s) 200, 436, 459
DpnI GATC 3 cut(s) 80, 282, 302
DpnII GATC 3 cut(s) 78, 280, 300
DraI TTTAAA 2 cut(s) 9, 607
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 1 cut(s) 177
Eco72I CACGTG 1 cut(s) 454
Eco91I GGTNACC 2 cut(s) 26, 417
EcoO65I GGTNACC 2 cut(s) 26, 417
EcoT22I ATGCAT 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 2 cut(s) 232, 356
FaiI YATR 8 cut(s) 43, 45, 128, 230, 246, 339, 354, 358
FatI CATG 2 cut(s) 228, 352
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 120
GsuI CTGGAG 2 cut(s) 125, 413
HaeIII GGCC 1 cut(s) 511
Hin1II CATG 2 cut(s) 232, 356
HinfI GANTC 3 cut(s) 107, 427, 457
HphI GGTGA 2 cut(s) 38, 246
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 4 cut(s) 104, 304, 392, 521
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 3 cut(s) 565, 574, 624
HpyCH4III ACNGT 3 cut(s) 172, 349, 554
HpyCH4IV ACGT 2 cut(s) 263, 453
HpyCH4V TGCA 3 cut(s) 122, 232, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 196
HpyF3I CTNAG 3 cut(s) 200, 436, 459
HpySE526I ACGT 2 cut(s) 263, 453
Hsp92II CATG 2 cut(s) 232, 356
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 3 cut(s) 78, 280, 300
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 3 cut(s) 10, 397, 433
MaeII ACGT 2 cut(s) 263, 453
MaeIII GTNAC 4 cut(s) 26, 370, 417, 454
MalI GATC 3 cut(s) 80, 282, 302
MboI GATC 3 cut(s) 78, 280, 300
MboII GAAGA 3 cut(s) 25, 281, 452
MflI RGATCY 2 cut(s) 280, 300
MluCI AATT 3 cut(s) 99, 143, 547
MlyI GAGTC 2 cut(s) 116, 451
MmeI TCCRAC 2 cut(s) 246, 581
MnlI CCTC 5 cut(s) 157, 388, 481, 489, 539
Mph1103I ATGCAT 1 cut(s) 358
MseI TTAA 4 cut(s) 8, 297, 503, 606
MslI CAYNNNNRTG 2 cut(s) 243, 452
MwoI GCNNNNNNNGC 2 cut(s) 128, 196
NdeII GATC 3 cut(s) 78, 280, 300
NlaIII CATG 2 cut(s) 232, 356
NmuCI GTSAC 3 cut(s) 26, 370, 454
NsiI ATGCAT 1 cut(s) 358
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 63
PfeI GAWTC 1 cut(s) 427
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 2 cut(s) 115, 451
PmaCI CACGTG 1 cut(s) 454
PmlI CACGTG 1 cut(s) 454
PpsI GAGTC 2 cut(s) 115, 451
Ppu21I YACGTR 1 cut(s) 454
PspCI CACGTG 1 cut(s) 454
PspEI GGTNACC 2 cut(s) 26, 417
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 280, 300
RsaI GTAC 3 cut(s) 214, 316, 351
RsaNI GTAC 3 cut(s) 213, 315, 350
RseI CAYNNNNRTG 2 cut(s) 243, 452
SaqAI TTAA 4 cut(s) 8, 297, 503, 606
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 78, 280, 300
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 116, 451
SetI ASST 6 cut(s) 266, 336, 456, 500, 517, 616
SfaNI GCATC 3 cut(s) 10, 397, 433
SfcI CTRYAG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 63
SmiMI CAYNNNNRTG 2 cut(s) 243, 452
Sse9I AATT 3 cut(s) 99, 143, 547
SsiI CCGC 1 cut(s) 188
SspI AATATT 1 cut(s) 160
TaaI ACNGT 3 cut(s) 172, 349, 554
TaiI ACGT 2 cut(s) 266, 456
TaqI TCGA 2 cut(s) 63, 531
TasI AATT 3 cut(s) 99, 143, 547
TatI WGTACW 3 cut(s) 212, 314, 349
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 4 cut(s) 8, 297, 503, 606
Tru9I TTAA 4 cut(s) 8, 297, 503, 606
TscAI CASTG 1 cut(s) 121
TseFI GTSAC 3 cut(s) 26, 370, 454
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 3 cut(s) 26, 370, 454
TspDTI ATGAA 1 cut(s) 606
TspRI CASTG 1 cut(s) 121
XapI RAATTY 1 cut(s) 99
XceI RCATGY 1 cut(s) 356
ZrmI AGTACT 1 cut(s) 316
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.