Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004275

Physical Location & Seq

Genomic Coordinates Reverse (-)

1830425 .. 1840801

Gene Structure 10377 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1005500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 642 bp

ATGGAATCAGCGGTGGGTTCGAATCAAGCGGCGGAGTTCGACTACTTGTTCAAGCTGTTGATGATCGGCGACTCAGGCGTCGGCAAGAGCAGCCTACTCCTCAGCTTCACCTCCGACTCCTTTGAAGACCTTTCGCCCACCATTGGTGTTGATTTTAAGGTCAAATATGTTACTCTCGGAGGCAAAAAACTCAAGCTTGCAATTTGGGATACAGCTGGTCAAGAGAGGTTTAGAACCTTGACGAGTTCATATTACAGAGGTGCACAAGGGATCATTATGGTTTACGATGTAACGAGGCGAGAAACATTTACAAACCTTTGTGATGTATGGGCCAAAGAAATAGAACTCTATTCAACAAATCAAGACTGCATCAAGATGCTTGTTGGGAACAAAGTTGACAAGGAAAGCGACAGGGTTGTAACAAAGAAAGAGGGAATAAATTTTGCTAGGGAATCTGGGTGCCTCTTTATTGAGTGCAGTGCTAAAACTCGAGTTAATGTGCAGCAGTGCTTCGAAGAGCTTGTTTTAAAGATTCTGGATACTCCTAGTCTCTTAGCCGAGGGTTCTAAAGGGGTGAAGAAGCATAACATCTTTAAGGAGAAACCGCCACAGTCCGATGCATCGACCAGCGGTTGTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ras

Protein Analysis

214

Amino Acids

23.65

Weight (kDa)

6.61

Isoelectric Point (pI)

29.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	17 - 175	2.1e-17	ADP-ribosylation factor family
Ras	PF00071	18 - 179	4.4e-57	Ras family
Roc	PF08477	18 - 133	2.1e-31	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43890 AT1G43890 AT1G43890
fragaria_vesca	FvH4_1g24410 FvH4_2g15140
malus_domestica	MD00G1163000.v1.1 MD01G1005500.v1.1 MD05G1010800.v1.1 MD10G1009500.v1.1
prunus_persica	Prupe.4G271600_v2.0.a1 Prupe.4G271600_v2.0.a1 Prupe.8G013500_v2.0.a1
pyrus_communis	pycom05g00580 pycom10g00570 pycom15g30450
rosa_chinensis	RchiOBHm_Chr2g0121291 RchiOBHm_Chr6g0277801
rosa_laevigata	RLG00000013268 RLG00000018616
rosa_multiflora	Rmu_sc0000079.1_g000029
rosa_roxburghii	Rroxscaffold_7G00190920
rosa_rugosa	Rorug02G0233100 Rorug02G0233200 Rorug06G0111200
rosa_samantha	Rh2AG290000 Rh2DG314800 Rh6AG222100 Rh6BG225500 Rh6CG228200 Rh6DG219200
rosa_wichuraiana	Rw2G023250 Rw6G019410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	77
AccB1I	GGYRCC	1 cut(s)	459
AciI	CCGC	5 cut(s)	11, 29, 32, 605, 630
AclWI	GGATC	1 cut(s)	278
AcsI	RAATTY	1 cut(s)	439
AcyI	GRCGYC	1 cut(s)	78
AfiI	CCNNNNNNNGG	1 cut(s)	143
AgsI	TTSAA	3 cut(s)	52, 125, 354
AluBI	AGCT	5 cut(s)	55, 105, 196, 215, 520
AluI	AGCT	5 cut(s)	55, 105, 196, 215, 520
Alw21I	GWGCWC	1 cut(s)	265
Alw26I	GTCTC	1 cut(s)	554
Alw44I	GTGCAC	1 cut(s)	261
AlwI	GGATC	1 cut(s)	278
Ama87I	CYCGRG	1 cut(s)	489
AoxI	GGCC	1 cut(s)	330
ApaLI	GTGCAC	1 cut(s)	261
ApeKI	GCWGC	2 cut(s)	90, 502
ApoI	RAATTY	1 cut(s)	439
AspS9I	GGNCC	1 cut(s)	330
AsuHPI	GGTGA	2 cut(s)	100, 586
AsuII	TTCGAA	2 cut(s)	20, 513
AvaI	CYCGRG	1 cut(s)	489
BaeGI	GKGCMC	1 cut(s)	265
BanI	GGYRCC	1 cut(s)	459
BbsI	GAAGAC	1 cut(s)	132
Bbv12I	GWGCWC	1 cut(s)	265
BbvCI	CCTCAGC	1 cut(s)	101
BbvI	GCAGC	2 cut(s)	102, 514
BciVI	GTATCC	2 cut(s)	202, 532
BcoDI	GTCTC	1 cut(s)	554
BfaI	CTAG	2 cut(s)	447, 546
BfuI	GTATCC	2 cut(s)	202, 532
BisI	GCNGC	3 cut(s)	30, 91, 503
BlsI	GCNGC	3 cut(s)	31, 92, 504
BmeT110I	CYCGRG	1 cut(s)	489
BmgT120I	GGNCC	1 cut(s)	330
BmiI	GGNNCC	1 cut(s)	461
BmsI	GCATC	4 cut(s)	366, 378, 607, 629
BpiI	GAAGAC	1 cut(s)	132
Bpu10I	CCTNAGC	1 cut(s)	101
Bpu14I	TTCGAA	2 cut(s)	20, 513
BpuEI	CTTGAG	1 cut(s)	176
BsaHI	GRCGYC	1 cut(s)	78
BsaJI	CCNNGG	1 cut(s)	558
Bsc4I	CCNNNNNNNGG	1 cut(s)	143
BseDI	CCNNGG	1 cut(s)	558
BseLI	CCNNNNNNNGG	1 cut(s)	143
BseMII	CTCAG	2 cut(s)	87, 115
BseRI	GAGGAG	1 cut(s)	89
BseSI	GKGCMC	1 cut(s)	265
BseXI	GCAGC	2 cut(s)	102, 514
BsgI	GTGCAG	2 cut(s)	496, 521
BshFI	GGCC	1 cut(s)	332
BshNI	GGYRCC	1 cut(s)	459
BsiHKAI	GWGCWC	1 cut(s)	265
BsiHKCI	CYCGRG	1 cut(s)	489
BslI	CCNNNNNNNGG	1 cut(s)	143
BsmAI	GTCTC	1 cut(s)	554
BsnI	GGCC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	489
Bsp119I	TTCGAA	2 cut(s)	20, 513
Bsp1286I	GDGCHC	1 cut(s)	265
Bsp143I	GATC	2 cut(s)	63, 270
BspACI	CCGC	5 cut(s)	11, 29, 32, 605, 630
BspANI	GGCC	1 cut(s)	332
BspCNI	CTCAG	2 cut(s)	86, 114
BspLI	GGNNCC	1 cut(s)	461
BspPI	GGATC	1 cut(s)	278
BspQI	GCTCTTC	1 cut(s)	510
BspT104I	TTCGAA	2 cut(s)	20, 513
BspT107I	GGYRCC	1 cut(s)	459
BssECI	CCNNGG	1 cut(s)	558
BssMI	GATC	2 cut(s)	63, 270
BssNI	GRCGYC	1 cut(s)	78
Bst4CI	ACNGT	1 cut(s)	612
Bst6I	CTCTTC	1 cut(s)	510
BstACI	GRCGYC	1 cut(s)	78
BstBI	TTCGAA	2 cut(s)	20, 513
BstC8I	GCNNGC	1 cut(s)	198
BstDEI	CTNAG	3 cut(s)	73, 101, 553
BstKTI	GATC	2 cut(s)	66, 273
BstMAI	GTCTC	1 cut(s)	554
BstMBI	GATC	2 cut(s)	63, 270
BstMWI	GCNNNNNNNGC	3 cut(s)	75, 90, 636
BstSLI	GKGCMC	1 cut(s)	265
BstV1I	GCAGC	2 cut(s)	102, 514
BstV2I	GAAGAC	1 cut(s)	132
BsuI	GTATCC	2 cut(s)	202, 532
BsuRI	GGCC	1 cut(s)	332
BtsI	GCAGTG	2 cut(s)	484, 512
BtsIMutI	CAGTG	2 cut(s)	484, 512
Cac8I	GCNNGC	1 cut(s)	198
Cfr13I	GGNCC	1 cut(s)	330
CseI	GACGC	1 cut(s)	67
CviJI	RGCY	8 cut(s)	55, 93, 105, 196, 215, 332, 520, 557
CviKI_1	RGCY	8 cut(s)	55, 93, 105, 196, 215, 332, 520, 557
DdeI	CTNAG	3 cut(s)	73, 101, 553
DpnI	GATC	2 cut(s)	65, 272
DpnII	GATC	2 cut(s)	63, 270
DraI	TTTAAA	1 cut(s)	528
DrdI	GACNNNNNNGTC	1 cut(s)	77
DseDI	GACNNNNNNGTC	1 cut(s)	77
Eam1104I	CTCTTC	1 cut(s)	510
EarI	CTCTTC	1 cut(s)	510
EciI	GGCGGA	1 cut(s)	47
Eco88I	CYCGRG	1 cut(s)	489
EcoT22I	ATGCAT	1 cut(s)	622
FaiI	YATR	5 cut(s)	168, 250, 278, 328, 585
Fnu4HI	GCNGC	3 cut(s)	30, 91, 503
Fsp4HI	GCNGC	3 cut(s)	30, 91, 503
FspBI	CTAG	2 cut(s)	447, 546
GluI	GCNGC	3 cut(s)	30, 91, 503
HaeIII	GGCC	1 cut(s)	332
HgaI	GACGC	1 cut(s)	67
Hin1I	GRCGYC	1 cut(s)	78
HincII	GTYRAC	1 cut(s)	397
HindII	GTYRAC	1 cut(s)	397
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	6 cut(s)	5, 22, 71, 116, 452, 532
HphI	GGTGA	2 cut(s)	100, 586
Hpy166II	GTNNAC	3 cut(s)	263, 283, 397
Hpy188I	TCNGA	3 cut(s)	115, 179, 616
Hpy188III	TCNNGA	4 cut(s)	221, 362, 373, 536
Hpy8I	GTNNAC	3 cut(s)	263, 283, 397
Hpy99I	CGWCG	1 cut(s)	83
HpyCH4III	ACNGT	1 cut(s)	612
HpyCH4V	TGCA	6 cut(s)	200, 263, 369, 477, 502, 620
HpyF10VI	GCNNNNNNNGC	3 cut(s)	75, 90, 636
HpyF3I	CTNAG	3 cut(s)	73, 101, 553
Hsp92I	GRCGYC	1 cut(s)	78
Kzo9I	GATC	2 cut(s)	63, 270
LguI	GCTCTTC	1 cut(s)	510
LpnPI	CCDG	5 cut(s)	60, 201, 397, 441, 521
Lsp1109I	GCAGC	2 cut(s)	102, 514
LweI	GCATC	4 cut(s)	366, 378, 607, 629
MaeI	CTAG	2 cut(s)	447, 546
MaeIII	GTNAC	3 cut(s)	169, 289, 418
MalI	GATC	2 cut(s)	65, 272
MboI	GATC	2 cut(s)	63, 270
MboII	GAAGA	3 cut(s)	137, 527, 589
MhlI	GDGCHC	1 cut(s)	265
MluCI	AATT	2 cut(s)	201, 439
MlyI	GAGTC	2 cut(s)	65, 110
MmeI	TCCRAC	1 cut(s)	138
MnlI	CCTC	9 cut(s)	110, 121, 173, 219, 251, 288, 424, 473, 553
Mph1103I	ATGCAT	1 cut(s)	622
MseI	TTAA	4 cut(s)	156, 495, 527, 594
MslI	CAYNNNNRTG	1 cut(s)	374
MspA1I	CMGCKG	3 cut(s)	11, 215, 630
MwoI	GCNNNNNNNGC	3 cut(s)	75, 90, 636
NdeII	GATC	2 cut(s)	63, 270
NlaIV	GGNNCC	1 cut(s)	461
NmeAIII	GCCGAG	1 cut(s)	583
NsiI	ATGCAT	1 cut(s)	622
NspV	TTCGAA	2 cut(s)	20, 513
PaeR7I	CTCGAG	1 cut(s)	489
PciSI	GCTCTTC	1 cut(s)	510
PfeI	GAWTC	4 cut(s)	5, 22, 452, 532
PkrI	GCNGC	3 cut(s)	31, 92, 504
PleI	GAGTC	2 cut(s)	65, 110
PpsI	GAGTC	2 cut(s)	65, 110
PspN4I	GGNNCC	1 cut(s)	461
PspPI	GGNCC	1 cut(s)	330
PspXI	VCTCGAGB	1 cut(s)	489
PvuII	CAGCTG	1 cut(s)	215
RseI	CAYNNNNRTG	1 cut(s)	374
SapI	GCTCTTC	1 cut(s)	510
SaqAI	TTAA	4 cut(s)	156, 495, 527, 594
SatI	GCNGC	3 cut(s)	30, 91, 503
Sau3AI	GATC	2 cut(s)	63, 270
Sau96I	GGNCC	1 cut(s)	330
SchI	GAGTC	2 cut(s)	65, 110
SduI	GDGCHC	1 cut(s)	265
SfaNI	GCATC	4 cut(s)	366, 378, 607, 629
Sfr274I	CTCGAG	1 cut(s)	489
SfuI	TTCGAA	2 cut(s)	20, 513
SlaI	CTCGAG	1 cut(s)	489
SmiMI	CAYNNNNRTG	1 cut(s)	374
SmlI	CTYRAG	2 cut(s)	191, 489
SmoI	CTYRAG	2 cut(s)	191, 489
Sse9I	AATT	2 cut(s)	201, 439
SsiI	CCGC	5 cut(s)	11, 29, 32, 605, 630
SspMI	CTAG	2 cut(s)	447, 546
TaaI	ACNGT	1 cut(s)	612
TaqI	TCGA	5 cut(s)	20, 39, 490, 513, 623
TasI	AATT	2 cut(s)	201, 439
TauI	GCSGC	1 cut(s)	32
TfiI	GAWTC	4 cut(s)	5, 22, 452, 532
Tru1I	TTAA	4 cut(s)	156, 495, 527, 594
Tru9I	TTAA	4 cut(s)	156, 495, 527, 594
TscAI	CASTG	2 cut(s)	484, 512
TseI	GCWGC	2 cut(s)	90, 502
TspDTI	ATGAA	1 cut(s)	237
TspRI	CASTG	2 cut(s)	484, 512
VneI	GTGCAC	1 cut(s)	261
XapI	RAATTY	1 cut(s)	439
XhoI	CTCGAG	1 cut(s)	489
XspI	CTAG	2 cut(s)	447, 546
Zsp2I	ATGCAT	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.