Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004275

Physical Location & Seq

Genomic Coordinates Reverse (-)

1332429 .. 1335325

Gene Structure 2897 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1009500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 636 bp

ATGGAGTCCGCAACGACGTCGTCTGGGGAGTCGGAGTTCGATTACTTGTTCAAGCTGTTGCTGATTGGGGACTCGGGGGTCGGGAAGAGCACGCTGCTCTTGAGTTTTACTTCCGATACCTTTGAGGATCTATCTCCTACTATCGGTGTGGATTTTAAGATAAAGCATGTCACTCTTGGGGGTAAAAAATTAAAGCTTGCAATTTGGGACACAGCTGGACAGGAAAGATTTAGAACTCTTACTAGTTCATATTACAGAGGAGCTCAAGGGATAATAATGGTGTATGATGTAACACGAAGAGAAACGTTTACGAATTTGTCTGATATATGGGCTAAAGAAATCGATCTGTACTCCACAAGTCAAGATTGCATCAAGATGCTTGTCGGAAACAAAGTCGATAAGGAAAGTGAAAGGACTGTAAGTAAAAAAGAGGGGATGGAATTTGCGAGGGAATATGGATGCCTTTTTCTTGAGTGCAGTGCCAAAACACGAGTAAATGTAGAGCAGTGCTTTGAGGAGCTTGTCTTAAAGATATTGGAAACACCAAGTCTCGTAGCTGAGGGCTCAGCAGGTGTGAAAAAGAACATCTTCAAGCAAAAACCTCCAGAATCTGATGCATCAAGTGGCTGCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ras

Protein Analysis

212

Amino Acids

23.44

Weight (kDa)

5.27

Isoelectric Point (pI)

29.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	17 - 175	3.7e-18	ADP-ribosylation factor family
Ras	PF00071	18 - 179	1.2e-58	Ras family
Roc	PF08477	18 - 133	3.2e-31	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43890 AT1G43890 AT1G43890
fragaria_vesca	FvH4_1g24410 FvH4_2g15140
malus_domestica	MD00G1163000.v1.1 MD01G1005500.v1.1 MD05G1010800.v1.1 MD10G1009500.v1.1
prunus_persica	Prupe.4G271600_v2.0.a1 Prupe.4G271600_v2.0.a1 Prupe.8G013500_v2.0.a1
pyrus_communis	pycom05g00580 pycom10g00570 pycom15g30450
rosa_chinensis	RchiOBHm_Chr2g0121291 RchiOBHm_Chr6g0277801
rosa_laevigata	RLG00000013268 RLG00000018616
rosa_multiflora	Rmu_sc0000079.1_g000029
rosa_roxburghii	Rroxscaffold_7G00190920
rosa_rugosa	Rorug02G0233100 Rorug02G0233200 Rorug06G0111200
rosa_samantha	Rh2AG290000 Rh2DG314800 Rh6AG222100 Rh6BG225500 Rh6CG228200 Rh6DG219200
rosa_wichuraiana	Rw2G023250 Rw6G019410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	560
AasI	GACNNNNNNGTC	1 cut(s)	77
AatII	GACGTC	1 cut(s)	20
Acc36I	ACCTGC	1 cut(s)	560
AciI	CCGC	1 cut(s)	9
AclI	AACGTT	1 cut(s)	305
AclWI	GGATC	1 cut(s)	135
AcsI	RAATTY	2 cut(s)	313, 440
AcyI	GRCGYC	1 cut(s)	17
AfaI	GTAC	1 cut(s)	350
AfiI	CCNNNNNNNGG	1 cut(s)	143
AgsI	TTSAA	2 cut(s)	52, 592
AhlI	ACTAGT	1 cut(s)	242
AloI	GAACNNNNNNTCC	2 cut(s)	20, 52
AluBI	AGCT	6 cut(s)	55, 196, 215, 263, 520, 557
AluI	AGCT	6 cut(s)	55, 196, 215, 263, 520, 557
Alw21I	GWGCWC	2 cut(s)	92, 265
Alw26I	GTCTC	1 cut(s)	554
AlwI	GGATC	1 cut(s)	135
AlwNI	CAGNNNCTG	1 cut(s)	611
Ama87I	CYCGRG	1 cut(s)	73
ApeKI	GCWGC	3 cut(s)	94, 627, 630
ApoI	RAATTY	2 cut(s)	313, 440
Asp700I	GAANNNNTTC	1 cut(s)	587
AvaI	CYCGRG	1 cut(s)	73
BanII	GRGCYC	2 cut(s)	265, 566
BauI	CACGAG	1 cut(s)	489
Bbv12I	GWGCWC	2 cut(s)	92, 265
BbvCI	CCTCAGC	1 cut(s)	558
BbvI	GCAGC	3 cut(s)	81, 614, 617
BccI	CCATC	1 cut(s)	430
BcgI	CGANNNNNNTGC	1 cut(s)	34
BcoDI	GTCTC	1 cut(s)	554
BcuI	ACTAGT	1 cut(s)	242
BfaI	CTAG	1 cut(s)	243
BfuAI	ACCTGC	1 cut(s)	560
BisI	GCNGC	3 cut(s)	95, 628, 631
BlpI	GCTNAGC	1 cut(s)	565
BlsI	GCNGC	3 cut(s)	96, 629, 632
BmeT110I	CYCGRG	1 cut(s)	73
BmsI	GCATC	5 cut(s)	366, 378, 449, 604, 626
BpmI	CTGGAG	1 cut(s)	588
Bpu10I	CCTNAGC	1 cut(s)	558
Bpu1102I	GCTNAGC	1 cut(s)	565
BpuEI	CTTGAG	3 cut(s)	121, 249, 491
Bsa29I	ATCGAT	1 cut(s)	342
BsaHI	GRCGYC	1 cut(s)	17
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bsc4I	CCNNNNNNNGG	1 cut(s)	143
BseCI	ATCGAT	1 cut(s)	342
BseGI	GGATG	2 cut(s)	441, 464
BseLI	CCNNNNNNNGG	1 cut(s)	143
BseMII	CTCAG	2 cut(s)	549, 579
BseRI	GAGGAG	2 cut(s)	273, 530
BseXI	GCAGC	3 cut(s)	81, 614, 617
BsgI	GTGCAG	1 cut(s)	496
BshVI	ATCGAT	1 cut(s)	342
BsiHKAI	GWGCWC	2 cut(s)	92, 265
BsiHKCI	CYCGRG	1 cut(s)	73
BslFI	GGGAC	2 cut(s)	83, 221
BslI	CCNNNNNNNGG	1 cut(s)	143
BsmAI	GTCTC	1 cut(s)	554
BsmFI	GGGAC	2 cut(s)	83, 221
BsoBI	CYCGRG	1 cut(s)	73
Bsp1286I	GDGCHC	3 cut(s)	92, 265, 566
Bsp143I	GATC	2 cut(s)	127, 343
Bsp1720I	GCTNAGC	1 cut(s)	565
BspACI	CCGC	1 cut(s)	9
BspCNI	CTCAG	2 cut(s)	550, 578
BspDI	ATCGAT	1 cut(s)	342
BspMI	ACCTGC	1 cut(s)	560
BspPI	GGATC	1 cut(s)	135
BspQI	GCTCTTC	1 cut(s)	80
BssMI	GATC	2 cut(s)	127, 343
BssNI	GRCGYC	1 cut(s)	17
BssSI	CACGAG	1 cut(s)	489
Bst2BI	CACGAG	1 cut(s)	489
Bst4CI	ACNGT	1 cut(s)	418
Bst6I	CTCTTC	2 cut(s)	80, 292
BstACI	GRCGYC	1 cut(s)	17
BstC8I	GCNNGC	2 cut(s)	92, 198
BstDEI	CTNAG	2 cut(s)	558, 565
BstF5I	GGATG	2 cut(s)	441, 464
BstKTI	GATC	2 cut(s)	130, 346
BstMAI	GTCTC	1 cut(s)	554
BstMBI	GATC	2 cut(s)	127, 343
BstNSI	RCATGY	1 cut(s)	170
BstV1I	GCAGC	3 cut(s)	81, 614, 617
BstX2I	RGATCY	1 cut(s)	127
BstYI	RGATCY	1 cut(s)	127
Bsu15I	ATCGAT	1 cut(s)	342
BsuTUI	ATCGAT	1 cut(s)	342
BtsCI	GGATG	2 cut(s)	441, 464
BtsI	GCAGTG	2 cut(s)	484, 512
BtsIMutI	CAGTG	2 cut(s)	484, 512
BveI	ACCTGC	1 cut(s)	560
Cac8I	GCNNGC	2 cut(s)	92, 198
CaiI	CAGNNNCTG	1 cut(s)	611
ClaI	ATCGAT	1 cut(s)	342
Csp6I	GTAC	1 cut(s)	349
CviAII	CATG	1 cut(s)	167
CviJI	RGCY	9 cut(s)	55, 196, 215, 263, 332, 520, 557, 564, 627
CviKI_1	RGCY	9 cut(s)	55, 196, 215, 263, 332, 520, 557, 564, 627
CviQI	GTAC	1 cut(s)	349
DdeI	CTNAG	2 cut(s)	558, 565
DpnI	GATC	2 cut(s)	129, 345
DpnII	GATC	2 cut(s)	127, 343
DrdI	GACNNNNNNGTC	1 cut(s)	77
DseDI	GACNNNNNNGTC	1 cut(s)	77
Eam1104I	CTCTTC	2 cut(s)	80, 292
EarI	CTCTTC	2 cut(s)	80, 292
Ecl136II	GAGCTC	1 cut(s)	263
Eco24I	GRGCYC	2 cut(s)	265, 566
Eco53kI	GAGCTC	1 cut(s)	263
Eco88I	CYCGRG	1 cut(s)	73
EcoICRI	GAGCTC	1 cut(s)	263
EcoT22I	ATGCAT	1 cut(s)	619
EcoT38I	GRGCYC	2 cut(s)	265, 566
FaeI	CATG	1 cut(s)	170
FaiI	YATR	6 cut(s)	168, 250, 285, 326, 328, 456
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	2 cut(s)	83, 221
FatI	CATG	1 cut(s)	166
Fnu4HI	GCNGC	3 cut(s)	95, 628, 631
FokI	GGATG	2 cut(s)	448, 471
FriOI	GRGCYC	2 cut(s)	265, 566
Fsp4HI	GCNGC	3 cut(s)	95, 628, 631
FspBI	CTAG	1 cut(s)	243
GluI	GCNGC	3 cut(s)	95, 628, 631
GsuI	CTGGAG	1 cut(s)	588
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	1 cut(s)	170
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	4 cut(s)	5, 29, 71, 608
Hpy166II	GTNNAC	1 cut(s)	309
Hpy188I	TCNGA	5 cut(s)	34, 115, 322, 386, 613
Hpy188III	TCNNGA	6 cut(s)	82, 100, 362, 373, 470, 605
Hpy8I	GTNNAC	1 cut(s)	309
Hpy99I	CGWCG	2 cut(s)	19, 22
HpyCH4III	ACNGT	1 cut(s)	418
HpyCH4IV	ACGT	2 cut(s)	17, 305
HpyCH4V	TGCA	4 cut(s)	200, 369, 477, 617
HpyF3I	CTNAG	2 cut(s)	558, 565
HpySE526I	ACGT	2 cut(s)	17, 305
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	1 cut(s)	170
Kzo9I	GATC	2 cut(s)	127, 343
LguI	GCTCTTC	1 cut(s)	80
LmnI	GCTCC	2 cut(s)	260, 517
LpnPI	CCDG	5 cut(s)	9, 201, 206, 555, 618
Lsp1109I	GCAGC	3 cut(s)	81, 614, 617
LweI	GCATC	5 cut(s)	366, 378, 449, 604, 626
MaeI	CTAG	1 cut(s)	243
MaeII	ACGT	2 cut(s)	17, 305
MaeIII	GTNAC	2 cut(s)	169, 289
MalI	GATC	2 cut(s)	129, 345
MboI	GATC	2 cut(s)	127, 343
MboII	GAAGA	3 cut(s)	97, 309, 580
MflI	RGATCY	1 cut(s)	127
MhlI	GDGCHC	3 cut(s)	92, 265, 566
MluCI	AATT	4 cut(s)	188, 201, 313, 440
MlyI	GAGTC	3 cut(s)	14, 38, 65
MmeI	TCCRAC	2 cut(s)	12, 364
MnlI	CCTC	7 cut(s)	118, 251, 424, 441, 508, 553, 612
Mph1103I	ATGCAT	1 cut(s)	619
MroXI	GAANNNNTTC	1 cut(s)	587
MseI	TTAA	3 cut(s)	156, 191, 527
MslI	CAYNNNNRTG	1 cut(s)	374
MspA1I	CMGCKG	1 cut(s)	215
NdeII	GATC	2 cut(s)	127, 343
NlaIII	CATG	1 cut(s)	170
NmuCI	GTSAC	1 cut(s)	169
NsiI	ATGCAT	1 cut(s)	619
NspI	RCATGY	1 cut(s)	170
PaqCI	CACCTGC	1 cut(s)	560
PciSI	GCTCTTC	1 cut(s)	80
PdmI	GAANNNNTTC	1 cut(s)	587
PfeI	GAWTC	1 cut(s)	608
PflFI	GACNNNGTC	1 cut(s)	19
PkrI	GCNGC	3 cut(s)	96, 629, 632
PleI	GAGTC	3 cut(s)	13, 37, 65
PpsI	GAGTC	3 cut(s)	13, 37, 65
Psp124BI	GAGCTC	1 cut(s)	265
Psp1406I	AACGTT	1 cut(s)	305
PstNI	CAGNNNCTG	1 cut(s)	611
PsuI	RGATCY	1 cut(s)	127
PsyI	GACNNNGTC	1 cut(s)	19
PvuII	CAGCTG	1 cut(s)	215
RsaI	GTAC	1 cut(s)	350
RsaNI	GTAC	1 cut(s)	349
RseI	CAYNNNNRTG	1 cut(s)	374
SacI	GAGCTC	1 cut(s)	265
SapI	GCTCTTC	1 cut(s)	80
SaqAI	TTAA	3 cut(s)	156, 191, 527
SatI	GCNGC	3 cut(s)	95, 628, 631
Sau3AI	GATC	2 cut(s)	127, 343
SchI	GAGTC	3 cut(s)	14, 38, 65
SduI	GDGCHC	3 cut(s)	92, 265, 566
SfaNI	GCATC	5 cut(s)	366, 378, 449, 604, 626
SmiMI	CAYNNNNRTG	1 cut(s)	374
SmlI	CTYRAG	3 cut(s)	100, 264, 470
SmoI	CTYRAG	3 cut(s)	100, 264, 470
SpeI	ACTAGT	1 cut(s)	242
Sse9I	AATT	4 cut(s)	188, 201, 313, 440
SsiI	CCGC	1 cut(s)	9
SspMI	CTAG	1 cut(s)	243
SstI	GAGCTC	1 cut(s)	265
TaaI	ACNGT	1 cut(s)	418
TaiI	ACGT	2 cut(s)	20, 308
TaqI	TCGA	3 cut(s)	39, 342, 396
TasI	AATT	4 cut(s)	188, 201, 313, 440
TatI	WGTACW	1 cut(s)	348
TfiI	GAWTC	1 cut(s)	608
Tru1I	TTAA	3 cut(s)	156, 191, 527
Tru9I	TTAA	3 cut(s)	156, 191, 527
TscAI	CASTG	2 cut(s)	484, 512
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	3 cut(s)	94, 627, 630
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	1 cut(s)	237
TspRI	CASTG	2 cut(s)	484, 512
Tth111I	GACNNNGTC	1 cut(s)	19
XapI	RAATTY	2 cut(s)	313, 440
XceI	RCATGY	1 cut(s)	170
XmnI	GAANNNNTTC	1 cut(s)	587
XspI	CTAG	1 cut(s)	243
ZraI	GACGTC	1 cut(s)	18
Zsp2I	ATGCAT	1 cut(s)	619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.