Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004275

Physical Location & Seq

Genomic Coordinates Reverse (-)

2145693 .. 2148127

Gene Structure 2435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1010800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 639 bp

ATGGAATCTGCAACGACGTCGTCTGGTGAGTCGGAGTTCGATTACTTGTTCAAGCTGCTGCTGATTGGGGACTCGGGGGTTGGGAAGAGCACGCTCCTCTTGAGTTTCACTTCCGACACCTTCGAGGATCTCTCTCCAACTATCGGTGTTGATTTTAAGATAAAGCATGTCACTCTTGGGGGTAAGAAATTGAAGCTTGCAATTTGGGACACAGCTGGACAGGAAAGATTTAGAACTCTTACCAGTTCATATTACAGAGGAGCTCAAGGGATAATAATGGTGTATGATGTGACACGAAGAGAAACATTTACGAATTTGTCTGATATATGGGCGAAAGAGATTGATCTGTATTCCACTAGTCAAGATTGCATCAAGATGCTTGTTGGAAACAAAGTCGATAAGGAAAGCGAAAGGACGGTAAGTAAAAAAGAGGGGATGGAATTTGCTAGGGAATATGGATGCCTTTTTCTTGAGTGCAGTGCAAAAACTCGAGTAAATGTAGAGCAGTGCTTTGAGGAGCTTGTCTTAAAGATATTGGAAACACCGAGTCTCGTGGCTGAGGGATCAGCAGGTGTGAAAAAGAACATCTTCAAGCAAAAAGCTCCTCAATCTGATGAATCAGTAAGTGGCTGTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ras

Protein Analysis

213

Amino Acids

23.57

Weight (kDa)

5.27

Isoelectric Point (pI)

30.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	17 - 175	3.7e-18	ADP-ribosylation factor family
Ras	PF00071	18 - 179	1.2e-58	Ras family
Roc	PF08477	18 - 133	3.3e-31	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43890 AT1G43890 AT1G43890
fragaria_vesca	FvH4_1g24410 FvH4_2g15140
malus_domestica	MD00G1163000.v1.1 MD01G1005500.v1.1 MD05G1010800.v1.1 MD10G1009500.v1.1
prunus_persica	Prupe.4G271600_v2.0.a1 Prupe.4G271600_v2.0.a1 Prupe.8G013500_v2.0.a1
pyrus_communis	pycom05g00580 pycom10g00570 pycom15g30450
rosa_chinensis	RchiOBHm_Chr2g0121291 RchiOBHm_Chr6g0277801
rosa_laevigata	RLG00000013268 RLG00000018616
rosa_multiflora	Rmu_sc0000079.1_g000029
rosa_roxburghii	Rroxscaffold_7G00190920
rosa_rugosa	Rorug02G0233100 Rorug02G0233200 Rorug06G0111200
rosa_samantha	Rh2AG290000 Rh2DG314800 Rh6AG222100 Rh6BG225500 Rh6CG228200 Rh6DG219200
rosa_wichuraiana	Rw2G023250 Rw6G019410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	560
AatII	GACGTC	1 cut(s)	20
Acc36I	ACCTGC	1 cut(s)	560
AclWI	GGATC	2 cut(s)	135, 571
AcsI	RAATTY	2 cut(s)	313, 440
AcyI	GRCGYC	1 cut(s)	17
AfiI	CCNNNNNNNGG	1 cut(s)	143
AgsI	TTSAA	3 cut(s)	52, 193, 592
AhlI	ACTAGT	1 cut(s)	356
AluBI	AGCT	6 cut(s)	55, 196, 215, 263, 520, 602
AluI	AGCT	6 cut(s)	55, 196, 215, 263, 520, 602
Alw21I	GWGCWC	2 cut(s)	92, 265
Alw26I	GTCTC	1 cut(s)	554
AlwI	GGATC	2 cut(s)	135, 571
Ama87I	CYCGRG	2 cut(s)	73, 489
ApeKI	GCWGC	2 cut(s)	55, 58
ApoI	RAATTY	2 cut(s)	313, 440
Asp700I	GAANNNNTTC	1 cut(s)	587
AsuHPI	GGTGA	1 cut(s)	38
AvaI	CYCGRG	2 cut(s)	73, 489
BanII	GRGCYC	1 cut(s)	265
BauI	CACGAG	1 cut(s)	551
Bbv12I	GWGCWC	2 cut(s)	92, 265
BbvCI	CCTCAGC	1 cut(s)	558
BbvI	GCAGC	2 cut(s)	42, 45
BccI	CCATC	1 cut(s)	430
BcgI	CGANNNNNNTGC	1 cut(s)	34
BcoDI	GTCTC	1 cut(s)	554
BcuI	ACTAGT	1 cut(s)	356
BfaI	CTAG	2 cut(s)	357, 447
BfuAI	ACCTGC	1 cut(s)	560
BisI	GCNGC	2 cut(s)	56, 59
BlsI	GCNGC	2 cut(s)	57, 60
BmeT110I	CYCGRG	2 cut(s)	73, 489
BmsI	GCATC	3 cut(s)	366, 378, 449
BplI	GAGNNNNNCTC	2 cut(s)	116, 148
Bpu10I	CCTNAGC	1 cut(s)	558
BpuEI	CTTGAG	3 cut(s)	121, 249, 491
BsaHI	GRCGYC	1 cut(s)	17
Bsc4I	CCNNNNNNNGG	1 cut(s)	143
Bse1I	ACTGG	1 cut(s)	243
BseGI	GGATG	2 cut(s)	441, 464
BseLI	CCNNNNNNNGG	1 cut(s)	143
BseMII	CTCAG	1 cut(s)	549
BseNI	ACTGG	1 cut(s)	243
BseRI	GAGGAG	4 cut(s)	86, 273, 530, 594
BseXI	GCAGC	2 cut(s)	42, 45
BsgI	GTGCAG	1 cut(s)	496
BsiHKAI	GWGCWC	2 cut(s)	92, 265
BsiHKCI	CYCGRG	2 cut(s)	73, 489
BslFI	GGGAC	2 cut(s)	83, 221
BslI	CCNNNNNNNGG	1 cut(s)	143
BsmAI	GTCTC	1 cut(s)	554
BsmFI	GGGAC	2 cut(s)	83, 221
BsoBI	CYCGRG	2 cut(s)	73, 489
Bsp1286I	GDGCHC	2 cut(s)	92, 265
Bsp143I	GATC	3 cut(s)	127, 343, 563
BspCNI	CTCAG	1 cut(s)	550
BspMI	ACCTGC	1 cut(s)	560
BspPI	GGATC	2 cut(s)	135, 571
BspQI	GCTCTTC	1 cut(s)	80
BsrI	ACTGG	1 cut(s)	243
BssMI	GATC	3 cut(s)	127, 343, 563
BssNI	GRCGYC	1 cut(s)	17
BssSI	CACGAG	1 cut(s)	551
Bst2BI	CACGAG	1 cut(s)	551
Bst4CI	ACNGT	1 cut(s)	418
Bst6I	CTCTTC	2 cut(s)	80, 292
BstACI	GRCGYC	1 cut(s)	17
BstC8I	GCNNGC	2 cut(s)	92, 198
BstDEI	CTNAG	1 cut(s)	558
BstF5I	GGATG	2 cut(s)	441, 464
BstKTI	GATC	3 cut(s)	130, 346, 566
BstMAI	GTCTC	1 cut(s)	554
BstMBI	GATC	3 cut(s)	127, 343, 563
BstNSI	RCATGY	1 cut(s)	170
BstV1I	GCAGC	2 cut(s)	42, 45
BstX2I	RGATCY	1 cut(s)	127
BstYI	RGATCY	1 cut(s)	127
BtsCI	GGATG	2 cut(s)	441, 464
BtsI	GCAGTG	2 cut(s)	484, 512
BtsIMutI	CAGTG	2 cut(s)	484, 512
BveI	ACCTGC	1 cut(s)	560
Cac8I	GCNNGC	2 cut(s)	92, 198
CviAII	CATG	1 cut(s)	167
CviJI	RGCY	8 cut(s)	55, 196, 215, 263, 520, 557, 602, 630
CviKI_1	RGCY	8 cut(s)	55, 196, 215, 263, 520, 557, 602, 630
DdeI	CTNAG	1 cut(s)	558
DpnI	GATC	3 cut(s)	129, 345, 565
DpnII	GATC	3 cut(s)	127, 343, 563
Eam1104I	CTCTTC	2 cut(s)	80, 292
EarI	CTCTTC	2 cut(s)	80, 292
Ecl136II	GAGCTC	1 cut(s)	263
Eco24I	GRGCYC	1 cut(s)	265
Eco53kI	GAGCTC	1 cut(s)	263
Eco88I	CYCGRG	2 cut(s)	73, 489
EcoICRI	GAGCTC	1 cut(s)	263
EcoT38I	GRGCYC	1 cut(s)	265
FaeI	CATG	1 cut(s)	170
FaiI	YATR	6 cut(s)	168, 250, 285, 326, 328, 456
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	2 cut(s)	83, 221
FatI	CATG	1 cut(s)	166
Fnu4HI	GCNGC	2 cut(s)	56, 59
FokI	GGATG	2 cut(s)	448, 471
FriOI	GRGCYC	1 cut(s)	265
Fsp4HI	GCNGC	2 cut(s)	56, 59
FspBI	CTAG	2 cut(s)	357, 447
GluI	GCNGC	2 cut(s)	56, 59
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	1 cut(s)	170
HindIII	AAGCTT	1 cut(s)	194
HinfI	GANTC	5 cut(s)	5, 29, 71, 547, 617
HphI	GGTGA	1 cut(s)	38
Hpy188I	TCNGA	4 cut(s)	34, 115, 322, 613
Hpy188III	TCNNGA	4 cut(s)	100, 362, 373, 470
Hpy99I	CGWCG	2 cut(s)	19, 22
HpyAV	CCTTC	1 cut(s)	130
HpyCH4III	ACNGT	1 cut(s)	418
HpyCH4IV	ACGT	1 cut(s)	17
HpyCH4V	TGCA	5 cut(s)	11, 200, 369, 477, 482
HpyF3I	CTNAG	1 cut(s)	558
HpySE526I	ACGT	1 cut(s)	17
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	1 cut(s)	170
Kzo9I	GATC	3 cut(s)	127, 343, 563
LguI	GCTCTTC	1 cut(s)	80
LmnI	GCTCC	4 cut(s)	99, 260, 517, 607
LpnPI	CCDG	5 cut(s)	9, 201, 206, 256, 555
Lsp1109I	GCAGC	2 cut(s)	42, 45
LweI	GCATC	3 cut(s)	366, 378, 449
MaeI	CTAG	2 cut(s)	357, 447
MaeII	ACGT	1 cut(s)	17
MaeIII	GTNAC	2 cut(s)	169, 289
MalI	GATC	3 cut(s)	129, 345, 565
MboI	GATC	3 cut(s)	127, 343, 563
MboII	GAAGA	3 cut(s)	97, 309, 580
MflI	RGATCY	1 cut(s)	127
MhlI	GDGCHC	2 cut(s)	92, 265
MluCI	AATT	4 cut(s)	188, 201, 313, 440
MlyI	GAGTC	3 cut(s)	38, 65, 556
MmeI	TCCRAC	4 cut(s)	12, 138, 161, 364
MnlI	CCTC	7 cut(s)	107, 118, 251, 424, 508, 553, 615
MroXI	GAANNNNTTC	1 cut(s)	587
MseI	TTAA	2 cut(s)	156, 527
MslI	CAYNNNNRTG	1 cut(s)	374
MspA1I	CMGCKG	1 cut(s)	215
NdeII	GATC	3 cut(s)	127, 343, 563
NlaIII	CATG	1 cut(s)	170
NmuCI	GTSAC	2 cut(s)	169, 289
NspI	RCATGY	1 cut(s)	170
PaeR7I	CTCGAG	1 cut(s)	489
PaqCI	CACCTGC	1 cut(s)	560
PciSI	GCTCTTC	1 cut(s)	80
PdmI	GAANNNNTTC	1 cut(s)	587
PfeI	GAWTC	2 cut(s)	5, 617
PflFI	GACNNNGTC	1 cut(s)	19
PkrI	GCNGC	2 cut(s)	57, 60
PleI	GAGTC	3 cut(s)	37, 65, 555
PpsI	GAGTC	3 cut(s)	37, 65, 555
Psp124BI	GAGCTC	1 cut(s)	265
PspXI	VCTCGAGB	1 cut(s)	489
PsuI	RGATCY	1 cut(s)	127
PsyI	GACNNNGTC	1 cut(s)	19
PvuII	CAGCTG	1 cut(s)	215
RseI	CAYNNNNRTG	1 cut(s)	374
SacI	GAGCTC	1 cut(s)	265
SapI	GCTCTTC	1 cut(s)	80
SaqAI	TTAA	2 cut(s)	156, 527
SatI	GCNGC	2 cut(s)	56, 59
Sau3AI	GATC	3 cut(s)	127, 343, 563
SchI	GAGTC	3 cut(s)	38, 65, 556
SduI	GDGCHC	2 cut(s)	92, 265
SetI	ASST	9 cut(s)	20, 57, 122, 198, 217, 265, 522, 574, 604
SfaNI	GCATC	3 cut(s)	366, 378, 449
Sfr274I	CTCGAG	1 cut(s)	489
SlaI	CTCGAG	1 cut(s)	489
SmiMI	CAYNNNNRTG	1 cut(s)	374
SmlI	CTYRAG	4 cut(s)	100, 264, 470, 489
SmoI	CTYRAG	4 cut(s)	100, 264, 470, 489
SpeI	ACTAGT	1 cut(s)	356
Sse9I	AATT	4 cut(s)	188, 201, 313, 440
SspMI	CTAG	2 cut(s)	357, 447
SstI	GAGCTC	1 cut(s)	265
TaaI	ACNGT	1 cut(s)	418
TaiI	ACGT	1 cut(s)	20
TaqI	TCGA	4 cut(s)	39, 123, 396, 490
TasI	AATT	4 cut(s)	188, 201, 313, 440
TfiI	GAWTC	2 cut(s)	5, 617
Tru1I	TTAA	2 cut(s)	156, 527
Tru9I	TTAA	2 cut(s)	156, 527
TscAI	CASTG	2 cut(s)	484, 512
TseFI	GTSAC	2 cut(s)	169, 289
TseI	GCWGC	2 cut(s)	55, 58
Tsp45I	GTSAC	2 cut(s)	169, 289
TspDTI	ATGAA	2 cut(s)	237, 630
TspRI	CASTG	2 cut(s)	484, 512
Tth111I	GACNNNGTC	1 cut(s)	19
XapI	RAATTY	2 cut(s)	313, 440
XceI	RCATGY	1 cut(s)	170
XhoI	CTCGAG	1 cut(s)	489
XmnI	GAANNNNTTC	1 cut(s)	587
XspI	CTAG	2 cut(s)	357, 447
ZraI	GACGTC	1 cut(s)	18

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.