MD01G1008900.v1.1

SAL1 phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008514
Physical Location & Seq
Reverse (-)
5704008 .. 5713216
9209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1008900.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGTCCTACGAAAAGGAGCTCGCTGCTGCCAAAAAGGCTGCCTCACTCGCTGCTCTCCTCTGCCAGGCAATACTCTCTCTCTCTGATTTCATCACTTTTTGTTTGAGAGTTGACTTTAAGAATGGAAACCCAGAGCATAAATTGTCGATTGAGTCCGCTTCGAATTCGGAGCATGATAATGTAATTTGCGACCAACTCAGAATGCAAAACATTTGTTTGAGCAAAACCCATGTCGTGAATTTGCCAATTCTTTTGCCATTTAAGCATTTATTGGACAATGAATCCGCCGTTGTATTTGTTTACCATCTCCAAGAAACAGAAAGCTTGTATTTCAACCCAGATTGCTCATTAAGTAGACCCATGGCCAATCAATATAATCTGTCATCCCAAGTTGACTTCAGTTATTGGTTTTCTCTGCTTCAGAAGGTGCAAAAGGCCCTATTGCAGTCAGATGTTCATTCAAAATCAGATAAAAGTCCAGTTACAGTGGCGGATTACGGTTCACAGGCTATTGTGAGTTTGGTACTGGAAAGGGAACTTCCTTGTGAATCGTTCTCGCTTGTGGCCGAGGAGGACTCAGGAGATCTTCGGAAAGAAAGTGGGCAGGAAACACTAGAGCGCATAAAAAAACTTGTTAATGATACTCTTGCCAACAGTGACTTTTCCAGTGTTTCTAATTTAACCACTGAAGATGTGCTCAGAGCTATTGACAATGGGAAATCTGAAGGTGGTTCTAGCGGCCAGCACTGGGTTCTAGATCCCATAGACGGAACCAAAGGGTTTGTTAGAGGAGATCAATATGCCATAGCATTGGGATTGCTGGATGAAGGAAAAGTTGTGTTGGGTGTCCTGGCCTGTCCAAATCTTCCTCTTGCATCCATTAGTGGCAATAATCAGCATTCTTCATATGATAAAGTTGGTTGTCTTTTCTTTGCTACAGTTGGTACCGGAACATATATGCAGCCATTGGATGGTTCGTACCCGATAAAGGTGCATGTCAGCGCTACTGAAAATCCTGAAGAAGCTTCGTTTTTCGAATCTTTTGAAGCAGCACACTCCTTGCATGACTTGTCTAGCAGCATTGCTCAGAAACTTGGTGTCAAAGCCTTGCCAGTTAGAATTGACAGCCAGGCAAAGTATGGAGCACTTTCCAGAGGAGATGGAGCAATATATCTTCGTTTCCCCCATAAAGGATATCGTGAAAAAATATGGGATCATGCTGCTGGGTGCATTGTCGTGACTGAAGCTGGGGGTGTGGTCGCAGATGCTGCGGGGAACCCGTTGGATTTCTCAAAGGGGAAGTATCTTGATCTTGATACGGGCATAATCGTCACAAATCAGAAACTAATGCCATTACTATTGAAGGCAGTTAGAGAATCCCTCGAAGAAAAACTTTCATCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko00920 ko01100 ko01120 ko04070 map00562 map00920 map01100 map01120 map04070
Pfam Domains
Protein Families
Inositol_P

Protein Analysis

469

Amino Acids

51.03

Weight (kDa)

5.23

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 157 - 460 1.8e-48 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 944
AccB1I GGYRCC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 971
AccI GTMKAC 1 cut(s) 355
AciI CCGC 5 cut(s) 156, 285, 491, 738, 1271
AclWI GGATC 2 cut(s) 752, 1221
AcoI YGGCCR 3 cut(s) 363, 564, 739
AcsI RAATTY 2 cut(s) 163, 238
AcuI CTGAAG 6 cut(s) 382, 404, 708, 744, 1038, 1263
AfaI GTAC 3 cut(s) 525, 946, 980
AfeI AGCGCT 1 cut(s) 1003
AfiI CCNNNNNNNGG 6 cut(s) 64, 748, 767, 971, 988, 1190
AgsI TTSAA 4 cut(s) 334, 462, 1046, 1363
AjnI CCWGG 3 cut(s) 63, 849, 1128
AluBI AGCT 5 cut(s) 19, 324, 704, 1025, 1247
AluI AGCT 5 cut(s) 19, 324, 704, 1025, 1247
Alw21I GWGCWC 3 cut(s) 21, 699, 1147
AlwI GGATC 2 cut(s) 752, 1221
AlwNI CAGNNNCTG 1 cut(s) 1268
Aor51HI AGCGCT 1 cut(s) 1003
AoxI GGCC 5 cut(s) 363, 435, 564, 739, 852
ApeKI GCWGC 9 cut(s) 23, 26, 38, 50, 961, 1049, 1077, 1220, 1268
ApoI RAATTY 2 cut(s) 163, 238
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp718I GGTACC 1 cut(s) 944
AspLEI GCGC 2 cut(s) 621, 1004
AspS9I GGNCC 1 cut(s) 436
AsuII TTCGAA 2 cut(s) 161, 1035
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 1 cut(s) 944
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 3 cut(s) 21, 699, 1147
BbvI GCAGC 9 cut(s) 10, 13, 25, 37, 973, 1061, 1089, 1207, 1255
BccI CCATC 3 cut(s) 312, 965, 1154
BceAI ACGGC 1 cut(s) 272
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 3 cut(s) 65, 851, 1130
BfaI CTAG 4 cut(s) 614, 735, 755, 1074
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 1005
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 583
Bme1390I CCNGG 3 cut(s) 65, 851, 1130
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 3 cut(s) 772, 946, 1277
BmrFI CCNGG 3 cut(s) 65, 851, 1130
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 2 cut(s) 884, 1255
BmuI ACTGGG 1 cut(s) 757
BplI GAGNNNNNCTC 4 cut(s) 560, 592, 1365, 1397
Bpu14I TTCGAA 2 cut(s) 161, 1035
BsaJI CCNNGG 2 cut(s) 360, 567
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 748, 767, 971, 988, 1190
Bse1I ACTGG 5 cut(s) 479, 531, 666, 752, 1112
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 3 cut(s) 65, 851, 1130
BseDI CCNNGG 2 cut(s) 360, 567
BseGI GGATG 4 cut(s) 383, 829, 875, 976
BseLI CCNNNNNNNGG 6 cut(s) 64, 748, 767, 971, 988, 1190
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 4 cut(s) 211, 591, 712, 1100
BseNI ACTGG 5 cut(s) 479, 531, 666, 752, 1112
BseRI GAGGAG 4 cut(s) 47, 584, 804, 1170
BseXI GCAGC 9 cut(s) 10, 13, 25, 37, 973, 1061, 1089, 1207, 1255
BseYI CCCAGC 2 cut(s) 1223, 1247
BshFI GGCC 5 cut(s) 365, 437, 566, 741, 854
BshNI GGYRCC 1 cut(s) 944
BsiHKAI GWGCWC 3 cut(s) 21, 699, 1147
BsiSI CCGG 1 cut(s) 948
BslI CCNNNNNNNGG 6 cut(s) 64, 748, 767, 971, 988, 1190
BsmI GAATGC 2 cut(s) 207, 898
BsnI GGCC 5 cut(s) 365, 437, 566, 741, 854
Bsp119I TTCGAA 2 cut(s) 161, 1035
Bsp1286I GDGCHC 3 cut(s) 21, 699, 1147
Bsp143I GATC 5 cut(s) 583, 757, 793, 1213, 1309
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 5 cut(s) 156, 285, 491, 738, 1271
BspANI GGCC 5 cut(s) 365, 437, 566, 741, 854
BspCNI CTCAG 4 cut(s) 210, 590, 711, 1099
BspLI GGNNCC 3 cut(s) 772, 946, 1277
BspPI GGATC 2 cut(s) 752, 1221
BspT104I TTCGAA 2 cut(s) 161, 1035
BspT107I GGYRCC 1 cut(s) 944
BsrDI GCAATG 1 cut(s) 1080
BsrI ACTGG 5 cut(s) 479, 531, 666, 752, 1112
BssECI CCNNGG 2 cut(s) 360, 567
BssMI GATC 5 cut(s) 583, 757, 793, 1213, 1309
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 3 cut(s) 65, 851, 1130
Bst4CI ACNGT 4 cut(s) 487, 500, 656, 940
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstBI TTCGAA 2 cut(s) 161, 1035
BstC8I GCNNGC 2 cut(s) 21, 743
BstDEI CTNAG 4 cut(s) 197, 577, 698, 1086
BstDSI CCRYGG 1 cut(s) 360
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 4 cut(s) 383, 829, 875, 976
BstH2I RGCGCY 1 cut(s) 1005
BstHHI GCGC 2 cut(s) 621, 1004
BstKTI GATC 5 cut(s) 586, 760, 796, 1216, 1312
BstMBI GATC 5 cut(s) 583, 757, 793, 1213, 1309
BstMWI GCNNNNNNNGC 4 cut(s) 35, 47, 262, 1268
BstNI CCWGG 3 cut(s) 65, 851, 1130
BstNSI RCATGY 1 cut(s) 998
BstSCI CCNGG 3 cut(s) 63, 849, 1128
BstSFI CTRYAG 1 cut(s) 936
BstV1I GCAGC 9 cut(s) 10, 13, 25, 37, 973, 1061, 1089, 1207, 1255
BstX2I RGATCY 2 cut(s) 583, 757
BstXI CCANNNNNNTGG 1 cut(s) 811
BstYI RGATCY 2 cut(s) 583, 757
BsuRI GGCC 5 cut(s) 365, 437, 566, 741, 854
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 4 cut(s) 383, 829, 875, 976
BtsIMutI CAGTG 5 cut(s) 492, 661, 673, 684, 745
Cac8I GCNNGC 2 cut(s) 21, 743
CaiI CAGNNNCTG 1 cut(s) 1268
CfoI GCGC 2 cut(s) 621, 1004
Cfr13I GGNCC 1 cut(s) 436
Csp6I GTAC 3 cut(s) 524, 945, 979
CviAII CATG 6 cut(s) 173, 230, 361, 995, 1064, 1217
CviQI GTAC 3 cut(s) 524, 945, 979
DdeI CTNAG 4 cut(s) 197, 577, 698, 1086
DpnI GATC 5 cut(s) 585, 759, 795, 1215, 1311
DpnII GATC 5 cut(s) 583, 757, 793, 1213, 1309
EaeI YGGCCR 3 cut(s) 363, 564, 739
EciI GGCGGA 2 cut(s) 274, 506
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 1196
Eco47III AGCGCT 1 cut(s) 1003
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 6 cut(s) 382, 404, 708, 744, 1038, 1263
EcoICRI GAGCTC 1 cut(s) 19
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 3 cut(s) 63, 849, 1128
EcoRV GATATC 1 cut(s) 1196
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 6 cut(s) 176, 233, 364, 998, 1067, 1220
FalI AAGNNNNNCTT 1 cut(s) 1377
FatI CATG 6 cut(s) 172, 229, 360, 994, 1063, 1216
FauI CCCGC 1 cut(s) 1264
FauNDI CATATG 1 cut(s) 907
FblI GTMKAC 1 cut(s) 355
FokI GGATG 4 cut(s) 370, 836, 862, 983
FriOI GRGCYC 1 cut(s) 21
FspBI CTAG 4 cut(s) 614, 735, 755, 1074
GlaI GCGC 2 cut(s) 620, 1003
GsaI CCCAGC 2 cut(s) 1227, 1251
HaeII RGCGCY 1 cut(s) 1005
HaeIII GGCC 5 cut(s) 365, 437, 566, 741, 854
HapII CCGG 1 cut(s) 948
HhaI GCGC 2 cut(s) 621, 1004
Hin1II CATG 6 cut(s) 176, 233, 364, 998, 1067, 1220
Hin6I GCGC 2 cut(s) 619, 1002
HinP1I GCGC 2 cut(s) 619, 1002
HincII GTYRAC 2 cut(s) 112, 394
HindII GTYRAC 2 cut(s) 112, 394
HindIII AAGCTT 2 cut(s) 322, 1023
HinfI GANTC 6 cut(s) 152, 281, 548, 575, 1037, 1376
HpaII CCGG 1 cut(s) 948
Hpy166II GTNNAC 5 cut(s) 112, 301, 356, 394, 503
Hpy188III TCNNGA 9 cut(s) 235, 579, 755, 1016, 1152, 1199, 1237, 1307, 1313
Hpy8I GTNNAC 5 cut(s) 112, 301, 356, 394, 503
HpyAV CCTTC 4 cut(s) 418, 719, 821, 1357
HpyCH4III ACNGT 4 cut(s) 487, 500, 656, 940
HpyCH4V TGCA 8 cut(s) 205, 430, 445, 875, 961, 994, 1063, 1230
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 47, 262, 1268
HpyF3I CTNAG 4 cut(s) 197, 577, 698, 1086
Hsp92II CATG 6 cut(s) 176, 233, 364, 998, 1067, 1220
HspAI GCGC 2 cut(s) 619, 1002
KpnI GGTACC 1 cut(s) 948
Kzo9I GATC 5 cut(s) 583, 757, 793, 1213, 1309
LmnI GCTCC 4 cut(s) 16, 169, 1142, 1163
Lsp1109I GCAGC 9 cut(s) 10, 13, 25, 37, 973, 1061, 1089, 1207, 1255
LweI GCATC 2 cut(s) 884, 1255
MaeI CTAG 4 cut(s) 614, 735, 755, 1074
MaeIII GTNAC 4 cut(s) 481, 656, 1237, 1330
MalI GATC 5 cut(s) 585, 759, 795, 1215, 1311
MboI GATC 5 cut(s) 583, 757, 793, 1213, 1309
MboII GAAGA 7 cut(s) 578, 701, 857, 894, 1031, 1166, 1397
MflI RGATCY 2 cut(s) 583, 757
MhlI GDGCHC 3 cut(s) 21, 699, 1147
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 7 cut(s) 140, 163, 183, 238, 246, 676, 1119
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 2 cut(s) 161, 569
MmeI TCCRAC 1 cut(s) 1263
MnlI CCTC 8 cut(s) 52, 68, 562, 565, 782, 879, 1148, 1391
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
MseI TTAA 5 cut(s) 117, 261, 350, 636, 680
MslI CAYNNNNRTG 3 cut(s) 177, 1235, 1402
Msp20I TGGCCA 1 cut(s) 365
MspI CCGG 1 cut(s) 948
MspR9I CCNGG 3 cut(s) 65, 851, 1130
Mva1269I GAATGC 2 cut(s) 207, 898
MvaI CCWGG 3 cut(s) 65, 851, 1130
MwoI GCNNNNNNNGC 4 cut(s) 35, 47, 262, 1268
NcoI CCATGG 1 cut(s) 360
NdeI CATATG 1 cut(s) 907
NdeII GATC 5 cut(s) 583, 757, 793, 1213, 1309
NlaIII CATG 6 cut(s) 176, 233, 364, 998, 1067, 1220
NlaIV GGNNCC 3 cut(s) 772, 946, 1277
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 3 cut(s) 656, 1237, 1330
NspI RCATGY 1 cut(s) 998
NspV TTCGAA 2 cut(s) 161, 1035
PctI GAATGC 2 cut(s) 207, 898
PfeI GAWTC 4 cut(s) 281, 548, 1037, 1376
PflMI CCANNNNNTGG 1 cut(s) 971
PleI GAGTC 2 cut(s) 160, 569
PpsI GAGTC 2 cut(s) 160, 569
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 63, 849, 1128
PspFI CCCAGC 2 cut(s) 1223, 1247
PspGI CCWGG 3 cut(s) 63, 849, 1128
PspN4I GGNNCC 3 cut(s) 772, 946, 1277
PspPI GGNCC 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 1268
PsuI RGATCY 2 cut(s) 583, 757
RsaI GTAC 3 cut(s) 525, 946, 980
RsaNI GTAC 3 cut(s) 524, 945, 979
RseI CAYNNNNRTG 3 cut(s) 177, 1235, 1402
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 5 cut(s) 117, 261, 350, 636, 680
Sau3AI GATC 5 cut(s) 583, 757, 793, 1213, 1309
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 2 cut(s) 161, 569
ScrFI CCNGG 3 cut(s) 65, 851, 1130
SduI GDGCHC 3 cut(s) 21, 699, 1147
SetI ASST 8 cut(s) 21, 326, 429, 706, 730, 993, 1027, 1249
SfaNI GCATC 2 cut(s) 884, 1255
SfcI CTRYAG 1 cut(s) 936
SfuI TTCGAA 2 cut(s) 161, 1035
SmiMI CAYNNNNRTG 3 cut(s) 177, 1235, 1402
Sse9I AATT 7 cut(s) 140, 163, 183, 238, 246, 676, 1119
SsiI CCGC 5 cut(s) 156, 285, 491, 738, 1271
SspMI CTAG 4 cut(s) 614, 735, 755, 1074
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 3 cut(s) 63, 849, 1128
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 4 cut(s) 487, 500, 656, 940
TaqI TCGA 4 cut(s) 146, 161, 1035, 1383
TasI AATT 7 cut(s) 140, 163, 183, 238, 246, 676, 1119
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 4 cut(s) 281, 548, 1037, 1376
Tru1I TTAA 5 cut(s) 117, 261, 350, 636, 680
Tru9I TTAA 5 cut(s) 117, 261, 350, 636, 680
TscAI CASTG 5 cut(s) 492, 661, 673, 691, 752
TseFI GTSAC 3 cut(s) 656, 1237, 1330
TseI GCWGC 9 cut(s) 23, 26, 38, 50, 961, 1049, 1077, 1220, 1268
Tsp45I GTSAC 3 cut(s) 656, 1237, 1330
TspDTI ATGAA 6 cut(s) 79, 294, 446, 840, 894, 1386
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 5 cut(s) 492, 661, 673, 691, 752
Van91I CCANNNNNTGG 1 cut(s) 971
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 163, 238
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 1 cut(s) 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XmiI GTMKAC 1 cut(s) 355
XspI CTAG 4 cut(s) 614, 735, 755, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.