MD01G1015800.v1.1

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010504
Physical Location & Seq
Reverse (-)
7243220 .. 7249756
6537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1015800.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGAAGTTTGACCGGGAGGCAAATTTGAAGTGTTTGATTGGGCAATTCCAACTAGGGTTTAAGACTATTTCTGGTGCAAGCTTGATTTCATCTGTGGCTGCTCTTCTGGGTATTGCTGGTGGCTTTGTTCTTGCTCTCAAAGGCTCGAAATTCGGCATCCTAAACACCTTACACTTAGAGTGGCTGCCAAACACTCAGGCTTCTTCTCGCAAGCAGCTGCCTTTGGTACCCGGCCTCCAAAATCTTGGAAATAATTGTTTCCTAAATGTTATTTTACAGGCTCTGGCTAGCTGTTCATACTTTCAACCCTTTCTCCAGAATGTCTTAGAGGAATATGAGTTCTCAGCAGTTCAGGATCGAGTTGATGCCTTTCCTCTTACGATAGCATTGGCAGCTTTGTTAGAAGATCTAAGTGTAGTTGGTGGAGGAAGAGTTGTATTAAGTCCGCGAAAATTGATGCTTGCGATGGAAGCTTACATTCAGAATTTCGATCTTACAAGCCAGCAGGATGCAGCAGAAGCATTTCTTCATCTTCTGTCCTCTTTAAGAGAAGAATTTTCAGATTGTTATCTTCCAAATCAATGTTCTTTAGCAGAACTCTTTGCCTCTAATTGTAGGATTATTACTCCAAAGAGAATGGAGAACCAGAGTGAGCAGGCAAGATGGCAGCAACAGTACCTTGGGCCATTTGATGGAATTCTCAGTAGCATCCTCACGTGTCGAAGTTGCTCAACACAGATATCATTGAACTTTGAATCTTTTCACAGCTTGCCTCTTTCACCAGTCCTTAATGGTGGTGCCACAATTATGGTTGGATGCACCTTAGAGGATTGCCTGGAGCAGTTCACTGCTGCTGAACAAGTTGAGAATTATCACTGTAACTATTGTTGGCACATTGCGGCAGTCAAGTATTTATTTTCTATGGGAGTAAATGAGGAAGAGATTGAAAAGATTAAGAGATGCCCTGAGCAAGATTCCTGTGACTGTCGAAGACATTTTCATCTACATAAGCTACCTTGGTCAAATAAATTTTCATATACTCTAAAGCAAATAAGTATTGCTCGTTGCCCAAAGATTCTATGCATTCATCTAAAGCGTGTTTCAATCAATATATTTGGAGAACTAGTAAAACTTCAGGGCCATATTTCTTTTCCATTGGTTTTGGACCTTTTGAGATTCACGACAAGTGATGTGGGAATAAAGAGTTTGGAAGAAAACATGAAAAAAGGGGAAGTTCAGCAACAAAATAAAAACTCAAGAACTTTTCCCAATCAGTTCAATATGCAGTATGATCAGATAAAGCTGAATCATATCTATGGATTAACGAGAGGAAGTATCTACCCAGAAGAATCAGCTTCAGATTTGACTGAGAGTTGCAAAGTGGTTCCTTCAAATACTTGCCTCTACCGTCTTGTTGCCGTTGTGGAGCACTTTGGAAGAGCTGGAAGTGGGCACTACACCGTTTACAGAAGCGTGAGAGCTGATTTATGTGAAGAACCTTGTGAGGAGTCTGAGGTTGACGCTGCTGCTTGGTGCTGGTATTCTATTTCGGATTCAGAAGTGTGTAGGGTTTCAGAGGAAGATGTTCTTGCTGCAGAAGCAAGCTTTCTTTTCTTCGAAAAATACGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

545

Amino Acids

61.13

Weight (kDa)

5.49

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 78 - 539 2.3e-44 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 226, 797
AccB7I CCANNNNNTGG 1 cut(s) 692
AccII CGCG 1 cut(s) 448
AciI CCGC 2 cut(s) 446, 899
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 1028
AcuI CTGAAG 2 cut(s) 1118, 1341
AcvI CACGTG 1 cut(s) 717
AfaI GTAC 2 cut(s) 228, 677
AfiI CCNNNNNNNGG 1 cut(s) 692
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 8 cut(s) 28, 305, 748, 755, 947, 1104, 1279, 1392
AhlI ACTAGT 1 cut(s) 1123
AjnI CCWGG 1 cut(s) 834
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AloI GAACNNNNNNTCC 2 cut(s) 323, 355
Alw21I GWGCWC 1 cut(s) 1431
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 283
AoxI GGCC 3 cut(s) 232, 683, 1138
ApoI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 1028
Asp700I GAANNNNTTC 3 cut(s) 522, 759, 1584
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 3 cut(s) 683, 1138, 1165
AsuC2I CCSGG 2 cut(s) 14, 231
AsuHPI GGTGA 1 cut(s) 771
AsuII TTCGAA 1 cut(s) 1617
AsuNHI GCTAGC 1 cut(s) 287
AvaII GGWCC 1 cut(s) 1165
BaeGI GKGCMC 1 cut(s) 1455
BanI GGYRCC 2 cut(s) 226, 797
BbrPI CACGTG 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 997
Bbv12I GWGCWC 1 cut(s) 1431
BccI CCATC 3 cut(s) 460, 657, 686
BceAI ACGGC 1 cut(s) 1403
BciT130I CCWGG 1 cut(s) 836
BclI TGATCA 1 cut(s) 1291
BcnI CCSGG 2 cut(s) 14, 231
BcuI ACTAGT 1 cut(s) 1123
BfaI CTAG 3 cut(s) 53, 288, 1124
BfmI CTRYAG 1 cut(s) 1593
BglII AGATCT 1 cut(s) 406
Bme1390I CCNGG 3 cut(s) 14, 231, 836
Bme18I GGWCC 1 cut(s) 1165
BmgT120I GGNCC 3 cut(s) 683, 1138, 1165
BmiI GGNNCC 3 cut(s) 228, 799, 1386
BmrFI CCNGG 3 cut(s) 14, 231, 836
BmsI GCATC 7 cut(s) 165, 355, 447, 499, 717, 806, 950
BmtI GCTAGC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 997
BpmI CTGGAG 2 cut(s) 299, 857
Bpu10I CCTNAGC 1 cut(s) 966
Bpu14I TTCGAA 1 cut(s) 1617
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 2 cut(s) 14, 231
BsaAI YACGTR 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 679, 1016
BsaXI ACNNNNNCTCC 8 cut(s) 219, 249, 297, 327, 417, 447, 1110, 1140
Bsc4I CCNNNNNNNGG 1 cut(s) 692
Bse1I ACTGG 1 cut(s) 782
Bse3DI GCAATG 1 cut(s) 894
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 836
BseDI CCNNGG 2 cut(s) 679, 1016
BseGI GGATG 4 cut(s) 156, 514, 708, 821
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 1 cut(s) 692
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 6 cut(s) 209, 357, 715, 957, 1359, 1503
BseNI ACTGG 1 cut(s) 782
BseRI GAGGAG 1 cut(s) 1520
BseSI GKGCMC 1 cut(s) 1455
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 3 cut(s) 234, 685, 1140
BshNI GGYRCC 2 cut(s) 226, 797
BsiHKAI GWGCWC 1 cut(s) 1431
BsiSI CCGG 2 cut(s) 13, 231
BslI CCNNNNNNNGG 1 cut(s) 692
BsmI GAATGC 1 cut(s) 1083
BsnI GGCC 3 cut(s) 234, 685, 1140
Bsp119I TTCGAA 1 cut(s) 1617
Bsp1286I GDGCHC 2 cut(s) 1431, 1455
Bsp143I GATC 4 cut(s) 355, 406, 490, 1291
BspACI CCGC 2 cut(s) 446, 899
BspANI GGCC 3 cut(s) 234, 685, 1140
BspCNI CTCAG 6 cut(s) 208, 356, 714, 958, 1360, 1504
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 3 cut(s) 228, 799, 1386
BspMAI CTGCAG 1 cut(s) 1597
BspOI GCTAGC 1 cut(s) 291
BspPI GGATC 1 cut(s) 363
BspQI GCTCTTC 2 cut(s) 108, 1432
BspT104I TTCGAA 1 cut(s) 1617
BspT107I GGYRCC 2 cut(s) 226, 797
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 1 cut(s) 782
BssECI CCNNGG 2 cut(s) 679, 1016
BssMI GATC 4 cut(s) 355, 406, 490, 1291
BssT1I CCWWGG 2 cut(s) 679, 1016
Bst2UI CCWGG 1 cut(s) 836
Bst4CI ACNGT 5 cut(s) 675, 878, 986, 1409, 1462
Bst6I CTCTTC 4 cut(s) 108, 424, 933, 1432
BstBAI YACGTR 1 cut(s) 717
BstBI TTCGAA 1 cut(s) 1617
BstC8I GCNNGC 8 cut(s) 79, 212, 289, 462, 503, 657, 770, 1603
BstF5I GGATG 4 cut(s) 156, 514, 708, 821
BstFNI CGCG 1 cut(s) 448
BstKTI GATC 4 cut(s) 358, 409, 493, 1294
BstMBI GATC 4 cut(s) 355, 406, 490, 1291
BstMWI GCNNNNNNNGC 4 cut(s) 392, 470, 518, 1598
BstNI CCWGG 1 cut(s) 836
BstSCI CCNGG 3 cut(s) 12, 229, 834
BstSFI CTRYAG 1 cut(s) 1593
BstSLI GKGCMC 1 cut(s) 1455
BstUI CGCG 1 cut(s) 448
BstV2I GAAGAC 1 cut(s) 997
BstX2I RGATCY 1 cut(s) 406
BstXI CCANNNNNNTGG 2 cut(s) 245, 808
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 3 cut(s) 234, 685, 1140
BtgZI GCGATG 1 cut(s) 479
BtsCI GGATG 4 cut(s) 156, 514, 708, 821
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 2 cut(s) 846, 874
Cac8I GCNNGC 8 cut(s) 79, 212, 289, 462, 503, 657, 770, 1603
CaiI CAGNNNCTG 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 683, 1138, 1165
CseI GACGC 1 cut(s) 1529
Csp6I GTAC 2 cut(s) 227, 676
CspCI CAANNNNNGTGG 2 cut(s) 1173, 1208
CviAII CATG 1 cut(s) 1219
CviQI GTAC 2 cut(s) 227, 676
DpnI GATC 4 cut(s) 357, 408, 492, 1293
DpnII GATC 4 cut(s) 355, 406, 490, 1291
Eam1104I CTCTTC 4 cut(s) 108, 424, 933, 1432
EarI CTCTTC 4 cut(s) 108, 424, 933, 1432
Eco130I CCWWGG 2 cut(s) 679, 1016
Eco32I GATATC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 1165
Eco57I CTGAAG 2 cut(s) 1118, 1341
Eco72I CACGTG 1 cut(s) 717
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 1 cut(s) 834
EcoRV GATATC 1 cut(s) 741
EcoT14I CCWWGG 2 cut(s) 679, 1016
EcoT22I ATGCAT 1 cut(s) 1085
ErhI CCWWGG 2 cut(s) 679, 1016
FaeI CATG 1 cut(s) 1222
FalI AAGNNNNNCTT 4 cut(s) 510, 542, 1572, 1604
FatI CATG 1 cut(s) 1218
FbaI TGATCA 1 cut(s) 1291
FokI GGATG 4 cut(s) 143, 521, 695, 828
FspBI CTAG 3 cut(s) 53, 288, 1124
GsuI CTGGAG 2 cut(s) 299, 857
HaeIII GGCC 3 cut(s) 234, 685, 1140
HapII CCGG 2 cut(s) 13, 231
HgaI GACGC 1 cut(s) 1529
Hin1II CATG 1 cut(s) 1222
HincII GTYRAC 1 cut(s) 1519
HindII GTYRAC 1 cut(s) 1519
HindIII AAGCTT 3 cut(s) 79, 471, 1603
HinfI GANTC 8 cut(s) 755, 974, 1075, 1176, 1306, 1349, 1508, 1553
HpaII CCGG 2 cut(s) 13, 231
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 3 cut(s) 846, 1465, 1519
Hpy188I TCNGA 8 cut(s) 483, 562, 1296, 1360, 1513, 1552, 1558, 1576
Hpy188III TCNNGA 4 cut(s) 316, 353, 1180, 1257
Hpy8I GTNNAC 3 cut(s) 846, 1465, 1519
HpyAV CCTTC 2 cut(s) 1398, 1622
HpyCH4III ACNGT 5 cut(s) 675, 878, 986, 1409, 1462
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 7 cut(s) 77, 512, 819, 1083, 1285, 1377, 1595
HpyF10VI GCNNNNNNNGC 4 cut(s) 392, 470, 518, 1598
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 1 cut(s) 1222
KpnI GGTACC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 1291
Kzo9I GATC 4 cut(s) 355, 406, 490, 1291
LguI GCTCTTC 2 cut(s) 108, 1432
LmnI GCTCC 2 cut(s) 838, 1426
LweI GCATC 7 cut(s) 165, 355, 447, 499, 717, 806, 950
MaeI CTAG 3 cut(s) 53, 288, 1124
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 2 cut(s) 878, 980
MalI GATC 4 cut(s) 357, 408, 492, 1293
MboI GATC 4 cut(s) 355, 406, 490, 1291
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 1431, 1455
MlyI GAGTC 1 cut(s) 1517
MmeI TCCRAC 2 cut(s) 73, 793
Mph1103I ATGCAT 1 cut(s) 1085
MroXI GAANNNNTTC 3 cut(s) 522, 759, 1584
MseI TTAA 6 cut(s) 60, 440, 545, 789, 954, 1322
MslI CAYNNNNRTG 2 cut(s) 806, 1314
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 2 cut(s) 13, 231
MspR9I CCNGG 3 cut(s) 14, 231, 836
Mva1269I GAATGC 1 cut(s) 1083
MvaI CCWGG 1 cut(s) 836
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 4 cut(s) 392, 470, 518, 1598
NciI CCSGG 2 cut(s) 14, 231
NdeII GATC 4 cut(s) 355, 406, 490, 1291
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 1 cut(s) 1222
NlaIV GGNNCC 3 cut(s) 228, 799, 1386
NmuCI GTSAC 1 cut(s) 980
NsiI ATGCAT 1 cut(s) 1085
NspV TTCGAA 1 cut(s) 1617
PciSI GCTCTTC 2 cut(s) 108, 1432
PcsI WCGNNNNNNNCGW 1 cut(s) 1623
PctI GAATGC 1 cut(s) 1083
PdmI GAANNNNTTC 3 cut(s) 522, 759, 1584
PfeI GAWTC 7 cut(s) 755, 974, 1075, 1176, 1306, 1349, 1553
PflMI CCANNNNNTGG 1 cut(s) 692
PleI GAGTC 1 cut(s) 1516
PmaCI CACGTG 1 cut(s) 717
PmlI CACGTG 1 cut(s) 717
PpsI GAGTC 1 cut(s) 1516
Ppu21I YACGTR 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 834
PspCI CACGTG 1 cut(s) 717
PspGI CCWGG 1 cut(s) 834
PspN4I GGNNCC 3 cut(s) 228, 799, 1386
PspPI GGNCC 3 cut(s) 683, 1138, 1165
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 228, 677
RsaNI GTAC 2 cut(s) 227, 676
RseI CAYNNNNRTG 2 cut(s) 806, 1314
SapI GCTCTTC 2 cut(s) 108, 1432
SaqAI TTAA 6 cut(s) 60, 440, 545, 789, 954, 1322
Sau3AI GATC 4 cut(s) 355, 406, 490, 1291
Sau96I GGNCC 3 cut(s) 683, 1138, 1165
SchI GAGTC 1 cut(s) 1517
ScrFI CCNGG 3 cut(s) 14, 231, 836
SduI GDGCHC 2 cut(s) 1431, 1455
SfaNI GCATC 7 cut(s) 165, 355, 447, 499, 717, 806, 950
SfcI CTRYAG 1 cut(s) 1593
SfuI TTCGAA 1 cut(s) 1617
SinI GGWCC 1 cut(s) 1165
SmiMI CAYNNNNRTG 2 cut(s) 806, 1314
SmlI CTYRAG 1 cut(s) 1255
SmoI CTYRAG 1 cut(s) 1255
SpeI ACTAGT 1 cut(s) 1123
SsiI CCGC 2 cut(s) 446, 899
SspMI CTAG 3 cut(s) 53, 288, 1124
StyD4I CCNGG 3 cut(s) 12, 229, 834
StyI CCWWGG 2 cut(s) 679, 1016
TaaI ACNGT 5 cut(s) 675, 878, 986, 1409, 1462
TaiI ACGT 1 cut(s) 719
TaqI TCGA 6 cut(s) 146, 358, 489, 721, 988, 1617
TauI GCSGC 1 cut(s) 902
TfiI GAWTC 7 cut(s) 755, 974, 1075, 1176, 1306, 1349, 1553
Tru1I TTAA 6 cut(s) 60, 440, 545, 789, 954, 1322
Tru9I TTAA 6 cut(s) 60, 440, 545, 789, 954, 1322
TscAI CASTG 2 cut(s) 853, 881
TseFI GTSAC 1 cut(s) 980
Tsp45I GTSAC 1 cut(s) 980
TspDTI ATGAA 8 cut(s) 17, 78, 285, 518, 989, 1023, 1076, 1235
TspRI CASTG 2 cut(s) 853, 881
Van91I CCANNNNNTGG 1 cut(s) 692
VpaK11BI GGWCC 1 cut(s) 1165
XapI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 1028
XmnI GAANNNNTTC 3 cut(s) 522, 759, 1584
XspI CTAG 3 cut(s) 53, 288, 1124
Zsp2I ATGCAT 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.