Rh2AG273100

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010504
Physical Location & Seq
Reverse (-)
32504859 .. 32516143
11285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG273100.1

Sequence Viewer

Length: 1647 bp
ATGAAGTTCGAGAGAGAGGTCACTCTAAAGTCATTGGCTCGCCAATTTCAACATGGGTTTAAGACGCTTTCTCGTTCTTCTGGACTTCACATATCTGTGGCTGGTCTTCTGGGTATTGCTGGTCTCGTTTTGGCTCTCAAAGACTCCAAATTGGGCAAGCTCAGCAGCTTACAGTGGCTACCCAAGAACGAACGTGGTGATCCCAAGCTGCATCTGGTACCTGGGTTGCAAAACCTTGGGAACAATTGTTTCCTGAACGTGATTTTACAGGCTCTGGCTAGTTGTTCATACTTTCAACCTTTTCTTCAAAACGTTTTAGAGGAGTCTGAGTTCTTAGCAGTTGAGGATTCCGCAGATTGCGTGCCGCTTACAATATCTTTAGCTGCATTGTTAGAAGACCTATGCGCAGTTGGTGGAGGAAGAGTTGTATTGAGTCCACGGCCAGTGATGCTTGCCATAATACATTACATTGAGAACTTTGATCTAACAAGCGAGCAGGATGCAGCAGAAGCATTTCTTCACCTTCTGTCCTCTTTAAGAGAAGAATTCTCAGATTTTTATTGTCCAACTCAAGGTTCTATAGTGGAAGTCTTAGCCTCTAATGGTAGGATTATTACTCCAAAGAGAATGGAGAGCCAAAGTGAGCAGGAAAGATGGCAGCAATGCTTCTTTGGACCATTTGATGGAATTCTTGGCAGTATCCTAACCTGTCAAAGTTGTTCATCACAGATGGCTGGATGTACCTTAGAGGATTGCCTGAAGCAGTTTACTGCTGCTGAGCAAGTTGAGAATTACCACTGCAGCCATTGTTGGCATATTGCTGCAGTCAAATATTTATTATCTATGGGAGGAAATGAGGCAGCAATTAAAAAGATTGAGAGTTGCACTGAGCAAGATTCCTGTGACTGTCAAAGACTTTTTCATCTAGAATCACTACCATGGTCGAATAGATTTTCACGCACTCTAAAGCAAATAAGTATTGCTCGCTGCCCAAAGATTTTGTGCATTCACCTAAAACGCGTTTCAATCAATGTATTTGGAGAACTTGTAAAACTACAGGGCCATATTTATTTTCCATTGGTTTTGGACCTTTTAAGTTTCATGACAAGTGGGGTGGGAATAAAGCATTGGGAGAAAAACCGGAAAACAGGGCGAGTCCGGCTACAAAGAGAAAACCCTCGGCCCAATCATTTCAATTTAGAGTGTGATCCAAGAAAGTTAAATATTGTCCGGGGACTAGCAAGAGGAAGCACCTTCCAAGAAGAATTAGCTTCAGATGTATTTCAGTTCACTGCAAATGCACAAGATTCAGCAGGAGAATTCAACTTTCCCCCAACTGGAGGCTTTCCAAAAACCATGCCTACAGGCACAAACCAGCAGAATAGGACTTGCGAATTGGTTCCTTCAGATACTTGTTTGTACCGTCTTGTTGCTGTTGTGGAGCACTTTGGAAGAGCTGGAAGTGGGCATTACACTGTTTATAGAAGTGTGGGAGCTGATTCATGTATGGAACAACCTCATGATCAATTTGACTCTTCTACTGCCCGCTGGTTTTCTATTTCAGATTTAGAGGTGTGCAGTGTTTCAAAGGAACATGTTCTTGCTGCCGAAGCTAGCTTGCTTTTCTATGAAAGGTATCAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

548

Amino Acids

61.15

Weight (kDa)

6.2

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 75 - 543 4.3e-41 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 406
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 683
AccII CGCG 1 cut(s) 1020
AciI CCGC 3 cut(s) 351, 365, 1546
AclI AACGTT 1 cut(s) 312
AclWI GGATC 2 cut(s) 194, 1202
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 3 cut(s) 545, 687, 1319
AcuI CTGAAG 3 cut(s) 779, 1257, 1389
AfaI GTAC 3 cut(s) 219, 742, 1421
AfiI CCNNNNNNNGG 4 cut(s) 572, 683, 1337, 1340
AflIII ACRYGT 2 cut(s) 1018, 1594
AgsI TTSAA 7 cut(s) 50, 296, 308, 1026, 1195, 1324, 1587
AjnI CCWGG 1 cut(s) 220
AjuI GAANNNNNNNTTGG 4 cut(s) 559, 591, 1111, 1143
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 160, 168, 208, 383, 1271, 1457, 1496, 1613, 1617
AluI AGCT 9 cut(s) 160, 168, 208, 383, 1271, 1457, 1496, 1613, 1617
Alw21I GWGCWC 1 cut(s) 1446
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 194, 1202
AlwNI CAGNNNCTG 1 cut(s) 274
AoxI GGCC 3 cut(s) 440, 1060, 1181
ApoI RAATTY 3 cut(s) 545, 687, 1319
Asp700I GAANNNNTTC 3 cut(s) 513, 1398, 1596
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 4 cut(s) 674, 1060, 1087, 1182
AsuC2I CCSGG 1 cut(s) 1232
AsuHPI GGTGA 3 cut(s) 209, 512, 1001
AsuNHI GCTAGC 1 cut(s) 1613
AvaII GGWCC 2 cut(s) 674, 1087
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanI GGYRCC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 98, 402
Bbv12I GWGCWC 1 cut(s) 1446
BccI CCATC 3 cut(s) 648, 677, 724
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 1 cut(s) 222
BciVI GTATCC 1 cut(s) 710
BclI TGATCA 1 cut(s) 1522
BcnI CCSGG 1 cut(s) 1232
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 5 cut(s) 279, 926, 1238, 1614, 1645
BfmI CTRYAG 5 cut(s) 579, 799, 822, 1055, 1362
BfuI GTATCC 1 cut(s) 710
BlpI GCTNAGC 2 cut(s) 161, 777
Bme1390I CCNGG 2 cut(s) 222, 1232
Bme18I GGWCC 2 cut(s) 674, 1087
BmgT120I GGNCC 4 cut(s) 674, 1060, 1087, 1182
BmiI GGNNCC 2 cut(s) 219, 1401
BmrFI CCNGG 2 cut(s) 222, 1232
BmsI GCATC 3 cut(s) 220, 438, 490
BmtI GCTAGC 1 cut(s) 1617
BpiI GAAGAC 2 cut(s) 98, 402
BpmI CTGGAG 1 cut(s) 1359
Bpu1102I GCTNAGC 2 cut(s) 161, 777
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 1232
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 6 cut(s) 221, 235, 437, 938, 1178, 1231
BsaWI WCCGGW 1 cut(s) 1140
BsaXI ACNNNNNCTCC 6 cut(s) 314, 344, 408, 438, 1032, 1062
Bsc4I CCNNNNNNNGG 4 cut(s) 572, 683, 1337, 1340
Bse1I ACTGG 2 cut(s) 443, 1342
Bse3DI GCAATG 1 cut(s) 668
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 6 cut(s) 221, 235, 437, 938, 1178, 1231
BseGI GGATG 2 cut(s) 505, 743
BseLI CCNNNNNNNGG 4 cut(s) 572, 683, 1337, 1340
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 5 cut(s) 175, 318, 564, 768, 879
BseNI ACTGG 2 cut(s) 443, 1342
BseRI GAGGAG 1 cut(s) 335
BsgI GTGCAG 1 cut(s) 1597
Bsh1236I CGCG 1 cut(s) 1020
BshFI GGCC 3 cut(s) 442, 1062, 1183
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 1446
BsiSI CCGG 3 cut(s) 1141, 1159, 1231
BslFI GGGAC 1 cut(s) 1248
BslI CCNNNNNNNGG 4 cut(s) 572, 683, 1337, 1340
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 1248
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 3 cut(s) 442, 1062, 1183
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 1446
Bsp143I GATC 4 cut(s) 199, 481, 1207, 1522
Bsp1720I GCTNAGC 2 cut(s) 161, 777
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 3 cut(s) 351, 365, 1546
BspANI GGCC 3 cut(s) 442, 1062, 1183
BspCNI CTCAG 5 cut(s) 174, 319, 563, 769, 880
BspFNI CGCG 1 cut(s) 1020
BspHI TCATGA 2 cut(s) 1101, 1519
BspLI GGNNCC 2 cut(s) 219, 1401
BspMAI CTGCAG 2 cut(s) 803, 826
BspOI GCTAGC 1 cut(s) 1617
BspPI GGATC 2 cut(s) 194, 1202
BspQI GCTCTTC 1 cut(s) 1447
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 668
BsrI ACTGG 2 cut(s) 443, 1342
BssECI CCNNGG 6 cut(s) 221, 235, 437, 938, 1178, 1231
BssMI GATC 4 cut(s) 199, 481, 1207, 1522
BssT1I CCWWGG 2 cut(s) 235, 938
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 4 cut(s) 174, 908, 1424, 1477
Bst6I CTCTTC 3 cut(s) 415, 1447, 1540
BstC8I GCNNGC 9 cut(s) 40, 158, 362, 453, 494, 985, 1546, 1615, 1619
BstDEI CTNAG 8 cut(s) 161, 327, 334, 550, 592, 745, 777, 888
BstDSI CCRYGG 2 cut(s) 437, 938
BstF5I GGATG 2 cut(s) 505, 743
BstFNI CGCG 1 cut(s) 1020
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 4 cut(s) 202, 484, 1210, 1525
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 4 cut(s) 199, 481, 1207, 1522
BstMWI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1159, 1610
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 1598
BstSCI CCNGG 2 cut(s) 220, 1230
BstSFI CTRYAG 5 cut(s) 579, 799, 822, 1055, 1362
BstUI CGCG 1 cut(s) 1020
BstV2I GAAGAC 2 cut(s) 98, 402
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 3 cut(s) 442, 1062, 1183
BtgI CCRYGG 2 cut(s) 437, 938
BtsCI GGATG 2 cut(s) 505, 743
BtsI GCAGTG 3 cut(s) 796, 1290, 1585
BtsIMutI CAGTG 7 cut(s) 179, 450, 796, 885, 1290, 1473, 1585
Cac8I GCNNGC 9 cut(s) 40, 158, 362, 453, 494, 985, 1546, 1615, 1619
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 2 cut(s) 1101, 1519
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 4 cut(s) 674, 1060, 1087, 1182
CseI GACGC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 218, 741, 1420
CspCI CAANNNNNGTGG 2 cut(s) 1095, 1130
CviAII CATG 7 cut(s) 53, 939, 1102, 1357, 1503, 1520, 1595
CviQI GTAC 3 cut(s) 218, 741, 1420
DdeI CTNAG 8 cut(s) 161, 327, 334, 550, 592, 745, 777, 888
DpnI GATC 4 cut(s) 201, 483, 1209, 1524
DpnII GATC 4 cut(s) 199, 481, 1207, 1522
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 3 cut(s) 415, 1447, 1540
EarI CTCTTC 3 cut(s) 415, 1447, 1540
Eco130I CCWWGG 2 cut(s) 235, 938
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 674, 1087
Eco57I CTGAAG 3 cut(s) 779, 1257, 1389
EcoRI GAATTC 3 cut(s) 545, 687, 1319
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 235, 938
ErhI CCWWGG 2 cut(s) 235, 938
FaeI CATG 7 cut(s) 56, 942, 1105, 1360, 1506, 1523, 1598
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 1 cut(s) 1248
FatI CATG 7 cut(s) 52, 938, 1101, 1356, 1502, 1519, 1594
FauI CCCGC 1 cut(s) 1553
FbaI TGATCA 1 cut(s) 1522
FokI GGATG 2 cut(s) 512, 750
FspBI CTAG 5 cut(s) 279, 926, 1238, 1614, 1645
FspI TGCGCA 1 cut(s) 406
GlaI GCGC 1 cut(s) 406
GsuI CTGGAG 1 cut(s) 1359
HaeIII GGCC 3 cut(s) 442, 1062, 1183
HapII CCGG 3 cut(s) 1141, 1159, 1231
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 407
Hin1II CATG 7 cut(s) 56, 942, 1105, 1360, 1506, 1523, 1598
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HpaII CCGG 3 cut(s) 1141, 1159, 1231
HphI GGTGA 3 cut(s) 209, 512, 1001
Hpy166II GTNNAC 3 cut(s) 437, 768, 1290
Hpy188I TCNGA 5 cut(s) 328, 553, 1276, 1408, 1564
Hpy188III TCNNGA 6 cut(s) 10, 81, 253, 926, 1102, 1520
Hpy8I GTNNAC 3 cut(s) 437, 768, 1290
HpyAV CCTTC 3 cut(s) 533, 1264, 1413
HpyCH4III ACNGT 4 cut(s) 174, 908, 1424, 1477
HpyCH4IV ACGT 3 cut(s) 193, 258, 312
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1159, 1610
HpyF3I CTNAG 8 cut(s) 161, 327, 334, 550, 592, 745, 777, 888
HpySE526I ACGT 3 cut(s) 193, 258, 312
Hsp92II CATG 7 cut(s) 56, 942, 1105, 1360, 1506, 1523, 1598
HspAI GCGC 1 cut(s) 405
KpnI GGTACC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 1522
Kzo9I GATC 4 cut(s) 199, 481, 1207, 1522
LguI GCTCTTC 1 cut(s) 1447
LmnI GCTCC 2 cut(s) 1441, 1493
LweI GCATC 3 cut(s) 220, 438, 490
MaeI CTAG 5 cut(s) 279, 926, 1238, 1614, 1645
MaeII ACGT 3 cut(s) 193, 258, 312
MaeIII GTNAC 2 cut(s) 19, 902
MalI GATC 4 cut(s) 201, 483, 1209, 1524
MboI GATC 4 cut(s) 199, 481, 1207, 1522
MfeI CAATTG 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 1446
MluI ACGCGT 1 cut(s) 1018
MlyI GAGTC 5 cut(s) 137, 332, 442, 1164, 1526
MmeI TCCRAC 1 cut(s) 590
MroXI GAANNNNTTC 3 cut(s) 513, 1398, 1596
MseI TTAA 5 cut(s) 60, 536, 867, 1094, 1220
MslI CAYNNNNRTG 2 cut(s) 95, 937
MspA1I CMGCKG 1 cut(s) 1548
MspI CCGG 3 cut(s) 1141, 1159, 1231
MspR9I CCNGG 2 cut(s) 222, 1232
MunI CAATTG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 1020
MwoI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1159, 1610
NciI CCSGG 1 cut(s) 1232
NcoI CCATGG 1 cut(s) 938
NdeII GATC 4 cut(s) 199, 481, 1207, 1522
NheI GCTAGC 1 cut(s) 1613
NlaIII CATG 7 cut(s) 56, 942, 1105, 1360, 1506, 1523, 1598
NlaIV GGNNCC 2 cut(s) 219, 1401
NmeAIII GCCGAG 1 cut(s) 1159
NmuCI GTSAC 2 cut(s) 19, 902
NsbI TGCGCA 1 cut(s) 406
NspI RCATGY 1 cut(s) 1598
PagI TCATGA 2 cut(s) 1101, 1519
PciI ACATGT 1 cut(s) 1594
PciSI GCTCTTC 1 cut(s) 1447
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 3 cut(s) 513, 1398, 1596
PfeI GAWTC 5 cut(s) 347, 896, 929, 1307, 1499
PflMI CCANNNNNTGG 1 cut(s) 683
PleI GAGTC 5 cut(s) 137, 331, 441, 1163, 1526
PpsI GAGTC 5 cut(s) 137, 331, 441, 1163, 1526
PscI ACATGT 1 cut(s) 1594
Psp1406I AACGTT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 219, 1401
PspPI GGNCC 4 cut(s) 674, 1060, 1087, 1182
PstI CTGCAG 2 cut(s) 803, 826
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 3 cut(s) 219, 742, 1421
RsaNI GTAC 3 cut(s) 218, 741, 1420
RseI CAYNNNNRTG 2 cut(s) 95, 937
SapI GCTCTTC 1 cut(s) 1447
SaqAI TTAA 5 cut(s) 60, 536, 867, 1094, 1220
Sau3AI GATC 4 cut(s) 199, 481, 1207, 1522
Sau96I GGNCC 4 cut(s) 674, 1060, 1087, 1182
SchI GAGTC 5 cut(s) 137, 332, 442, 1164, 1526
ScrFI CCNGG 2 cut(s) 222, 1232
SduI GDGCHC 1 cut(s) 1446
SfaNI GCATC 3 cut(s) 220, 438, 490
SfcI CTRYAG 5 cut(s) 579, 799, 822, 1055, 1362
SinI GGWCC 2 cut(s) 674, 1087
SmiMI CAYNNNNRTG 2 cut(s) 95, 937
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SsiI CCGC 3 cut(s) 351, 365, 1546
SspI AATATT 2 cut(s) 833, 1225
SspMI CTAG 5 cut(s) 279, 926, 1238, 1614, 1645
StyD4I CCNGG 2 cut(s) 220, 1230
StyI CCWWGG 2 cut(s) 235, 938
TaaI ACNGT 4 cut(s) 174, 908, 1424, 1477
TaiI ACGT 3 cut(s) 196, 261, 315
TaqI TCGA 2 cut(s) 9, 944
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 5 cut(s) 347, 896, 929, 1307, 1499
Tru1I TTAA 5 cut(s) 60, 536, 867, 1094, 1220
Tru9I TTAA 5 cut(s) 60, 536, 867, 1094, 1220
TscAI CASTG 7 cut(s) 179, 450, 803, 892, 1297, 1480, 1585
TseFI GTSAC 2 cut(s) 19, 902
Tsp45I GTSAC 2 cut(s) 19, 902
TspDTI ATGAA 7 cut(s) 17, 276, 711, 911, 1090, 1491, 1644
TspRI CASTG 7 cut(s) 179, 450, 803, 892, 1297, 1480, 1585
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 674, 1087
XapI RAATTY 3 cut(s) 545, 687, 1319
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 1598
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 211
XmnI GAANNNNTTC 3 cut(s) 513, 1398, 1596
XspI CTAG 5 cut(s) 279, 926, 1238, 1614, 1645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.