RchiOBHm_Chr2g0118111

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010504
Physical Location & Seq
Reverse (-)
30469344 .. 30475201
5858 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49097

Sequence Viewer

Length: 1749 bp
ATGAAGTTCGAGAGAGAGGTCACTCTAAAGTCATTGGCTCGCCAATTCCAACATGGGTTTAAGACGCTTTCTCGTTCTTCTGGACTTCACATATCTGTAGCTGGTCTTCTTGGTATTGCTGGTCTCGTTTTGGCTCTCAAAGACTCCAAATTGGGCAAGCTCAGCAGCTTACAGTGGCTACCCAAGAACGAACGTGGTGATCCCAACTTGCATTTGGTACCTGGGTTGCAAAACCTTGGGAACAATTGTTTCCTGAACGTGATTTTACAGGCTCTGGCTAGTTGTTCATACTTTCAACCTTTTCTTCAAAAGGTTTTAGAGGAGTCTGAGTTCTTAGCAGTTGAGGATTCCGCAGATTGCGTGCCGCTTACAATATCTTTAGCTGCATTGTTAGAAGACCTATGCGCAGTTGGTGGAGGAAGAGTTGTATTGAGTCCACGGCGAGTGATGCTTGCCATAACACATTACATTGAGAACTTTGATCTAACAAGCGAGCAGGATGCAGCAGAAGCATTTCTTCACCTTCTGTCCTCTTTAAGAGAAGAATTCTCAGATTTTTATTGTCCAACTCAAGGTTCTATAGTGGAAGTCTTAGCCTCTAATGGTAGGATTATTACTCCAAAGAGAACGGAGAGCCAAAGTGAGCAGGAAAGATGGCAGCAACGCTTCTTTGGACCATTTGATGGAATTCTTGGCAGTATCCTAACCTGTCGAAGTTGTTCATCGCAGATATCATTGAATTTTGAATCTTTTCACAGCTTGCCTCTTTCACCAGTGCTTGATGGTGGTGCCACAATTGTATGTGTACTGAAGACTTCACTCTTTGATATTATGGCTGGATGTACCTTAGAGGATTGCCTGAAGCAGTTTACTGCTGCTGAGCAAGTTGAGAATTACCACTGCAGCCATTGTTGGCATATTGCTGCAGTCAAATATTTATTATCTATGGGAGGAAATGAGGCAGCAATTGAAAAGATTGAGAGTTGCACTGAGCAAGATTCCTGTGACTGTCAAAGACTTTTTCATCTAGAATCACTACCATGGTCGAATAGGTTTTCACGCACTCTAAAGCAAATAAGTATTGCTCGCTGCCCAAAGATTTTGTGCATTCACCTAAAACGCGTTTCAATCAATGTATTTGGAGAACTTGTAAAACTACAGGGCCATATTTATTTTCCATTGGTTTTGGACCTTTTAAGTTTCATGACAAGTGGGGTGGGAATAAAGCATTGGGAGAAAAACCAGAAAACAGGGCGAGTCTGGCTACAAAGAGAAAACCCTCGGCCCAATCATTTCAATTTAGAGTGTGATCCAAGAAAGTTAAATATTGTCCGGGGACTAGCAAGAGGAAGCACCTTCCAAGAAGAATTAGCTTCAGATGTATCTCAGTTCACTGCAAATGCACAAGATTCAGCAGGAGAATTCAACTTTCCCCCAACTGGAGGCTTTCCAAAAACCATGCCTACAGGCACAAACCAGCAGAATAGGACTTGCGAATTGGTTCCTTCAGATACTTGTTTGTACCGTCTTGTTGCTGTTGTGGAGCACTTTGGAAGAGCTGGAAGTGGGCATTACACTGTTTATAGAAGTGTGGGAGCTGATTCATGTATGGAACAACCTCATGATCAATTTGACTCTTCTACTGCCCGCTGGTTTTCTATTTCAGATTCAGAGGTGTGCAGTGTTTCAAAGGAACATGTTCTTGCCGCAGAAGCTAGCTTGCTTTTCTATGAAAGGTATCAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

582

Amino Acids

64.79

Weight (kDa)

6.0

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 75 - 577 1.6e-42 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 406
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 2 cut(s) 217, 788
AccB7I CCANNNNNTGG 1 cut(s) 683
AccII CGCG 1 cut(s) 1122
AciI CCGC 4 cut(s) 351, 365, 1648, 1707
AclWI GGATC 2 cut(s) 194, 1304
AcsI RAATTY 4 cut(s) 545, 687, 739, 1421
AcuI CTGAAG 4 cut(s) 830, 881, 1359, 1491
AfaI GTAC 4 cut(s) 219, 807, 844, 1523
AfiI CCNNNNNNNGG 4 cut(s) 572, 683, 1439, 1442
AflIII ACRYGT 2 cut(s) 1120, 1696
AgsI TTSAA 9 cut(s) 296, 308, 739, 746, 971, 1128, 1297, 1426, 1689
AjnI CCWGG 1 cut(s) 220
AjuI GAANNNNNNNTTGG 4 cut(s) 559, 591, 1213, 1245
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
Alw21I GWGCWC 1 cut(s) 1548
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 194, 1304
AlwNI CAGNNNCTG 1 cut(s) 274
AoxI GGCC 2 cut(s) 1162, 1283
ApeKI GCWGC 9 cut(s) 165, 383, 503, 658, 875, 903, 923, 962, 1089
ApoI RAATTY 4 cut(s) 545, 687, 739, 1421
Asp700I GAANNNNTTC 5 cut(s) 513, 718, 750, 1500, 1698
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 4 cut(s) 674, 1162, 1189, 1284
AsuC2I CCSGG 1 cut(s) 1334
AsuHPI GGTGA 4 cut(s) 209, 512, 762, 1103
AsuNHI GCTAGC 1 cut(s) 1715
AvaII GGWCC 2 cut(s) 674, 1189
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanI GGYRCC 2 cut(s) 217, 788
BarI GAAGNNNNNNTAC 2 cut(s) 90, 122
BbsI GAAGAC 3 cut(s) 98, 402, 818
Bbv12I GWGCWC 1 cut(s) 1548
BbvI GCAGC 9 cut(s) 177, 370, 515, 670, 862, 910, 915, 974, 1076
BccI CCATC 3 cut(s) 648, 677, 776
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 1 cut(s) 222
BciVI GTATCC 1 cut(s) 710
BclI TGATCA 1 cut(s) 1624
BcnI CCSGG 1 cut(s) 1334
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 5 cut(s) 279, 1028, 1340, 1716, 1747
BfmI CTRYAG 6 cut(s) 96, 579, 901, 924, 1157, 1464
BfuI GTATCC 1 cut(s) 710
BlpI GCTNAGC 2 cut(s) 161, 879
Bme1390I CCNGG 2 cut(s) 222, 1334
Bme18I GGWCC 2 cut(s) 674, 1189
BmgT120I GGNCC 4 cut(s) 674, 1162, 1189, 1284
BmiI GGNNCC 3 cut(s) 219, 790, 1503
BmrFI CCNGG 2 cut(s) 222, 1334
BmsI GCATC 2 cut(s) 438, 490
BmtI GCTAGC 1 cut(s) 1719
BpiI GAAGAC 3 cut(s) 98, 402, 818
BpmI CTGGAG 1 cut(s) 1461
Bpu1102I GCTNAGC 2 cut(s) 161, 879
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 1334
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 6 cut(s) 221, 235, 437, 1040, 1280, 1333
BsaXI ACNNNNNCTCC 6 cut(s) 314, 344, 408, 438, 1134, 1164
Bsc4I CCNNNNNNNGG 4 cut(s) 572, 683, 1439, 1442
Bse1I ACTGG 2 cut(s) 773, 1444
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 6 cut(s) 221, 235, 437, 1040, 1280, 1333
BseGI GGATG 2 cut(s) 505, 845
BseLI CCNNNNNNNGG 4 cut(s) 572, 683, 1439, 1442
BseMII CTCAG 6 cut(s) 175, 318, 564, 870, 981, 1400
BseNI ACTGG 2 cut(s) 773, 1444
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 9 cut(s) 177, 370, 515, 670, 862, 910, 915, 974, 1076
BsgI GTGCAG 1 cut(s) 1699
Bsh1236I CGCG 1 cut(s) 1122
BshFI GGCC 2 cut(s) 1164, 1285
BshNI GGYRCC 2 cut(s) 217, 788
BsiHKAI GWGCWC 1 cut(s) 1548
BsiSI CCGG 1 cut(s) 1333
BslFI GGGAC 1 cut(s) 1350
BslI CCNNNNNNNGG 4 cut(s) 572, 683, 1439, 1442
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 1350
BsmI GAATGC 1 cut(s) 1107
BsnI GGCC 2 cut(s) 1164, 1285
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 1548
Bsp143I GATC 4 cut(s) 199, 481, 1309, 1624
Bsp1720I GCTNAGC 2 cut(s) 161, 879
Bsp19I CCATGG 1 cut(s) 1040
BspACI CCGC 4 cut(s) 351, 365, 1648, 1707
BspANI GGCC 2 cut(s) 1164, 1285
BspCNI CTCAG 6 cut(s) 174, 319, 563, 871, 982, 1399
BspFNI CGCG 1 cut(s) 1122
BspHI TCATGA 2 cut(s) 1203, 1621
BspLI GGNNCC 3 cut(s) 219, 790, 1503
BspMAI CTGCAG 2 cut(s) 905, 928
BspOI GCTAGC 1 cut(s) 1719
BspPI GGATC 2 cut(s) 194, 1304
BspQI GCTCTTC 1 cut(s) 1549
BspT107I GGYRCC 2 cut(s) 217, 788
BspTNI GGTCTC 1 cut(s) 128
BsrI ACTGG 2 cut(s) 773, 1444
BssECI CCNNGG 6 cut(s) 221, 235, 437, 1040, 1280, 1333
BssMI GATC 4 cut(s) 199, 481, 1309, 1624
BssT1I CCWWGG 2 cut(s) 235, 1040
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 4 cut(s) 174, 1010, 1526, 1579
Bst6I CTCTTC 3 cut(s) 415, 1549, 1642
BstDEI CTNAG 9 cut(s) 161, 327, 334, 550, 592, 847, 879, 990, 1386
BstDSI CCRYGG 2 cut(s) 437, 1040
BstF5I GGATG 2 cut(s) 505, 845
BstFNI CGCG 1 cut(s) 1122
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 4 cut(s) 202, 484, 1312, 1627
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 4 cut(s) 199, 481, 1309, 1624
BstMWI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1261, 1712
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 1700
BstSCI CCNGG 2 cut(s) 220, 1332
BstSFI CTRYAG 6 cut(s) 96, 579, 901, 924, 1157, 1464
BstUI CGCG 1 cut(s) 1122
BstV1I GCAGC 9 cut(s) 177, 370, 515, 670, 862, 910, 915, 974, 1076
BstV2I GAAGAC 3 cut(s) 98, 402, 818
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 2 cut(s) 1164, 1285
BtgI CCRYGG 2 cut(s) 437, 1040
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 2 cut(s) 505, 845
BtsI GCAGTG 3 cut(s) 898, 1392, 1687
BtsIMutI CAGTG 7 cut(s) 179, 780, 898, 987, 1392, 1575, 1687
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 2 cut(s) 1203, 1621
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 4 cut(s) 674, 1162, 1189, 1284
CseI GACGC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 218, 806, 843, 1522
CspCI CAANNNNNGTGG 2 cut(s) 1197, 1232
CviAII CATG 7 cut(s) 53, 1041, 1204, 1459, 1605, 1622, 1697
CviQI GTAC 4 cut(s) 218, 806, 843, 1522
DdeI CTNAG 9 cut(s) 161, 327, 334, 550, 592, 847, 879, 990, 1386
DpnI GATC 4 cut(s) 201, 483, 1311, 1626
DpnII GATC 4 cut(s) 199, 481, 1309, 1624
Eam1104I CTCTTC 3 cut(s) 415, 1549, 1642
EarI CTCTTC 3 cut(s) 415, 1549, 1642
Eco130I CCWWGG 2 cut(s) 235, 1040
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 1 cut(s) 732
Eco47I GGWCC 2 cut(s) 674, 1189
Eco57I CTGAAG 4 cut(s) 830, 881, 1359, 1491
EcoRI GAATTC 3 cut(s) 545, 687, 1421
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 235, 1040
ErhI CCWWGG 2 cut(s) 235, 1040
FaeI CATG 7 cut(s) 56, 1044, 1207, 1462, 1608, 1625, 1700
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 1 cut(s) 1350
FatI CATG 7 cut(s) 52, 1040, 1203, 1458, 1604, 1621, 1696
FauI CCCGC 1 cut(s) 1655
FbaI TGATCA 1 cut(s) 1624
FokI GGATG 2 cut(s) 512, 852
FspBI CTAG 5 cut(s) 279, 1028, 1340, 1716, 1747
FspI TGCGCA 1 cut(s) 406
GlaI GCGC 1 cut(s) 406
GsuI CTGGAG 1 cut(s) 1461
HaeIII GGCC 2 cut(s) 1164, 1285
HapII CCGG 1 cut(s) 1333
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 407
Hin1II CATG 7 cut(s) 56, 1044, 1207, 1462, 1608, 1625, 1700
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HpaII CCGG 1 cut(s) 1333
HphI GGTGA 4 cut(s) 209, 512, 762, 1103
Hpy166II GTNNAC 4 cut(s) 437, 806, 870, 1392
Hpy188I TCNGA 6 cut(s) 328, 553, 1378, 1510, 1666, 1672
Hpy188III TCNNGA 6 cut(s) 10, 81, 253, 1028, 1204, 1622
Hpy8I GTNNAC 4 cut(s) 437, 806, 870, 1392
HpyAV CCTTC 3 cut(s) 533, 1366, 1515
HpyCH4III ACNGT 4 cut(s) 174, 1010, 1526, 1579
HpyCH4IV ACGT 2 cut(s) 193, 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1261, 1712
HpyF3I CTNAG 9 cut(s) 161, 327, 334, 550, 592, 847, 879, 990, 1386
HpySE526I ACGT 2 cut(s) 193, 258
Hsp92II CATG 7 cut(s) 56, 1044, 1207, 1462, 1608, 1625, 1700
HspAI GCGC 1 cut(s) 405
KpnI GGTACC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 1624
Kzo9I GATC 4 cut(s) 199, 481, 1309, 1624
LguI GCTCTTC 1 cut(s) 1549
LmnI GCTCC 2 cut(s) 1543, 1595
Lsp1109I GCAGC 9 cut(s) 177, 370, 515, 670, 862, 910, 915, 974, 1076
LweI GCATC 2 cut(s) 438, 490
MaeI CTAG 5 cut(s) 279, 1028, 1340, 1716, 1747
MaeII ACGT 2 cut(s) 193, 258
MaeIII GTNAC 2 cut(s) 19, 1004
MalI GATC 4 cut(s) 201, 483, 1311, 1626
MboI GATC 4 cut(s) 199, 481, 1309, 1624
MfeI CAATTG 3 cut(s) 244, 795, 966
MhlI GDGCHC 1 cut(s) 1548
MluI ACGCGT 1 cut(s) 1120
MlyI GAGTC 5 cut(s) 137, 332, 442, 1266, 1628
MmeI TCCRAC 2 cut(s) 73, 590
MroXI GAANNNNTTC 5 cut(s) 513, 718, 750, 1500, 1698
MseI TTAA 4 cut(s) 60, 536, 1196, 1322
MslI CAYNNNNRTG 1 cut(s) 1039
MspA1I CMGCKG 1 cut(s) 1650
MspI CCGG 1 cut(s) 1333
MspR9I CCNGG 2 cut(s) 222, 1334
MunI CAATTG 3 cut(s) 244, 795, 966
Mva1269I GAATGC 1 cut(s) 1107
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 1122
MwoI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1261, 1712
NciI CCSGG 1 cut(s) 1334
NcoI CCATGG 1 cut(s) 1040
NdeII GATC 4 cut(s) 199, 481, 1309, 1624
NheI GCTAGC 1 cut(s) 1715
NlaIII CATG 7 cut(s) 56, 1044, 1207, 1462, 1608, 1625, 1700
NlaIV GGNNCC 3 cut(s) 219, 790, 1503
NmeAIII GCCGAG 1 cut(s) 1261
NmuCI GTSAC 2 cut(s) 19, 1004
NsbI TGCGCA 1 cut(s) 406
NspI RCATGY 1 cut(s) 1700
PagI TCATGA 2 cut(s) 1203, 1621
PciI ACATGT 1 cut(s) 1696
PciSI GCTCTTC 1 cut(s) 1549
PctI GAATGC 1 cut(s) 1107
PdmI GAANNNNTTC 5 cut(s) 513, 718, 750, 1500, 1698
PfeI GAWTC 7 cut(s) 347, 746, 998, 1031, 1409, 1601, 1667
PflMI CCANNNNNTGG 1 cut(s) 683
PleI GAGTC 5 cut(s) 137, 331, 441, 1265, 1628
PpsI GAGTC 5 cut(s) 137, 331, 441, 1265, 1628
PscI ACATGT 1 cut(s) 1696
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 3 cut(s) 219, 790, 1503
PspPI GGNCC 4 cut(s) 674, 1162, 1189, 1284
PstI CTGCAG 2 cut(s) 905, 928
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 4 cut(s) 219, 807, 844, 1523
RsaNI GTAC 4 cut(s) 218, 806, 843, 1522
RseI CAYNNNNRTG 1 cut(s) 1039
SapI GCTCTTC 1 cut(s) 1549
SaqAI TTAA 4 cut(s) 60, 536, 1196, 1322
Sau3AI GATC 4 cut(s) 199, 481, 1309, 1624
Sau96I GGNCC 4 cut(s) 674, 1162, 1189, 1284
SchI GAGTC 5 cut(s) 137, 332, 442, 1266, 1628
ScrFI CCNGG 2 cut(s) 222, 1334
SduI GDGCHC 1 cut(s) 1548
SfaNI GCATC 2 cut(s) 438, 490
SfcI CTRYAG 6 cut(s) 96, 579, 901, 924, 1157, 1464
SinI GGWCC 2 cut(s) 674, 1189
SmiMI CAYNNNNRTG 1 cut(s) 1039
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SsiI CCGC 4 cut(s) 351, 365, 1648, 1707
SspI AATATT 2 cut(s) 935, 1327
SspMI CTAG 5 cut(s) 279, 1028, 1340, 1716, 1747
StyD4I CCNGG 2 cut(s) 220, 1332
StyI CCWWGG 2 cut(s) 235, 1040
TaaI ACNGT 4 cut(s) 174, 1010, 1526, 1579
TaiI ACGT 2 cut(s) 196, 261
TaqI TCGA 3 cut(s) 9, 712, 1046
TatI WGTACW 1 cut(s) 805
TauI GCSGC 2 cut(s) 367, 1709
TfiI GAWTC 7 cut(s) 347, 746, 998, 1031, 1409, 1601, 1667
Tru1I TTAA 4 cut(s) 60, 536, 1196, 1322
Tru9I TTAA 4 cut(s) 60, 536, 1196, 1322
TscAI CASTG 7 cut(s) 179, 780, 905, 994, 1399, 1582, 1687
TseFI GTSAC 2 cut(s) 19, 1004
TseI GCWGC 9 cut(s) 165, 383, 503, 658, 875, 903, 923, 962, 1089
Tsp45I GTSAC 2 cut(s) 19, 1004
TspDTI ATGAA 7 cut(s) 17, 276, 711, 1013, 1192, 1593, 1746
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 7 cut(s) 179, 780, 905, 994, 1399, 1582, 1687
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 674, 1189
XapI RAATTY 4 cut(s) 545, 687, 739, 1421
XbaI TCTAGA 1 cut(s) 1027
XceI RCATGY 1 cut(s) 1700
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 211
XmnI GAANNNNTTC 5 cut(s) 513, 718, 750, 1500, 1698
XspI CTAG 5 cut(s) 279, 1028, 1340, 1716, 1747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.