Rw2G021560

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010504
Physical Location & Seq
Reverse (-)
29932943 .. 29938098
5156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021560.1

Sequence Viewer

Length: 1461 bp
ATGAAGTTCGAGAGAGAGGTCACTCTAAATTCATTGGCTCGCCAATTCCAACATGGGTTTAAGACGCTTTCTCGTTCTTCTGGACTTCACATATCTGTGGCTGGTCTTCTGGGTATTGCTGGCCTCGTTTTGGCTCTCAAAGACTCCAAATTGGGCAAGCTCAGCAGCTTACAGTGGCTACCCAAGAACGAACGTGGTGATCCCAACTTGCATTTGGTACCTGGGTTGCAAAACCTTGGGAACAATTGTTTCCTGAACGTGATTTTACAGGCTCTGGCTAGTTGTTCATACTTTCAACCTTTTCTTCAAAAGGTTTTAGAGGAGTCTGAGTTCTTAGCAGTTGAGGATTCCGCAGATTGCGTGCCGCTTACAATATCTTTAGCTGCATTGTTAGAAGACCTATGCGCAGGTGGTGGAGGAAGAGTTGTATTGAGTCCACGGCCAGTGATGCTTGCCATAACACATTACATTGAGAACTTTGATCTAACAAGCGAGCAGGATGCAGCAGAAGCATTTCTTCACCTTCTGTCCTCTTTAAGAGAAGAATTCTCAGATTTTTATTGTCCAACTCAAGGTTCTATAGTGGAAGTCTTAGCCTCTAATGGTAGGATTATTACTCCAAAGAGAATGGAGAGCCAAAGTGAGCAGGAAAGATGGCAGCAACGCTTCTTTGGACCATTTGATGGAATTCTTGGCAGTATCCTAACCTGTCGAAGTTGTTCATCACAGATATCATTGAATTTTGAATCTTTTCACAGCTTGCCTCTTTCACCAGTACTTGATGGTGGTGCCACAATTATGGCTGGATGTACCTTAGAGGATTGCCTGAAGCAGTTTACTACTGCTGAGCAAGTTGAGAATTACCACTGCAGCCATTGTTGGCATATTGCTGCAGTCAAATATTTATTATCTATGGGAGGAAATGAGGTAAATCTATATTTTAATAAATCACTACCATGGTCGAATAGATTTTCACGCACTCTAAAGCAAATAAGTATTGCTCGCTGCCCAAAGATTTTGTGCATTCACCTAAAACGCGTTTCAATCAATGCATTTGGAGAACTTGTAAAACTACAGGGCCATATTTATTTTCCATTGGTTTTGGACCTTTTAAGTTTCATGACAAGTGGGGTGGGAATAAAGCATTGGGAGAAAAACCGGAAAACAGGGCGAGTCCGGCTACAAAGAGAAAACCCTCGGCCCAATCATTTCAATTTAGAGTATACTTGTTTGTACCGTCTTGTTGCTGTTGTGGAGCACTTTGGAAGAGCTGGAAGTGGGCATTACACTGTTTATAGAAGTATGGGAGTTGATTCATGTATGGAACAACCTCATGATCAATTTGACTCTTCTACTGCCCGCTGGTTTTCTATTTCAGATTCAGAGGTGTGCAGTGTTTCAAAGGAACATGTTCTTGCTGCAGAAGCTAGCTTGCTTTTCTATGAAAGGTATCAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

486

Amino Acids

54.4

Weight (kDa)

6.63

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 75 - 481 1.2e-42 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc16I TGCGCA 1 cut(s) 406
Acc36I ACCTGC 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 2 cut(s) 217, 788
AccB7I CCANNNNNTGG 1 cut(s) 683
AccI GTMKAC 1 cut(s) 1223
AccII CGCG 1 cut(s) 1038
AciI CCGC 3 cut(s) 351, 365, 1360
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 4 cut(s) 28, 545, 687, 739
AcuI CTGAAG 1 cut(s) 848
AfaI GTAC 4 cut(s) 219, 777, 811, 1235
AfiI CCNNNNNNNGG 3 cut(s) 130, 572, 683
AflIII ACRYGT 2 cut(s) 1036, 1408
AgsI TTSAA 7 cut(s) 296, 308, 739, 746, 1044, 1213, 1401
AjnI CCWGG 1 cut(s) 220
AjuI GAANNNNNNNTTGG 4 cut(s) 559, 591, 1129, 1161
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
AluBI AGCT 7 cut(s) 160, 168, 383, 759, 1271, 1427, 1431
AluI AGCT 7 cut(s) 160, 168, 383, 759, 1271, 1427, 1431
Alw21I GWGCWC 1 cut(s) 1260
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 274
AoxI GGCC 4 cut(s) 121, 440, 1078, 1199
ApeKI GCWGC 8 cut(s) 165, 383, 503, 658, 870, 890, 1005, 1418
ApoI RAATTY 4 cut(s) 28, 545, 687, 739
Asp700I GAANNNNTTC 4 cut(s) 513, 718, 750, 1410
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 4 cut(s) 674, 1078, 1105, 1200
AsuHPI GGTGA 4 cut(s) 209, 512, 762, 1019
AsuNHI GCTAGC 1 cut(s) 1427
AvaII GGWCC 2 cut(s) 674, 1105
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanI GGYRCC 2 cut(s) 217, 788
BbsI GAAGAC 2 cut(s) 98, 402
Bbv12I GWGCWC 1 cut(s) 1260
BbvI GCAGC 8 cut(s) 177, 370, 515, 670, 877, 882, 992, 1405
BccI CCATC 3 cut(s) 648, 677, 776
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 1 cut(s) 222
BciVI GTATCC 1 cut(s) 710
BclI TGATCA 1 cut(s) 1336
BfaI CTAG 3 cut(s) 279, 1428, 1459
BfmI CTRYAG 5 cut(s) 579, 868, 891, 1073, 1419
BfuAI ACCTGC 1 cut(s) 398
BfuI GTATCC 1 cut(s) 710
BisI GCNGC 9 cut(s) 166, 365, 384, 504, 659, 871, 891, 1006, 1419
BlpI GCTNAGC 2 cut(s) 161, 846
BlsI GCNGC 9 cut(s) 167, 366, 385, 505, 660, 872, 892, 1007, 1420
BmcAI AGTACT 1 cut(s) 777
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 2 cut(s) 674, 1105
BmgT120I GGNCC 4 cut(s) 674, 1078, 1105, 1200
BmiI GGNNCC 2 cut(s) 219, 790
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 438, 490
BmtI GCTAGC 1 cut(s) 1431
BpiI GAAGAC 2 cut(s) 98, 402
Bpu1102I GCTNAGC 2 cut(s) 161, 846
BpuEI CTTGAG 1 cut(s) 555
BsaJI CCNNGG 5 cut(s) 221, 235, 437, 956, 1196
BsaWI WCCGGW 1 cut(s) 1158
BsaXI ACNNNNNCTCC 6 cut(s) 314, 344, 408, 438, 1050, 1080
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 572, 683
Bse1I ACTGG 2 cut(s) 443, 773
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 5 cut(s) 221, 235, 437, 956, 1196
BseGI GGATG 2 cut(s) 505, 812
BseLI CCNNNNNNNGG 3 cut(s) 130, 572, 683
BseMII CTCAG 4 cut(s) 175, 318, 564, 837
BseNI ACTGG 2 cut(s) 443, 773
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 8 cut(s) 177, 370, 515, 670, 877, 882, 992, 1405
BsgI GTGCAG 1 cut(s) 1411
Bsh1236I CGCG 1 cut(s) 1038
BshFI GGCC 4 cut(s) 123, 442, 1080, 1201
BshNI GGYRCC 2 cut(s) 217, 788
BsiHKAI GWGCWC 1 cut(s) 1260
BsiSI CCGG 2 cut(s) 1159, 1177
BslI CCNNNNNNNGG 3 cut(s) 130, 572, 683
BsmI GAATGC 1 cut(s) 1023
BsnI GGCC 4 cut(s) 123, 442, 1080, 1201
Bsp1286I GDGCHC 1 cut(s) 1260
Bsp143I GATC 3 cut(s) 199, 481, 1336
Bsp1720I GCTNAGC 2 cut(s) 161, 846
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 3 cut(s) 351, 365, 1360
BspANI GGCC 4 cut(s) 123, 442, 1080, 1201
BspCNI CTCAG 4 cut(s) 174, 319, 563, 838
BspFNI CGCG 1 cut(s) 1038
BspHI TCATGA 2 cut(s) 1119, 1333
BspLI GGNNCC 2 cut(s) 219, 790
BspMAI CTGCAG 3 cut(s) 872, 895, 1423
BspMI ACCTGC 1 cut(s) 398
BspOI GCTAGC 1 cut(s) 1431
BspPI GGATC 1 cut(s) 194
BspQI GCTCTTC 1 cut(s) 1261
BspT107I GGYRCC 2 cut(s) 217, 788
BsrI ACTGG 2 cut(s) 443, 773
BssECI CCNNGG 5 cut(s) 221, 235, 437, 956, 1196
BssMI GATC 3 cut(s) 199, 481, 1336
BssNAI GTATAC 1 cut(s) 1224
BssT1I CCWWGG 2 cut(s) 235, 956
Bst1107I GTATAC 1 cut(s) 1224
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 3 cut(s) 174, 1238, 1291
Bst6I CTCTTC 3 cut(s) 415, 1261, 1354
BstDEI CTNAG 7 cut(s) 161, 327, 334, 550, 592, 814, 846
BstDSI CCRYGG 2 cut(s) 437, 956
BstF5I GGATG 2 cut(s) 505, 812
BstFNI CGCG 1 cut(s) 1038
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 3 cut(s) 202, 484, 1339
BstMBI GATC 3 cut(s) 199, 481, 1336
BstMWI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1177, 1424
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 1412
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 5 cut(s) 579, 868, 891, 1073, 1419
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 8 cut(s) 177, 370, 515, 670, 877, 882, 992, 1405
BstV2I GAAGAC 2 cut(s) 98, 402
BstXI CCANNNNNNTGG 1 cut(s) 799
BstZ17I GTATAC 1 cut(s) 1224
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 4 cut(s) 123, 442, 1080, 1201
BtgI CCRYGG 2 cut(s) 437, 956
BtsCI GGATG 2 cut(s) 505, 812
BtsI GCAGTG 2 cut(s) 865, 1399
BtsIMutI CAGTG 5 cut(s) 179, 450, 865, 1287, 1399
BveI ACCTGC 1 cut(s) 398
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 2 cut(s) 1119, 1333
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 4 cut(s) 674, 1078, 1105, 1200
CseI GACGC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 218, 776, 810, 1234
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
CviAII CATG 6 cut(s) 53, 957, 1120, 1317, 1334, 1409
CviQI GTAC 4 cut(s) 218, 776, 810, 1234
DdeI CTNAG 7 cut(s) 161, 327, 334, 550, 592, 814, 846
DpnI GATC 3 cut(s) 201, 483, 1338
DpnII GATC 3 cut(s) 199, 481, 1336
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 3 cut(s) 415, 1261, 1354
EarI CTCTTC 3 cut(s) 415, 1261, 1354
Eco130I CCWWGG 2 cut(s) 235, 956
Eco32I GATATC 1 cut(s) 732
Eco47I GGWCC 2 cut(s) 674, 1105
Eco57I CTGAAG 1 cut(s) 848
EcoRI GAATTC 2 cut(s) 545, 687
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 235, 956
EcoT22I ATGCAT 1 cut(s) 1054
ErhI CCWWGG 2 cut(s) 235, 956
FaeI CATG 6 cut(s) 56, 960, 1123, 1320, 1337, 1412
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FatI CATG 6 cut(s) 52, 956, 1119, 1316, 1333, 1408
FauI CCCGC 1 cut(s) 1367
FbaI TGATCA 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 1223
Fnu4HI GCNGC 9 cut(s) 166, 365, 384, 504, 659, 871, 891, 1006, 1419
FokI GGATG 2 cut(s) 512, 819
Fsp4HI GCNGC 9 cut(s) 166, 365, 384, 504, 659, 871, 891, 1006, 1419
FspBI CTAG 3 cut(s) 279, 1428, 1459
FspI TGCGCA 1 cut(s) 406
GlaI GCGC 1 cut(s) 406
GluI GCNGC 9 cut(s) 166, 365, 384, 504, 659, 871, 891, 1006, 1419
HaeIII GGCC 4 cut(s) 123, 442, 1080, 1201
HapII CCGG 2 cut(s) 1159, 1177
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 407
Hin1II CATG 6 cut(s) 56, 960, 1123, 1320, 1337, 1412
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HinfI GANTC 9 cut(s) 143, 323, 347, 433, 746, 1173, 1313, 1346, 1379
HpaII CCGG 2 cut(s) 1159, 1177
HphI GGTGA 4 cut(s) 209, 512, 762, 1019
Hpy166II GTNNAC 3 cut(s) 437, 837, 1224
Hpy188I TCNGA 4 cut(s) 328, 553, 1378, 1384
Hpy188III TCNNGA 5 cut(s) 10, 81, 253, 1120, 1334
Hpy8I GTNNAC 3 cut(s) 437, 837, 1224
HpyAV CCTTC 1 cut(s) 533
HpyCH4III ACNGT 3 cut(s) 174, 1238, 1291
HpyCH4IV ACGT 2 cut(s) 193, 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1177, 1424
HpyF3I CTNAG 7 cut(s) 161, 327, 334, 550, 592, 814, 846
HpySE526I ACGT 2 cut(s) 193, 258
Hsp92II CATG 6 cut(s) 56, 960, 1123, 1320, 1337, 1412
HspAI GCGC 1 cut(s) 405
KpnI GGTACC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 1336
Kzo9I GATC 3 cut(s) 199, 481, 1336
LguI GCTCTTC 1 cut(s) 1261
LmnI GCTCC 1 cut(s) 1255
Lsp1109I GCAGC 8 cut(s) 177, 370, 515, 670, 877, 882, 992, 1405
LweI GCATC 2 cut(s) 438, 490
MaeI CTAG 3 cut(s) 279, 1428, 1459
MaeII ACGT 2 cut(s) 193, 258
MaeIII GTNAC 1 cut(s) 19
MalI GATC 3 cut(s) 201, 483, 1338
MboI GATC 3 cut(s) 199, 481, 1336
MboII GAAGA 9 cut(s) 69, 98, 296, 407, 432, 509, 554, 1278, 1341
MfeI CAATTG 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 1260
MluI ACGCGT 1 cut(s) 1036
MlyI GAGTC 5 cut(s) 137, 332, 442, 1182, 1340
MmeI TCCRAC 2 cut(s) 73, 590
Mph1103I ATGCAT 1 cut(s) 1054
MroXI GAANNNNTTC 4 cut(s) 513, 718, 750, 1410
MseI TTAA 4 cut(s) 60, 536, 942, 1112
MslI CAYNNNNRTG 3 cut(s) 95, 797, 955
MspA1I CMGCKG 1 cut(s) 1362
MspI CCGG 2 cut(s) 1159, 1177
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 1038
MwoI GCNNNNNNNGC 5 cut(s) 162, 448, 509, 1177, 1424
NcoI CCATGG 1 cut(s) 956
NdeII GATC 3 cut(s) 199, 481, 1336
NheI GCTAGC 1 cut(s) 1427
NlaIII CATG 6 cut(s) 56, 960, 1123, 1320, 1337, 1412
NlaIV GGNNCC 2 cut(s) 219, 790
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 1 cut(s) 19
NsbI TGCGCA 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 1 cut(s) 1412
PagI TCATGA 2 cut(s) 1119, 1333
PaqCI CACCTGC 1 cut(s) 398
PciI ACATGT 1 cut(s) 1408
PciSI GCTCTTC 1 cut(s) 1261
PctI GAATGC 1 cut(s) 1023
PdmI GAANNNNTTC 4 cut(s) 513, 718, 750, 1410
PfeI GAWTC 4 cut(s) 347, 746, 1313, 1379
PflMI CCANNNNNTGG 1 cut(s) 683
PkrI GCNGC 9 cut(s) 167, 366, 385, 505, 660, 872, 892, 1007, 1420
PleI GAGTC 5 cut(s) 137, 331, 441, 1181, 1340
PpsI GAGTC 5 cut(s) 137, 331, 441, 1181, 1340
PscI ACATGT 1 cut(s) 1408
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 219, 790
PspPI GGNCC 4 cut(s) 674, 1078, 1105, 1200
PstI CTGCAG 3 cut(s) 872, 895, 1423
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 4 cut(s) 219, 777, 811, 1235
RsaNI GTAC 4 cut(s) 218, 776, 810, 1234
RseI CAYNNNNRTG 3 cut(s) 95, 797, 955
SapI GCTCTTC 1 cut(s) 1261
SaqAI TTAA 4 cut(s) 60, 536, 942, 1112
SatI GCNGC 9 cut(s) 166, 365, 384, 504, 659, 871, 891, 1006, 1419
Sau3AI GATC 3 cut(s) 199, 481, 1336
Sau96I GGNCC 4 cut(s) 674, 1078, 1105, 1200
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 5 cut(s) 137, 332, 442, 1182, 1340
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 1260
SfaNI GCATC 2 cut(s) 438, 490
SfcI CTRYAG 5 cut(s) 579, 868, 891, 1073, 1419
SinI GGWCC 2 cut(s) 674, 1105
SmiMI CAYNNNNRTG 3 cut(s) 95, 797, 955
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SsiI CCGC 3 cut(s) 351, 365, 1360
SspI AATATT 1 cut(s) 902
SspMI CTAG 3 cut(s) 279, 1428, 1459
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 2 cut(s) 235, 956
TaaI ACNGT 3 cut(s) 174, 1238, 1291
TaiI ACGT 2 cut(s) 196, 261
TaqI TCGA 3 cut(s) 9, 712, 962
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 4 cut(s) 347, 746, 1313, 1379
Tru1I TTAA 4 cut(s) 60, 536, 942, 1112
Tru9I TTAA 4 cut(s) 60, 536, 942, 1112
TscAI CASTG 5 cut(s) 179, 450, 872, 1294, 1399
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 8 cut(s) 165, 383, 503, 658, 870, 890, 1005, 1418
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 7 cut(s) 17, 21, 276, 711, 1108, 1305, 1458
TspRI CASTG 5 cut(s) 179, 450, 872, 1294, 1399
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 674, 1105
XapI RAATTY 4 cut(s) 28, 545, 687, 739
XceI RCATGY 1 cut(s) 1412
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 211
XmiI GTMKAC 1 cut(s) 1223
XmnI GAANNNNTTC 4 cut(s) 513, 718, 750, 1410
XspI CTAG 3 cut(s) 279, 1428, 1459
ZrmI AGTACT 1 cut(s) 777
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.