MD01G1048500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009715
Physical Location & Seq
Reverse (-)
15155617 .. 15158900
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1048500.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGGTCATGCAGACGGTACATCTGAGCCTCGGTCATCCAAGGATCGGTTACTTCCTTATGCTGAATCATCCGAGTTAGATGATGTTGACACTGCAGACTGTTCTGAGCAAATTCTTCACACGGCCTCCTTCGAAGAGCTTGCAAAGAATATCGTTAAGTATGACACTGTTATATGGCTTTCTATATCGCTGTTGCTGGTTTTAGCTTGGGGAGTTGGCATCATCATGCTTTTATATCTGCCCTTTAAGAGATATGTTCTTCAGAAGGATGTTTCCTCACGAAAACTATATGTTACGCCAAGTGAAGTAGTTTACAAGGTTTCGAGGCCATCTTTCATACCCTTCTGGGGCATCACTACAATTGAGAAGCATGTACCCCTTTCCCTGGTGATTGACATCATTATAGAACAAGGTTGCTTGCAGTCAAGGTATGGAATTCATACCTTTAGAATTGAAAGTATAGCATGTGGAAAAGCCGCAGCTGTAGATGAACTACAGGTTCAAGGGGTTTCTAACCCTAGTGGTTTGAGGAAGGTAATCATAACAGAAGCTTCAAAAGCCATACGAGAAAGTGGTGTAAGTTGGAAGCCTACTGCCACCACTGCTGAAGGGGAAAGCATGGCTCGTACTGGATCATTGACCGAGGGACCTCTTGTTTTGAGATCGCCATCTAAAAGCTTAAAGATGTCAGCATCACCACGCTATGCTTCAATAGAACGCAGAGGCATAGTACCTGGGGATTTACTACTTAATAAACTTGACGAAGTCAATAAATCTGTGAAGAGAATCGAGTTGTTAATTGAGAAGTCCCGTGCACACCCCGAGTCCTCAACTGAAATGAAGTCAGATGTACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.5

Weight (kDa)

7.7

Isoelectric Point (pI)

60.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 89 - 188 7e-47 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 477
AclWI GGATC 2 cut(s) 51, 640
AcsI RAATTY 2 cut(s) 111, 435
AcuI CTGAAG 2 cut(s) 245, 627
AfaI GTAC 5 cut(s) 19, 375, 628, 732, 852
AfiI CCNNNNNNNGG 3 cut(s) 45, 347, 385
AgsI TTSAA 4 cut(s) 455, 503, 555, 711
AjnI CCWGG 2 cut(s) 384, 733
AluBI AGCT 5 cut(s) 139, 206, 482, 551, 678
AluI AGCT 5 cut(s) 139, 206, 482, 551, 678
Alw21I GWGCWC 1 cut(s) 817
Alw44I GTGCAC 1 cut(s) 813
AlwI GGATC 2 cut(s) 51, 640
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 2 cut(s) 123, 326
ApaLI GTGCAC 1 cut(s) 813
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 111, 435
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 2 cut(s) 400, 687
AsuII TTCGAA 1 cut(s) 132
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 1 cut(s) 491
BccI CCATC 2 cut(s) 337, 676
BceAI ACGGC 1 cut(s) 138
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 2 cut(s) 386, 735
BfaI CTAG 2 cut(s) 519, 856
BfmI CTRYAG 3 cut(s) 93, 483, 494
BisI GCNGC 2 cut(s) 477, 480
BlsI GCNGC 2 cut(s) 478, 481
Bme1390I CCNGG 2 cut(s) 386, 735
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 2 cut(s) 386, 735
BmsI GCATC 3 cut(s) 228, 360, 701
Bpu14I TTCGAA 1 cut(s) 132
BsaBI GATNNNNATC 2 cut(s) 395, 667
BsaJI CCNNGG 5 cut(s) 29, 39, 384, 642, 734
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 347, 385
Bse1I ACTGG 1 cut(s) 634
Bse8I GATNNNNATC 2 cut(s) 395, 667
BseBI CCWGG 2 cut(s) 386, 735
BseDI CCNNGG 5 cut(s) 29, 39, 384, 642, 734
BseGI GGATG 3 cut(s) 35, 68, 274
BseJI GATNNNNATC 2 cut(s) 395, 667
BseLI CCNNNNNNNGG 3 cut(s) 45, 347, 385
BseMII CTCAG 2 cut(s) 15, 96
BseNI ACTGG 1 cut(s) 634
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 1 cut(s) 491
BshFI GGCC 2 cut(s) 125, 328
BsiHKAI GWGCWC 1 cut(s) 817
BsiHKCI CYCGRG 1 cut(s) 821
BslFI GGGAC 2 cut(s) 660, 793
BslI CCNNNNNNNGG 3 cut(s) 45, 347, 385
BsmFI GGGAC 2 cut(s) 660, 793
BsnI GGCC 2 cut(s) 125, 328
BsoBI CYCGRG 1 cut(s) 821
Bsp119I TTCGAA 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 817
Bsp143I GATC 3 cut(s) 43, 632, 662
BspACI CCGC 1 cut(s) 477
BspANI GGCC 2 cut(s) 125, 328
BspCNI CTCAG 2 cut(s) 16, 97
BspLI GGNNCC 1 cut(s) 648
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 2 cut(s) 51, 640
BspQI GCTCTTC 1 cut(s) 129
BspT104I TTCGAA 1 cut(s) 132
BsrI ACTGG 1 cut(s) 634
BssECI CCNNGG 5 cut(s) 29, 39, 384, 642, 734
BssMI GATC 3 cut(s) 43, 632, 662
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 386, 735
Bst4CI ACNGT 3 cut(s) 17, 101, 169
Bst6I CTCTTC 2 cut(s) 129, 776
BstBI TTCGAA 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 141, 419
BstDEI CTNAG 2 cut(s) 24, 105
BstF5I GGATG 3 cut(s) 35, 68, 274
BstKTI GATC 3 cut(s) 46, 635, 665
BstMBI GATC 3 cut(s) 43, 632, 662
BstMWI GCNNNNNNNGC 2 cut(s) 557, 602
BstNI CCWGG 2 cut(s) 386, 735
BstNSI RCATGY 2 cut(s) 374, 468
BstSCI CCNGG 2 cut(s) 384, 733
BstSFI CTRYAG 3 cut(s) 93, 483, 494
BstSLI GKGCMC 1 cut(s) 817
BstV1I GCAGC 1 cut(s) 491
BsuRI GGCC 2 cut(s) 125, 328
BtsCI GGATG 3 cut(s) 35, 68, 274
BtsI GCAGTG 2 cut(s) 90, 600
BtsIMutI CAGTG 3 cut(s) 90, 165, 600
Cac8I GCNNGC 2 cut(s) 141, 419
Cfr13I GGNCC 1 cut(s) 647
Csp6I GTAC 5 cut(s) 18, 374, 627, 731, 851
CviAII CATG 5 cut(s) 8, 226, 371, 465, 619
CviQI GTAC 5 cut(s) 18, 374, 627, 731, 851
DdeI CTNAG 2 cut(s) 24, 105
DpnI GATC 3 cut(s) 45, 634, 664
DpnII GATC 3 cut(s) 43, 632, 662
Eam1104I CTCTTC 2 cut(s) 129, 776
EarI CTCTTC 2 cut(s) 129, 776
Eco130I CCWWGG 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 2 cut(s) 245, 627
Eco88I CYCGRG 1 cut(s) 821
EcoO109I RGGNCCY 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 384, 733
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 5 cut(s) 11, 229, 374, 468, 622
FaqI GGGAC 2 cut(s) 660, 793
FatI CATG 5 cut(s) 7, 225, 370, 464, 618
Fnu4HI GCNGC 2 cut(s) 477, 480
FokI GGATG 3 cut(s) 22, 55, 281
Fsp4HI GCNGC 2 cut(s) 477, 480
FspBI CTAG 2 cut(s) 519, 856
GluI GCNGC 2 cut(s) 477, 480
HaeIII GGCC 2 cut(s) 125, 328
Hin1II CATG 5 cut(s) 11, 229, 374, 468, 622
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 2 cut(s) 549, 676
HinfI GANTC 3 cut(s) 65, 786, 824
HphI GGTGA 2 cut(s) 400, 687
Hpy166II GTNNAC 3 cut(s) 88, 313, 815
Hpy188I TCNGA 5 cut(s) 25, 73, 106, 264, 847
Hpy188III TCNNGA 1 cut(s) 279
Hpy8I GTNNAC 3 cut(s) 88, 313, 815
HpyAV CCTTC 5 cut(s) 139, 259, 352, 526, 602
HpyCH4III ACNGT 3 cut(s) 17, 101, 169
HpyCH4V TGCA 5 cut(s) 11, 95, 143, 421, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 602
HpyF3I CTNAG 2 cut(s) 24, 105
Hsp92II CATG 5 cut(s) 11, 229, 374, 468, 622
Kzo9I GATC 3 cut(s) 43, 632, 662
LguI GCTCTTC 1 cut(s) 129
LpnPI CCDG 8 cut(s) 182, 331, 371, 398, 482, 615, 720, 747
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 3 cut(s) 228, 360, 701
MaeI CTAG 2 cut(s) 519, 856
MaeIII GTNAC 2 cut(s) 48, 292
MalI GATC 3 cut(s) 45, 634, 664
MboI GATC 3 cut(s) 43, 632, 662
MboII GAAGA 4 cut(s) 107, 146, 251, 793
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 817
MluCI AATT 5 cut(s) 111, 360, 435, 450, 798
MlyI GAGTC 1 cut(s) 833
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 9 cut(s) 39, 136, 286, 318, 522, 637, 660, 716, 838
MseI TTAA 5 cut(s) 156, 246, 680, 750, 797
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 482
MspR9I CCNGG 2 cut(s) 386, 735
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 2 cut(s) 386, 735
MwoI GCNNNNNNNGC 2 cut(s) 557, 602
NdeII GATC 3 cut(s) 43, 632, 662
NlaIII CATG 5 cut(s) 11, 229, 374, 468, 622
NlaIV GGNNCC 1 cut(s) 648
NspI RCATGY 2 cut(s) 374, 468
NspV TTCGAA 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 129
PfeI GAWTC 2 cut(s) 65, 786
PflFI GACNNNGTC 1 cut(s) 764
PkrI GCNGC 2 cut(s) 478, 481
PleI GAGTC 1 cut(s) 832
PpsI GAGTC 1 cut(s) 832
PpuMI RGGWCCY 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 384, 733
PspGI CCWGG 2 cut(s) 384, 733
PspN4I GGNNCC 1 cut(s) 648
PspPI GGNCC 1 cut(s) 647
PspPPI RGGWCCY 1 cut(s) 647
PstI CTGCAG 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 764
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 5 cut(s) 19, 375, 628, 732, 852
RsaNI GTAC 5 cut(s) 18, 374, 627, 731, 851
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 5 cut(s) 156, 246, 680, 750, 797
SatI GCNGC 2 cut(s) 477, 480
Sau3AI GATC 3 cut(s) 43, 632, 662
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 2 cut(s) 386, 735
SduI GDGCHC 1 cut(s) 817
SfaNI GCATC 3 cut(s) 228, 360, 701
SfcI CTRYAG 3 cut(s) 93, 483, 494
SfuI TTCGAA 1 cut(s) 132
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 224
Sse9I AATT 5 cut(s) 111, 360, 435, 450, 798
SsiI CCGC 1 cut(s) 477
SspMI CTAG 2 cut(s) 519, 856
StyD4I CCNGG 2 cut(s) 384, 733
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 17, 101, 169
TaqI TCGA 3 cut(s) 132, 323, 789
TaqII GACCGA 2 cut(s) 21, 656
TasI AATT 5 cut(s) 111, 360, 435, 450, 798
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 2 cut(s) 65, 786
Tru1I TTAA 5 cut(s) 156, 246, 680, 750, 797
Tru9I TTAA 5 cut(s) 156, 246, 680, 750, 797
TscAI CASTG 3 cut(s) 97, 172, 607
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 4 cut(s) 325, 428, 504, 854
TspRI CASTG 3 cut(s) 97, 172, 607
Tth111I GACNNNGTC 1 cut(s) 764
VneI GTGCAC 1 cut(s) 813
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 2 cut(s) 111, 435
XceI RCATGY 2 cut(s) 374, 468
XspI CTAG 2 cut(s) 519, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.