RLG00000028290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009715
Physical Location & Seq
Reverse (-)
21088603 .. 21090602
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028290

Sequence Viewer

Length: 900 bp
ATGTTGATGGGTCATACAGATGGTCTTTCTGAATCTCGGTCATCGAGGGATCAGCTACTTCCTTATGCTGAATCTGACTTAGGGGATGATGACACTGCAGACTGTTCTGAGCCAATACTGTACTCAGCCTCCTTTGAAGAGCTTGCCAAAAATATCGTTAAGTATGACACCGTTATATGGCTCTCAATATCATTGATGCTGGTTTTAGCTTGGGGAGTTGGCATCATCATGCTGTTATATCTACCCTTTAAGAGATATATTCTCCAGAAGGACATCTCCTCCCGCAAGCTATATGTTACAGCTAGTGAAATAGTCTACAAGGTGTCAAGGCCTTCTTATGTACCCTTTTTGGGCATTACTACAATTGAGAGGCATGTGCCCCTTTCCCTGGTGATTGACATCATTATTGAACAAGGTTGTTTGCAGTCAAAATATGGACTTCATACCTTTCGAATTGAAAGTATAGCACGTGGAAAAGCTGCAGCTGTTGATGAACTACAGGTTCAAGGGGTTTCTAACCCTAGTGATTTGAGGAAGGTCATCATAACAGAAGCTTCAAAAGTTATACAAGATAGCGGTATAAGTTGGAAGCCTACTACTGTCACTGCAGAAGGGGAAAGCATGGATCTATCTCGTACAAGTTGGAAGCCTACTACTGTCACTGCAGAAGGGGAAAGCATGGCTTGTACTCCATCCGTGACTGAAGCACCAATTCTGAGATCACCAGCTAAAAGCGTAAAGATGGCAGCTTCACCACGCTATGCTTCTATAGAACGCAGAAGCATACTACCTAGTGAATTGCTGCTTAATAGACTTGAAGAAGTCAATAAATCTGTAAAGAAAATCGAGTTTTTAATAGAGAAGTCACATACTCAACCTGAGTTAGAGCGGCTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

33.28

Weight (kDa)

5.73

Isoelectric Point (pI)

64.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 90 - 189 2.2e-46 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 889
AccI GTMKAC 1 cut(s) 315
AciI CCGC 3 cut(s) 283, 576, 889
AclWI GGATC 2 cut(s) 57, 633
AcuI CTGAAG 1 cut(s) 723
AcvI CACGTG 1 cut(s) 470
AfaI GTAC 4 cut(s) 122, 342, 637, 688
AfiI CCNNNNNNNGG 3 cut(s) 177, 350, 388
AgsI TTSAA 6 cut(s) 137, 410, 458, 506, 558, 818
AjnI CCWGG 1 cut(s) 387
AlwI GGATC 2 cut(s) 57, 633
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 4 cut(s) 479, 482, 746, 802
AsuHPI GGTGA 3 cut(s) 403, 714, 744
AsuII TTCGAA 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 381
BbrPI CACGTG 1 cut(s) 470
BbvI GCAGC 4 cut(s) 466, 494, 758, 789
BccI CCATC 3 cut(s) 14, 700, 736
BciT130I CCWGG 1 cut(s) 389
BfaI CTAG 3 cut(s) 303, 522, 792
BfmI CTRYAG 6 cut(s) 96, 480, 497, 606, 663, 768
BisI GCNGC 5 cut(s) 480, 483, 747, 803, 890
BlsI GCNGC 5 cut(s) 481, 484, 748, 804, 891
Bme1390I CCNGG 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 186, 231
BpmI CTGGAG 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 387
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 350, 388
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 91, 692
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BseMII CTCAG 4 cut(s) 99, 138, 707, 870
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 4 cut(s) 466, 494, 758, 789
BshFI GGCC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BsnI GGCC 1 cut(s) 331
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 3 cut(s) 49, 625, 719
BspACI CCGC 3 cut(s) 283, 576, 889
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 4 cut(s) 100, 137, 708, 871
BspMAI CTGCAG 4 cut(s) 100, 484, 610, 667
BspPI GGATC 2 cut(s) 57, 633
BspQI GCTCTTC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 889
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 3 cut(s) 49, 625, 719
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 5 cut(s) 104, 120, 172, 601, 658
Bst6I CTCTTC 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 144, 287
BstDEI CTNAG 5 cut(s) 79, 108, 124, 716, 879
BstF5I GGATG 2 cut(s) 91, 692
BstKTI GATC 3 cut(s) 52, 628, 722
BstMBI GATC 3 cut(s) 49, 625, 719
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 6 cut(s) 96, 480, 497, 606, 663, 768
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 4 cut(s) 466, 494, 758, 789
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 91, 692
BtsI GCAGTG 3 cut(s) 93, 603, 660
BtsIMutI CAGTG 3 cut(s) 93, 603, 660
Cac8I GCNNGC 2 cut(s) 144, 287
Csp6I GTAC 4 cut(s) 121, 341, 636, 687
CviAII CATG 4 cut(s) 229, 374, 622, 679
CviQI GTAC 4 cut(s) 121, 341, 636, 687
DdeI CTNAG 5 cut(s) 79, 108, 124, 716, 879
DpnI GATC 3 cut(s) 51, 627, 721
DpnII GATC 3 cut(s) 49, 625, 719
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 723
Eco72I CACGTG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 232, 377, 625, 682
FalI AAGNNNNNCTT 4 cut(s) 319, 351, 667, 699
FatI CATG 4 cut(s) 228, 373, 621, 678
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 480, 483, 747, 803, 890
FokI GGATG 2 cut(s) 98, 679
Fsp4HI GCNGC 5 cut(s) 480, 483, 747, 803, 890
FspBI CTAG 3 cut(s) 303, 522, 792
GluI GCNGC 5 cut(s) 480, 483, 747, 803, 890
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 331
Hin1II CATG 4 cut(s) 232, 377, 625, 682
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 2 cut(s) 32, 71
HphI GGTGA 3 cut(s) 403, 714, 744
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 31, 76, 109, 717
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 1 cut(s) 316
HpyAV CCTTC 5 cut(s) 262, 342, 529, 605, 662
HpyCH4III ACNGT 5 cut(s) 104, 120, 172, 601, 658
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 5 cut(s) 98, 424, 482, 608, 665
HpyF3I CTNAG 5 cut(s) 79, 108, 124, 716, 879
HpySE526I ACGT 1 cut(s) 469
Hsp92II CATG 4 cut(s) 232, 377, 625, 682
Kzo9I GATC 3 cut(s) 49, 625, 719
LguI GCTCTTC 1 cut(s) 132
LpnPI CCDG 7 cut(s) 185, 278, 374, 401, 485, 738, 891
Lsp1109I GCAGC 4 cut(s) 466, 494, 758, 789
LweI GCATC 2 cut(s) 186, 231
MaeI CTAG 3 cut(s) 303, 522, 792
MaeII ACGT 1 cut(s) 469
MaeIII GTNAC 5 cut(s) 295, 601, 658, 697, 864
MalI GATC 3 cut(s) 51, 627, 721
MbiI CCGCTC 1 cut(s) 889
MboI GATC 3 cut(s) 49, 625, 719
MboII GAAGA 2 cut(s) 149, 830
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 5 cut(s) 363, 453, 711, 797, 895
MmeI TCCRAC 2 cut(s) 566, 623
MnlI CCTC 5 cut(s) 39, 139, 289, 363, 525
MseI TTAA 4 cut(s) 159, 249, 807, 854
MslI CAYNNNNRTG 2 cut(s) 18, 227
MspA1I CMGCKG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 389
NdeII GATC 3 cut(s) 49, 625, 719
NlaIII CATG 4 cut(s) 232, 377, 625, 682
NmuCI GTSAC 4 cut(s) 601, 658, 697, 864
NspI RCATGY 1 cut(s) 377
NspV TTCGAA 1 cut(s) 451
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 2 cut(s) 32, 71
PkrI GCNGC 5 cut(s) 481, 484, 748, 804, 891
PmaCI CACGTG 1 cut(s) 470
PmlI CACGTG 1 cut(s) 470
Ppu21I YACGTR 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 387
PspCI CACGTG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 387
PstI CTGCAG 4 cut(s) 100, 484, 610, 667
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 4 cut(s) 122, 342, 637, 688
RsaNI GTAC 4 cut(s) 121, 341, 636, 687
RseI CAYNNNNRTG 2 cut(s) 18, 227
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 4 cut(s) 159, 249, 807, 854
SatI GCNGC 5 cut(s) 480, 483, 747, 803, 890
Sau3AI GATC 3 cut(s) 49, 625, 719
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 2 cut(s) 186, 231
SfcI CTRYAG 6 cut(s) 96, 480, 497, 606, 663, 768
SfuI TTCGAA 1 cut(s) 451
SmiMI CAYNNNNRTG 2 cut(s) 18, 227
Sse9I AATT 5 cut(s) 363, 453, 711, 797, 895
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 3 cut(s) 283, 576, 889
SspMI CTAG 3 cut(s) 303, 522, 792
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 5 cut(s) 104, 120, 172, 601, 658
TaiI ACGT 1 cut(s) 472
TaqI TCGA 3 cut(s) 44, 451, 846
TaqII GACCGA 1 cut(s) 27
TasI AATT 5 cut(s) 363, 453, 711, 797, 895
TatI WGTACW 2 cut(s) 120, 686
TauI GCSGC 1 cut(s) 892
TfiI GAWTC 2 cut(s) 32, 71
Tru1I TTAA 4 cut(s) 159, 249, 807, 854
Tru9I TTAA 4 cut(s) 159, 249, 807, 854
TscAI CASTG 3 cut(s) 100, 610, 667
TseFI GTSAC 4 cut(s) 601, 658, 697, 864
TseI GCWGC 4 cut(s) 479, 482, 746, 802
Tsp45I GTSAC 4 cut(s) 601, 658, 697, 864
TspDTI ATGAA 2 cut(s) 431, 507
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 3 cut(s) 100, 610, 667
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 3 cut(s) 303, 522, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.