Rw0G018100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00801
OG0009715
Physical Location & Seq
Reverse (-)
27119 .. 30510
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018100.1

Sequence Viewer

Length: 843 bp
ATGTTGATGGGTCATACAGATGGTCTTTCTGAATCTCGGTCATCGAGGGATCAGCTACTTCCTTATGCTGAATCTGACTTAGGGGATGATGACACTGCAGACTGTTCTGAGCCAATACTGTACTCAGCCTCCTTTGAAGAGCTTGCCAAAAATATTGTTAAGTATGACACCGTTATATGGCTCTCAATATCATTGATGCTTGTTTTAGCTTGGGGAGTTGGCATCATCATGCTGTTATATCTACCCTTTAAGAGATATATTCTCCAGAAGGACATCTCCTCCCGCAAGCTATATGTTACACCTAGTGAAATAGTCTACAAGGTGTCAAGGCCTTCTTATGTACCCTTTTTGGGCATCACTACAATTGAGAGGCATGTGCCCCTTTCCCTGGTGATTGACATCATTATTGAACAAGGTTGTTTGCAGTCAAAATATGGACTTCATACCTTTCGAATTGAAAGTATAGCATGTGGAAAAGCTGCAGCTGTTGATGAACTACAGGTTCAAGGGGTTTCAAACCCTAGTGATTTGAGGAAGGTCATCATAACAGAAGCTTCAAAAGTTATACAAGATAGCGGTATAAGTTGGAAGCCTACTACTGTCACTGCAGAAGGGGAAAGCATGGCTCGTACACCATCCGTGACTGAAGCACCAATTCTGAGATCACCAGCTAAAAGCGTAAAGATGGCAGCTTCACCACGCTATGCTTCAATAGAACGCAGAAGCATACTACCTAGTGAATTGCTGCTTAATAGACTTGAAGAAGTCAATAAATCTGTAAAGAAAATCGAGTTTTTAATAGAGAAGTCACATACTCAACCTGAGTTAGAGCGGCTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.23

Weight (kDa)

5.91

Isoelectric Point (pI)

67.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 90 - 189 9.7e-47 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 832
AccI GTMKAC 1 cut(s) 315
AciI CCGC 3 cut(s) 283, 576, 832
AclWI GGATC 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 666
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 3 cut(s) 122, 342, 631
AfiI CCNNNNNNNGG 3 cut(s) 177, 350, 388
AgsI TTSAA 8 cut(s) 137, 410, 458, 506, 516, 558, 711, 761
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 9 cut(s) 55, 142, 209, 289, 479, 485, 554, 671, 692
AluI AGCT 9 cut(s) 55, 142, 209, 289, 479, 485, 554, 671, 692
AlwI GGATC 1 cut(s) 57
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 4 cut(s) 479, 482, 689, 745
AsuHPI GGTGA 3 cut(s) 403, 657, 687
AsuII TTCGAA 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 381
BbvI GCAGC 4 cut(s) 466, 494, 701, 732
BccI CCATC 3 cut(s) 14, 643, 679
BciT130I CCWGG 1 cut(s) 389
BfaI CTAG 3 cut(s) 303, 522, 735
BfmI CTRYAG 4 cut(s) 96, 480, 497, 606
BisI GCNGC 5 cut(s) 480, 483, 690, 746, 833
BlsI GCNGC 5 cut(s) 481, 484, 691, 747, 834
Bme1390I CCNGG 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 3 cut(s) 186, 231, 363
BpmI CTGGAG 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 387
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 350, 388
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 91, 635
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BseMII CTCAG 4 cut(s) 99, 138, 650, 813
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 4 cut(s) 466, 494, 701, 732
BshFI GGCC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BsnI GGCC 1 cut(s) 331
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 49, 662
BspACI CCGC 3 cut(s) 283, 576, 832
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 4 cut(s) 100, 137, 651, 814
BspMAI CTGCAG 3 cut(s) 100, 484, 610
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 832
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 49, 662
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 4 cut(s) 104, 120, 172, 601
Bst6I CTCTTC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 144, 287
BstDEI CTNAG 5 cut(s) 79, 108, 124, 659, 822
BstF5I GGATG 2 cut(s) 91, 635
BstKTI GATC 2 cut(s) 52, 665
BstMBI GATC 2 cut(s) 49, 662
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 2 cut(s) 377, 471
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 4 cut(s) 96, 480, 497, 606
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 4 cut(s) 466, 494, 701, 732
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 91, 635
BtsI GCAGTG 2 cut(s) 93, 603
BtsIMutI CAGTG 2 cut(s) 93, 603
Cac8I GCNNGC 2 cut(s) 144, 287
Csp6I GTAC 3 cut(s) 121, 341, 630
CviAII CATG 4 cut(s) 229, 374, 468, 622
CviQI GTAC 3 cut(s) 121, 341, 630
DdeI CTNAG 5 cut(s) 79, 108, 124, 659, 822
DpnI GATC 2 cut(s) 51, 664
DpnII GATC 2 cut(s) 49, 662
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 666
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 232, 377, 471, 625
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FatI CATG 4 cut(s) 228, 373, 467, 621
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 480, 483, 690, 746, 833
FokI GGATG 2 cut(s) 98, 622
Fsp4HI GCNGC 5 cut(s) 480, 483, 690, 746, 833
FspBI CTAG 3 cut(s) 303, 522, 735
GluI GCNGC 5 cut(s) 480, 483, 690, 746, 833
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 331
Hin1II CATG 4 cut(s) 232, 377, 471, 625
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 2 cut(s) 32, 71
HphI GGTGA 3 cut(s) 403, 657, 687
Hpy166II GTNNAC 2 cut(s) 316, 632
Hpy188I TCNGA 4 cut(s) 31, 76, 109, 660
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 2 cut(s) 316, 632
HpyAV CCTTC 4 cut(s) 262, 342, 529, 605
HpyCH4III ACNGT 4 cut(s) 104, 120, 172, 601
HpyCH4V TGCA 4 cut(s) 98, 424, 482, 608
HpyF3I CTNAG 5 cut(s) 79, 108, 124, 659, 822
Hsp92II CATG 4 cut(s) 232, 377, 471, 625
Kzo9I GATC 2 cut(s) 49, 662
LguI GCTCTTC 1 cut(s) 132
LpnPI CCDG 6 cut(s) 278, 374, 401, 485, 681, 834
Lsp1109I GCAGC 4 cut(s) 466, 494, 701, 732
LweI GCATC 3 cut(s) 186, 231, 363
MaeI CTAG 3 cut(s) 303, 522, 735
MaeIII GTNAC 4 cut(s) 295, 601, 640, 807
MalI GATC 2 cut(s) 51, 664
MbiI CCGCTC 1 cut(s) 832
MboI GATC 2 cut(s) 49, 662
MboII GAAGA 2 cut(s) 149, 773
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 5 cut(s) 363, 453, 654, 740, 838
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 5 cut(s) 39, 139, 289, 363, 525
MseI TTAA 4 cut(s) 159, 249, 750, 797
MslI CAYNNNNRTG 2 cut(s) 18, 227
MspA1I CMGCKG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 389
NdeII GATC 2 cut(s) 49, 662
NlaIII CATG 4 cut(s) 232, 377, 471, 625
NmuCI GTSAC 3 cut(s) 601, 640, 807
NspI RCATGY 2 cut(s) 377, 471
NspV TTCGAA 1 cut(s) 451
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 2 cut(s) 32, 71
PkrI GCNGC 5 cut(s) 481, 484, 691, 747, 834
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PstI CTGCAG 3 cut(s) 100, 484, 610
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 3 cut(s) 122, 342, 631
RsaNI GTAC 3 cut(s) 121, 341, 630
RseI CAYNNNNRTG 2 cut(s) 18, 227
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 4 cut(s) 159, 249, 750, 797
SatI GCNGC 5 cut(s) 480, 483, 690, 746, 833
Sau3AI GATC 2 cut(s) 49, 662
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 3 cut(s) 186, 231, 363
SfcI CTRYAG 4 cut(s) 96, 480, 497, 606
SfuI TTCGAA 1 cut(s) 451
SmiMI CAYNNNNRTG 2 cut(s) 18, 227
Sse9I AATT 5 cut(s) 363, 453, 654, 740, 838
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 3 cut(s) 283, 576, 832
SspI AATATT 1 cut(s) 154
SspMI CTAG 3 cut(s) 303, 522, 735
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 4 cut(s) 104, 120, 172, 601
TaqI TCGA 3 cut(s) 44, 451, 789
TaqII GACCGA 1 cut(s) 27
TasI AATT 5 cut(s) 363, 453, 654, 740, 838
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 2 cut(s) 32, 71
Tru1I TTAA 4 cut(s) 159, 249, 750, 797
Tru9I TTAA 4 cut(s) 159, 249, 750, 797
TscAI CASTG 2 cut(s) 100, 610
TseFI GTSAC 3 cut(s) 601, 640, 807
TseI GCWGC 4 cut(s) 479, 482, 689, 745
Tsp45I GTSAC 3 cut(s) 601, 640, 807
TspDTI ATGAA 2 cut(s) 431, 507
TspGWI ACGGA 1 cut(s) 628
TspRI CASTG 2 cut(s) 100, 610
XceI RCATGY 2 cut(s) 377, 471
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 3 cut(s) 303, 522, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.