Rh1DG243300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0009715
Physical Location & Seq
Forward (+)
45890484 .. 45893769
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG243300.1

Sequence Viewer

Length: 609 bp
ATGTTGATGGGTCATACAGATGGTCTTTCTGAATCTCGGTCATCGAGGGATCAGCTACTTCCTTATGCTGAATCTGACTTAGGGGATGATGACACTGCAGACTGTTCTGAGCCAATACTGTACTCAGCCTCCTTTGAAGAGCTTGCCAAAAATATCGTTAAGTATGACACCGTTATATGGCTCTCAATATCATTGATGCTGGTTTTAGCTTGGGGAGTTGGCATCATCATGCTGTTATATCTACCCTTTAAAAGATATATTCTCCAGAAGGACATCTCCTCCCGCAAGCTATATGTTACACCTAGTGAAATAGTCTACAAGGTGTCAAGGCCTTCTTATGTACCCTTTTTGGGCATCACTACAATTGAGAGGCATGTGCCCCTTTCCCTGGTGATTGACATCATTATTGAACAAGGTTGTTTGCAGTCAAAATATGGACTTCATACCTTTCGAATTGAAAGTATAGCACGTGGAAAAGCTGCAGCTGTTGATGAACTACAGGTTCAAGGGGTTTCTAACCCTAGTGATTTGAGGAAGGTGAATAAAACTCTCTTATTTTTTTTTTTTTCGTTTGAATCAGAAAGCATGCTCATTAAAGAAAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.87

Weight (kDa)

5.25

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7642 PF24649 90 - 182 2.4e-44 Domain of unknown function (DUF7642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AciI CCGC 1 cut(s) 283
AclWI GGATC 1 cut(s) 57
AcsI RAATTY 1 cut(s) 601
AcvI CACGTG 1 cut(s) 470
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 2 cut(s) 122, 342
AfiI CCNNNNNNNGG 3 cut(s) 177, 350, 388
AgsI TTSAA 5 cut(s) 137, 410, 458, 506, 575
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 6 cut(s) 55, 142, 209, 289, 479, 485
AluI AGCT 6 cut(s) 55, 142, 209, 289, 479, 485
AlwI GGATC 1 cut(s) 57
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 479, 482
ApoI RAATTY 1 cut(s) 601
AsuHPI GGTGA 2 cut(s) 403, 550
AsuII TTCGAA 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 381
BbrPI CACGTG 1 cut(s) 470
BbvI GCAGC 2 cut(s) 466, 494
BccI CCATC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 389
BfaI CTAG 2 cut(s) 303, 522
BfmI CTRYAG 3 cut(s) 96, 480, 497
BisI GCNGC 2 cut(s) 480, 483
BlsI GCNGC 2 cut(s) 481, 484
Bme1390I CCNGG 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 3 cut(s) 186, 231, 363
BpmI CTGGAG 1 cut(s) 248
Bpu14I TTCGAA 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 387
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 350, 388
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 1 cut(s) 91
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BseMII CTCAG 2 cut(s) 99, 138
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 2 cut(s) 466, 494
BshFI GGCC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 177, 350, 388
BsnI GGCC 1 cut(s) 331
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 1 cut(s) 283
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 2 cut(s) 100, 137
BspMAI CTGCAG 2 cut(s) 100, 484
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 3 cut(s) 104, 120, 172
Bst6I CTCTTC 1 cut(s) 132
BstBAI YACGTR 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 3 cut(s) 144, 287, 587
BstDEI CTNAG 3 cut(s) 79, 108, 124
BstF5I GGATG 1 cut(s) 91
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 2 cut(s) 377, 589
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 3 cut(s) 96, 480, 497
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 466, 494
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 3 cut(s) 144, 287, 587
Csp6I GTAC 2 cut(s) 121, 341
CviAII CATG 3 cut(s) 229, 374, 586
CviQI GTAC 2 cut(s) 121, 341
DdeI CTNAG 3 cut(s) 79, 108, 124
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DraI TTTAAA 1 cut(s) 250
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 331
Eco72I CACGTG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 3 cut(s) 232, 377, 589
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FatI CATG 3 cut(s) 228, 373, 585
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 480, 483
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 2 cut(s) 480, 483
FspBI CTAG 2 cut(s) 303, 522
GluI GCNGC 2 cut(s) 480, 483
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 331
Hin1II CATG 3 cut(s) 232, 377, 589
HinfI GANTC 3 cut(s) 32, 71, 575
HphI GGTGA 2 cut(s) 403, 550
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 31, 76, 109, 580
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 1 cut(s) 316
HpyAV CCTTC 3 cut(s) 262, 342, 529
HpyCH4III ACNGT 3 cut(s) 104, 120, 172
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 3 cut(s) 98, 424, 482
HpyF3I CTNAG 3 cut(s) 79, 108, 124
HpySE526I ACGT 1 cut(s) 469
Hsp92II CATG 3 cut(s) 232, 377, 589
Kzo9I GATC 1 cut(s) 49
LguI GCTCTTC 1 cut(s) 132
LpnPI CCDG 5 cut(s) 185, 278, 374, 401, 485
Lsp1109I GCAGC 2 cut(s) 466, 494
LweI GCATC 3 cut(s) 186, 231, 363
MaeI CTAG 2 cut(s) 303, 522
MaeII ACGT 1 cut(s) 469
MaeIII GTNAC 1 cut(s) 295
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 1 cut(s) 149
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 3 cut(s) 363, 453, 601
MnlI CCTC 5 cut(s) 39, 139, 289, 363, 525
MseI TTAA 3 cut(s) 159, 249, 594
MslI CAYNNNNRTG 2 cut(s) 18, 227
MspA1I CMGCKG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 389
NdeII GATC 1 cut(s) 49
NlaIII CATG 3 cut(s) 232, 377, 589
NspI RCATGY 2 cut(s) 377, 589
NspV TTCGAA 1 cut(s) 451
PaeI GCATGC 1 cut(s) 589
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 3 cut(s) 32, 71, 575
PkrI GCNGC 2 cut(s) 481, 484
PmaCI CACGTG 1 cut(s) 470
PmlI CACGTG 1 cut(s) 470
Ppu21I YACGTR 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 387
PspCI CACGTG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 387
PstI CTGCAG 2 cut(s) 100, 484
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 2 cut(s) 122, 342
RsaNI GTAC 2 cut(s) 121, 341
RseI CAYNNNNRTG 2 cut(s) 18, 227
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 159, 249, 594
SatI GCNGC 2 cut(s) 480, 483
Sau3AI GATC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 3 cut(s) 186, 231, 363
SfcI CTRYAG 3 cut(s) 96, 480, 497
SfuI TTCGAA 1 cut(s) 451
SmiMI CAYNNNNRTG 2 cut(s) 18, 227
SphI GCATGC 1 cut(s) 589
Sse9I AATT 3 cut(s) 363, 453, 601
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 1 cut(s) 283
SspMI CTAG 2 cut(s) 303, 522
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 3 cut(s) 104, 120, 172
TaiI ACGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 44, 451
TaqII GACCGA 1 cut(s) 27
TasI AATT 3 cut(s) 363, 453, 601
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 3 cut(s) 32, 71, 575
Tru1I TTAA 3 cut(s) 159, 249, 594
Tru9I TTAA 3 cut(s) 159, 249, 594
TscAI CASTG 1 cut(s) 100
TseI GCWGC 2 cut(s) 479, 482
TspDTI ATGAA 2 cut(s) 431, 507
TspRI CASTG 1 cut(s) 100
XapI RAATTY 1 cut(s) 601
XceI RCATGY 2 cut(s) 377, 589
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 2 cut(s) 303, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.