MD01G1050900.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012773
Physical Location & Seq
Forward (+)
15427179 .. 15428215
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1050900.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGAAGAATCTGATATGTTGTGTTCTCTTTTCCATTTGTGTAGATTCCAGACAGTTGCCTATCTCCAACAAATCTGGTGGTTTGAAATCAATGGGGAGTTTGAGTTTCAATTTCCGTTAGGGAAATATGGCCTCTTCTTTAGGCTCCACCTTGGCAGATCTTTGAAGAGGCTAGGTCGCCGCGTCTGCAACTCCGAGCATGTTCATGGCTGGGACATAAAACCAGTGAGGTTTGAGCTATCAACTTCTAATGGTCACCGTGCTGTATCCCAATGTTACTTGGACAATCCTGGAAACTGGATCAATTACCATGTGGGAGATTTTGTTGTTGACAATCCTCATGATTTGATCAAGATCAAGTACTCGATGACTCAGATCGATTGCACTCACACTAAAGGCGGTGTCTGCGTGGATTCGGTGTTGATATACCCTAGTAGTGTAGCGAAAGAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

152

Amino Acids

17.56

Weight (kDa)

6.69

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 15 - 144 4.5e-27 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012773)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61060 AT3G61060
fragaria_vesca FvH4_7g13220
malus_domestica MD01G1050900.v1.1
prunus_persica Prupe.2G156400_v2.0.a1
pyrus_communis pycom01g07570
rosa_chinensis RchiOBHm_Chr1g0352361
rosa_laevigata RLG00000028336
rosa_multiflora Rmu_co8012550.1_g000001 Rmu_sc0002244.1_g000039 Rmu_ssc0000172.1_g000009
rosa_roxburghii Rroxscaffold_4G00302960
rosa_rugosa Rorug01G0227900 Rorug01G0228000
rosa_samantha Rh1BG212400 Rh1DG238800
rosa_wichuraiana Rw1G020910 Rw1G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 183
AciI CCGC 2 cut(s) 181, 399
AclWI GGATC 1 cut(s) 308
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 3 cut(s) 86, 110, 166
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
AlwI GGATC 1 cut(s) 308
AoxI GGCC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 291
BciVI GTATCC 1 cut(s) 277
BclI TGATCA 1 cut(s) 348
BfaI CTAG 2 cut(s) 173, 432
BfuI GTATCC 1 cut(s) 277
BglII AGATCT 1 cut(s) 158
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 378
BsaBI GATNNNNATC 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 151
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 309, 339
Bse1I ACTGG 2 cut(s) 224, 302
Bse8I GATNNNNATC 1 cut(s) 353
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 353
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 224, 302
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 132
BshVI ATCGAT 1 cut(s) 378
BslFI GGGAC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 1 cut(s) 132
Bsp143I GATC 5 cut(s) 158, 300, 348, 354, 375
BspACI CCGC 2 cut(s) 181, 399
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 146
BspPI GGATC 1 cut(s) 308
BsrI ACTGG 2 cut(s) 224, 302
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 5 cut(s) 158, 300, 348, 354, 375
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 55, 260
Bst6I CTCTTC 2 cut(s) 140, 161
BstDEI CTNAG 2 cut(s) 372, 453
BstEII GGTNACC 1 cut(s) 254
BstFNI CGCG 1 cut(s) 183
BstKTI GATC 5 cut(s) 161, 303, 351, 357, 378
BstMBI GATC 5 cut(s) 158, 300, 348, 354, 375
BstMWI GCNNNNNNNGC 2 cut(s) 186, 405
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 203
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 289
BstUI CGCG 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 1 cut(s) 132
BsuTUI ATCGAT 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 231
CciI TCATGA 1 cut(s) 340
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 361
CspCI CAANNNNNGTGG 2 cut(s) 59, 94
CviAII CATG 4 cut(s) 200, 206, 311, 341
CviJI RGCY 6 cut(s) 132, 145, 172, 210, 238, 451
CviKI_1 RGCY 6 cut(s) 132, 145, 172, 210, 238, 451
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 2 cut(s) 372, 453
DpnI GATC 5 cut(s) 160, 302, 350, 356, 377
DpnII GATC 5 cut(s) 158, 300, 348, 354, 375
Eam1104I CTCTTC 2 cut(s) 140, 161
EarI CTCTTC 2 cut(s) 140, 161
Eco130I CCWWGG 1 cut(s) 151
Eco91I GGTNACC 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 203, 209, 314, 344
FaiI YATR 8 cut(s) 17, 129, 201, 207, 218, 312, 342, 427
FaqI GGGAC 1 cut(s) 227
FatI CATG 4 cut(s) 199, 205, 310, 340
FbaI TGATCA 1 cut(s) 348
Fnu4HI GCNGC 1 cut(s) 181
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 2 cut(s) 173, 432
GluI GCNGC 1 cut(s) 181
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 1 cut(s) 132
HgaI GACGC 1 cut(s) 172
Hin1II CATG 4 cut(s) 203, 209, 314, 344
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 4 cut(s) 8, 45, 370, 413
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 13, 196, 375
Hpy188III TCNNGA 3 cut(s) 49, 341, 352
Hpy8I GTNNAC 1 cut(s) 331
HpyCH4III ACNGT 2 cut(s) 55, 260
HpyCH4V TGCA 2 cut(s) 189, 384
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 405
HpyF3I CTNAG 2 cut(s) 372, 453
Hsp92II CATG 4 cut(s) 203, 209, 314, 344
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 5 cut(s) 158, 300, 348, 354, 375
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 7 cut(s) 61, 62, 196, 237, 276, 283, 303
MaeI CTAG 2 cut(s) 173, 432
MaeIII GTNAC 2 cut(s) 254, 275
MalI GATC 5 cut(s) 160, 302, 350, 356, 377
MboI GATC 5 cut(s) 158, 300, 348, 354, 375
MboII GAAGA 3 cut(s) 17, 127, 178
MflI RGATCY 1 cut(s) 158
MluCI AATT 2 cut(s) 110, 304
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 4 cut(s) 143, 162, 222, 348
MslI CAYNNNNRTG 1 cut(s) 204
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 186, 405
NdeII GATC 5 cut(s) 158, 300, 348, 354, 375
NlaIII CATG 4 cut(s) 203, 209, 314, 344
NlaIV GGNNCC 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 254
NspI RCATGY 1 cut(s) 203
PagI TCATGA 1 cut(s) 340
PfeI GAWTC 3 cut(s) 8, 45, 413
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 289
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 158
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 204
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 5 cut(s) 158, 300, 348, 354, 375
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 291
SetI ASST 4 cut(s) 153, 178, 233, 240
SmiMI CAYNNNNRTG 1 cut(s) 204
Sse9I AATT 2 cut(s) 110, 304
SsiI CCGC 2 cut(s) 181, 399
SspMI CTAG 2 cut(s) 173, 432
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 55, 260
TaqI TCGA 2 cut(s) 365, 378
TasI AATT 2 cut(s) 110, 304
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 3 cut(s) 8, 45, 413
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 231
XceI RCATGY 1 cut(s) 203
XspI CTAG 2 cut(s) 173, 432
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.