RLG00000028336

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012773
Physical Location & Seq
Forward (+)
21559306 .. 21560520
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028336

Sequence Viewer

Length: 780 bp
ATGGGTGCTAATTTATCGGGGGGTGTTTCGGACACGGACGGTTATGCGCAGCCGAGGCTTGGGGATATACCAGAGAGCTGTGTGGCATTGGTTTTGATGTACCTGGACCCTCCTGAGATTTGCAAACTGGCCCGGTTGAACCGGGCTTTTCGCGGTGCTTCGTGGGCCGATTTCATCTGGGAATCGAAGCTGCCCACAAATTACCGGTTTATTGTGGACAAGGTGTTCGATGAAAAGTCCATGGTGAAGAATTCTGGGATGAGGGATATCTATACCACGCTTTGCAAGTCTATTTCCTTTGATGATGGAACAAAGGAAACCAGCAAGAGCTTCAATTGTGTATCTTTGTCCCAGTTTCAGACAGTGGCTTATCTCCAACAAACCTGGTGGTTTGAAGTTAATGGAGAGTTTGACTTTCAATTTCCGTTAGGGAAATATAGCCTCTTCTTTAGGCTCCACCTTGGCAGATCCTCCAAGAGACTTGGTCGCCGAGTATGCAATTCGGAGCATGTTCATGGCTGGAACATAAAACCAGTGAAGTTTGAGCTTACAACTTCTAATGGTCACCAGGCTGTATCAGAATATTACTTGGACAACCCTGGAAACTGGGTCAATTACCATGTGGGAGACTTTGTTGTTGACAATCCTCATGCTTTGATCAAGATCAAATATTCGATGACCCAGATCGATTGTACTCACACCAAAGGCGGTGTCTGTGTAGACTCTGTGTTGATACGCCCTAGTAGTGTACGTCCTAGTAGTGTAGGAAAAGAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

260

Amino Acids

29.34

Weight (kDa)

8.11

Isoelectric Point (pI)

23.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 108 - 247 1.6e-27 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012773)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61060 AT3G61060
fragaria_vesca FvH4_7g13220
malus_domestica MD01G1050900.v1.1
prunus_persica Prupe.2G156400_v2.0.a1
pyrus_communis pycom01g07570
rosa_chinensis RchiOBHm_Chr1g0352361
rosa_laevigata RLG00000028336
rosa_multiflora Rmu_co8012550.1_g000001 Rmu_sc0002244.1_g000039 Rmu_ssc0000172.1_g000009
rosa_roxburghii Rroxscaffold_4G00302960
rosa_rugosa Rorug01G0227900 Rorug01G0228000
rosa_samantha Rh1BG212400 Rh1DG238800
rosa_wichuraiana Rw1G020910 Rw1G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 48
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 153
AciI CCGC 2 cut(s) 153, 708
AclWI GGATC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 250
AfaI GTAC 3 cut(s) 101, 694, 750
AfiI CCNNNNNNNGG 1 cut(s) 59
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 4 cut(s) 139, 334, 395, 419
AjnI CCWGG 4 cut(s) 102, 383, 567, 598
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 4 cut(s) 78, 190, 330, 547
AluI AGCT 4 cut(s) 78, 190, 330, 547
Alw26I GTCTC 2 cut(s) 472, 621
AlwI GGATC 1 cut(s) 462
AoxI GGCC 2 cut(s) 129, 165
ApeKI GCWGC 2 cut(s) 49, 190
ApoI RAATTY 1 cut(s) 250
ArsI GACNNNNNNTTYG 4 cut(s) 209, 241, 696, 728
AsiGI ACCGGT 1 cut(s) 204
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 106, 130, 165
AsuC2I CCSGG 2 cut(s) 133, 143
AsuHPI GGTGA 2 cut(s) 256, 557
AvaII GGWCC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 61, 177
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 4 cut(s) 104, 385, 569, 600
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 2 cut(s) 133, 143
BcoDI GTCTC 2 cut(s) 472, 621
BfaI CTAG 2 cut(s) 741, 756
BisI GCNGC 2 cut(s) 50, 191
BlsI GCNGC 2 cut(s) 51, 192
Bme1390I CCNGG 6 cut(s) 104, 133, 143, 385, 569, 600
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 106, 130, 165
BmiI GGNNCC 2 cut(s) 108, 455
BmrFI CCNGG 6 cut(s) 104, 133, 143, 385, 569, 600
BmrI ACTGGG 2 cut(s) 346, 616
BmuI ACTGGG 2 cut(s) 346, 616
BpuMI CCSGG 2 cut(s) 133, 143
Bsa29I ATCGAT 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 53, 240, 460, 598
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 4 cut(s) 132, 352, 533, 611
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 4 cut(s) 104, 385, 569, 600
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 4 cut(s) 53, 240, 460, 598
BseGI GGATG 1 cut(s) 264
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 4 cut(s) 132, 352, 533, 611
BseXI GCAGC 2 cut(s) 61, 177
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 131, 167
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 687
BsiSI CCGG 3 cut(s) 133, 142, 205
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 59
BsmAI GTCTC 2 cut(s) 472, 621
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 2 cut(s) 131, 167
Bsp143I GATC 4 cut(s) 467, 657, 663, 684
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 2 cut(s) 153, 708
BspANI GGCC 2 cut(s) 131, 167
BspCNI CTCAG 1 cut(s) 106
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 108, 455
BspPI GGATC 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 4 cut(s) 132, 352, 533, 611
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 4 cut(s) 53, 240, 460, 598
BssMI GATC 4 cut(s) 467, 657, 663, 684
BssT1I CCWWGG 2 cut(s) 240, 460
Bst2UI CCWGG 4 cut(s) 104, 385, 569, 600
Bst4CI ACNGT 2 cut(s) 41, 364
Bst6I CTCTTC 1 cut(s) 449
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 240
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 1 cut(s) 264
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 4 cut(s) 470, 660, 666, 687
BstMAI GTCTC 2 cut(s) 472, 621
BstMBI GATC 4 cut(s) 467, 657, 663, 684
BstMWI GCNNNNNNNGC 3 cut(s) 55, 164, 495
BstNI CCWGG 4 cut(s) 104, 385, 569, 600
BstNSI RCATGY 1 cut(s) 512
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 6 cut(s) 102, 131, 141, 383, 567, 598
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 61, 177
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 2 cut(s) 131, 167
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 264
BtsIMutI CAGTG 2 cut(s) 369, 540
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 106, 130, 165
ClaI ATCGAT 1 cut(s) 687
CsiI ACCWGGT 1 cut(s) 383
Csp6I GTAC 3 cut(s) 100, 693, 749
CspAI ACCGGT 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 368, 403
CviAII CATG 5 cut(s) 241, 509, 515, 620, 650
CviQI GTAC 3 cut(s) 100, 693, 749
DdeI CTNAG 1 cut(s) 114
DpnI GATC 4 cut(s) 469, 659, 665, 686
DpnII GATC 4 cut(s) 467, 657, 663, 684
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 240, 460
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 4 cut(s) 102, 383, 567, 598
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 240, 460
ErhI CCWWGG 2 cut(s) 240, 460
FaeI CATG 5 cut(s) 244, 512, 518, 623, 653
FaqI GGGAC 1 cut(s) 334
FatI CATG 5 cut(s) 240, 508, 514, 619, 649
FbaI TGATCA 1 cut(s) 657
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 2 cut(s) 50, 191
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 50, 191
FspBI CTAG 2 cut(s) 741, 756
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 50, 191
HaeIII GGCC 2 cut(s) 131, 167
HapII CCGG 3 cut(s) 133, 142, 205
HhaI GCGC 1 cut(s) 49
Hin1II CATG 5 cut(s) 244, 512, 518, 623, 653
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 2 cut(s) 182, 722
HpaII CCGG 3 cut(s) 133, 142, 205
HphI GGTGA 2 cut(s) 256, 557
Hpy166II GTNNAC 4 cut(s) 217, 640, 721, 749
Hpy188I TCNGA 4 cut(s) 31, 360, 505, 580
Hpy188III TCNNGA 2 cut(s) 113, 661
Hpy8I GTNNAC 4 cut(s) 217, 640, 721, 749
HpyCH4III ACNGT 2 cut(s) 41, 364
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 3 cut(s) 123, 285, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 164, 495
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 5 cut(s) 244, 512, 518, 623, 653
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 4 cut(s) 467, 657, 663, 684
LmnI GCTCC 2 cut(s) 459, 505
Lsp1109I GCAGC 2 cut(s) 61, 177
MabI ACCWGGT 1 cut(s) 383
MaeI CTAG 2 cut(s) 741, 756
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 1 cut(s) 563
MalI GATC 4 cut(s) 469, 659, 665, 686
MboI GATC 4 cut(s) 467, 657, 663, 684
MboII GAAGA 2 cut(s) 259, 436
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 1 cut(s) 467
MluCI AATT 7 cut(s) 10, 199, 250, 334, 419, 499, 613
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 6 cut(s) 48, 120, 255, 452, 481, 657
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 3 cut(s) 133, 142, 205
MspR9I CCNGG 6 cut(s) 104, 133, 143, 385, 569, 600
MunI CAATTG 1 cut(s) 334
MvaI CCWGG 4 cut(s) 104, 385, 569, 600
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 55, 164, 495
NciI CCSGG 2 cut(s) 133, 143
NcoI CCATGG 1 cut(s) 240
NdeII GATC 4 cut(s) 467, 657, 663, 684
NlaIII CATG 5 cut(s) 244, 512, 518, 623, 653
NlaIV GGNNCC 2 cut(s) 108, 455
NmeAIII GCCGAG 2 cut(s) 78, 515
NmuCI GTSAC 1 cut(s) 563
NsbI TGCGCA 1 cut(s) 48
NspI RCATGY 1 cut(s) 512
PfeI GAWTC 1 cut(s) 182
PflFI GACNNNGTC 1 cut(s) 483
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 2 cut(s) 51, 192
PleI GAGTC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 716
Psp6I CCWGG 4 cut(s) 102, 383, 567, 598
PspEI GGTNACC 1 cut(s) 563
PspGI CCWGG 4 cut(s) 102, 383, 567, 598
PspN4I GGNNCC 2 cut(s) 108, 455
PspPI GGNCC 3 cut(s) 106, 130, 165
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 483
RsaI GTAC 3 cut(s) 101, 694, 750
RsaNI GTAC 3 cut(s) 100, 693, 749
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 2 cut(s) 50, 191
Sau3AI GATC 4 cut(s) 467, 657, 663, 684
Sau96I GGNCC 3 cut(s) 106, 130, 165
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 6 cut(s) 104, 133, 143, 385, 569, 600
SetI ASST 9 cut(s) 80, 105, 192, 225, 332, 386, 462, 549, 754
SexAI ACCWGGT 1 cut(s) 383
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 513
Sse9I AATT 7 cut(s) 10, 199, 250, 334, 419, 499, 613
SsiI CCGC 2 cut(s) 153, 708
SspI AATATT 2 cut(s) 584, 671
SspMI CTAG 2 cut(s) 741, 756
StyD4I CCNGG 6 cut(s) 102, 131, 141, 383, 567, 598
StyI CCWWGG 2 cut(s) 240, 460
TaaI ACNGT 2 cut(s) 41, 364
TaiI ACGT 1 cut(s) 754
TaqI TCGA 4 cut(s) 185, 228, 674, 687
TasI AATT 7 cut(s) 10, 199, 250, 334, 419, 499, 613
TatI WGTACW 1 cut(s) 692
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 2 cut(s) 369, 540
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 2 cut(s) 49, 190
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 3 cut(s) 163, 246, 503
TspGWI ACGGA 2 cut(s) 50, 414
TspRI CASTG 2 cut(s) 369, 540
Tth111I GACNNNGTC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 250
XceI RCATGY 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 2 cut(s) 741, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.