Rroxscaffold_4G00302960

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012773
Physical Location & Seq
Forward (+)
23302323 .. 23304198
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302960.1

Sequence Viewer

Length: 912 bp
ATGGGTGCTAATTTATCGGGGGGTGTTTCGGACACGGAGGGTTATGCGCAGCCGAGGCTTGGGGATATACCAGAGAGCTGTGTGGCGTTGGTTTTGATGTACATGGACCCTCCTGAGATTTGCAAATTGGCCCGGTTGAACCGGGCTTTTCGCGGTGCTTCGTGGGCCAATTTCATCTGGGAATCGAAGTTGCCCACAAATTACCGGTATATTGTAGACAAGGTGTTCGATGAAAAGTCTATGGTGAAAAATTCAGGGATGAGGGATATCTATACCACGCTTTGCAAGTCTATTTCCTTTGATGATGGAACAAAGCAAATTTGGCTAGATAAGACCAAGGGTGGTATTCATCTGTCGATTTCTTCAAAGGCGTTATCGATTACAGGGATAGATGATCGAAGATATTGGAATTTCATAGCGATTGATGAATCAAGGAAACCAGCAAGGGCTTCAATTGAGTATCTTTGTCCCAGTTTGCGAGCCTTGCCCCTTTTTGATATGTTTCAGACAGTGGCTTATCTCCAACAAACCTGGTGGTTTGAAGTTAATGGAGAGTTTGACTTTCAATTTCCATTAGGGAAATATAGCCTCTTCTTTAGGCTCCACCTTGGCAGATCCTCCAAGAGACTTGGTCGCCGAGTATGCAATTCGGAGCATGTTCATGGCTGGAACATAAAACCAGTGAAGTTTGAGCTTACAACTTCTAATGGTCACCAGGCTGTATCAGAATATTACTTGGACAACCCTGGAAACTGGGTCAATTACCATGTGGGAGACTTTGTTGTTGACAATCCTCATGCTTTGATCAAGATCAAATATTCGATGACCCAGATCGATTGTACTCACACCAAAGGCGGTGTCTGTGTAGACTCTGTGTTGATACGCCCTAGTAGTGTAGGAAAAGAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

303

Amino Acids

34.51

Weight (kDa)

8.57

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 167 - 296 4.5e-26 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012773)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61060 AT3G61060
fragaria_vesca FvH4_7g13220
malus_domestica MD01G1050900.v1.1
prunus_persica Prupe.2G156400_v2.0.a1
pyrus_communis pycom01g07570
rosa_chinensis RchiOBHm_Chr1g0352361
rosa_laevigata RLG00000028336
rosa_multiflora Rmu_co8012550.1_g000001 Rmu_sc0002244.1_g000039 Rmu_ssc0000172.1_g000009
rosa_roxburghii Rroxscaffold_4G00302960
rosa_rugosa Rorug01G0227900 Rorug01G0228000
rosa_samantha Rh1BG212400 Rh1DG238800
rosa_wichuraiana Rw1G020910 Rw1G021070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 48
AccI GTMKAC 2 cut(s) 216, 867
AccII CGCG 1 cut(s) 153
AciI CCGC 2 cut(s) 153, 855
AclWI GGATC 1 cut(s) 609
AcsI RAATTY 3 cut(s) 250, 318, 409
AfaI GTAC 2 cut(s) 101, 841
AfiI CCNNNNNNNGG 1 cut(s) 59
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 5 cut(s) 139, 366, 453, 542, 566
AjnI CCWGG 3 cut(s) 530, 714, 745
AluBI AGCT 2 cut(s) 78, 694
AluI AGCT 2 cut(s) 78, 694
Alw26I GTCTC 2 cut(s) 619, 768
AlwI GGATC 1 cut(s) 609
AoxI GGCC 2 cut(s) 129, 165
ApeKI GCWGC 1 cut(s) 49
ApoI RAATTY 3 cut(s) 250, 318, 409
ArsI GACNNNNNNTTYG 4 cut(s) 209, 241, 843, 875
AsiGI ACCGGT 1 cut(s) 204
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 106, 130, 165
AsuC2I CCSGG 2 cut(s) 133, 143
AsuHPI GGTGA 2 cut(s) 256, 704
AvaII GGWCC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 61
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 3 cut(s) 532, 716, 747
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 2 cut(s) 133, 143
BcoDI GTCTC 2 cut(s) 619, 768
BfaI CTAG 2 cut(s) 326, 888
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 5 cut(s) 133, 143, 532, 716, 747
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 3 cut(s) 106, 130, 165
BmiI GGNNCC 2 cut(s) 108, 602
BmrFI CCNGG 5 cut(s) 133, 143, 532, 716, 747
BmrI ACTGGG 2 cut(s) 465, 763
BmuI ACTGGG 2 cut(s) 465, 763
BpuMI CCSGG 2 cut(s) 133, 143
Bsa29I ATCGAT 2 cut(s) 377, 834
BsaBI GATNNNNATC 1 cut(s) 809
BsaJI CCNNGG 4 cut(s) 53, 336, 607, 745
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 3 cut(s) 471, 680, 758
Bse8I GATNNNNATC 1 cut(s) 809
BseBI CCWGG 3 cut(s) 532, 716, 747
BseCI ATCGAT 2 cut(s) 377, 834
BseDI CCNNGG 4 cut(s) 53, 336, 607, 745
BseGI GGATG 1 cut(s) 264
BseJI GATNNNNATC 1 cut(s) 809
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 3 cut(s) 471, 680, 758
BseXI GCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 131, 167
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 2 cut(s) 377, 834
BsiSI CCGG 3 cut(s) 133, 142, 205
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 59
BsmAI GTCTC 2 cut(s) 619, 768
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 2 cut(s) 131, 167
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 5 cut(s) 394, 614, 804, 810, 831
BspACI CCGC 2 cut(s) 153, 855
BspANI GGCC 2 cut(s) 131, 167
BspCNI CTCAG 1 cut(s) 106
BspDI ATCGAT 2 cut(s) 377, 834
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 108, 602
BspPI GGATC 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 471, 680, 758
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 4 cut(s) 53, 336, 607, 745
BssMI GATC 5 cut(s) 394, 614, 804, 810, 831
BssT1I CCWWGG 2 cut(s) 336, 607
Bst2UI CCWGG 3 cut(s) 532, 716, 747
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 596
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 1 cut(s) 114
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 1 cut(s) 264
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 5 cut(s) 397, 617, 807, 813, 834
BstMAI GTCTC 2 cut(s) 619, 768
BstMBI GATC 5 cut(s) 394, 614, 804, 810, 831
BstMWI GCNNNNNNNGC 5 cut(s) 55, 164, 322, 484, 642
BstNI CCWGG 3 cut(s) 532, 716, 747
BstNSI RCATGY 1 cut(s) 659
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 131, 141, 530, 714, 745
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
Bsu15I ATCGAT 2 cut(s) 377, 834
BsuRI GGCC 2 cut(s) 131, 167
BsuTUI ATCGAT 2 cut(s) 377, 834
BtsCI GGATG 1 cut(s) 264
BtsIMutI CAGTG 2 cut(s) 516, 687
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 106, 130, 165
ClaI ATCGAT 2 cut(s) 377, 834
CsiI ACCWGGT 1 cut(s) 530
Csp6I GTAC 2 cut(s) 100, 840
CspAI ACCGGT 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 515, 550
CviAII CATG 5 cut(s) 103, 656, 662, 767, 797
CviQI GTAC 2 cut(s) 100, 840
DdeI CTNAG 1 cut(s) 114
DpnI GATC 5 cut(s) 396, 616, 806, 812, 833
DpnII GATC 5 cut(s) 394, 614, 804, 810, 831
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco130I CCWWGG 2 cut(s) 336, 607
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 3 cut(s) 530, 714, 745
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 336, 607
ErhI CCWWGG 2 cut(s) 336, 607
FaeI CATG 5 cut(s) 106, 659, 665, 770, 800
FaqI GGGAC 1 cut(s) 453
FatI CATG 5 cut(s) 102, 655, 661, 766, 796
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 2 cut(s) 216, 867
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 50
FspBI CTAG 2 cut(s) 326, 888
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 50
HaeIII GGCC 2 cut(s) 131, 167
HapII CCGG 3 cut(s) 133, 142, 205
HhaI GCGC 1 cut(s) 49
Hin1II CATG 5 cut(s) 106, 659, 665, 770, 800
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 3 cut(s) 182, 428, 869
HpaII CCGG 3 cut(s) 133, 142, 205
HphI GGTGA 2 cut(s) 256, 704
Hpy166II GTNNAC 3 cut(s) 217, 787, 868
Hpy188I TCNGA 4 cut(s) 31, 507, 652, 727
Hpy188III TCNNGA 2 cut(s) 113, 808
Hpy8I GTNNAC 3 cut(s) 217, 787, 868
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4V TGCA 3 cut(s) 123, 285, 645
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 164, 322, 484, 642
HpyF3I CTNAG 1 cut(s) 114
Hsp92II CATG 5 cut(s) 106, 659, 665, 770, 800
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 5 cut(s) 394, 614, 804, 810, 831
LmnI GCTCC 2 cut(s) 606, 652
Lsp1109I GCAGC 1 cut(s) 61
MabI ACCWGGT 1 cut(s) 530
MaeI CTAG 2 cut(s) 326, 888
MaeIII GTNAC 1 cut(s) 710
MalI GATC 5 cut(s) 396, 616, 806, 812, 833
MboI GATC 5 cut(s) 394, 614, 804, 810, 831
MboII GAAGA 3 cut(s) 354, 411, 583
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 614
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 7 cut(s) 31, 48, 120, 255, 599, 628, 804
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 660
MspI CCGG 3 cut(s) 133, 142, 205
MspR9I CCNGG 5 cut(s) 133, 143, 532, 716, 747
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 3 cut(s) 532, 716, 747
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 5 cut(s) 55, 164, 322, 484, 642
NciI CCSGG 2 cut(s) 133, 143
NdeII GATC 5 cut(s) 394, 614, 804, 810, 831
NlaIII CATG 5 cut(s) 106, 659, 665, 770, 800
NlaIV GGNNCC 2 cut(s) 108, 602
NmeAIII GCCGAG 2 cut(s) 78, 662
NmuCI GTSAC 1 cut(s) 710
NsbI TGCGCA 1 cut(s) 48
NspI RCATGY 1 cut(s) 659
PfeI GAWTC 2 cut(s) 182, 428
PflFI GACNNNGTC 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 1 cut(s) 51
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
Psp6I CCWGG 3 cut(s) 530, 714, 745
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 3 cut(s) 530, 714, 745
PspN4I GGNNCC 2 cut(s) 108, 602
PspPI GGNCC 3 cut(s) 106, 130, 165
PsuI RGATCY 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 630
RsaI GTAC 2 cut(s) 101, 841
RsaNI GTAC 2 cut(s) 100, 840
RseI CAYNNNNRTG 1 cut(s) 660
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 5 cut(s) 394, 614, 804, 810, 831
Sau96I GGNCC 3 cut(s) 106, 130, 165
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 5 cut(s) 133, 143, 532, 716, 747
SetI ASST 5 cut(s) 80, 225, 533, 609, 696
SexAI ACCWGGT 1 cut(s) 530
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 660
SsiI CCGC 2 cut(s) 153, 855
SspI AATATT 2 cut(s) 731, 818
SspMI CTAG 2 cut(s) 326, 888
StyD4I CCNGG 5 cut(s) 131, 141, 530, 714, 745
StyI CCWWGG 2 cut(s) 336, 607
TaaI ACNGT 1 cut(s) 511
TaqI TCGA 7 cut(s) 185, 228, 356, 377, 397, 821, 834
TatI WGTACW 2 cut(s) 99, 839
TfiI GAWTC 2 cut(s) 182, 428
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 2 cut(s) 516, 687
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 6 cut(s) 163, 246, 338, 403, 441, 650
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 2 cut(s) 516, 687
Tth111I GACNNNGTC 1 cut(s) 630
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 3 cut(s) 250, 318, 409
XceI RCATGY 1 cut(s) 659
XmiI GTMKAC 2 cut(s) 216, 867
XspI CTAG 2 cut(s) 326, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.