MD01G1066300.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011114
Physical Location & Seq
Reverse (-)
17014839 .. 17018076
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1066300.v1.1.491

Sequence Viewer

Length: 2739 bp
ATGAATGAAATTTCACCACGGCCGCCGTCGCCGCCACCCGCCATGGCTTCTTCCATGCTGCCCATTTCTCTTCTCCTTCTTCTTCTCCAAGCCCTTCTTCTAAAACCCATAAACGCCATCCCCGTTGACGAATTGTCACCCCGAATCGAATCCCAGTTCTACACCAGAGCCAAAGTCCCCGACTTTCCTGACTCCCCCACGCGCAGCACAAACGGGTCTCCCGTACCCGCGACGGCGACGGCGACGCTCCCGAATTTACAGTACCCAATGGCGAGGAACAGCGAAGTTGTAATCTTTGGGAACGCGACGCTCTCGAGCCCCAACCGGACCTTTGAGCTCGGCTTCTTCTCCACCAACAACGAAAGTCCCAAATGGTACTTGGGCATTTGGTATGCTCTGATTCCGGTCCCGACTTACGTCTGGGTCGCGAACCGGGGCCGAGCCGTCAATAATCTCACCTCCTCGAGCTTGGAAATCACCGGGTCGGGTCGGTTGGTGGTCAAAGAATCCGGTAACTCAATCGTGTGGTCGAGCACGAACGCGGGGATCGGCGTTAGTGTCGAGCTTTTGGAGAATGGCAATCTGGTTTTGCTTACCCAGGGAGGCACCGTCGCGTGGCAGAGCTTCGATTACCCGACTGACACGTGGCTTCCGGGGATGAACCTGACGAGCGAGCGAGGGCTTACGTGTTGGCGGAGCTACATAGACCCTTCGCCGGGTTTGTATTCTTTGCGGCTGAGACCGCCGGAGTACGGTGAGTTTGAGCTCGTTTTTAATGGTACTGCATCTTATTGGACAACTGGGAATTGGACCGGTAATGCCTTCGCTAATGTGCCTCAAATGACGGTGCCTTACATATATAAGTTCCATTTTATCGACCCATTCACGCCAACGGCGTCGTTCGGGTTCACTGAGTCGCCGCTGGATGGCCGGTTGGACCCGCCGCTGACCCGGTTCCAGATGGACCCTTCGGGTCAGCTCAGGCAGTTCACTTGGGCAACGCAGACCTGGAACACGTTTTGGTTTCAGCCTGAGAAAAAGTGCAGAGTGTTTGGTATGTGTGGGCCTTTTGGGGTTTGCAATGGTGAAACACCTAGGCCATGTGAGTGTGACTCCGGTTTTCAGCCGGTGGATGAGTCTAGTTGGAACTTAGGGGACTACTCGGGGGGTTGCCGCCGGGTGTATAATGAGGGAGTGGCTTGTGGTCAGAGGGATGATAGGTTTGACAAGGTTGGTGTTGTGAGATATGTTGGAAGTTATAGTAAGCCTTATAGAACTGACTTTGACGATTGTCAGGACATATGCTTGCTTTTATGTACTTGTATAGGTGTGGACTACGATAACATTACTGGCATCTGTAGAGTGTATAACGGGTCTCTCTCGAATTTGCAAAATTTGACCTCCGAAAGTGGATTTGTTAATGATTTCTATTTAAGGGTGCAAAAAAGTGCAACGAGATTGGAGAAGAAGGTGCCAATTCGTGTTGTTTTGAGTGCTAGCATTGTTGGGTCAATTGTAATTTTGGGCTTTGTAGCGGCGGTGGTGGTGATTTTTATTAAGGTAAGAGAGAAGAAGAAGAGAGAAAAAGAAAAGGTTCAGCTTATGTCAACCTTTAATTTGAAGGTATTTTCTTATAAAGAACTTGACACTGCAACACGGGGTTTTTCAGACAAACTTGGGCATGGTGGATTTGGAGCTGTTTTCCACGGGGAGTTATTAGATTCTACTATTGTTGCAGTGAAGCGCTTAGATAGGCCAGGTGGTGGACAGAAAGAATTCCAAGCGGAAGTTTGCACGATTGGGAACATTCAACATGTCAATCTTGTCAGACTTAGAGGCTTCTGCTCAGAAAATTCCCATAGACTTTTGGTTTATGATTACATGCCAAATGGTGCTCTTAGTGTGTACCTACGTCAAGATGGTCCAAATTTGAGTTGGGATGTTAGATTTCGGATAGCAGTTGGTATGGCAAGAGGCATTGCATATTTACATGAGGAGTGTAGAGAGTGCATAATCCATTGTGATATCAAACCAGAAAACATTTTGCTTGATAGTTCTTGCACTGCAAAGGTGTCAGATTTTGGGTTGGCAAAGCTAGTTGTACGGGACTTTAGTCGGGTATTAGCCACAATGAGGGGCACCAGGGGTTATATGGCACCAGAGTGGATATCTGGTGTGGCAATCACCATGAAAGCCGATGTATACAGCTATGGAATGACACTAATAGAATTACTTGCAGGCCGCAGAAATGTGGAGGCGCGCCAATCTGTAGGTGGAAGAGAGGGTGCAAGTGAAACAACTGACAGTTTGTTCTTTCCTCCACGGGCGGCACAGAAAATAATTGAGGGCAATGTAAGGGAAGTTATTGACAATAGGCTCGGATGCACATATAATATTGAGGAGGCAGAGCGTGCTGCAATGGTGGCAGTGTGGTGCATACAGGATGCCGAGGCAATGCGGCCTACCATGGGGATGGTAGTGAAAATGTTGGAAGGGCTGGTGGAAGTGACTGTTCCTCCAGCACCCAAATTGTTGCAAGCACTGGTTTCAGGGGAGTCTTTTTGCGGGGTAAAGGCTGATTCGGGAATTGGGTTATCTAATGGCAGCGACTTCTCCGGTTACAATACAAGATTATCGAGTGGTTGTTCAGAATCGTCTATTGGTAATGGCTCTTCCCTAGTGACTGAGATTGTGGATGATAACCAAAGAAGTTCAGTGGGCTTTACCGACCAAGCCTAA

Protein Analysis

913

Amino Acids

100.49

Weight (kDa)

5.9

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 138 - 225 3.6e-24 D-mannose binding lectin
S_locus_glycop PF00954 265 - 376 1.4e-21 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 556 - 750 5.3e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 557 - 826 5.4e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1635
AccB1I GGYRCC 5 cut(s) 605, 847, 1471, 2138, 2155
AccB7I CCANNNNNTGG 1 cut(s) 1763
AccI GTMKAC 1 cut(s) 2202
AccII CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 2 cut(s) 20, 928
AcsI RAATTY 7 cut(s) 9, 253, 1384, 1393, 1775, 1852, 1927
AcvI CACGTG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 896
AfaI GTAC 8 cut(s) 225, 263, 377, 752, 781, 1318, 1907, 2103
AfeI AGCGCT 1 cut(s) 1745
AfiI CCNNNNNNNGG 8 cut(s) 615, 715, 716, 752, 926, 1763, 2133, 2468
AflIII ACRYGT 4 cut(s) 642, 686, 1014, 1813
AgeI ACCGGT 1 cut(s) 812
AgsI TTSAA 2 cut(s) 1621, 1811
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 4 cut(s) 597, 1007, 1756, 2141
AjuI GAANNNNNNNTTGG 4 cut(s) 81, 113, 2643, 2675
AleI CACNNNNGTG 1 cut(s) 2161
Alw21I GWGCWC 4 cut(s) 339, 536, 768, 1897
Alw26I GTCTC 3 cut(s) 222, 733, 1380
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 3 cut(s) 313, 463, 1162
Aor51HI AGCGCT 1 cut(s) 1745
AoxI GGCC 8 cut(s) 20, 436, 928, 1064, 1097, 1754, 2239, 2459
ApeKI GCWGC 4 cut(s) 58, 204, 2414, 2604
ApoI RAATTY 7 cut(s) 9, 253, 1384, 1393, 1775, 1852, 1927
AscI GGCGCGCC 1 cut(s) 2258
AsiGI ACCGGT 1 cut(s) 812
Asp700I GAANNNNTTC 2 cut(s) 1593, 1775
AspA2I CCTAGG 1 cut(s) 1094
AspLEI GCGC 4 cut(s) 204, 1746, 2260, 2262
AspS9I GGNCC 8 cut(s) 327, 406, 436, 810, 937, 964, 1064, 1922
AsuC2I CCSGG 6 cut(s) 434, 481, 654, 717, 952, 1178
AsuHPI GGTGA 8 cut(s) 6, 129, 448, 469, 767, 1097, 1558, 2176
AsuNHI GCTAGC 1 cut(s) 1496
AvaI CYCGRG 3 cut(s) 313, 463, 1162
AvaII GGWCC 6 cut(s) 327, 406, 810, 937, 964, 1922
AvrII CCTAGG 1 cut(s) 1094
BaeGI GKGCMC 1 cut(s) 2141
BanI GGYRCC 5 cut(s) 605, 847, 1471, 2138, 2155
BanII GRGCYC 3 cut(s) 320, 339, 768
BarI GAAGNNNNNNTAC 2 cut(s) 2656, 2688
BbrPI CACGTG 1 cut(s) 645
Bbv12I GWGCWC 4 cut(s) 339, 536, 768, 1897
BbvI GCAGC 4 cut(s) 45, 216, 2401, 2616
BccI CCATC 5 cut(s) 125, 920, 955, 1913, 2467
BceAI ACGGC 6 cut(s) 10, 35, 249, 255, 428, 909
BciT130I CCWGG 4 cut(s) 599, 1009, 1758, 2143
BcnI CCSGG 6 cut(s) 434, 481, 654, 717, 952, 1178
BcoDI GTCTC 3 cut(s) 222, 733, 1380
BfaI CTAG 5 cut(s) 1095, 1140, 1497, 2096, 2678
BfmI CTRYAG 2 cut(s) 1357, 2268
BfoI RGCGCY 1 cut(s) 1747
BlnI CCTAGG 1 cut(s) 1094
Bme18I GGWCC 6 cut(s) 327, 406, 810, 937, 964, 1922
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 3 cut(s) 313, 463, 1162
BmgT120I GGNCC 8 cut(s) 327, 406, 436, 810, 937, 964, 1064, 1922
BmrI ACTGGG 2 cut(s) 148, 810
BmsI GCATC 4 cut(s) 794, 1362, 2372, 2434
BmtI GCTAGC 1 cut(s) 1500
BmuI ACTGGG 2 cut(s) 148, 810
BoxI GACNNNNGTC 3 cut(s) 416, 1290, 2112
BplI GAGNNNNNCTC 2 cut(s) 1097, 1129
BpmI CTGGAG 1 cut(s) 2502
Bpu10I CCTNAGC 1 cut(s) 980
BpuMI CCSGG 6 cut(s) 434, 481, 654, 717, 952, 1178
BsaAI YACGTR 2 cut(s) 645, 687
BsaHI GRCGYC 1 cut(s) 896
BsaI GGTCTC 3 cut(s) 222, 733, 1380
BsaWI WCCGGW 6 cut(s) 324, 403, 509, 812, 1115, 2615
BsaXI ACNNNNNCTCC 4 cut(s) 594, 624, 2500, 2530
Bsc4I CCNNNNNNNGG 8 cut(s) 615, 715, 716, 752, 926, 1763, 2133, 2468
Bse118I RCCGGY 3 cut(s) 812, 930, 1126
Bse1I ACTGG 4 cut(s) 154, 805, 1354, 2547
Bse3DI GCAATG 5 cut(s) 1087, 1977, 2356, 2424, 2460
BseBI CCWGG 4 cut(s) 599, 1009, 1758, 2143
BseLI CCNNNNNNNGG 8 cut(s) 615, 715, 716, 752, 926, 1763, 2133, 2468
BseMI GCAATG 5 cut(s) 1087, 1977, 2356, 2424, 2460
BseMII CTCAG 6 cut(s) 728, 903, 994, 1023, 1860, 2676
BseNI ACTGG 4 cut(s) 154, 805, 1354, 2547
BsePI GCGCGC 1 cut(s) 2258
BseRI GAGGAG 3 cut(s) 451, 2009, 2414
BseSI GKGCMC 1 cut(s) 2141
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 45, 216, 2401, 2616
BsgI GTGCAG 1 cut(s) 1063
Bsh1236I CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 8 cut(s) 22, 438, 930, 1066, 1099, 1756, 2241, 2461
BshNI GGYRCC 5 cut(s) 605, 847, 1471, 2138, 2155
BshTI ACCGGT 1 cut(s) 812
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 4 cut(s) 339, 536, 768, 1897
BsiHKCI CYCGRG 3 cut(s) 313, 463, 1162
BslFI GGGAC 5 cut(s) 161, 351, 392, 1169, 2120
BslI CCNNNNNNNGG 8 cut(s) 615, 715, 716, 752, 926, 1763, 2133, 2468
BsmAI GTCTC 3 cut(s) 222, 733, 1380
BsmFI GGGAC 5 cut(s) 161, 351, 392, 1169, 2120
BsnI GGCC 8 cut(s) 22, 438, 930, 1066, 1099, 1756, 2241, 2461
Bso31I GGTCTC 3 cut(s) 222, 733, 1380
BsoBI CYCGRG 3 cut(s) 313, 463, 1162
Bsp1286I GDGCHC 6 cut(s) 320, 339, 536, 768, 1897, 2141
Bsp143I GATC 1 cut(s) 546
Bsp19I CCATGG 2 cut(s) 42, 2466
Bsp68I TCGCGA 1 cut(s) 428
BspANI GGCC 8 cut(s) 22, 438, 930, 1066, 1099, 1756, 2241, 2461
BspCNI CTCAG 6 cut(s) 729, 904, 993, 1024, 1859, 2677
BspFNI CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
BspOI GCTAGC 1 cut(s) 1500
BspPI GGATC 1 cut(s) 554
BspQI GCTCTTC 1 cut(s) 2677
BspT107I GGYRCC 5 cut(s) 605, 847, 1471, 2138, 2155
BspTNI GGTCTC 3 cut(s) 222, 733, 1380
BsrDI GCAATG 5 cut(s) 1087, 1977, 2356, 2424, 2460
BsrFI RCCGGY 3 cut(s) 812, 930, 1126
BsrI ACTGG 4 cut(s) 154, 805, 1354, 2547
BssAI RCCGGY 3 cut(s) 812, 930, 1126
BssHII GCGCGC 1 cut(s) 2258
BssMI GATC 1 cut(s) 546
BssNAI GTATAC 1 cut(s) 2203
BssNI GRCGYC 1 cut(s) 896
BssT1I CCWWGG 3 cut(s) 42, 1094, 2466
Bst1107I GTATAC 1 cut(s) 2203
Bst2UI CCWGG 4 cut(s) 599, 1009, 1758, 2143
Bst4CI ACNGT 6 cut(s) 261, 610, 755, 847, 2306, 2512
Bst6I CTCTTC 4 cut(s) 75, 1571, 2272, 2677
BstACI GRCGYC 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 2411
BstBAI YACGTR 2 cut(s) 645, 687
BstC8I GCNNGC 7 cut(s) 674, 1307, 1498, 2239, 2260, 2412, 2538
BstDSI CCRYGG 5 cut(s) 17, 42, 1705, 2321, 2466
BstFNI CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
BstH2I RGCGCY 1 cut(s) 1747
BstHHI GCGC 4 cut(s) 204, 1746, 2260, 2262
BstKTI GATC 1 cut(s) 549
BstMAI GTCTC 3 cut(s) 222, 733, 1380
BstMBI GATC 1 cut(s) 546
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 5 cut(s) 28, 31, 742, 2411, 2423
BstNI CCWGG 4 cut(s) 599, 1009, 1758, 2143
BstNSI RCATGY 2 cut(s) 1817, 1885
BstPAI GACNNNNGTC 3 cut(s) 416, 1290, 2112
BstSFI CTRYAG 2 cut(s) 1357, 2268
BstSLI GKGCMC 1 cut(s) 2141
BstUI CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
BstV1I GCAGC 4 cut(s) 45, 216, 2401, 2616
BstXI CCANNNNNNTGG 1 cut(s) 2473
BstZ17I GTATAC 1 cut(s) 2203
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 8 cut(s) 22, 438, 930, 1066, 1099, 1756, 2241, 2461
BtgI CCRYGG 5 cut(s) 17, 42, 1705, 2321, 2466
BtsI GCAGTG 4 cut(s) 1647, 1743, 2061, 2433
BtsIMutI CAGTG 7 cut(s) 909, 1647, 1743, 2061, 2433, 2540, 2721
BtuMI TCGCGA 1 cut(s) 428
Cac8I GCNNGC 7 cut(s) 674, 1307, 1498, 2239, 2260, 2412, 2538
CfoI GCGC 4 cut(s) 204, 1746, 2260, 2262
Cfr10I RCCGGY 3 cut(s) 812, 930, 1126
Cfr13I GGNCC 8 cut(s) 327, 406, 436, 810, 937, 964, 1064, 1922
CseI GACGC 3 cut(s) 253, 316, 885
Csp6I GTAC 8 cut(s) 224, 262, 376, 751, 780, 1317, 1906, 2102
CspAI ACCGGT 1 cut(s) 812
CviAII CATG 9 cut(s) 43, 55, 1101, 1682, 1814, 1882, 1991, 2188, 2467
CviQI GTAC 8 cut(s) 224, 262, 376, 751, 780, 1317, 1906, 2102
DpnI GATC 1 cut(s) 548
DpnII GATC 1 cut(s) 546
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 20, 928
EagI CGGCCG 1 cut(s) 20
Eam1104I CTCTTC 4 cut(s) 75, 1571, 2272, 2677
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 4 cut(s) 75, 1571, 2272, 2677
EciI GGCGGA 1 cut(s) 709
Ecl136II GAGCTC 2 cut(s) 337, 766
EclXI CGGCCG 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 42, 1094, 2466
Eco24I GRGCYC 3 cut(s) 320, 339, 768
Eco31I GGTCTC 3 cut(s) 222, 733, 1380
Eco32I GATATC 2 cut(s) 2026, 2169
Eco47I GGWCC 6 cut(s) 327, 406, 810, 937, 964, 1922
Eco47III AGCGCT 1 cut(s) 1745
Eco52I CGGCCG 1 cut(s) 20
Eco53kI GAGCTC 2 cut(s) 337, 766
Eco72I CACGTG 1 cut(s) 645
Eco88I CYCGRG 3 cut(s) 313, 463, 1162
EcoICRI GAGCTC 2 cut(s) 337, 766
EcoRI GAATTC 1 cut(s) 1775
EcoRII CCWGG 4 cut(s) 597, 1007, 1756, 2141
EcoRV GATATC 2 cut(s) 2026, 2169
EcoT14I CCWWGG 3 cut(s) 42, 1094, 2466
EcoT38I GRGCYC 3 cut(s) 320, 339, 768
ErhI CCWWGG 3 cut(s) 42, 1094, 2466
FaeI CATG 9 cut(s) 46, 58, 1104, 1685, 1817, 1885, 1994, 2191, 2470
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 5 cut(s) 161, 351, 392, 1169, 2120
FatI CATG 9 cut(s) 42, 54, 1100, 1681, 1813, 1881, 1990, 2187, 2466
FauI CCCGC 5 cut(s) 46, 235, 535, 948, 2558
FauNDI CATATG 1 cut(s) 1301
FblI GTMKAC 1 cut(s) 2202
FriOI GRGCYC 3 cut(s) 320, 339, 768
FspBI CTAG 5 cut(s) 1095, 1140, 1497, 2096, 2678
GlaI GCGC 4 cut(s) 203, 1745, 2259, 2261
GsuI CTGGAG 1 cut(s) 2502
HaeII RGCGCY 1 cut(s) 1747
HaeIII GGCC 8 cut(s) 22, 438, 930, 1066, 1099, 1756, 2241, 2461
HgaI GACGC 3 cut(s) 253, 316, 885
HhaI GCGC 4 cut(s) 204, 1746, 2260, 2262
Hin1I GRCGYC 1 cut(s) 896
Hin1II CATG 9 cut(s) 46, 58, 1104, 1685, 1817, 1885, 1994, 2191, 2470
Hin6I GCGC 4 cut(s) 202, 1744, 2258, 2260
HinP1I GCGC 4 cut(s) 202, 1744, 2258, 2260
HincII GTYRAC 2 cut(s) 127, 1608
HindII GTYRAC 2 cut(s) 127, 1608
HphI GGTGA 8 cut(s) 6, 129, 448, 469, 767, 1097, 1558, 2176
Hpy166II GTNNAC 8 cut(s) 127, 909, 990, 1333, 1608, 1766, 1906, 2203
Hpy8I GTNNAC 8 cut(s) 127, 909, 990, 1333, 1608, 1766, 1906, 2203
Hpy99I CGWCG 7 cut(s) 31, 235, 241, 247, 310, 614, 901
HpyAV CCTTC 8 cut(s) 86, 104, 720, 832, 978, 1462, 1615, 2486
HpyCH4III ACNGT 6 cut(s) 261, 610, 755, 847, 2306, 2512
HpyCH4IV ACGT 5 cut(s) 417, 644, 686, 1016, 1912
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 31, 742, 2411, 2423
HpySE526I ACGT 5 cut(s) 417, 644, 686, 1016, 1912
Hsp92I GRCGYC 1 cut(s) 896
Hsp92II CATG 9 cut(s) 46, 58, 1104, 1685, 1817, 1885, 1994, 2191, 2470
HspAI GCGC 4 cut(s) 202, 1744, 2258, 2260
Kzo9I GATC 1 cut(s) 546
LguI GCTCTTC 1 cut(s) 2677
LmnI GCTCC 3 cut(s) 252, 696, 1694
Lsp1109I GCAGC 4 cut(s) 45, 216, 2401, 2616
LweI GCATC 4 cut(s) 794, 1362, 2372, 2434
MaeI CTAG 5 cut(s) 1095, 1140, 1497, 2096, 2678
MaeII ACGT 5 cut(s) 417, 644, 686, 1016, 1912
MaeIII GTNAC 6 cut(s) 135, 512, 1109, 2506, 2618, 2680
MalI GATC 1 cut(s) 548
MboI GATC 1 cut(s) 546
MfeI CAATTG 1 cut(s) 1512
MhlI GDGCHC 6 cut(s) 320, 339, 536, 768, 1897, 2141
MlyI GAGTC 5 cut(s) 185, 923, 1106, 1145, 2564
MmeI TCCRAC 4 cut(s) 915, 1124, 1231, 2469
MroXI GAANNNNTTC 2 cut(s) 1593, 1775
MseI TTAA 5 cut(s) 774, 1419, 1433, 1557, 1614
MslI CAYNNNNRTG 3 cut(s) 1105, 2161, 2471
MspA1I CMGCKG 2 cut(s) 922, 946
MunI CAATTG 1 cut(s) 1512
MvaI CCWGG 4 cut(s) 599, 1009, 1758, 2143
MvnI CGCG 7 cut(s) 202, 230, 305, 428, 542, 614, 2260
MwoI GCNNNNNNNGC 5 cut(s) 28, 31, 742, 2411, 2423
NciI CCSGG 6 cut(s) 434, 481, 654, 717, 952, 1178
NcoI CCATGG 2 cut(s) 42, 2466
NdeI CATATG 1 cut(s) 1301
NdeII GATC 1 cut(s) 546
NheI GCTAGC 1 cut(s) 1496
NlaIII CATG 9 cut(s) 46, 58, 1104, 1685, 1817, 1885, 1994, 2191, 2470
NmeAIII GCCGAG 3 cut(s) 318, 464, 2473
NmuCI GTSAC 4 cut(s) 135, 1109, 2506, 2680
NruI TCGCGA 1 cut(s) 428
NspI RCATGY 2 cut(s) 1817, 1885
OliI CACNNNNGTG 1 cut(s) 2161
PaeR7I CTCGAG 2 cut(s) 313, 463
PalAI GGCGCGCC 1 cut(s) 2258
PasI CCCWGGG 1 cut(s) 598
PauI GCGCGC 1 cut(s) 2258
PciI ACATGT 1 cut(s) 1813
PciSI GCTCTTC 1 cut(s) 2677
PcsI WCGNNNNNNNCGW 4 cut(s) 120, 219, 239, 893
PdmI GAANNNNTTC 2 cut(s) 1593, 1775
PfeI GAWTC 7 cut(s) 144, 149, 400, 506, 1721, 2579, 2651
PflMI CCANNNNNTGG 1 cut(s) 1763
PinAI ACCGGT 1 cut(s) 812
PleI GAGTC 5 cut(s) 185, 922, 1106, 1144, 2563
PmaCI CACGTG 1 cut(s) 645
PmlI CACGTG 1 cut(s) 645
PpsI GAGTC 5 cut(s) 185, 922, 1106, 1144, 2563
Ppu21I YACGTR 2 cut(s) 645, 687
PscI ACATGT 1 cut(s) 1813
PshAI GACNNNNGTC 3 cut(s) 416, 1290, 2112
PsiI TTATAA 1 cut(s) 1635
Psp124BI GAGCTC 2 cut(s) 339, 768
Psp6I CCWGG 4 cut(s) 597, 1007, 1756, 2141
PspCI CACGTG 1 cut(s) 645
PspGI CCWGG 4 cut(s) 597, 1007, 1756, 2141
PspPI GGNCC 8 cut(s) 327, 406, 436, 810, 937, 964, 1064, 1922
PspXI VCTCGAGB 1 cut(s) 463
PteI GCGCGC 1 cut(s) 2258
RruI TCGCGA 1 cut(s) 428
RsaI GTAC 8 cut(s) 225, 263, 377, 752, 781, 1318, 1907, 2103
RsaNI GTAC 8 cut(s) 224, 262, 376, 751, 780, 1317, 1906, 2102
RseI CAYNNNNRTG 3 cut(s) 1105, 2161, 2471
SacI GAGCTC 2 cut(s) 339, 768
SapI GCTCTTC 1 cut(s) 2677
SaqAI TTAA 5 cut(s) 774, 1419, 1433, 1557, 1614
Sau3AI GATC 1 cut(s) 546
Sau96I GGNCC 8 cut(s) 327, 406, 436, 810, 937, 964, 1064, 1922
SchI GAGTC 5 cut(s) 185, 923, 1106, 1145, 2564
SduI GDGCHC 6 cut(s) 320, 339, 536, 768, 1897, 2141
SfaNI GCATC 4 cut(s) 794, 1362, 2372, 2434
SfcI CTRYAG 2 cut(s) 1357, 2268
Sfr274I CTCGAG 2 cut(s) 313, 463
SgsI GGCGCGCC 1 cut(s) 2258
SinI GGWCC 6 cut(s) 327, 406, 810, 937, 964, 1922
SlaI CTCGAG 2 cut(s) 313, 463
SmiMI CAYNNNNRTG 3 cut(s) 1105, 2161, 2471
SmlI CTYRAG 2 cut(s) 313, 463
SmoI CTYRAG 2 cut(s) 313, 463
SspI AATATT 1 cut(s) 2395
SspMI CTAG 5 cut(s) 1095, 1140, 1497, 2096, 2678
SstI GAGCTC 2 cut(s) 339, 768
StyI CCWWGG 3 cut(s) 42, 1094, 2466
TaaI ACNGT 6 cut(s) 261, 610, 755, 847, 2306, 2512
TaiI ACGT 5 cut(s) 420, 647, 689, 1019, 1915
TaqI TCGA 9 cut(s) 147, 314, 464, 530, 561, 627, 876, 1382, 2636
TatI WGTACW 1 cut(s) 1316
TfiI GAWTC 7 cut(s) 144, 149, 400, 506, 1721, 2579, 2651
Tru1I TTAA 5 cut(s) 774, 1419, 1433, 1557, 1614
Tru9I TTAA 5 cut(s) 774, 1419, 1433, 1557, 1614
TscAI CASTG 7 cut(s) 916, 1654, 1743, 2068, 2433, 2547, 2721
TseFI GTSAC 4 cut(s) 135, 1109, 2506, 2680
TseI GCWGC 4 cut(s) 58, 204, 2414, 2604
Tsp45I GTSAC 4 cut(s) 135, 1109, 2506, 2680
TspDTI ATGAA 4 cut(s) 17, 21, 674, 2204
TspRI CASTG 7 cut(s) 916, 1654, 1743, 2068, 2433, 2547, 2721
Van91I CCANNNNNTGG 1 cut(s) 1763
VpaK11BI GGWCC 6 cut(s) 327, 406, 810, 937, 964, 1922
XapI RAATTY 7 cut(s) 9, 253, 1384, 1393, 1775, 1852, 1927
XceI RCATGY 2 cut(s) 1817, 1885
XcmI CCANNNNNNNNNTGG 4 cut(s) 376, 1932, 2149, 2270
XhoI CTCGAG 2 cut(s) 313, 463
XmaJI CCTAGG 1 cut(s) 1094
XmiI GTMKAC 1 cut(s) 2202
XmnI GAANNNNTTC 2 cut(s) 1593, 1775
XspI CTAG 5 cut(s) 1095, 1140, 1497, 2096, 2678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.