pycom01g09580

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011114
Physical Location & Seq
Reverse (-)
10427303 .. 10430053
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09580.1

Sequence Viewer

Length: 2751 bp
ATGAATGAAATTTCACCACGGCCGCCGTCGCCGCCACCCGCCATGGCTTCTTCCATGCTGCCCATTTCTCTTCTGCTTCTTCTACTCCAAGCCTTTCTTCTAAAACCCACAAACGCCATCCCCATTGACGAATTGTCACCCCGAATCGAATCCCAGTTCTACACCAGAGCCAAACTCCCCGACTTTCCTGACTCCCCCACGCGCAGCACAAACGGGTCTCCCGTACCCGCGACGGCGACGGCGACGGCGAAGCTCCCGAATTTACAGTACCCAATGGCGAGGAACAGCGAAGTTGTAATCTTTGGGAACGCGACGCTCTCGAGCCCCAACCGGACCTTTGCACTCGGCTTCTTCTCCACCAACGACGAAAGTCCCAAATGGTACTTGGGCATTTGGTATGCTCTGATTCCGGTCCCGACTTACGTCTGGGTCGCGAACCGGGACCGACCCGTCAATAATCTCACCTCCTCGAGCTTGGAAATCACCGGGTCGGGTCGGTTGGTGGTCAAAGAATCCGGTAACTCAATCGTGTGGTCGAGCACGAACGCGGGGATCGGCGTTAGCGTCAAGCTTTTGGAGAATGGCAATCTGGTTTTGCTTACCCAGGGAGGCACCGTCGCGTGGCAGAGCTTCGATTACCCGACTGACACGTGGCTTCCGGGGATGAATCTGACGAGCGAGCGAGGGCTTACGTGTTGGCGGAGCTACATAGACCCTGCGCCGGGTTTGTATTCTTTGCGGCTGAGACCGCCGGAGTACGGTGAGTTTGAGCTCGTTTTTAATGGTACTGCAACTTACTGGACAACTGGGAATTGGACCGGTAATGCCTTCGCAAATGTGCCTCAAATGACGGTGCCTTACATATATAAGTTCCATTTTATCGACCCATTCACGCCAACGGCGTCGTTCGGGTTCACTGAGTCGCCGCTGGATGGCCGGTTGGACCTGCCTCTGACCCGGTTCCAGATGGACCCATCGGGTCAGCTCAGGCAGTTCACTTGGGCAACCCAGACCTGGAACACGTTTTGGTTTCAGCCTGAGAAAAAGTGCAGAGTGTTTGGTATGTGTGGGCCTTTTGGGGTTTGCAATGGTGAAACACCTAGGCCATGTGAGTGTGACTCCGGTTTTCAGCCGGTGGATGAGTCTAGTTGGAACTTAGGGGACTACTCGGGGGGTTGCCGCCGGGTGTTTAATGAGGGAGTGGCTTGTGGTCAGAGGGATGATAGGTTTGACAAGGTTGGTGTTGTGAGATATGTTGGAAGTTATAGTAAGCCTTATAGAATTAACTTTGACGATTGTCAGCACACATGCTTGCTTTCATGTACTTGCATAGGTGTGGACTACGATAACATTACTGGCGTGTGTAGAGTGTATTATGGGTCTCTCTCGAATTTGCAAAATTTGAGCTCCGAAAGTGGATTTGCTTATGATTTCTATTTAAGGGTGCAAAAAAGTGCGACAAGATTGGAGAAGAAGGTGCTAAATCGTGTTGTTTTGAGTGCTAGCATTGCTGGGTCAATTGTAATTTTGGGCTTTGTAGCGGCGGTGGTGGTGATTTTTATTAAGGTACGAGAGAAGAAGAAGAAGAAGAGAGAAACAGAAAAGGTTCAGCTTATGTCAACCTTTAATTTGAAGGTATTTTCTTATAAAGAACTTGACACTGCAACACGGGGTTTTTCAGACAAACTTGGGCATGGTGGATTTGGAGCTGTTTTCCACGGGGAGTTATCAGATTCTACTATTGTTGCAGTGAAGCGCTTAGACAGGCCAGGTGGTGGACAGAAAGAATTCCAAGCGGAAGTTTGCACGATTGGGAACATTCAACATGTCAATCTTGTCAGACTTAGAGGCTTCTGCTCAGAAAATTCCCATAGACTTTTGGTTTATGATTACATGCCAAATGGTGCTCTTAGCGTGTACCTACGTCAAGATGGTCCAAATTTGAGTTGGGATGTTAGATTTCGGATAGCAGTTGGTATGGCAAGAGGCATTGCATATTTACATGAGGAGTGTAGAGAGTGCATAATCCATTGTGATATCAAACCAGAAAACATTTTGCTTGATAGTTCTTGCACTGCAAAGGTGTCAGATTTTGGGTTGGCAAAGCTAGTTGTACGGGACTTTAGTCGGGTATTAGCCACAATGAGGGGCACCAGGGGCTATATGGCGCCAGAGTGGATATCTGGTGTGGCAATCACCACGAAAGCTGATGTATACAGCTATGGAATGACACTAATAGAATTACTTGCAGGCCGCAGAAATGTGGAGGTGCGCCAATCTGTAGATGGAAGAGAGGGTGCAAGTGAAACAACTGACAGTTTGTTCTTTCCTCCACGGGCGGCACAGAAAATAATTGAGGGCAATGTAAGGGAAGTTATTGACGATAGGCTTGGATGCACATACAATATTGAGGAGGCAAAGCGTGTTGCAATGGTGGCAGTGTGGTGCATACAGGATGCCGAGGCAATGCGGCCTACAATGGGGATGGTAGTGAAAATGTTGGAAGGGCTGGTGGAAGTGACTGTTCCTCCAGCACCCAAATTGTTGCAAGCACTGGTTTCGGGGGAGTCTTTTTGCGGGGTAAAGGCTGATTCTGGAATTGGGTTATCTAATGGCAGCGACTTCTCCGGTTACAATACAAGATTATCTAGTGGTTGTTCAGAATCGTCTATTGGTAATGGCTCTTCCCTAGTGACTGAGATTGTGGATGATAACCAAAGAAGTTCAGTGGGCTTTACTGACCAAGGCTAA

Protein Analysis

917

Amino Acids

101.07

Weight (kDa)

6.74

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 140 - 227 3.9e-26 D-mannose binding lectin
S_locus_glycop PF00954 267 - 378 1.2e-21 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 560 - 832 5.4e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 561 - 760 1.4e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1647
AasI GACNNNNNNGTC 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 954
AccB1I GGYRCC 4 cut(s) 611, 853, 2150, 2167
AccB7I CCANNNNNTGG 1 cut(s) 1775
AccI GTMKAC 1 cut(s) 2214
AccII CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 2 cut(s) 20, 934
AcsI RAATTY 7 cut(s) 9, 259, 1390, 1399, 1787, 1864, 1939
AcvI CACGTG 1 cut(s) 651
AcyI GRCGYC 2 cut(s) 902, 2168
AfaI GTAC 9 cut(s) 225, 269, 383, 758, 787, 1324, 1569, 1919, 2115
AfeI AGCGCT 1 cut(s) 1757
AfiI CCNNNNNNNGG 9 cut(s) 621, 721, 722, 758, 932, 1014, 1775, 2145, 2480
AflIII ACRYGT 4 cut(s) 648, 692, 1020, 1825
AgeI ACCGGT 1 cut(s) 818
AgsI TTSAA 2 cut(s) 1633, 1823
AhdI GACNNNNNGTC 1 cut(s) 133
AjnI CCWGG 4 cut(s) 603, 1013, 1768, 2153
AjuI GAANNNNNNNTTGG 4 cut(s) 81, 113, 2655, 2687
Alw21I GWGCWC 4 cut(s) 542, 774, 1409, 1909
Alw26I GTCTC 3 cut(s) 222, 739, 1386
AlwI GGATC 1 cut(s) 560
Ama87I CYCGRG 3 cut(s) 319, 469, 1168
Aor51HI AGCGCT 1 cut(s) 1757
AoxI GGCC 7 cut(s) 20, 934, 1070, 1103, 1766, 2251, 2471
ApeKI GCWGC 3 cut(s) 58, 204, 2616
ApoI RAATTY 7 cut(s) 9, 259, 1390, 1399, 1787, 1864, 1939
AsiGI ACCGGT 1 cut(s) 818
Asp700I GAANNNNTTC 2 cut(s) 1605, 1787
AspA2I CCTAGG 1 cut(s) 1100
AspLEI GCGC 5 cut(s) 204, 721, 1758, 2170, 2274
AspS9I GGNCC 8 cut(s) 333, 412, 442, 816, 943, 970, 1070, 1934
AsuC2I CCSGG 6 cut(s) 440, 487, 660, 723, 958, 1184
AsuHPI GGTGA 8 cut(s) 6, 129, 454, 475, 773, 1103, 1564, 2188
AsuNHI GCTAGC 1 cut(s) 1502
AvaI CYCGRG 3 cut(s) 319, 469, 1168
AvaII GGWCC 7 cut(s) 333, 412, 442, 816, 943, 970, 1934
AvrII CCTAGG 1 cut(s) 1100
BaeGI GKGCMC 1 cut(s) 2153
BanI GGYRCC 4 cut(s) 611, 853, 2150, 2167
BanII GRGCYC 3 cut(s) 326, 774, 1409
BarI GAAGNNNNNNTAC 2 cut(s) 2668, 2700
BbrPI CACGTG 1 cut(s) 651
Bbv12I GWGCWC 4 cut(s) 542, 774, 1409, 1909
BbvI GCAGC 3 cut(s) 45, 216, 2628
BccI CCATC 7 cut(s) 125, 926, 961, 982, 1925, 2279, 2479
BceAI ACGGC 6 cut(s) 10, 35, 249, 255, 261, 915
BcgI CGANNNNNNTGC 2 cut(s) 2541, 2575
BciT130I CCWGG 4 cut(s) 605, 1015, 1770, 2155
BcnI CCSGG 6 cut(s) 440, 487, 660, 723, 958, 1184
BcoDI GTCTC 3 cut(s) 222, 739, 1386
BfaI CTAG 6 cut(s) 1101, 1146, 1503, 2108, 2649, 2690
BfmI CTRYAG 1 cut(s) 2280
BfoI RGCGCY 2 cut(s) 1759, 2171
BfuAI ACCTGC 1 cut(s) 954
BlnI CCTAGG 1 cut(s) 1100
Bme18I GGWCC 7 cut(s) 333, 412, 442, 816, 943, 970, 1934
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 3 cut(s) 319, 469, 1168
BmgT120I GGNCC 8 cut(s) 333, 412, 442, 816, 943, 970, 1070, 1934
BmiI GGNNCC 8 cut(s) 414, 443, 613, 855, 962, 972, 2152, 2169
BmrI ACTGGG 2 cut(s) 148, 816
BmsI GCATC 2 cut(s) 2384, 2446
BmtI GCTAGC 1 cut(s) 1506
BmuI ACTGGG 2 cut(s) 148, 816
BoxI GACNNNNGTC 4 cut(s) 369, 422, 1296, 2124
BplI GAGNNNNNCTC 4 cut(s) 159, 191, 1103, 1135
BpmI CTGGAG 1 cut(s) 2514
Bpu10I CCTNAGC 1 cut(s) 986
BpuMI CCSGG 6 cut(s) 440, 487, 660, 723, 958, 1184
BsaAI YACGTR 2 cut(s) 651, 693
BsaHI GRCGYC 2 cut(s) 902, 2168
BsaI GGTCTC 3 cut(s) 222, 739, 1386
BsaWI WCCGGW 6 cut(s) 330, 409, 515, 818, 1121, 2627
BsaXI ACNNNNNCTCC 4 cut(s) 600, 630, 2512, 2542
Bsc4I CCNNNNNNNGG 9 cut(s) 621, 721, 722, 758, 932, 1014, 1775, 2145, 2480
Bse118I RCCGGY 3 cut(s) 818, 936, 1132
Bse1I ACTGG 5 cut(s) 154, 803, 811, 1360, 2559
Bse3DI GCAATG 6 cut(s) 1093, 1506, 1989, 2368, 2436, 2472
BseBI CCWGG 4 cut(s) 605, 1015, 1770, 2155
BseLI CCNNNNNNNGG 9 cut(s) 621, 721, 722, 758, 932, 1014, 1775, 2145, 2480
BseMI GCAATG 6 cut(s) 1093, 1506, 1989, 2368, 2436, 2472
BseMII CTCAG 6 cut(s) 734, 909, 1000, 1029, 1872, 2688
BseNI ACTGG 5 cut(s) 154, 803, 811, 1360, 2559
BseRI GAGGAG 3 cut(s) 457, 2021, 2426
BseSI GKGCMC 1 cut(s) 2153
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 45, 216, 2628
BseYI CCCAGC 1 cut(s) 1511
BsgI GTGCAG 1 cut(s) 1069
Bsh1236I CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 7 cut(s) 22, 936, 1072, 1105, 1768, 2253, 2473
BshNI GGYRCC 4 cut(s) 611, 853, 2150, 2167
BshTI ACCGGT 1 cut(s) 818
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 4 cut(s) 542, 774, 1409, 1909
BsiHKCI CYCGRG 3 cut(s) 319, 469, 1168
BslFI GGGAC 5 cut(s) 357, 398, 455, 1175, 2132
BslI CCNNNNNNNGG 9 cut(s) 621, 721, 722, 758, 932, 1014, 1775, 2145, 2480
BsmAI GTCTC 3 cut(s) 222, 739, 1386
BsmFI GGGAC 5 cut(s) 357, 398, 455, 1175, 2132
BsnI GGCC 7 cut(s) 22, 936, 1072, 1105, 1768, 2253, 2473
Bso31I GGTCTC 3 cut(s) 222, 739, 1386
BsoBI CYCGRG 3 cut(s) 319, 469, 1168
Bsp1286I GDGCHC 6 cut(s) 326, 542, 774, 1409, 1909, 2153
Bsp143I GATC 1 cut(s) 552
Bsp19I CCATGG 1 cut(s) 42
Bsp68I TCGCGA 1 cut(s) 434
BspANI GGCC 7 cut(s) 22, 936, 1072, 1105, 1768, 2253, 2473
BspCNI CTCAG 6 cut(s) 735, 910, 999, 1030, 1871, 2689
BspFNI CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
BspLI GGNNCC 8 cut(s) 414, 443, 613, 855, 962, 972, 2152, 2169
BspMI ACCTGC 1 cut(s) 954
BspOI GCTAGC 1 cut(s) 1506
BspPI GGATC 1 cut(s) 560
BspQI GCTCTTC 1 cut(s) 2689
BspT107I GGYRCC 4 cut(s) 611, 853, 2150, 2167
BspTNI GGTCTC 3 cut(s) 222, 739, 1386
BsrDI GCAATG 6 cut(s) 1093, 1506, 1989, 2368, 2436, 2472
BsrFI RCCGGY 3 cut(s) 818, 936, 1132
BsrI ACTGG 5 cut(s) 154, 803, 811, 1360, 2559
BssAI RCCGGY 3 cut(s) 818, 936, 1132
BssMI GATC 1 cut(s) 552
BssNAI GTATAC 1 cut(s) 2215
BssNI GRCGYC 2 cut(s) 902, 2168
BssT1I CCWWGG 3 cut(s) 42, 1100, 2743
Bst1107I GTATAC 1 cut(s) 2215
Bst2UI CCWGG 4 cut(s) 605, 1015, 1770, 2155
Bst4CI ACNGT 6 cut(s) 267, 616, 761, 853, 2318, 2524
Bst6I CTCTTC 4 cut(s) 75, 1583, 2284, 2689
BstACI GRCGYC 2 cut(s) 902, 2168
BstBAI YACGTR 2 cut(s) 651, 693
BstC8I GCNNGC 5 cut(s) 680, 1313, 1504, 2251, 2550
BstDSI CCRYGG 4 cut(s) 17, 42, 1717, 2333
BstFNI CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
BstH2I RGCGCY 2 cut(s) 1759, 2171
BstHHI GCGC 5 cut(s) 204, 721, 1758, 2170, 2274
BstKTI GATC 1 cut(s) 555
BstMAI GTCTC 3 cut(s) 222, 739, 1386
BstMBI GATC 1 cut(s) 552
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 6 cut(s) 28, 31, 748, 1508, 2157, 2435
BstNI CCWGG 4 cut(s) 605, 1015, 1770, 2155
BstNSI RCATGY 3 cut(s) 1311, 1829, 1897
BstPAI GACNNNNGTC 4 cut(s) 369, 422, 1296, 2124
BstSFI CTRYAG 1 cut(s) 2280
BstSLI GKGCMC 1 cut(s) 2153
BstUI CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
BstV1I GCAGC 3 cut(s) 45, 216, 2628
BstZ17I GTATAC 1 cut(s) 2215
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 7 cut(s) 22, 936, 1072, 1105, 1768, 2253, 2473
BtgI CCRYGG 4 cut(s) 17, 42, 1717, 2333
BtsI GCAGTG 4 cut(s) 1659, 1755, 2073, 2445
BtsIMutI CAGTG 7 cut(s) 915, 1659, 1755, 2073, 2445, 2552, 2733
BtuMI TCGCGA 1 cut(s) 434
BveI ACCTGC 1 cut(s) 954
Cac8I GCNNGC 5 cut(s) 680, 1313, 1504, 2251, 2550
CfoI GCGC 5 cut(s) 204, 721, 1758, 2170, 2274
Cfr10I RCCGGY 3 cut(s) 818, 936, 1132
Cfr13I GGNCC 8 cut(s) 333, 412, 442, 816, 943, 970, 1070, 1934
CseI GACGC 3 cut(s) 322, 553, 891
Csp6I GTAC 9 cut(s) 224, 268, 382, 757, 786, 1323, 1568, 1918, 2114
CspAI ACCGGT 1 cut(s) 818
CviAII CATG 9 cut(s) 43, 55, 1107, 1308, 1320, 1694, 1826, 1894, 2003
CviQI GTAC 9 cut(s) 224, 268, 382, 757, 786, 1323, 1568, 1918, 2114
DinI GGCGCC 1 cut(s) 2169
DpnI GATC 1 cut(s) 554
DpnII GATC 1 cut(s) 552
DrdI GACNNNNNNGTC 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 2 cut(s) 20, 934
EagI CGGCCG 1 cut(s) 20
Eam1104I CTCTTC 4 cut(s) 75, 1583, 2284, 2689
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 4 cut(s) 75, 1583, 2284, 2689
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 2 cut(s) 772, 1407
EclXI CGGCCG 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 42, 1100, 2743
Eco24I GRGCYC 3 cut(s) 326, 774, 1409
Eco31I GGTCTC 3 cut(s) 222, 739, 1386
Eco32I GATATC 2 cut(s) 2038, 2181
Eco47I GGWCC 7 cut(s) 333, 412, 442, 816, 943, 970, 1934
Eco47III AGCGCT 1 cut(s) 1757
Eco52I CGGCCG 1 cut(s) 20
Eco53kI GAGCTC 2 cut(s) 772, 1407
Eco72I CACGTG 1 cut(s) 651
Eco88I CYCGRG 3 cut(s) 319, 469, 1168
EcoICRI GAGCTC 2 cut(s) 772, 1407
EcoRI GAATTC 1 cut(s) 1787
EcoRII CCWGG 4 cut(s) 603, 1013, 1768, 2153
EcoRV GATATC 2 cut(s) 2038, 2181
EcoT14I CCWWGG 3 cut(s) 42, 1100, 2743
EcoT38I GRGCYC 3 cut(s) 326, 774, 1409
EgeI GGCGCC 1 cut(s) 2169
EheI GGCGCC 1 cut(s) 2169
ErhI CCWWGG 3 cut(s) 42, 1100, 2743
FaeI CATG 9 cut(s) 46, 58, 1110, 1311, 1323, 1697, 1829, 1897, 2006
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 5 cut(s) 357, 398, 455, 1175, 2132
FatI CATG 9 cut(s) 42, 54, 1106, 1307, 1319, 1693, 1825, 1893, 2002
FauI CCCGC 4 cut(s) 46, 235, 541, 2570
FblI GTMKAC 1 cut(s) 2214
FriOI GRGCYC 3 cut(s) 326, 774, 1409
FspBI CTAG 6 cut(s) 1101, 1146, 1503, 2108, 2649, 2690
GlaI GCGC 5 cut(s) 203, 720, 1757, 2169, 2273
GsaI CCCAGC 1 cut(s) 1515
GsuI CTGGAG 1 cut(s) 2514
HaeII RGCGCY 2 cut(s) 1759, 2171
HaeIII GGCC 7 cut(s) 22, 936, 1072, 1105, 1768, 2253, 2473
HgaI GACGC 3 cut(s) 322, 553, 891
HhaI GCGC 5 cut(s) 204, 721, 1758, 2170, 2274
Hin1I GRCGYC 2 cut(s) 902, 2168
Hin1II CATG 9 cut(s) 46, 58, 1110, 1311, 1323, 1697, 1829, 1897, 2006
Hin6I GCGC 5 cut(s) 202, 719, 1756, 2168, 2272
HinP1I GCGC 5 cut(s) 202, 719, 1756, 2168, 2272
HincII GTYRAC 1 cut(s) 1620
HindII GTYRAC 1 cut(s) 1620
HindIII AAGCTT 1 cut(s) 569
HphI GGTGA 8 cut(s) 6, 129, 454, 475, 773, 1103, 1564, 2188
Hpy166II GTNNAC 7 cut(s) 915, 996, 1339, 1620, 1778, 1918, 2215
Hpy188III TCNNGA 9 cut(s) 188, 256, 319, 415, 433, 964, 1387, 1928, 2595
Hpy8I GTNNAC 7 cut(s) 915, 996, 1339, 1620, 1778, 1918, 2215
Hpy99I CGWCG 8 cut(s) 31, 235, 241, 247, 316, 368, 620, 907
HpyAV CCTTC 4 cut(s) 838, 1468, 1627, 2498
HpyCH4III ACNGT 6 cut(s) 267, 616, 761, 853, 2318, 2524
HpyCH4IV ACGT 5 cut(s) 423, 650, 692, 1022, 1924
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 31, 748, 1508, 2157, 2435
HpySE526I ACGT 5 cut(s) 423, 650, 692, 1022, 1924
Hsp92I GRCGYC 2 cut(s) 902, 2168
Hsp92II CATG 9 cut(s) 46, 58, 1110, 1311, 1323, 1697, 1829, 1897, 2006
HspAI GCGC 5 cut(s) 202, 719, 1756, 2168, 2272
KasI GGCGCC 1 cut(s) 2167
Kzo9I GATC 1 cut(s) 552
LguI GCTCTTC 1 cut(s) 2689
LmnI GCTCC 4 cut(s) 258, 702, 1412, 1706
Lsp1109I GCAGC 3 cut(s) 45, 216, 2628
LweI GCATC 2 cut(s) 2384, 2446
MaeI CTAG 6 cut(s) 1101, 1146, 1503, 2108, 2649, 2690
MaeII ACGT 5 cut(s) 423, 650, 692, 1022, 1924
MaeIII GTNAC 6 cut(s) 135, 518, 1115, 2518, 2630, 2692
MalI GATC 1 cut(s) 554
MboI GATC 1 cut(s) 552
MfeI CAATTG 1 cut(s) 1518
MhlI GDGCHC 6 cut(s) 326, 542, 774, 1409, 1909, 2153
Mly113I GGCGCC 1 cut(s) 2168
MlyI GAGTC 5 cut(s) 185, 929, 1112, 1151, 2576
MmeI TCCRAC 4 cut(s) 921, 1130, 1237, 2481
MroXI GAANNNNTTC 2 cut(s) 1605, 1787
MseI TTAA 6 cut(s) 780, 1191, 1284, 1439, 1563, 1626
MslI CAYNNNNRTG 2 cut(s) 1111, 1334
MspA1I CMGCKG 1 cut(s) 928
MunI CAATTG 1 cut(s) 1518
MvaI CCWGG 4 cut(s) 605, 1015, 1770, 2155
MvnI CGCG 6 cut(s) 202, 230, 311, 434, 548, 620
MwoI GCNNNNNNNGC 6 cut(s) 28, 31, 748, 1508, 2157, 2435
NarI GGCGCC 1 cut(s) 2168
NciI CCSGG 6 cut(s) 440, 487, 660, 723, 958, 1184
NcoI CCATGG 1 cut(s) 42
NdeII GATC 1 cut(s) 552
NheI GCTAGC 1 cut(s) 1502
NlaIII CATG 9 cut(s) 46, 58, 1110, 1311, 1323, 1697, 1829, 1897, 2006
NlaIV GGNNCC 8 cut(s) 414, 443, 613, 855, 962, 972, 2152, 2169
NmeAIII GCCGAG 2 cut(s) 324, 2485
NmuCI GTSAC 4 cut(s) 135, 1115, 2518, 2692
NruI TCGCGA 1 cut(s) 434
NspI RCATGY 3 cut(s) 1311, 1829, 1897
PaeR7I CTCGAG 2 cut(s) 319, 469
PasI CCCWGGG 1 cut(s) 604
PciI ACATGT 1 cut(s) 1825
PciSI GCTCTTC 1 cut(s) 2689
PcsI WCGNNNNNNNCGW 5 cut(s) 219, 239, 245, 561, 899
PdmI GAANNNNTTC 2 cut(s) 1605, 1787
PfeI GAWTC 8 cut(s) 144, 149, 406, 512, 667, 1733, 2591, 2663
PflMI CCANNNNNTGG 1 cut(s) 1775
PinAI ACCGGT 1 cut(s) 818
PleI GAGTC 5 cut(s) 185, 928, 1112, 1150, 2575
PluTI GGCGCC 1 cut(s) 2171
PmaCI CACGTG 1 cut(s) 651
PmlI CACGTG 1 cut(s) 651
PpsI GAGTC 5 cut(s) 185, 928, 1112, 1150, 2575
Ppu21I YACGTR 2 cut(s) 651, 693
PscI ACATGT 1 cut(s) 1825
PshAI GACNNNNGTC 4 cut(s) 369, 422, 1296, 2124
PsiI TTATAA 1 cut(s) 1647
Psp124BI GAGCTC 2 cut(s) 774, 1409
Psp6I CCWGG 4 cut(s) 603, 1013, 1768, 2153
PspCI CACGTG 1 cut(s) 651
PspFI CCCAGC 1 cut(s) 1511
PspGI CCWGG 4 cut(s) 603, 1013, 1768, 2153
PspN4I GGNNCC 8 cut(s) 414, 443, 613, 855, 962, 972, 2152, 2169
PspPI GGNCC 8 cut(s) 333, 412, 442, 816, 943, 970, 1070, 1934
PspXI VCTCGAGB 1 cut(s) 469
RruI TCGCGA 1 cut(s) 434
RsaI GTAC 9 cut(s) 225, 269, 383, 758, 787, 1324, 1569, 1919, 2115
RsaNI GTAC 9 cut(s) 224, 268, 382, 757, 786, 1323, 1568, 1918, 2114
RseI CAYNNNNRTG 2 cut(s) 1111, 1334
SacI GAGCTC 2 cut(s) 774, 1409
SapI GCTCTTC 1 cut(s) 2689
SaqAI TTAA 6 cut(s) 780, 1191, 1284, 1439, 1563, 1626
Sau3AI GATC 1 cut(s) 552
Sau96I GGNCC 8 cut(s) 333, 412, 442, 816, 943, 970, 1070, 1934
SchI GAGTC 5 cut(s) 185, 929, 1112, 1151, 2576
SduI GDGCHC 6 cut(s) 326, 542, 774, 1409, 1909, 2153
SfaNI GCATC 2 cut(s) 2384, 2446
SfcI CTRYAG 1 cut(s) 2280
SfoI GGCGCC 1 cut(s) 2169
Sfr274I CTCGAG 2 cut(s) 319, 469
SinI GGWCC 7 cut(s) 333, 412, 442, 816, 943, 970, 1934
SlaI CTCGAG 2 cut(s) 319, 469
SmiMI CAYNNNNRTG 2 cut(s) 1111, 1334
SmlI CTYRAG 2 cut(s) 319, 469
SmoI CTYRAG 2 cut(s) 319, 469
SspDI GGCGCC 1 cut(s) 2167
SspI AATATT 1 cut(s) 2407
SspMI CTAG 6 cut(s) 1101, 1146, 1503, 2108, 2649, 2690
SstI GAGCTC 2 cut(s) 774, 1409
StyI CCWWGG 3 cut(s) 42, 1100, 2743
TaaI ACNGT 6 cut(s) 267, 616, 761, 853, 2318, 2524
TaiI ACGT 5 cut(s) 426, 653, 695, 1025, 1927
TaqI TCGA 7 cut(s) 147, 320, 470, 536, 633, 882, 1388
TaqII GACCGA 1 cut(s) 459
TatI WGTACW 1 cut(s) 1322
TauI GCSGC 9 cut(s) 25, 34, 742, 928, 1182, 1544, 2256, 2342, 2473
TfiI GAWTC 8 cut(s) 144, 149, 406, 512, 667, 1733, 2591, 2663
Tru1I TTAA 6 cut(s) 780, 1191, 1284, 1439, 1563, 1626
Tru9I TTAA 6 cut(s) 780, 1191, 1284, 1439, 1563, 1626
TscAI CASTG 7 cut(s) 922, 1666, 1755, 2080, 2445, 2559, 2733
TseFI GTSAC 4 cut(s) 135, 1115, 2518, 2692
TseI GCWGC 3 cut(s) 58, 204, 2616
Tsp45I GTSAC 4 cut(s) 135, 1115, 2518, 2692
TspDTI ATGAA 4 cut(s) 17, 21, 680, 1308
TspRI CASTG 7 cut(s) 922, 1666, 1755, 2080, 2445, 2559, 2733
Van91I CCANNNNNTGG 1 cut(s) 1775
VpaK11BI GGWCC 7 cut(s) 333, 412, 442, 816, 943, 970, 1934
XapI RAATTY 7 cut(s) 9, 259, 1390, 1399, 1787, 1864, 1939
XceI RCATGY 3 cut(s) 1311, 1829, 1897
XcmI CCANNNNNNNNNTGG 4 cut(s) 382, 1944, 2161, 2282
XhoI CTCGAG 2 cut(s) 319, 469
XmaJI CCTAGG 1 cut(s) 1100
XmiI GTMKAC 1 cut(s) 2214
XmnI GAANNNNTTC 2 cut(s) 1605, 1787
XspI CTAG 6 cut(s) 1101, 1146, 1503, 2108, 2649, 2690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.