Rroxscaffold_4G00299390

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011114
Physical Location & Seq
Forward (+)
19321105 .. 19326572
5468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299390.1

Sequence Viewer

Length: 3558 bp
ATGCCTTCTTCCATGGTTCCCGTCTCTCTTCTCCTCCCCATACTCCAAACCCTAATTCTCATCTCCACAAACTCCAATCCCATTCCCAAAACTACTTCACCCCAAAACCCAACCCAGTTCTCCAACACCCTTCCACGTCCCACTCCAAACTCAAACGGGTCTTCAAGAACTGTCTCGAAGATTCTTTGGTACACACCCTTGAGAAGCAACAAGGTTATACTCTATGGCAACTCCACCATTTGCAGTCGCAACAGGACCTTTCAGCTCGGCTTCTTCCCCTCCAATGGCGGCGCCTACTTGGCAATAATGTACTCCTCAATTCAGGTCCCCACCGTAGTCTGGGTCGCCAACCGCGACCGCCCCGTCAAGAATCTCACTTCAGCCAGACTCGAAATCACAGCCACCGACGGCCGGCTCGTTGTTATAGACTCCGATAACTCGACTCTCTGGGCCAGCCAGAACGCCGCCGCCGCCGTCGGCGCCCAAGTCGTGCTGTTGGAGAGCGGCAATCTGGTGCTGCTCACCCACTCCGGGCAACTCGCGTGGCAGAGCTTCGATCACCCGGCTGACACGTGGCTCCCCGGCATGAATCTGACGGCGGAGCGGGGCCTGACCAGCTGGCGGAGCGCGGAGGACCCGTCCCCGGGTTTGTATTCCCTGCGGTTGCGCCCACGGGATTACGGTGAGTTTGAGCTAGTTTTTAACGGTAGTGTTGAGTACTGGAGGACCGGGGATTGGGACGGTCACGCTTTCGCTAATGTGCCGGAGATGATTGTTAAGTATATCTATACGTTTCACTTCATAGACCCGTACAAGCCGACGGCGTCGTTTTGGTTCACCGAGGGGCCGTTGGAGAGCGGGTTGGACCCGCCGCTGACCCGGTTCCAGATAGACCATTCGGGTCAGCTCCGGCAGTATACTTGGGGTTTAAGCGAGACATGGGACAAGTTTTGGTACCAGCCTGAGGACAAGTGCAGAGTGTTTGGTTTGTGTGGGAGGTTTGGGGTTTGTGATAGTGAGACTGCCGAACCCTGTGAGTGTCCCTCAGGGTTTAAGCCTGTGGATGAGTCTGGTTGGGACTCTGGGGACTATTCAGGTGGGTGTGAGAGGTTTGGTGATGTGTCTTGTGAAGAAAGAGATGATGGTTTCGTGGAGGTTTGGGTTGAGAGTTTTGCAGACAGTAGGCATAATAGTTCGATTACTGATAGGGGTGGTTGTGAAAAGATGTGCTTGCAAGTATGTTCTTGTATTGGTTTTCAGTACAACGAGAAGTCTGGTTTGTGTAGACATTATTTCGGGTCTCTGTCGAATTTGAGGAATGTGAGCTCTGATGGTAGTAGCAGTATGGATGCTGGTAGTGTTTTGAATTTGAAGGTACGAAAAGGTGTGATCTTGAATAACAGGAAGGCTAAGGTTATGAATTATACAGTTTTCGGTGCTAGCATTGTTGGGTCAGTTACGGTTTTGGGTTTTGTGGTGGTGGTTGTATTGATTAGGAGAAGAAGGATGAAGAGGAATGGAGGAGCAGAGGAAAGTGTTTTGCTGCCGGTGTTGAATTTGAAGGTATTTTCCTACAAAGAGCTTCAGACTGCAACCGGGGGTTTTAAAGAGAAACTCGGGCATGGTGGCTTTGGAGCGGTTTTCCTAGGGGAGCTATCAGACCGTACTCTTGTTGCAGTGAAACGCTTGGAGAGGCCAGGCAGCGGAGAGCAACAATTCCGAGCAGAAGTTTGCACCATTGGCAACATTCAGCATGTCAATCTTGTGAGACTTAGAGGCTTCTGCTCAGAAGATTCTCATAGACTTCTGGTATATGATTACATGCCAAATGGTCCTCTAAGTGTGTATCTACGTCGGGACGGTCCAAATTTGAACTGGGATGTTAGGTTTCGGCTAGCGGTTGGTACGGCAAGAGGCATTGCATATTTACATGAGGGGTGTAGAGATTCCATAATCCATTGTGATATCAAACCAGAAAACATTTTACTTGATAGTGATTACACGGCAAAGGTGTCAGATTTTGGGTTAGCAAAGCTAATTCACAGAGACTTTAGTGGAGCATTAGCTGCTGATGGTAATCATGGCTATACCTATGGCTATGTAGCGCCAGAGTGCAGACCTGGTGATCCAATCACCACAAAAGCCGATGTTTACAGCTATGGAAAGACATTGATAGAATTACTTGGAGGCCATAGAATGTGGGAGGCACCTCCATCTGTAGGTGGCAGAGAGGGTGGAAATGAAATAACAGATTCTTGGTGCTTTCCTTCGTGGGCGGCACAGCAGATAATTGATGGCAATGTGGTGGCTGTAATTGATGATAGACTCGGGAGCAAGTATAACCTTAAGGAGGCAGAGCGTGTTGCATTAGTGGCGGTGTGGTGCATACAGGACGACGAGGCAACAAGGCCTACAATGGGGATGGTAGTGACAATGTTGGAAGGGGTTGTGGAAGTGACTGTTCCTCCAGCACCAAAGTTGTTACAAGCGCTGGTTTCAGGGGAGTCATTCTGTGGGGTGAATTCCGATTCTGGACACAGTCCATCGATTGCCAGCGACAACTTCTCTGGTTGTAATACGAAATTATCTAGTTGCGGTTCAGAGTCGCCACTTGGTAATGTCTCTTCCTTGGTAAATGAGAATGTGCATGAAAACCAAAGAACAGAGCAAGAGATCAAAACTTTCCTCAGCTTTCTTCATTCTGAAGCTTCAGAAGGTGCAAAAAAGGCTCAAGGGGTTCATCAAAAATTTGCAAAAATAGGTACCCTGATAGGACATGAAGCAGTTAAGGCAGAGGAGAGTAGTAGACTATCCTCTCCTAGTCCTAGGTCTTCACCGAGGCAACGACATAGCCCGAAACAAAAAGCAATGGATGCAGTTTCATCTATGGAAACTGCAGATCTCCACTCTGCTCCTACTACATTTATTGGGTCAAGTGATGGAGATTATCCGGTGATTAAAACCTTTAATGATGCCAAGACCATATGCTTTGTGGAGTCTCAAAAGCTTTGGGCCATTGCTGGTCCTATTGCCTTTAACATTTTGTGCAACTATGGTGTTAACTCCTTCACAAACATCTTTGTTGGGCATATTGGAAATTTGGAGCTCTCTGCTGTTGCAATTTCTCTGTCTGTCATTGCAAATTTCTCTTTTGGCTTTCTGCTTGGGATGGCAAGTGCACTTGAAACACTATGTGGGCAGGCATTTGGTGCTGGGCAAGTAGAAATGCTAGGAGTTTACATGCAACGATCATGGATAATTCTGATAATCACATGCTTTGGTTTACTACCACTTTATGTGTTCGCGACGCCAATCCTAAAACTCCTTGGCCAAGAAGATGACATTGCAAACCTTGCCGGGGAAGTTCTCTATCCAAACCATTCCTCAAATGTTTTCACTGGCCATCAATTTCCCAACTCAAAAGTTCTTGCAAGCACAGAGCAGGGTTTCGGTTCTGGCATGGATTGGTTTTGCAACTCTAATTCTACACATAGGAATCCTCTATGTCTTTATCGAATTGTTCGGGTGGGGAACGAGTGGTGCTGCTGCAGCCTATAA

Protein Analysis

1185

Amino Acids

129.92

Weight (kDa)

5.95

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 111 - 201 2.5e-26 D-mannose binding lectin
S_locus_glycop PF00954 241 - 353 1.9e-21 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 535 - 811 2.2e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 536 - 809 1.6e-39 Protein kinase domain
MatE PF01554 1009 - 1146 3e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
Acc65I GGTACC 2 cut(s) 954, 2762
AccB1I GGYRCC 5 cut(s) 290, 479, 954, 2206, 2762
AccBSI CCGCTC 4 cut(s) 504, 604, 858, 1637
AccI GTMKAC 3 cut(s) 917, 1285, 2806
AccII CGCG 4 cut(s) 354, 542, 629, 3305
AclWI GGATC 1 cut(s) 2120
AcoI YGGCCR 3 cut(s) 409, 3328, 3400
AcsI RAATTY 8 cut(s) 1309, 1366, 1555, 1867, 2521, 2747, 3097, 3142
AcuI CTGAAG 4 cut(s) 363, 1568, 2694, 2724
AcvI CACGTG 1 cut(s) 573
AcyI GRCGYC 4 cut(s) 291, 480, 824, 3308
AdeI CACNNNGTG 1 cut(s) 3194
AfeI AGCGCT 1 cut(s) 2490
AflII CTTAAG 1 cut(s) 2345
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 8 cut(s) 165, 1366, 1372, 1396, 1555, 1561, 1873, 3185
AjiI CACGTC 1 cut(s) 137
AjnI CCWGG 2 cut(s) 1696, 2119
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
Alw21I GWGCWC 3 cut(s) 1328, 3108, 3181
Alw26I GTCTC 9 cut(s) 28, 178, 929, 1013, 1305, 1762, 2040, 2626, 3003
Alw44I GTGCAC 1 cut(s) 3177
AlwI GGATC 1 cut(s) 2120
Ama87I CYCGRG 3 cut(s) 643, 1616, 2327
Aor51HI AGCGCT 1 cut(s) 2490
ApaLI GTGCAC 1 cut(s) 3177
ApeKI GCWGC 7 cut(s) 517, 1543, 1701, 2066, 3543, 3546, 3549
ApoI RAATTY 8 cut(s) 1309, 1366, 1555, 1867, 2521, 2747, 3097, 3142
Asp718I GGTACC 2 cut(s) 954, 2762
AspA2I CCTAGG 2 cut(s) 1645, 2825
AspLEI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
AsuC2I CCSGG 9 cut(s) 532, 563, 582, 644, 645, 730, 880, 1597, 3358
AsuNHI GCTAGC 2 cut(s) 1439, 1894
AvaI CYCGRG 3 cut(s) 643, 1616, 2327
AvaII GGWCC 8 cut(s) 255, 325, 634, 726, 865, 1832, 1862, 3023
AvrII CCTAGG 2 cut(s) 1645, 2825
AxyI CCTNAGG 2 cut(s) 963, 1045
BaeGI GKGCMC 1 cut(s) 3181
BalI TGGCCA 2 cut(s) 3330, 3402
BanI GGYRCC 5 cut(s) 290, 479, 954, 2206, 2762
BanII GRGCYC 2 cut(s) 1328, 3108
BarI GAAGNNNNNNTAC 2 cut(s) 2608, 2640
BbrPI CACGTG 1 cut(s) 573
BbsI GAAGAC 2 cut(s) 153, 2823
Bbv12I GWGCWC 3 cut(s) 1328, 3108, 3181
BbvCI CCTCAGC 1 cut(s) 2687
BbvI GCAGC 6 cut(s) 504, 1530, 1713, 2053, 3530, 3533
BceAI ACGGC 7 cut(s) 424, 458, 612, 832, 837, 1923, 2019
BciT130I CCWGG 2 cut(s) 1698, 2121
BcnI CCSGG 9 cut(s) 532, 563, 582, 644, 645, 730, 880, 1597, 3358
BcoDI GTCTC 9 cut(s) 28, 178, 929, 1013, 1305, 1762, 2040, 2626, 3003
BfaI CTAG 8 cut(s) 695, 1440, 1646, 1895, 2589, 2820, 2826, 3230
BfmI CTRYAG 3 cut(s) 2217, 2895, 3547
BfoI RGCGCY 4 cut(s) 294, 483, 2108, 2492
BfrI CTTAAG 1 cut(s) 2345
BglI GCCNNNNNGGC 1 cut(s) 299
BglII AGATCT 1 cut(s) 2899
BlnI CCTAGG 2 cut(s) 1645, 2825
BmcAI AGTACT 1 cut(s) 719
Bme18I GGWCC 8 cut(s) 255, 325, 634, 726, 865, 1832, 1862, 3023
BmeT110I CYCGRG 3 cut(s) 643, 1616, 2327
BmgBI CACGTC 1 cut(s) 137
BmrI ACTGGG 2 cut(s) 109, 1885
BmsI GCATC 3 cut(s) 1339, 2863, 2962
BmtI GCTAGC 2 cut(s) 1443, 1898
BmuI ACTGGG 2 cut(s) 109, 1885
BpiI GAAGAC 2 cut(s) 153, 2823
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 2 cut(s) 742, 2451
Bpu10I CCTNAGC 2 cut(s) 1410, 2687
BpuEI CTTGAG 2 cut(s) 220, 2715
BpuMI CCSGG 9 cut(s) 532, 563, 582, 644, 645, 730, 880, 1597, 3358
Bsa29I ATCGAT 1 cut(s) 2546
BsaAI YACGTR 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 2076
BsaHI GRCGYC 4 cut(s) 291, 480, 824, 3308
BsaI GGTCTC 1 cut(s) 1305
BsaWI WCCGGW 1 cut(s) 2950
BsaXI ACNNNNNCTCC 6 cut(s) 623, 653, 845, 875, 2449, 2479
Bse118I RCCGGY 2 cut(s) 411, 1546
Bse1I ACTGG 4 cut(s) 115, 725, 1880, 3403
Bse21I CCTNAGG 2 cut(s) 963, 1045
Bse3DI GCAATG 6 cut(s) 1917, 2305, 2874, 3015, 3135, 3342
Bse8I GATNNNNATC 1 cut(s) 2076
BseBI CCWGG 2 cut(s) 1698, 2121
BseCI ATCGAT 1 cut(s) 2546
BseGI GGATG 7 cut(s) 1069, 1354, 1512, 1885, 2427, 2878, 3174
BseJI GATNNNNATC 1 cut(s) 2076
BseMI GCAATG 6 cut(s) 1917, 2305, 2874, 3015, 3135, 3342
BseMII CTCAG 4 cut(s) 954, 1059, 1800, 2701
BseNI ACTGG 4 cut(s) 115, 725, 1880, 3403
BseRI GAGGAG 4 cut(s) 23, 304, 1536, 2810
BseSI GKGCMC 1 cut(s) 3181
BseX3I CGGCCG 1 cut(s) 409
BseXI GCAGC 6 cut(s) 504, 1530, 1713, 2053, 3530, 3533
BseYI CCCAGC 1 cut(s) 3212
BsgI GTGCAG 2 cut(s) 994, 2134
Bsh1236I CGCG 4 cut(s) 354, 542, 629, 3305
Bsh1285I CGRYCG 2 cut(s) 358, 412
BshNI GGYRCC 5 cut(s) 290, 479, 954, 2206, 2762
BshVI ATCGAT 1 cut(s) 2546
BsiEI CGRYCG 2 cut(s) 358, 412
BsiHKAI GWGCWC 3 cut(s) 1328, 3108, 3181
BsiHKCI CYCGRG 3 cut(s) 643, 1616, 2327
BslFI GGGAC 9 cut(s) 123, 311, 625, 752, 956, 1026, 1091, 1100, 1871
BsmAI GTCTC 9 cut(s) 28, 178, 929, 1013, 1305, 1762, 2040, 2626, 3003
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 9 cut(s) 123, 311, 625, 752, 956, 1026, 1091, 1100, 1871
Bso31I GGTCTC 1 cut(s) 1305
BsoBI CYCGRG 3 cut(s) 643, 1616, 2327
Bsp1286I GDGCHC 3 cut(s) 1328, 3108, 3181
Bsp143I GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
Bsp19I CCATGG 1 cut(s) 12
Bsp68I TCGCGA 1 cut(s) 3305
BspCNI CTCAG 4 cut(s) 955, 1058, 1799, 2700
BspDI ATCGAT 1 cut(s) 2546
BspFNI CGCG 4 cut(s) 354, 542, 629, 3305
BspMAI CTGCAG 2 cut(s) 2899, 3551
BspOI GCTAGC 2 cut(s) 1443, 1898
BspPI GGATC 1 cut(s) 2120
BspT107I GGYRCC 5 cut(s) 290, 479, 954, 2206, 2762
BspTI CTTAAG 1 cut(s) 2345
BspTNI GGTCTC 1 cut(s) 1305
BsrBI CCGCTC 4 cut(s) 504, 604, 858, 1637
BsrDI GCAATG 6 cut(s) 1917, 2305, 2874, 3015, 3135, 3342
BsrFI RCCGGY 2 cut(s) 411, 1546
BsrI ACTGG 4 cut(s) 115, 725, 1880, 3403
BssAI RCCGGY 2 cut(s) 411, 1546
BssMI GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
BssNAI GTATAC 1 cut(s) 918
BssNI GRCGYC 4 cut(s) 291, 480, 824, 3308
BssT1I CCWWGG 5 cut(s) 12, 1645, 2628, 2825, 3325
Bst1107I GTATAC 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 1698, 2121
Bst6I CTCTTC 3 cut(s) 33, 1505, 2629
BstACI GRCGYC 4 cut(s) 291, 480, 824, 3308
BstAFI CTTAAG 1 cut(s) 2345
BstAPI GCANNNNNTGC 4 cut(s) 2066, 2873, 3209, 3353
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 9 cut(s) 413, 454, 620, 1232, 1441, 1896, 2554, 3201, 3433
BstDEI CTNAG 7 cut(s) 963, 1045, 1410, 1772, 1786, 1838, 2687
BstDSI CCRYGG 2 cut(s) 12, 671
BstENI CCTNNNNNAGG 1 cut(s) 2348
BstF5I GGATG 7 cut(s) 1069, 1354, 1512, 1885, 2427, 2878, 3174
BstFNI CGCG 4 cut(s) 354, 542, 629, 3305
BstH2I RGCGCY 4 cut(s) 294, 483, 2108, 2492
BstHHI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
BstKTI GATC 6 cut(s) 559, 1392, 2128, 2676, 2902, 3251
BstMAI GTCTC 9 cut(s) 28, 178, 929, 1013, 1305, 1762, 2040, 2626, 3003
BstMBI GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
BstMCI CGRYCG 2 cut(s) 358, 412
BstNI CCWGG 2 cut(s) 1698, 2121
BstNSI RCATGY 4 cut(s) 1757, 1825, 3244, 3276
BstSFI CTRYAG 3 cut(s) 2217, 2895, 3547
BstSLI GKGCMC 1 cut(s) 3181
BstUI CGCG 4 cut(s) 354, 542, 629, 3305
BstV1I GCAGC 6 cut(s) 504, 1530, 1713, 2053, 3530, 3533
BstV2I GAAGAC 2 cut(s) 153, 2823
BstX2I RGATCY 1 cut(s) 2899
BstYI RGATCY 1 cut(s) 2899
BstZ17I GTATAC 1 cut(s) 918
BstZI CGGCCG 1 cut(s) 409
Bsu15I ATCGAT 1 cut(s) 2546
Bsu36I CCTNAGG 2 cut(s) 963, 1045
BsuTUI ATCGAT 1 cut(s) 2546
BtgI CCRYGG 2 cut(s) 12, 671
BtrI CACGTC 1 cut(s) 137
BtsCI GGATG 7 cut(s) 1069, 1354, 1512, 1885, 2427, 2878, 3174
BtsI GCAGTG 1 cut(s) 1683
BtsIMutI CAGTG 2 cut(s) 1683, 3396
BtuMI TCGCGA 1 cut(s) 3305
Cac8I GCNNGC 9 cut(s) 413, 454, 620, 1232, 1441, 1896, 2554, 3201, 3433
CfoI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
Cfr10I RCCGGY 2 cut(s) 411, 1546
Cfr9I CCCGGG 1 cut(s) 643
ClaI ATCGAT 1 cut(s) 2546
CseI GACGC 2 cut(s) 813, 3316
CsiI ACCWGGT 1 cut(s) 2119
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
DdeI CTNAG 7 cut(s) 963, 1045, 1410, 1772, 1786, 1838, 2687
DinI GGCGCC 2 cut(s) 292, 481
DpnI GATC 6 cut(s) 558, 1391, 2127, 2675, 2901, 3250
DpnII GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
DraI TTTAAA 1 cut(s) 1606
DraIII CACNNNGTG 1 cut(s) 3194
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 3 cut(s) 409, 3328, 3400
EagI CGGCCG 1 cut(s) 409
Eam1104I CTCTTC 3 cut(s) 33, 1505, 2629
EarI CTCTTC 3 cut(s) 33, 1505, 2629
EciI GGCGGA 2 cut(s) 614, 637
Ecl136II GAGCTC 2 cut(s) 1326, 3106
EclXI CGGCCG 1 cut(s) 409
Eco130I CCWWGG 5 cut(s) 12, 1645, 2628, 2825, 3325
Eco147I AGGCCT 1 cut(s) 2410
Eco24I GRGCYC 2 cut(s) 1328, 3108
Eco31I GGTCTC 1 cut(s) 1305
Eco32I GATATC 1 cut(s) 1966
Eco47I GGWCC 8 cut(s) 255, 325, 634, 726, 865, 1832, 1862, 3023
Eco47III AGCGCT 1 cut(s) 2490
Eco52I CGGCCG 1 cut(s) 409
Eco53kI GAGCTC 2 cut(s) 1326, 3106
Eco57I CTGAAG 4 cut(s) 363, 1568, 2694, 2724
Eco72I CACGTG 1 cut(s) 573
Eco81I CCTNAGG 2 cut(s) 963, 1045
Eco88I CYCGRG 3 cut(s) 643, 1616, 2327
EcoICRI GAGCTC 2 cut(s) 1326, 3106
EcoNI CCTNNNNNAGG 1 cut(s) 2348
EcoO109I RGGNCCY 4 cut(s) 255, 325, 607, 634
EcoRI GAATTC 1 cut(s) 2521
EcoRII CCWGG 2 cut(s) 1696, 2119
EcoRV GATATC 1 cut(s) 1966
EcoT14I CCWWGG 5 cut(s) 12, 1645, 2628, 2825, 3325
EcoT38I GRGCYC 2 cut(s) 1328, 3108
EgeI GGCGCC 2 cut(s) 292, 481
EheI GGCGCC 2 cut(s) 292, 481
ErhI CCWWGG 5 cut(s) 12, 1645, 2628, 2825, 3325
Esp3I CGTCTC 1 cut(s) 28
FalI AAGNNNNNCTT 2 cut(s) 1214, 1246
FaqI GGGAC 9 cut(s) 123, 311, 625, 752, 956, 1026, 1091, 1100, 1871
FauI CCCGC 3 cut(s) 597, 851, 876
FauNDI CATATG 1 cut(s) 2984
FblI GTMKAC 3 cut(s) 917, 1285, 2806
FokI GGATG 7 cut(s) 1076, 1361, 1519, 1892, 2434, 2885, 3181
FriOI GRGCYC 2 cut(s) 1328, 3108
FspBI CTAG 8 cut(s) 695, 1440, 1646, 1895, 2589, 2820, 2826, 3230
GlaI GCGC 6 cut(s) 292, 481, 628, 668, 2106, 2490
GsaI CCCAGC 1 cut(s) 3216
GsuI CTGGAG 2 cut(s) 742, 2451
HaeII RGCGCY 4 cut(s) 294, 483, 2108, 2492
HgaI GACGC 2 cut(s) 813, 3316
HhaI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
Hin1I GRCGYC 4 cut(s) 291, 480, 824, 3308
Hin6I GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
HinP1I GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
HincII GTYRAC 1 cut(s) 3061
HindII GTYRAC 1 cut(s) 3061
HindIII AAGCTT 2 cut(s) 2706, 3005
HpaI GTTAAC 1 cut(s) 3061
Hpy188III TCNNGA 9 cut(s) 165, 175, 367, 886, 1393, 1856, 2329, 2532, 3304
Hpy99I CGWCG 7 cut(s) 410, 479, 823, 829, 1857, 2399, 3310
HpyCH4IV ACGT 4 cut(s) 136, 572, 791, 1852
HpyF3I CTNAG 7 cut(s) 963, 1045, 1410, 1772, 1786, 1838, 2687
HpySE526I ACGT 4 cut(s) 136, 572, 791, 1852
Hsp92I GRCGYC 4 cut(s) 291, 480, 824, 3308
HspAI GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
KasI GGCGCC 2 cut(s) 290, 479
KpnI GGTACC 2 cut(s) 958, 2766
KroI GCCGGC 1 cut(s) 411
KroNI GCCGGC 1 cut(s) 413
KspAI GTTAAC 1 cut(s) 3061
Kzo9I GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
Lsp1109I GCAGC 6 cut(s) 504, 1530, 1713, 2053, 3530, 3533
LweI GCATC 3 cut(s) 1339, 2863, 2962
MabI ACCWGGT 1 cut(s) 2119
MaeI CTAG 8 cut(s) 695, 1440, 1646, 1895, 2589, 2820, 2826, 3230
MaeII ACGT 4 cut(s) 136, 572, 791, 1852
MaeIII GTNAC 5 cut(s) 743, 1456, 2428, 2455, 2481
MalI GATC 6 cut(s) 558, 1391, 2127, 2675, 2901, 3250
MbiI CCGCTC 4 cut(s) 504, 604, 858, 1637
MboI GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
MflI RGATCY 1 cut(s) 2899
MhlI GDGCHC 3 cut(s) 1328, 3108, 3181
MlsI TGGCCA 2 cut(s) 3330, 3402
MluNI TGGCCA 2 cut(s) 3330, 3402
Mly113I GGCGCC 2 cut(s) 291, 480
MlyI GAGTC 9 cut(s) 381, 422, 436, 1073, 1076, 2319, 2513, 2612, 3005
MmeI TCCRAC 5 cut(s) 147, 477, 831, 843, 2418
Mox20I TGGCCA 2 cut(s) 3330, 3402
MroNI GCCGGC 1 cut(s) 411
MscI TGGCCA 2 cut(s) 3330, 3402
Msp20I TGGCCA 2 cut(s) 3330, 3402
MspA1I CMGCKG 3 cut(s) 618, 874, 1704
MspCI CTTAAG 1 cut(s) 2345
MvaI CCWGG 2 cut(s) 1698, 2121
MvnI CGCG 4 cut(s) 354, 542, 629, 3305
NaeI GCCGGC 1 cut(s) 413
NarI GGCGCC 2 cut(s) 291, 480
NciI CCSGG 9 cut(s) 532, 563, 582, 644, 645, 730, 880, 1597, 3358
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 2984
NdeII GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
NgoMIV GCCGGC 1 cut(s) 411
NheI GCTAGC 2 cut(s) 1439, 1894
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 743, 2428, 2455
NruI TCGCGA 1 cut(s) 3305
NspI RCATGY 4 cut(s) 1757, 1825, 3244, 3276
PceI AGGCCT 1 cut(s) 2410
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 414
PdiI GCCGGC 1 cut(s) 413
PfeI GAWTC 8 cut(s) 181, 370, 589, 1793, 1946, 2252, 2528, 3496
PflFI GACNNNGTC 2 cut(s) 823, 2538
PleI GAGTC 9 cut(s) 381, 422, 436, 1073, 1075, 2319, 2512, 2611, 3004
PluTI GGCGCC 2 cut(s) 294, 483
PmaCI CACGTG 1 cut(s) 573
PmlI CACGTG 1 cut(s) 573
PpsI GAGTC 9 cut(s) 381, 422, 436, 1073, 1075, 2319, 2512, 2611, 3004
Ppu21I YACGTR 1 cut(s) 573
PpuMI RGGWCCY 3 cut(s) 255, 325, 634
Psp124BI GAGCTC 2 cut(s) 1328, 3108
Psp5II RGGWCCY 3 cut(s) 255, 325, 634
Psp6I CCWGG 2 cut(s) 1696, 2119
PspCI CACGTG 1 cut(s) 573
PspFI CCCAGC 1 cut(s) 3212
PspGI CCWGG 2 cut(s) 1696, 2119
PspPPI RGGWCCY 3 cut(s) 255, 325, 634
PstI CTGCAG 2 cut(s) 2899, 3551
PsuI RGATCY 1 cut(s) 2899
PsyI GACNNNGTC 2 cut(s) 823, 2538
PvuII CAGCTG 1 cut(s) 618
RruI TCGCGA 1 cut(s) 3305
SacI GAGCTC 2 cut(s) 1328, 3108
Sau3AI GATC 6 cut(s) 556, 1389, 2125, 2673, 2899, 3248
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 9 cut(s) 381, 422, 436, 1073, 1076, 2319, 2513, 2612, 3005
SduI GDGCHC 3 cut(s) 1328, 3108, 3181
SexAI ACCWGGT 1 cut(s) 2119
SfaNI GCATC 3 cut(s) 1339, 2863, 2962
SfcI CTRYAG 3 cut(s) 2217, 2895, 3547
SfoI GGCGCC 2 cut(s) 292, 481
SinI GGWCC 8 cut(s) 255, 325, 634, 726, 865, 1832, 1862, 3023
SmaI CCCGGG 1 cut(s) 645
SmlI CTYRAG 3 cut(s) 199, 2345, 2730
SmoI CTYRAG 3 cut(s) 199, 2345, 2730
SseBI AGGCCT 1 cut(s) 2410
SspDI GGCGCC 2 cut(s) 290, 479
SspMI CTAG 8 cut(s) 695, 1440, 1646, 1895, 2589, 2820, 2826, 3230
SstI GAGCTC 2 cut(s) 1328, 3108
StuI AGGCCT 1 cut(s) 2410
StyI CCWWGG 5 cut(s) 12, 1645, 2628, 2825, 3325
TaiI ACGT 4 cut(s) 139, 575, 794, 1855
TaqI TCGA 8 cut(s) 176, 390, 440, 555, 1196, 1307, 2546, 3514
TatI WGTACW 3 cut(s) 309, 717, 1260
TauI GCSGC 7 cut(s) 291, 467, 470, 473, 507, 874, 2279
TfiI GAWTC 8 cut(s) 181, 370, 589, 1793, 1946, 2252, 2528, 3496
TscAI CASTG 2 cut(s) 1683, 3403
TseFI GTSAC 3 cut(s) 743, 2428, 2455
TseI GCWGC 7 cut(s) 517, 1543, 1701, 2066, 3543, 3546, 3549
Tsp45I GTSAC 3 cut(s) 743, 2428, 2455
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 2 cut(s) 1683, 3403
Tth111I GACNNNGTC 2 cut(s) 823, 2538
Vha464I CTTAAG 1 cut(s) 2345
VneI GTGCAC 1 cut(s) 3177
VpaK11BI GGWCC 8 cut(s) 255, 325, 634, 726, 865, 1832, 1862, 3023
XagI CCTNNNNNAGG 1 cut(s) 2348
XapI RAATTY 8 cut(s) 1309, 1366, 1555, 1867, 2521, 2747, 3097, 3142
XceI RCATGY 4 cut(s) 1757, 1825, 3244, 3276
XcmI CCANNNNNNNNNTGG 2 cut(s) 1872, 2989
XmaI CCCGGG 1 cut(s) 643
XmaJI CCTAGG 2 cut(s) 1645, 2825
XmiI GTMKAC 3 cut(s) 917, 1285, 2806
XspI CTAG 8 cut(s) 695, 1440, 1646, 1895, 2589, 2820, 2826, 3230
ZrmI AGTACT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.