Rw1G023430

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0011114
Physical Location & Seq
Reverse (-)
49202205 .. 49205146
2942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023430.1

Sequence Viewer

Length: 2694 bp
ATGGCTTCTTCCATGGTTCCCATCTCTCTCCTCCTCCCCATACTCCAAACCCTGATTCTCATCTCCACAAACTCCAATCCCATTCCCAAAACTACCTCACCCCAAAGCCCAACCCAGTTCTCCAACACCCTTCCATATCCCACTCCAAACTCAAACGGGTCTTCAAGAACTGTCTCGAAGATTCTCTGGTACGCACCCTCGAGAACCAACAAGGTTATAGTCCATGGCAACTCCACCATTTGCAGTCGCAACAGGACCTTTCAGCTCGGCTTCTTCCCCTTTAATGGCGGCGCCTACTTGGCAATAATGTACTCCTCAATTCAGGTCCCCACCGTAGTCTGGGTCGCCAACCGCGACCGCCCCGTCAAGAATCTCACTTCAGCCAGACTCGAAATCACAGCCACCGACGGCCGGCTCGTCGTTATAGACTCCGATAACTCGACTCTCTGGGCCAGCCAGAACGCCGCCGTCGGCGCCCAAGTCGTGCTCTTGGAGAGCGGCAATCTGGTGCTGCTCACCCGCTCCGGGGAACTCGCGTGGCAGAGCTTCGATCACCCGGCTGACACGTGGCTCCCCGGAATGAATCTGACGGCGGAGCGGGGCCTGACCAGCTGGCGGAGCGCGGAGGACCCGTCCCCGGGTTTGTATTCGCTGCGGTTGCGCCCACAGGATTACGGTGAGTTTGAGCTCGTTTTTAACGGTAGTGTTGAGTACTGGAGGACCGGGGTCTGGGACGGTCACGCTTTTGCTAATGTGCCGGAGATGATTGTTAAGTATATCTATACGTTTCACTTCATAGACCCGTACAAGCCGACGGCGTCGTTTTGGTTCACCGAGGGGCCGTTGGAGAGCGGGTTGGACCCGCCGCTGACCCGGTTCCAGATAGACCATTCGGGTCAGCTCCGGCAGTATACTTGGGGTTTAAGCGAGACCTGGGACAAGTTTTGGTACCGGCCTGAGGACAAGTGCAGAGTGTTTGGTTTGTGTGGGAGGTTTGGGGTTTGTGATAGTGAGACCGCTGAACCCTGTGAGTGTCCCTCAGGGTTTAAGCCTGTGGATGAGTCTGGTTGGGACTCTGGGGACTATTCCGGTGGGTGTGAGAGGTTTGGTGATGTGCCTTGTGAGGAGAGAGATGAGGGTTTCGTGGAGGTTCAGGTTGAGAGTTTTGCGGAAAGTTGGCATAATAGTTCGATTACTGGTAGGGATGGTTGTGAAAAGATGTGCTTGCAAGTTTGTTCTTGTATTGGTTTTCAGTACAACGAGAGGTCTAGTTTGTGTAGACATTATTTCGGGTCTGTGTTGAATTTGAGGAATGTGAGCTCTGATGGTAATAGCAGTATGGATGCTGGTAGTGTTTTGAATTTGAAGGTACGAAAAGGTGTGATCTTGAAGAACAGGAAGGCTAAGGTTATGAATTATACAGTTTTTGGTGCTAGCATTGTTGGGTCAGTTGCGGTTTTGGGTTTTCTGGTGGTGGTTGTATTGATTAGGAGAAGAAGGATGAAGAGGAATGGAGGAGCAGAGGAAAGTGTTTTGCTGCCGGTGTTGAATTTGAAGGTATTTTCCTACAAAGAGCTTCAGACTGCAACCTGGGGTTTTAAAGAGAAACTCGGGCATGGTGGCTTTGGAGCGGTTTTCCTAGGGGAGCTATCAGACCGTACTCTTGTTGCAGTGAAACGCTTGGAGAGGCCAGGCAGCGGAGAGCAACAATTCCGAGCAGAAGTTTGCACCATTGGCAACATTCAGCATGTCAATCTTGTGAGACTCAGAGGCTTCTGCTCAGAAGATTCTCATAGACTTCTGGTATATGATTACATGCCAAGTGGTCCTCTAAGTGTGTATCTACGTCGAGACGGTCCAAATTTGAGCTGGGATGTTAGGTTTCGGCTAGCAGTTGGTACGGCAAGAGGCATTGCATATTTACATGAGGGGTGTAGAGATTCCATAATCCATTGTGATATCAAACCAGAAAACATTTTGCTTGATTGTGATTACACGGCAAAGGTGTCAGATTTTGGGTTAGCAAAGCTAATTCACAGAGACTTTAGTGGAGCATTAGCTGCTGATGGTAATCATGGCTATACCTATGGCTATGTAGCGCCAGAGTGCAGACCTGGTGATCCAATCACCACAAAAGCCGATGTTTACAGCTATGGAAAGACATTGATAGAATTACTTGGAGGCCATAGAATGTGGGAGGCACCTCCATCTGCAGGCGGCAGAGAGGGTGGAAATGAAATAACAGATTCTTGGTGCTTTCCTTCGTGGGCGGCACAGCAGATAATTGATGGCAATGTGGTGGCTGTAATTGATGATAGACTCGGGAGCAAGTATAACCTTAAGGAGGCAGAGCGTGTTGCATTAGTGGCGGTGTGGTGCATACAGGACGACGAGGCAACAAGGCCTACTATGGGGATGGTAGTGACAATGTTGGAAGGGGTTGTGGAAGTGACTGTTCCTCCAGCACCAAAGTTGTTACAAGCGCTGGTTTCAGGGGAGTCATTCTGTGGGGTGAATTCCGATTCTGGACACAGTCCATCGATTGCCAGCGACAACTTCTCTGGTTGTAATACGAAATTATCTAGTTGCGGTTCAGAGTCGTCACTTGGTAATGTCTCTTCCTTAGTAAATGAGAATGTGCATGAAAACCGAAGAAGTTCAGTGAGCTTTACTGTCAATACTGACCATAGCTAA

Protein Analysis

897

Amino Acids

98.83

Weight (kDa)

5.85

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 111 - 199 1.5e-25 D-mannose binding lectin
S_locus_glycop PF00954 239 - 351 2.2e-21 S-locus glycoprotein domain
Pkinase PF00069 533 - 807 3.2e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 533 - 809 5.7e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
Acc65I GGTACC 1 cut(s) 948
AccB1I GGYRCC 4 cut(s) 290, 473, 948, 2200
AccBSI CCGCTC 5 cut(s) 498, 522, 598, 852, 1631
AccI GTMKAC 2 cut(s) 911, 1279
AccII CGCG 3 cut(s) 354, 536, 623
AclWI GGATC 1 cut(s) 2114
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 5 cut(s) 1303, 1360, 1549, 1861, 2515
AcuI CTGAAG 2 cut(s) 363, 1562
AcvI CACGTG 1 cut(s) 567
AcyI GRCGYC 3 cut(s) 291, 474, 818
AfaI GTAC 9 cut(s) 191, 311, 713, 806, 950, 1256, 1371, 1660, 1900
AfeI AGCGCT 1 cut(s) 2484
AflII CTTAAG 1 cut(s) 2339
AflIII ACRYGT 1 cut(s) 564
AgsI TTSAA 7 cut(s) 165, 1303, 1360, 1366, 1390, 1549, 1555
AjnI CCWGG 4 cut(s) 932, 1589, 1690, 2113
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
Alw21I GWGCWC 3 cut(s) 489, 690, 1322
Alw26I GTCTC 7 cut(s) 178, 923, 1007, 1756, 1845, 2034, 2620
AlwI GGATC 1 cut(s) 2114
Ama87I CYCGRG 4 cut(s) 199, 637, 1610, 2321
Aor51HI AGCGCT 1 cut(s) 2484
AoxI GGCC 8 cut(s) 409, 450, 601, 839, 953, 1688, 2182, 2402
ApeKI GCWGC 5 cut(s) 511, 652, 1537, 1695, 2060
ApoI RAATTY 5 cut(s) 1303, 1360, 1549, 1861, 2515
Asp700I GAANNNNTTC 1 cut(s) 2656
Asp718I GGTACC 1 cut(s) 948
AspA2I CCTAGG 1 cut(s) 1639
AspLEI GCGC 6 cut(s) 293, 476, 623, 663, 2101, 2485
AsuC2I CCSGG 7 cut(s) 526, 557, 576, 638, 639, 724, 874
AsuHPI GGTGA 9 cut(s) 90, 508, 545, 689, 823, 1121, 2119, 2129, 2524
AsuNHI GCTAGC 2 cut(s) 1433, 1888
AvaI CYCGRG 4 cut(s) 199, 637, 1610, 2321
AvaII GGWCC 7 cut(s) 255, 325, 628, 720, 859, 1826, 1856
AvrII CCTAGG 1 cut(s) 1639
AxyI CCTNAGG 2 cut(s) 957, 1039
BanI GGYRCC 4 cut(s) 290, 473, 948, 2200
BanII GRGCYC 2 cut(s) 690, 1322
BarI GAAGNNNNNNTAC 2 cut(s) 2602, 2634
BbrPI CACGTG 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 3 cut(s) 489, 690, 1322
BbvI GCAGC 5 cut(s) 498, 639, 1524, 1707, 2047
BccI CCATC 8 cut(s) 29, 1199, 1319, 2060, 2215, 2282, 2410, 2545
BceAI ACGGC 7 cut(s) 424, 452, 606, 826, 831, 1917, 2013
BciT130I CCWGG 4 cut(s) 934, 1591, 1692, 2115
BcnI CCSGG 7 cut(s) 526, 557, 576, 638, 639, 724, 874
BcoDI GTCTC 7 cut(s) 178, 923, 1007, 1756, 1845, 2034, 2620
BfaI CTAG 5 cut(s) 1269, 1434, 1640, 1889, 2583
BfmI CTRYAG 1 cut(s) 2211
BfoI RGCGCY 4 cut(s) 294, 477, 2102, 2486
BfrI CTTAAG 1 cut(s) 2339
BglI GCCNNNNNGGC 1 cut(s) 299
BlnI CCTAGG 1 cut(s) 1639
BmcAI AGTACT 1 cut(s) 713
Bme18I GGWCC 7 cut(s) 255, 325, 628, 720, 859, 1826, 1856
BmeT110I CYCGRG 4 cut(s) 199, 637, 1610, 2321
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 1333
BmtI GCTAGC 2 cut(s) 1437, 1892
BmuI ACTGGG 1 cut(s) 109
BoxI GACNNNNGTC 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpmI CTGGAG 2 cut(s) 736, 2445
Bpu10I CCTNAGC 1 cut(s) 1404
BpuMI CCSGG 7 cut(s) 526, 557, 576, 638, 639, 724, 874
Bsa29I ATCGAT 1 cut(s) 2540
BsaAI YACGTR 1 cut(s) 567
BsaBI GATNNNNATC 2 cut(s) 59, 2070
BsaHI GRCGYC 3 cut(s) 291, 474, 818
BsaI GGTCTC 2 cut(s) 923, 1007
BsaWI WCCGGW 1 cut(s) 1088
BsaXI ACNNNNNCTCC 6 cut(s) 617, 647, 839, 869, 2443, 2473
Bse118I RCCGGY 3 cut(s) 411, 951, 1540
Bse1I ACTGG 3 cut(s) 115, 719, 1201
Bse21I CCTNAGG 2 cut(s) 957, 1039
Bse3DI GCAATG 2 cut(s) 1911, 2299
Bse8I GATNNNNATC 2 cut(s) 59, 2070
BseBI CCWGG 4 cut(s) 934, 1591, 1692, 2115
BseCI ATCGAT 1 cut(s) 2540
BseGI GGATG 6 cut(s) 1063, 1210, 1348, 1506, 1879, 2421
BseJI GATNNNNATC 2 cut(s) 59, 2070
BseMI GCAATG 2 cut(s) 1911, 2299
BseMII CTCAG 4 cut(s) 948, 1053, 1780, 1794
BseNI ACTGG 3 cut(s) 115, 719, 1201
BseRI GAGGAG 5 cut(s) 20, 23, 304, 1139, 1530
BseX3I CGGCCG 1 cut(s) 409
BseXI GCAGC 5 cut(s) 498, 639, 1524, 1707, 2047
BseYI CCCAGC 1 cut(s) 1869
BsgI GTGCAG 2 cut(s) 988, 2128
Bsh1236I CGCG 3 cut(s) 354, 536, 623
Bsh1285I CGRYCG 2 cut(s) 358, 412
BshFI GGCC 8 cut(s) 411, 452, 603, 841, 955, 1690, 2184, 2404
BshNI GGYRCC 4 cut(s) 290, 473, 948, 2200
BshVI ATCGAT 1 cut(s) 2540
BsiEI CGRYCG 2 cut(s) 358, 412
BsiHKAI GWGCWC 3 cut(s) 489, 690, 1322
BsiHKCI CYCGRG 4 cut(s) 199, 637, 1610, 2321
BslFI GGGAC 7 cut(s) 311, 619, 746, 950, 1020, 1085, 1094
BsmAI GTCTC 7 cut(s) 178, 923, 1007, 1756, 1845, 2034, 2620
BsmBI CGTCTC 1 cut(s) 1845
BsmFI GGGAC 7 cut(s) 311, 619, 746, 950, 1020, 1085, 1094
BsnI GGCC 8 cut(s) 411, 452, 603, 841, 955, 1690, 2184, 2404
Bso31I GGTCTC 2 cut(s) 923, 1007
BsoBI CYCGRG 4 cut(s) 199, 637, 1610, 2321
Bsp1286I GDGCHC 3 cut(s) 489, 690, 1322
Bsp143I GATC 3 cut(s) 550, 1383, 2119
Bsp19I CCATGG 2 cut(s) 12, 223
BspANI GGCC 8 cut(s) 411, 452, 603, 841, 955, 1690, 2184, 2404
BspCNI CTCAG 4 cut(s) 949, 1052, 1779, 1793
BspDI ATCGAT 1 cut(s) 2540
BspFNI CGCG 3 cut(s) 354, 536, 623
BspMAI CTGCAG 1 cut(s) 2215
BspOI GCTAGC 2 cut(s) 1437, 1892
BspPI GGATC 1 cut(s) 2114
BspT107I GGYRCC 4 cut(s) 290, 473, 948, 2200
BspTI CTTAAG 1 cut(s) 2339
BspTNI GGTCTC 2 cut(s) 923, 1007
BsrBI CCGCTC 5 cut(s) 498, 522, 598, 852, 1631
BsrDI GCAATG 2 cut(s) 1911, 2299
BsrFI RCCGGY 3 cut(s) 411, 951, 1540
BsrI ACTGG 3 cut(s) 115, 719, 1201
BssAI RCCGGY 3 cut(s) 411, 951, 1540
BssMI GATC 3 cut(s) 550, 1383, 2119
BssNAI GTATAC 1 cut(s) 912
BssNI GRCGYC 3 cut(s) 291, 474, 818
BssT1I CCWWGG 3 cut(s) 12, 223, 1639
Bst1107I GTATAC 1 cut(s) 912
Bst2UI CCWGG 4 cut(s) 934, 1591, 1692, 2115
Bst6I CTCTTC 2 cut(s) 1499, 2623
BstACI GRCGYC 3 cut(s) 291, 474, 818
BstAFI CTTAAG 1 cut(s) 2339
BstAPI GCANNNNNTGC 1 cut(s) 2060
BstBAI YACGTR 1 cut(s) 567
BstC8I GCNNGC 8 cut(s) 413, 454, 614, 1226, 1435, 1890, 2215, 2548
BstDEI CTNAG 7 cut(s) 957, 1039, 1404, 1766, 1780, 1832, 2623
BstDSI CCRYGG 2 cut(s) 12, 223
BstENI CCTNNNNNAGG 1 cut(s) 2342
BstF5I GGATG 6 cut(s) 1063, 1210, 1348, 1506, 1879, 2421
BstFNI CGCG 3 cut(s) 354, 536, 623
BstH2I RGCGCY 4 cut(s) 294, 477, 2102, 2486
BstHHI GCGC 6 cut(s) 293, 476, 623, 663, 2101, 2485
BstKTI GATC 3 cut(s) 553, 1386, 2122
BstMAI GTCTC 7 cut(s) 178, 923, 1007, 1756, 1845, 2034, 2620
BstMBI GATC 3 cut(s) 550, 1383, 2119
BstMCI CGRYCG 2 cut(s) 358, 412
BstMWI GCNNNNNNNGC 8 cut(s) 299, 473, 609, 618, 658, 1734, 2060, 2366
BstNI CCWGG 4 cut(s) 934, 1591, 1692, 2115
BstNSI RCATGY 2 cut(s) 1751, 1819
BstPAI GACNNNNGTC 1 cut(s) 725
BstSFI CTRYAG 1 cut(s) 2211
BstUI CGCG 3 cut(s) 354, 536, 623
BstV1I GCAGC 5 cut(s) 498, 639, 1524, 1707, 2047
BstV2I GAAGAC 1 cut(s) 153
BstZ17I GTATAC 1 cut(s) 912
BstZI CGGCCG 1 cut(s) 409
Bsu15I ATCGAT 1 cut(s) 2540
Bsu36I CCTNAGG 2 cut(s) 957, 1039
BsuRI GGCC 8 cut(s) 411, 452, 603, 841, 955, 1690, 2184, 2404
BsuTUI ATCGAT 1 cut(s) 2540
BtgI CCRYGG 2 cut(s) 12, 223
BtsCI GGATG 6 cut(s) 1063, 1210, 1348, 1506, 1879, 2421
BtsI GCAGTG 1 cut(s) 1677
BtsIMutI CAGTG 2 cut(s) 1677, 2667
Cac8I GCNNGC 8 cut(s) 413, 454, 614, 1226, 1435, 1890, 2215, 2548
CfoI GCGC 6 cut(s) 293, 476, 623, 663, 2101, 2485
Cfr10I RCCGGY 3 cut(s) 411, 951, 1540
Cfr9I CCCGGG 1 cut(s) 637
ClaI ATCGAT 1 cut(s) 2540
CseI GACGC 1 cut(s) 807
CsiI ACCWGGT 1 cut(s) 2113
Csp6I GTAC 9 cut(s) 190, 310, 712, 805, 949, 1255, 1370, 1659, 1899
CviAII CATG 8 cut(s) 13, 224, 1616, 1748, 1816, 1925, 2075, 2642
CviQI GTAC 9 cut(s) 190, 310, 712, 805, 949, 1255, 1370, 1659, 1899
DdeI CTNAG 7 cut(s) 957, 1039, 1404, 1766, 1780, 1832, 2623
DinI GGCGCC 2 cut(s) 292, 475
DpnI GATC 3 cut(s) 552, 1385, 2121
DpnII GATC 3 cut(s) 550, 1383, 2119
DraI TTTAAA 1 cut(s) 1600
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 409
EagI CGGCCG 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 1499, 2623
EarI CTCTTC 2 cut(s) 1499, 2623
EciI GGCGGA 2 cut(s) 608, 631
Ecl136II GAGCTC 2 cut(s) 688, 1320
EclXI CGGCCG 1 cut(s) 409
Eco130I CCWWGG 3 cut(s) 12, 223, 1639
Eco147I AGGCCT 1 cut(s) 2404
Eco24I GRGCYC 2 cut(s) 690, 1322
Eco31I GGTCTC 2 cut(s) 923, 1007
Eco32I GATATC 1 cut(s) 1960
Eco47I GGWCC 7 cut(s) 255, 325, 628, 720, 859, 1826, 1856
Eco47III AGCGCT 1 cut(s) 2484
Eco52I CGGCCG 1 cut(s) 409
Eco53kI GAGCTC 2 cut(s) 688, 1320
Eco57I CTGAAG 2 cut(s) 363, 1562
Eco72I CACGTG 1 cut(s) 567
Eco81I CCTNAGG 2 cut(s) 957, 1039
Eco88I CYCGRG 4 cut(s) 199, 637, 1610, 2321
EcoICRI GAGCTC 2 cut(s) 688, 1320
EcoNI CCTNNNNNAGG 1 cut(s) 2342
EcoO109I RGGNCCY 4 cut(s) 255, 325, 601, 628
EcoRI GAATTC 1 cut(s) 2515
EcoRII CCWGG 4 cut(s) 932, 1589, 1690, 2113
EcoRV GATATC 1 cut(s) 1960
EcoT14I CCWWGG 3 cut(s) 12, 223, 1639
EcoT38I GRGCYC 2 cut(s) 690, 1322
EgeI GGCGCC 2 cut(s) 292, 475
EheI GGCGCC 2 cut(s) 292, 475
ErhI CCWWGG 3 cut(s) 12, 223, 1639
Esp3I CGTCTC 1 cut(s) 1845
FaeI CATG 8 cut(s) 16, 227, 1619, 1751, 1819, 1928, 2078, 2645
FalI AAGNNNNNCTT 2 cut(s) 1208, 1240
FaqI GGGAC 7 cut(s) 311, 619, 746, 950, 1020, 1085, 1094
FatI CATG 8 cut(s) 12, 223, 1615, 1747, 1815, 1924, 2074, 2641
FauI CCCGC 4 cut(s) 527, 591, 845, 870
FblI GTMKAC 2 cut(s) 911, 1279
FokI GGATG 6 cut(s) 1070, 1217, 1355, 1513, 1886, 2428
FriOI GRGCYC 2 cut(s) 690, 1322
FspBI CTAG 5 cut(s) 1269, 1434, 1640, 1889, 2583
GlaI GCGC 6 cut(s) 292, 475, 622, 662, 2100, 2484
GsaI CCCAGC 1 cut(s) 1873
GsuI CTGGAG 2 cut(s) 736, 2445
HaeII RGCGCY 4 cut(s) 294, 477, 2102, 2486
HaeIII GGCC 8 cut(s) 411, 452, 603, 841, 955, 1690, 2184, 2404
HgaI GACGC 1 cut(s) 807
HhaI GCGC 6 cut(s) 293, 476, 623, 663, 2101, 2485
Hin1I GRCGYC 3 cut(s) 291, 474, 818
Hin1II CATG 8 cut(s) 16, 227, 1619, 1751, 1819, 1928, 2078, 2645
Hin6I GCGC 6 cut(s) 291, 474, 621, 661, 2099, 2483
HinP1I GCGC 6 cut(s) 291, 474, 621, 661, 2099, 2483
HphI GGTGA 9 cut(s) 90, 508, 545, 689, 823, 1121, 2119, 2129, 2524
Hpy166II GTNNAC 4 cut(s) 831, 912, 1280, 2146
Hpy188III TCNNGA 9 cut(s) 165, 175, 201, 367, 880, 1387, 1850, 2323, 2526
Hpy8I GTNNAC 4 cut(s) 831, 912, 1280, 2146
Hpy99I CGWCG 7 cut(s) 410, 422, 473, 817, 823, 1851, 2393
HpyAV CCTTC 7 cut(s) 140, 1360, 1393, 1491, 1549, 2271, 2429
HpyCH4IV ACGT 3 cut(s) 566, 785, 1846
HpyF10VI GCNNNNNNNGC 8 cut(s) 299, 473, 609, 618, 658, 1734, 2060, 2366
HpyF3I CTNAG 7 cut(s) 957, 1039, 1404, 1766, 1780, 1832, 2623
HpySE526I ACGT 3 cut(s) 566, 785, 1846
Hsp92I GRCGYC 3 cut(s) 291, 474, 818
Hsp92II CATG 8 cut(s) 16, 227, 1619, 1751, 1819, 1928, 2078, 2645
HspAI GCGC 6 cut(s) 291, 474, 621, 661, 2099, 2483
KasI GGCGCC 2 cut(s) 290, 473
KpnI GGTACC 1 cut(s) 952
KroI GCCGGC 1 cut(s) 411
KroNI GCCGGC 1 cut(s) 413
Kzo9I GATC 3 cut(s) 550, 1383, 2119
Lsp1109I GCAGC 5 cut(s) 498, 639, 1524, 1707, 2047
LweI GCATC 1 cut(s) 1333
MabI ACCWGGT 1 cut(s) 2113
MaeI CTAG 5 cut(s) 1269, 1434, 1640, 1889, 2583
MaeII ACGT 3 cut(s) 566, 785, 1846
MaeIII GTNAC 5 cut(s) 737, 2422, 2449, 2475, 2601
MalI GATC 3 cut(s) 552, 1385, 2121
MbiI CCGCTC 5 cut(s) 498, 522, 598, 852, 1631
MboI GATC 3 cut(s) 550, 1383, 2119
MboII GAAGA 9 cut(s) 153, 190, 265, 1402, 1506, 1516, 1796, 2610, 2664
MhlI GDGCHC 3 cut(s) 489, 690, 1322
Mly113I GGCGCC 2 cut(s) 291, 474
MlyI GAGTC 9 cut(s) 381, 422, 436, 1067, 1070, 1758, 2313, 2507, 2606
MmeI TCCRAC 4 cut(s) 147, 825, 837, 2412
MroNI GCCGGC 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 2656
MseI TTAA 7 cut(s) 282, 696, 771, 923, 1047, 1599, 2340
MspA1I CMGCKG 4 cut(s) 612, 868, 1019, 1698
MspCI CTTAAG 1 cut(s) 2339
MvaI CCWGG 4 cut(s) 934, 1591, 1692, 2115
MvnI CGCG 3 cut(s) 354, 536, 623
MwoI GCNNNNNNNGC 8 cut(s) 299, 473, 609, 618, 658, 1734, 2060, 2366
NaeI GCCGGC 1 cut(s) 413
NarI GGCGCC 2 cut(s) 291, 474
NciI CCSGG 7 cut(s) 526, 557, 576, 638, 639, 724, 874
NcoI CCATGG 2 cut(s) 12, 223
NdeII GATC 3 cut(s) 550, 1383, 2119
NgoMIV GCCGGC 1 cut(s) 411
NheI GCTAGC 2 cut(s) 1433, 1888
NlaIII CATG 8 cut(s) 16, 227, 1619, 1751, 1819, 1928, 2078, 2645
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 4 cut(s) 737, 2422, 2449, 2601
NspI RCATGY 2 cut(s) 1751, 1819
PaeR7I CTCGAG 1 cut(s) 199
PceI AGGCCT 1 cut(s) 2404
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 414
PdiI GCCGGC 1 cut(s) 413
PdmI GAANNNNTTC 1 cut(s) 2656
PfeI GAWTC 8 cut(s) 55, 181, 370, 583, 1787, 1940, 2246, 2522
PflFI GACNNNGTC 2 cut(s) 817, 2532
PleI GAGTC 9 cut(s) 381, 422, 436, 1067, 1069, 1758, 2313, 2506, 2605
PluTI GGCGCC 2 cut(s) 294, 477
PmaCI CACGTG 1 cut(s) 567
PmlI CACGTG 1 cut(s) 567
PpsI GAGTC 9 cut(s) 381, 422, 436, 1067, 1069, 1758, 2313, 2506, 2605
Ppu21I YACGTR 1 cut(s) 567
PpuMI RGGWCCY 3 cut(s) 255, 325, 628
PshAI GACNNNNGTC 1 cut(s) 725
Psp124BI GAGCTC 2 cut(s) 690, 1322
Psp5II RGGWCCY 3 cut(s) 255, 325, 628
Psp6I CCWGG 4 cut(s) 932, 1589, 1690, 2113
PspCI CACGTG 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 1869
PspGI CCWGG 4 cut(s) 932, 1589, 1690, 2113
PspPPI RGGWCCY 3 cut(s) 255, 325, 628
PstI CTGCAG 1 cut(s) 2215
PsyI GACNNNGTC 2 cut(s) 817, 2532
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 9 cut(s) 191, 311, 713, 806, 950, 1256, 1371, 1660, 1900
RsaNI GTAC 9 cut(s) 190, 310, 712, 805, 949, 1255, 1370, 1659, 1899
SacI GAGCTC 2 cut(s) 690, 1322
SaqAI TTAA 7 cut(s) 282, 696, 771, 923, 1047, 1599, 2340
Sau3AI GATC 3 cut(s) 550, 1383, 2119
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 9 cut(s) 381, 422, 436, 1067, 1070, 1758, 2313, 2507, 2606
SduI GDGCHC 3 cut(s) 489, 690, 1322
SexAI ACCWGGT 1 cut(s) 2113
SfaNI GCATC 1 cut(s) 1333
SfcI CTRYAG 1 cut(s) 2211
SfoI GGCGCC 2 cut(s) 292, 475
Sfr274I CTCGAG 1 cut(s) 199
SinI GGWCC 7 cut(s) 255, 325, 628, 720, 859, 1826, 1856
SlaI CTCGAG 1 cut(s) 199
SmaI CCCGGG 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 199, 2339
SmoI CTYRAG 2 cut(s) 199, 2339
SseBI AGGCCT 1 cut(s) 2404
SspDI GGCGCC 2 cut(s) 290, 473
SspMI CTAG 5 cut(s) 1269, 1434, 1640, 1889, 2583
SstI GAGCTC 2 cut(s) 690, 1322
StuI AGGCCT 1 cut(s) 2404
StyI CCWWGG 3 cut(s) 12, 223, 1639
TaiI ACGT 3 cut(s) 569, 788, 1849
TaqI TCGA 8 cut(s) 176, 200, 390, 440, 549, 1190, 1849, 2540
TatI WGTACW 3 cut(s) 309, 711, 1254
TauI GCSGC 6 cut(s) 291, 467, 501, 868, 2220, 2273
TfiI GAWTC 8 cut(s) 55, 181, 370, 583, 1787, 1940, 2246, 2522
Tru1I TTAA 7 cut(s) 282, 696, 771, 923, 1047, 1599, 2340
Tru9I TTAA 7 cut(s) 282, 696, 771, 923, 1047, 1599, 2340
TscAI CASTG 2 cut(s) 1677, 2667
TseFI GTSAC 4 cut(s) 737, 2422, 2449, 2601
TseI GCWGC 5 cut(s) 511, 652, 1537, 1695, 2060
Tsp45I GTSAC 4 cut(s) 737, 2422, 2449, 2601
TspDTI ATGAA 6 cut(s) 596, 784, 1427, 1517, 2250, 2658
TspMI CCCGGG 1 cut(s) 637
TspRI CASTG 2 cut(s) 1677, 2667
Tth111I GACNNNGTC 2 cut(s) 817, 2532
Vha464I CTTAAG 1 cut(s) 2339
VpaK11BI GGWCC 7 cut(s) 255, 325, 628, 720, 859, 1826, 1856
XagI CCTNNNNNAGG 1 cut(s) 2342
XapI RAATTY 5 cut(s) 1303, 1360, 1549, 1861, 2515
XceI RCATGY 2 cut(s) 1751, 1819
XcmI CCANNNNNNNNNTGG 1 cut(s) 1866
XhoI CTCGAG 1 cut(s) 199
XmaI CCCGGG 1 cut(s) 637
XmaJI CCTAGG 1 cut(s) 1639
XmiI GTMKAC 2 cut(s) 911, 1279
XmnI GAANNNNTTC 1 cut(s) 2656
XspI CTAG 5 cut(s) 1269, 1434, 1640, 1889, 2583
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.