Rh1BG232300

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0011114
Physical Location & Seq
Reverse (-)
35518032 .. 35520722
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG232300.1

Sequence Viewer

Length: 2691 bp
ATGTCTTCTTCCATGGTTCCCATCTCTCTCCTCCTCCCCATACTCCAAACCCTAATCCTCATCTCCACAAACTCCAATCCCATTCCCAAAACTACTTCACCCCAAAACCCAATCCAGCTCCCCAACACCCTTCCATGTCCCACTCCAAACTCAAACGGGTCTTCAAGAACTGTCTCGAAGATTCTTTGGTACGCACCCTTGAGAACCAACAAGGTTATACTCTATGGCAACTCCACCGTTTGCAGTCGCAACAGGACCTTTCAGCTCGGCTTCTTCTCCTCTAATGGCGGCGCCTACTTGGCAATAATGCACTCCTCAATTCAGGTCCCCACCGTAGTCTGGGTCGCCAACCGCGACCGCCCCGTCAAGAATCTCACTTCAGCCAGACTCGAAATCACAGCCACCGACGGCCGGCTCGTCGTTATAGACTCCGATAACTCGACTCTGTGGGCCAGCCAGAACACCGCCGCCGCCGTCGGCGCCCAAGTCGTGCTCCTGGAGAGCGGCAATCTGATGCTGCTCACCCACTCCGGGCAACTCGCGTGGCAGAGCTTCGATCACCCGGCTGACACGTGGCTCCCCGGAATGAATCTGACGGCGGAGCGGGGCCTGACCAGCTGGCGGAGCGCGGAGGACCCGTCCCCGGGTTTGTATTCGCTGCGGTTGCGCCCACAGGATTACGGTGAGTTTGAGCTCGTTTTTAACGGTAGTGTTGAGTACTGGAGGACCGGGGTTTGGGACGGTCAGGCTTTTGCTAATGTGCCGGAGATGGCTGTTAAGTATATCTATACGTTTCACTTCATAGACCCGTACAAGCCGACGGCGTCGTTTTGGTTCACCGAGCGGCCGTCGGAGAGCGGGTTGGACCCGCCGCTGACCCGGTTCCAGATAGACCATACGGGTCAGCTCCGGCAGTATACTTGGGGTTTAAGCGAGACCTGGGACAAGTTTTGGTCCCAGCCTGAGGACAAGTGCTCAGTGGTTGGTTTGTGTGGGAGGTTTGGGGTTTGTGATAGTGAGACTGCTGAACCCTGTGAGTGTCCCTCAGGGTTTAAGCCTGTGGATGAGTCTGGTTGGGACTCTGGGGACTATTCCGGTGGGTGTGAGAGGTTTGGTGATGTGTCTTGTGAGGAAAGAGATGATGGTTTCGTGGAGGTTCGGGTTGAGAGTTTTGCGGAAAGTTGGCATAATAGTTCGATTACTGGTAGGGATGGTTGTGAAAAGATGTGCTTGCAAGTTTGTTCTTGTATTGGTTTTCAGTACAACGAGAGGTCTGGTTTGTGTAGACATTATTTCGGGTCTCTGTTGAATTTGAGGAATGTGAGCTCTGATGGTAATAGCAGTATGGATGCTGGTAGTGTTTTGAATTTGAAGGTACGAAAAGGTGTGATCTTGAAGAACAGGAAGGCTAAGGTTATGAATTATACAGTTTTTGGTGCTAGCATTGTTGGGTCAGTTGCGGTTTTGGGTTTTCTGGTGGTGGTTGTATTGATTAGGAGAAGAAAGATGAAGAGGAATGGAGGAGCAGAGGAAAGTGTTTTGCTGCCGGTGTTGAATTTGAAGGTATTTTCCTACAAAGAGCTTCAGACTGCAACCTGGGGTTTTAAAGAGAAACTCGGGCATGGTGGCTTTGGAGCGGTTTTCCTAGGGGAGCTATCAGACCGTACTCTTGTTGCAGTGAAACGCTTGGAGAGGCCAGGCAGTGGAGAGCAACAATTCCGAGCAGAAGTTTGCACCATTGGCAACATTCAGCATGTCAATCTTGTGAGACTTAGAGGCTTCTGCTCAGAAGATTCTCATAGACTTCTGGTATATGATTACATGCAAAATGGTCCTCTAAGTGTGTATCTACGTCGAGACGGTCCAAATTTGAGCTGGGATGTTAGGTTTCGGCTAGCGGTTGGTACAGCAAGAGGCATTGCATATTTACATGAGGGGTGTAGAGATTCCATAATCCATTGTGATATCAAACCAGAAAACATTTTGCTTGATAGTGATTACACGGCAAAGGTGTCAGATTTTGGGTTAGCAAAGCTAATTCACAGAGACTTTAGTGGAGCATTAGCTGCTGATGGTAATCATGGCTATACCTTTGGCTATGTAGCGCCAGAGTGCAGACCTGGTGATCCAATCACCACAAAAGCCGATGTTTACAGCTATGGAAAGACATTGATAGAATTACTTGGAGGCCATAGAATGTGGGAGGCACCTCCATCTGCAGGCGGCAGAGAGGGTGGAAATGAAATAACAGATTCTTGGTGCTTTCCTTCGTGGGCGGCACAGCAGATAATTGATGGCAATGTGGTGGCTGTAATTGATGATAGACTCGGGAGCAAGTATAACCTTAAGGAGGCCGAGCGTGTTGCATTAGTGGCGGTGTGGTGCATACAGGACGACGAGGCAACAAGGCCTACAATGGGGATGGTAGTGACAATGTTGGAAGGGGTTGTGGAAGTGACTGTTCCTCCAGCACCAAAGTTGTTACAAGCGCTGGTTTCAGGGGAGTCATTCTGTGGGGTGAATTCCGATTCTGGACACAGTCCATCGATTGCCAGCGACAACTTCTCTGGTTGTAATACGAAATTATCTAGTTGCGGTTCAGAGTCGCCACTTGGTAATGTCTCTTCCTTAGTAAATGAGAATGTGCATGAAAACCGAAGAAGTTTTACTGTCAATACTGACCATAGCTAA

Protein Analysis

896

Amino Acids

98.48

Weight (kDa)

5.84

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 111 - 201 3.4e-26 D-mannose binding lectin
S_locus_glycop PF00954 241 - 353 2.9e-22 S-locus glycoprotein domain
Pkinase PF00069 535 - 809 3.2e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 811 9.4e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AccB1I GGYRCC 3 cut(s) 290, 479, 2206
AccB7I CCANNNNNTGG 1 cut(s) 1703
AccBSI CCGCTC 5 cut(s) 504, 604, 844, 858, 1637
AccI GTMKAC 2 cut(s) 917, 1285
AccII CGCG 3 cut(s) 354, 542, 629
AclWI GGATC 1 cut(s) 2120
AcoI YGGCCR 2 cut(s) 409, 845
AcsI RAATTY 5 cut(s) 1309, 1366, 1555, 1867, 2521
AcuI CTGAAG 2 cut(s) 363, 1568
AcvI CACGTG 1 cut(s) 573
AcyI GRCGYC 3 cut(s) 291, 480, 824
AfaI GTAC 7 cut(s) 191, 719, 812, 1262, 1377, 1666, 1906
AfeI AGCGCT 1 cut(s) 2490
AflII CTTAAG 1 cut(s) 2345
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 7 cut(s) 165, 1309, 1366, 1372, 1396, 1555, 1561
AjnI CCWGG 5 cut(s) 495, 938, 1595, 1696, 2119
Alw21I GWGCWC 4 cut(s) 495, 696, 977, 1328
Alw26I GTCTC 8 cut(s) 178, 929, 1013, 1305, 1762, 1851, 2040, 2626
AlwI GGATC 1 cut(s) 2120
Ama87I CYCGRG 3 cut(s) 643, 1616, 2327
Aor51HI AGCGCT 1 cut(s) 2490
AoxI GGCC 8 cut(s) 409, 450, 607, 845, 1694, 2188, 2352, 2408
ApeKI GCWGC 4 cut(s) 517, 658, 1543, 2066
ApoI RAATTY 5 cut(s) 1309, 1366, 1555, 1867, 2521
AspA2I CCTAGG 1 cut(s) 1645
AspLEI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
AsuC2I CCSGG 7 cut(s) 532, 563, 582, 644, 645, 730, 880
AsuHPI GGTGA 9 cut(s) 90, 514, 551, 695, 829, 1127, 2125, 2135, 2530
AsuNHI GCTAGC 2 cut(s) 1439, 1894
AvaI CYCGRG 3 cut(s) 643, 1616, 2327
AvaII GGWCC 8 cut(s) 255, 325, 634, 726, 865, 954, 1832, 1862
AvrII CCTAGG 1 cut(s) 1645
AxyI CCTNAGG 2 cut(s) 963, 1045
BanI GGYRCC 3 cut(s) 290, 479, 2206
BanII GRGCYC 2 cut(s) 696, 1328
BarI GAAGNNNNNNTAC 4 cut(s) 2608, 2640, 2650, 2682
BbrPI CACGTG 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 4 cut(s) 495, 696, 977, 1328
BbvI GCAGC 4 cut(s) 504, 645, 1530, 2053
BceAI ACGGC 6 cut(s) 424, 458, 612, 832, 837, 2019
BcgI CGANNNNNNTGC 2 cut(s) 2345, 2379
BciT130I CCWGG 5 cut(s) 497, 940, 1597, 1698, 2121
BcnI CCSGG 7 cut(s) 532, 563, 582, 644, 645, 730, 880
BcoDI GTCTC 8 cut(s) 178, 929, 1013, 1305, 1762, 1851, 2040, 2626
BfaI CTAG 4 cut(s) 1440, 1646, 1895, 2589
BfmI CTRYAG 1 cut(s) 2217
BfoI RGCGCY 4 cut(s) 294, 483, 2108, 2492
BfrI CTTAAG 1 cut(s) 2345
BglI GCCNNNNNGGC 1 cut(s) 299
BlnI CCTAGG 1 cut(s) 1645
BmcAI AGTACT 1 cut(s) 719
Bme18I GGWCC 8 cut(s) 255, 325, 634, 726, 865, 954, 1832, 1862
BmeT110I CYCGRG 3 cut(s) 643, 1616, 2327
BmsI GCATC 2 cut(s) 504, 1339
BmtI GCTAGC 2 cut(s) 1443, 1898
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 3 cut(s) 518, 742, 2451
Bpu10I CCTNAGC 1 cut(s) 1410
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 7 cut(s) 532, 563, 582, 644, 645, 730, 880
Bsa29I ATCGAT 1 cut(s) 2546
BsaAI YACGTR 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 2076
BsaHI GRCGYC 3 cut(s) 291, 480, 824
BsaI GGTCTC 2 cut(s) 929, 1305
BsaJI CCNNGG 8 cut(s) 12, 580, 642, 643, 729, 939, 1596, 1645
BsaWI WCCGGW 1 cut(s) 1094
BsaXI ACNNNNNCTCC 6 cut(s) 623, 653, 845, 875, 2449, 2479
Bse118I RCCGGY 2 cut(s) 411, 1546
Bse1I ACTGG 2 cut(s) 725, 1207
Bse21I CCTNAGG 2 cut(s) 963, 1045
Bse3DI GCAATG 2 cut(s) 1917, 2305
Bse8I GATNNNNATC 1 cut(s) 2076
BseBI CCWGG 5 cut(s) 497, 940, 1597, 1698, 2121
BseCI ATCGAT 1 cut(s) 2546
BseDI CCNNGG 8 cut(s) 12, 580, 642, 643, 729, 939, 1596, 1645
BseGI GGATG 5 cut(s) 1069, 1216, 1354, 1885, 2427
BseJI GATNNNNATC 1 cut(s) 2076
BseMI GCAATG 2 cut(s) 1917, 2305
BseMII CTCAG 4 cut(s) 954, 990, 1059, 1800
BseNI ACTGG 2 cut(s) 725, 1207
BseRI GAGGAG 5 cut(s) 20, 23, 268, 304, 1536
BseX3I CGGCCG 2 cut(s) 409, 845
BseXI GCAGC 4 cut(s) 504, 645, 1530, 2053
BseYI CCCAGC 2 cut(s) 957, 1875
BsgI GTGCAG 1 cut(s) 2134
Bsh1236I CGCG 3 cut(s) 354, 542, 629
Bsh1285I CGRYCG 3 cut(s) 358, 412, 848
BshFI GGCC 8 cut(s) 411, 452, 609, 847, 1696, 2190, 2354, 2410
BshNI GGYRCC 3 cut(s) 290, 479, 2206
BshVI ATCGAT 1 cut(s) 2546
BsiEI CGRYCG 3 cut(s) 358, 412, 848
BsiHKAI GWGCWC 4 cut(s) 495, 696, 977, 1328
BsiHKCI CYCGRG 3 cut(s) 643, 1616, 2327
BslFI GGGAC 9 cut(s) 123, 311, 625, 752, 940, 956, 1026, 1091, 1100
BsmAI GTCTC 8 cut(s) 178, 929, 1013, 1305, 1762, 1851, 2040, 2626
BsmBI CGTCTC 1 cut(s) 1851
BsmFI GGGAC 9 cut(s) 123, 311, 625, 752, 940, 956, 1026, 1091, 1100
BsnI GGCC 8 cut(s) 411, 452, 609, 847, 1696, 2190, 2354, 2410
Bso31I GGTCTC 2 cut(s) 929, 1305
BsoBI CYCGRG 3 cut(s) 643, 1616, 2327
Bsp1286I GDGCHC 4 cut(s) 495, 696, 977, 1328
Bsp143I GATC 3 cut(s) 556, 1389, 2125
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 8 cut(s) 411, 452, 609, 847, 1696, 2190, 2354, 2410
BspCNI CTCAG 4 cut(s) 955, 989, 1058, 1799
BspDI ATCGAT 1 cut(s) 2546
BspFNI CGCG 3 cut(s) 354, 542, 629
BspMAI CTGCAG 1 cut(s) 2221
BspOI GCTAGC 2 cut(s) 1443, 1898
BspPI GGATC 1 cut(s) 2120
BspT107I GGYRCC 3 cut(s) 290, 479, 2206
BspTI CTTAAG 1 cut(s) 2345
BspTNI GGTCTC 2 cut(s) 929, 1305
BsrBI CCGCTC 5 cut(s) 504, 604, 844, 858, 1637
BsrDI GCAATG 2 cut(s) 1917, 2305
BsrFI RCCGGY 2 cut(s) 411, 1546
BsrI ACTGG 2 cut(s) 725, 1207
BssAI RCCGGY 2 cut(s) 411, 1546
BssECI CCNNGG 8 cut(s) 12, 580, 642, 643, 729, 939, 1596, 1645
BssMI GATC 3 cut(s) 556, 1389, 2125
BssNAI GTATAC 1 cut(s) 918
BssNI GRCGYC 3 cut(s) 291, 480, 824
BssT1I CCWWGG 2 cut(s) 12, 1645
Bst1107I GTATAC 1 cut(s) 918
Bst2UI CCWGG 5 cut(s) 497, 940, 1597, 1698, 2121
Bst6I CTCTTC 2 cut(s) 1505, 2629
BstACI GRCGYC 3 cut(s) 291, 480, 824
BstAFI CTTAAG 1 cut(s) 2345
BstAPI GCANNNNNTGC 1 cut(s) 2066
BstBAI YACGTR 1 cut(s) 573
BstC8I GCNNGC 8 cut(s) 413, 454, 620, 1232, 1441, 1896, 2221, 2554
BstDEI CTNAG 8 cut(s) 963, 976, 1045, 1410, 1772, 1786, 1838, 2629
BstDSI CCRYGG 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 2348
BstF5I GGATG 5 cut(s) 1069, 1216, 1354, 1885, 2427
BstFNI CGCG 3 cut(s) 354, 542, 629
BstH2I RGCGCY 4 cut(s) 294, 483, 2108, 2492
BstHHI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
BstKTI GATC 3 cut(s) 559, 1392, 2128
BstMAI GTCTC 8 cut(s) 178, 929, 1013, 1305, 1762, 1851, 2040, 2626
BstMBI GATC 3 cut(s) 556, 1389, 2125
BstMCI CGRYCG 3 cut(s) 358, 412, 848
BstMWI GCNNNNNNNGC 8 cut(s) 299, 479, 615, 624, 664, 1740, 2066, 2372
BstNI CCWGG 5 cut(s) 497, 940, 1597, 1698, 2121
BstNSI RCATGY 2 cut(s) 1757, 1825
BstSFI CTRYAG 1 cut(s) 2217
BstUI CGCG 3 cut(s) 354, 542, 629
BstV1I GCAGC 4 cut(s) 504, 645, 1530, 2053
BstV2I GAAGAC 1 cut(s) 153
BstZ17I GTATAC 1 cut(s) 918
BstZI CGGCCG 2 cut(s) 409, 845
Bsu15I ATCGAT 1 cut(s) 2546
Bsu36I CCTNAGG 2 cut(s) 963, 1045
BsuRI GGCC 8 cut(s) 411, 452, 609, 847, 1696, 2190, 2354, 2410
BsuTUI ATCGAT 1 cut(s) 2546
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 5 cut(s) 1069, 1216, 1354, 1885, 2427
BtsI GCAGTG 2 cut(s) 1683, 1708
BtsIMutI CAGTG 3 cut(s) 984, 1683, 1708
Cac8I GCNNGC 8 cut(s) 413, 454, 620, 1232, 1441, 1896, 2221, 2554
CfoI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
Cfr10I RCCGGY 2 cut(s) 411, 1546
Cfr9I CCCGGG 1 cut(s) 643
ClaI ATCGAT 1 cut(s) 2546
CseI GACGC 1 cut(s) 813
CsiI ACCWGGT 1 cut(s) 2119
Csp6I GTAC 7 cut(s) 190, 718, 811, 1261, 1376, 1665, 1905
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CviAII CATG 8 cut(s) 13, 135, 1622, 1754, 1822, 1931, 2081, 2648
CviQI GTAC 7 cut(s) 190, 718, 811, 1261, 1376, 1665, 1905
DdeI CTNAG 8 cut(s) 963, 976, 1045, 1410, 1772, 1786, 1838, 2629
DinI GGCGCC 2 cut(s) 292, 481
DpnI GATC 3 cut(s) 558, 1391, 2127
DpnII GATC 3 cut(s) 556, 1389, 2125
DraI TTTAAA 1 cut(s) 1606
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 2 cut(s) 409, 845
EagI CGGCCG 2 cut(s) 409, 845
Eam1104I CTCTTC 2 cut(s) 1505, 2629
EarI CTCTTC 2 cut(s) 1505, 2629
EciI GGCGGA 2 cut(s) 614, 637
Ecl136II GAGCTC 2 cut(s) 694, 1326
EclXI CGGCCG 2 cut(s) 409, 845
Eco130I CCWWGG 2 cut(s) 12, 1645
Eco147I AGGCCT 1 cut(s) 2410
Eco24I GRGCYC 2 cut(s) 696, 1328
Eco31I GGTCTC 2 cut(s) 929, 1305
Eco32I GATATC 1 cut(s) 1966
Eco47I GGWCC 8 cut(s) 255, 325, 634, 726, 865, 954, 1832, 1862
Eco47III AGCGCT 1 cut(s) 2490
Eco52I CGGCCG 2 cut(s) 409, 845
Eco53kI GAGCTC 2 cut(s) 694, 1326
Eco57I CTGAAG 2 cut(s) 363, 1568
Eco72I CACGTG 1 cut(s) 573
Eco81I CCTNAGG 2 cut(s) 963, 1045
Eco88I CYCGRG 3 cut(s) 643, 1616, 2327
EcoICRI GAGCTC 2 cut(s) 694, 1326
EcoNI CCTNNNNNAGG 1 cut(s) 2348
EcoO109I RGGNCCY 4 cut(s) 255, 325, 607, 634
EcoRI GAATTC 1 cut(s) 2521
EcoRII CCWGG 5 cut(s) 495, 938, 1595, 1696, 2119
EcoRV GATATC 1 cut(s) 1966
EcoT14I CCWWGG 2 cut(s) 12, 1645
EcoT38I GRGCYC 2 cut(s) 696, 1328
EgeI GGCGCC 2 cut(s) 292, 481
EheI GGCGCC 2 cut(s) 292, 481
ErhI CCWWGG 2 cut(s) 12, 1645
Esp3I CGTCTC 1 cut(s) 1851
FaeI CATG 8 cut(s) 16, 138, 1625, 1757, 1825, 1934, 2084, 2651
FalI AAGNNNNNCTT 2 cut(s) 1214, 1246
FaqI GGGAC 9 cut(s) 123, 311, 625, 752, 940, 956, 1026, 1091, 1100
FatI CATG 8 cut(s) 12, 134, 1621, 1753, 1821, 1930, 2080, 2647
FauI CCCGC 3 cut(s) 597, 851, 876
FblI GTMKAC 2 cut(s) 917, 1285
FokI GGATG 5 cut(s) 1076, 1223, 1361, 1892, 2434
FriOI GRGCYC 2 cut(s) 696, 1328
FspBI CTAG 4 cut(s) 1440, 1646, 1895, 2589
GlaI GCGC 6 cut(s) 292, 481, 628, 668, 2106, 2490
GsaI CCCAGC 2 cut(s) 961, 1879
GsuI CTGGAG 3 cut(s) 518, 742, 2451
HaeII RGCGCY 4 cut(s) 294, 483, 2108, 2492
HaeIII GGCC 8 cut(s) 411, 452, 609, 847, 1696, 2190, 2354, 2410
HgaI GACGC 1 cut(s) 813
HhaI GCGC 6 cut(s) 293, 482, 629, 669, 2107, 2491
Hin1I GRCGYC 3 cut(s) 291, 480, 824
Hin1II CATG 8 cut(s) 16, 138, 1625, 1757, 1825, 1934, 2084, 2651
Hin6I GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
HinP1I GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
HphI GGTGA 9 cut(s) 90, 514, 551, 695, 829, 1127, 2125, 2135, 2530
Hpy166II GTNNAC 4 cut(s) 837, 918, 1286, 2152
Hpy188III TCNNGA 8 cut(s) 165, 175, 367, 886, 1393, 1856, 2329, 2532
Hpy8I GTNNAC 4 cut(s) 837, 918, 1286, 2152
Hpy99I CGWCG 8 cut(s) 410, 422, 479, 823, 829, 853, 1857, 2399
HpyAV CCTTC 6 cut(s) 140, 1366, 1399, 1555, 2277, 2435
HpyCH4IV ACGT 3 cut(s) 572, 791, 1852
HpyF10VI GCNNNNNNNGC 8 cut(s) 299, 479, 615, 624, 664, 1740, 2066, 2372
HpyF3I CTNAG 8 cut(s) 963, 976, 1045, 1410, 1772, 1786, 1838, 2629
HpySE526I ACGT 3 cut(s) 572, 791, 1852
Hsp92I GRCGYC 3 cut(s) 291, 480, 824
Hsp92II CATG 8 cut(s) 16, 138, 1625, 1757, 1825, 1934, 2084, 2651
HspAI GCGC 6 cut(s) 291, 480, 627, 667, 2105, 2489
KasI GGCGCC 2 cut(s) 290, 479
KroI GCCGGC 1 cut(s) 411
KroNI GCCGGC 1 cut(s) 413
Kzo9I GATC 3 cut(s) 556, 1389, 2125
Lsp1109I GCAGC 4 cut(s) 504, 645, 1530, 2053
LweI GCATC 2 cut(s) 504, 1339
MabI ACCWGGT 1 cut(s) 2119
MaeI CTAG 4 cut(s) 1440, 1646, 1895, 2589
MaeII ACGT 3 cut(s) 572, 791, 1852
MaeIII GTNAC 3 cut(s) 2428, 2455, 2481
MalI GATC 3 cut(s) 558, 1391, 2127
MbiI CCGCTC 5 cut(s) 504, 604, 844, 858, 1637
MboI GATC 3 cut(s) 556, 1389, 2125
MboII GAAGA 9 cut(s) 153, 190, 265, 1408, 1512, 1522, 1802, 2616, 2670
MhlI GDGCHC 4 cut(s) 495, 696, 977, 1328
Mly113I GGCGCC 2 cut(s) 291, 480
MlyI GAGTC 8 cut(s) 381, 422, 436, 1073, 1076, 2319, 2513, 2612
MmeI TCCRAC 3 cut(s) 831, 843, 2418
MroNI GCCGGC 1 cut(s) 411
MseI TTAA 6 cut(s) 702, 777, 929, 1053, 1605, 2346
MspA1I CMGCKG 2 cut(s) 618, 874
MspCI CTTAAG 1 cut(s) 2345
MvaI CCWGG 5 cut(s) 497, 940, 1597, 1698, 2121
MvnI CGCG 3 cut(s) 354, 542, 629
MwoI GCNNNNNNNGC 8 cut(s) 299, 479, 615, 624, 664, 1740, 2066, 2372
NaeI GCCGGC 1 cut(s) 413
NarI GGCGCC 2 cut(s) 291, 480
NciI CCSGG 7 cut(s) 532, 563, 582, 644, 645, 730, 880
NcoI CCATGG 1 cut(s) 12
NdeII GATC 3 cut(s) 556, 1389, 2125
NgoMIV GCCGGC 1 cut(s) 411
NheI GCTAGC 2 cut(s) 1439, 1894
NlaIII CATG 8 cut(s) 16, 138, 1625, 1757, 1825, 1934, 2084, 2651
NmeAIII GCCGAG 2 cut(s) 246, 2380
NmuCI GTSAC 2 cut(s) 2428, 2455
NspI RCATGY 2 cut(s) 1757, 1825
PceI AGGCCT 1 cut(s) 2410
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 414
PdiI GCCGGC 1 cut(s) 413
PfeI GAWTC 7 cut(s) 181, 370, 589, 1793, 1946, 2252, 2528
PflFI GACNNNGTC 2 cut(s) 823, 2538
PflMI CCANNNNNTGG 1 cut(s) 1703
PfoI TCCNGGA 1 cut(s) 495
PleI GAGTC 8 cut(s) 381, 422, 436, 1073, 1075, 2319, 2512, 2611
PluTI GGCGCC 2 cut(s) 294, 483
PmaCI CACGTG 1 cut(s) 573
PmlI CACGTG 1 cut(s) 573
PpsI GAGTC 8 cut(s) 381, 422, 436, 1073, 1075, 2319, 2512, 2611
Ppu21I YACGTR 1 cut(s) 573
PpuMI RGGWCCY 3 cut(s) 255, 325, 634
Psp124BI GAGCTC 2 cut(s) 696, 1328
Psp5II RGGWCCY 3 cut(s) 255, 325, 634
Psp6I CCWGG 5 cut(s) 495, 938, 1595, 1696, 2119
PspCI CACGTG 1 cut(s) 573
PspFI CCCAGC 2 cut(s) 957, 1875
PspGI CCWGG 5 cut(s) 495, 938, 1595, 1696, 2119
PspPPI RGGWCCY 3 cut(s) 255, 325, 634
PstI CTGCAG 1 cut(s) 2221
PsyI GACNNNGTC 2 cut(s) 823, 2538
PvuII CAGCTG 1 cut(s) 618
RsaI GTAC 7 cut(s) 191, 719, 812, 1262, 1377, 1666, 1906
RsaNI GTAC 7 cut(s) 190, 718, 811, 1261, 1376, 1665, 1905
SacI GAGCTC 2 cut(s) 696, 1328
SaqAI TTAA 6 cut(s) 702, 777, 929, 1053, 1605, 2346
Sau3AI GATC 3 cut(s) 556, 1389, 2125
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 8 cut(s) 381, 422, 436, 1073, 1076, 2319, 2513, 2612
SduI GDGCHC 4 cut(s) 495, 696, 977, 1328
SexAI ACCWGGT 1 cut(s) 2119
SfaNI GCATC 2 cut(s) 504, 1339
SfcI CTRYAG 1 cut(s) 2217
SfoI GGCGCC 2 cut(s) 292, 481
SinI GGWCC 8 cut(s) 255, 325, 634, 726, 865, 954, 1832, 1862
SmaI CCCGGG 1 cut(s) 645
SmlI CTYRAG 2 cut(s) 199, 2345
SmoI CTYRAG 2 cut(s) 199, 2345
SseBI AGGCCT 1 cut(s) 2410
SspDI GGCGCC 2 cut(s) 290, 479
SspMI CTAG 4 cut(s) 1440, 1646, 1895, 2589
SstI GAGCTC 2 cut(s) 696, 1328
StuI AGGCCT 1 cut(s) 2410
StyI CCWWGG 2 cut(s) 12, 1645
TaiI ACGT 3 cut(s) 575, 794, 1855
TaqI TCGA 7 cut(s) 176, 390, 440, 555, 1196, 1855, 2546
TatI WGTACW 2 cut(s) 717, 1260
TauI GCSGC 8 cut(s) 291, 470, 473, 507, 847, 874, 2226, 2279
TfiI GAWTC 7 cut(s) 181, 370, 589, 1793, 1946, 2252, 2528
Tru1I TTAA 6 cut(s) 702, 777, 929, 1053, 1605, 2346
Tru9I TTAA 6 cut(s) 702, 777, 929, 1053, 1605, 2346
TscAI CASTG 3 cut(s) 984, 1683, 1708
TseFI GTSAC 2 cut(s) 2428, 2455
TseI GCWGC 4 cut(s) 517, 658, 1543, 2066
Tsp45I GTSAC 2 cut(s) 2428, 2455
TspDTI ATGAA 6 cut(s) 602, 790, 1433, 1523, 2256, 2664
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 3 cut(s) 984, 1683, 1708
Tth111I GACNNNGTC 2 cut(s) 823, 2538
Van91I CCANNNNNTGG 1 cut(s) 1703
Vha464I CTTAAG 1 cut(s) 2345
VpaK11BI GGWCC 8 cut(s) 255, 325, 634, 726, 865, 954, 1832, 1862
XagI CCTNNNNNAGG 1 cut(s) 2348
XapI RAATTY 5 cut(s) 1309, 1366, 1555, 1867, 2521
XceI RCATGY 2 cut(s) 1757, 1825
XcmI CCANNNNNNNNNTGG 1 cut(s) 1872
XmaI CCCGGG 1 cut(s) 643
XmaJI CCTAGG 1 cut(s) 1645
XmiI GTMKAC 2 cut(s) 917, 1285
XspI CTAG 4 cut(s) 1440, 1646, 1895, 2589
ZrmI AGTACT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.