MD01G1073100.v1.1

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016087
Physical Location & Seq
Reverse (-)
17820015 .. 17822548
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1073100.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGTACTTCTCATTCCTTCGCCGGAAATATCCAGAGTTTTCTGCAACCCTAATCCCAATCTCAAAAACTTTAAAATCTCCACGAACATTGATAAATTTGGGTTTGGTTCTAGGGTTAGGGTTTCGTCCAAGGTTAGGGATTGTACGGTTAGGGTTTCGGAATTCGACCAAAATGTGAGGCTGTACGGACAGTTTTCGGCTCCTGTGAAGCGAGGAGGTTCGAAGCCGAGCAAAGAAGAGGAAGAGAAGCAGGATTACTATGTGAATATGGGGTATGCGATTCGGACTCTGAGGGAGGAATTTCCTCAGCTGTTTTACAGAGAACTCAGCTTCGACATTTACAGGGATGATATCACTTTCAAAGATCCAATGAATACCTTTATGGGCATTGAGAATTATAAGTCTATATATTGGGCACTGCGTTTCCATGGTCAAATATTTTTCAAGGCCTTGTGGGTTGATGTCATTAGTGTATGGCAGCCCATGGACAATGTAATCATGGTTCGCTGGACCATCCATGGCATTCCACGAGTCCCTTGGGATAGTCGTGGTCGATTTGATGGAACCTCTGAGTACAAACTTGACAAGCAAGGGAAGATCTTTGAGCATCGGGTTGATAACATTGCTCTCAATTCTCCACCTCCAAAGTTTCAAGTGCTGACCGTAGTGGATATAATGCAATCTCTCGGATGCCCCTCAACCCCAAGGCCAACATATTTCAAAATTTCATCTTCTTTGTCTTCAAAGAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

252

Amino Acids

29.28

Weight (kDa)

9.43

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 93 - 203 3.4e-40 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46220
fragaria_vesca FvH4_7g15580
malus_domestica MD01G1073100.v1.1
prunus_persica Prupe.2G179500_v2.0.a1
pyrus_communis pycom01g10210
rosa_chinensis RchiOBHm_Chr1g0358261
rosa_laevigata RLG00000027979
rosa_multiflora Rmu_co8490635.1_g000001 Rmu_sc0002071.1_g000013
rosa_roxburghii Rroxscaffold_4G00298320
rosa_rugosa Rorug01G0259900
rosa_samantha Rh1BG241100 Rh1CG257600 Rh1DG269800
rosa_wichuraiana Rw1G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 4 cut(s) 95, 161, 299, 723
AfaI GTAC 4 cut(s) 6, 145, 185, 575
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 5 cut(s) 361, 445, 653, 721, 744
AluBI AGCT 2 cut(s) 310, 330
AluI AGCT 2 cut(s) 310, 330
AlwI GGATC 1 cut(s) 359
AoxI GGCC 2 cut(s) 447, 707
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 4 cut(s) 95, 161, 299, 723
AspS9I GGNCC 1 cut(s) 510
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 1 cut(s) 510
BaeGI GKGCMC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 732
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 521, 554
BfaI CTAG 1 cut(s) 111
BglII AGATCT 1 cut(s) 597
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
Bme18I GGWCC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 201, 565
BmsI GCATC 2 cut(s) 616, 680
BpiI GAAGAC 1 cut(s) 732
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 221
BsaJI CCNNGG 6 cut(s) 129, 427, 483, 517, 536, 704
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 621
BseDI CCNNGG 6 cut(s) 129, 427, 483, 517, 536, 704
BseGI GGATG 3 cut(s) 352, 513, 695
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 4 cut(s) 281, 320, 340, 561
BseRI GAGGAG 1 cut(s) 228
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 1 cut(s) 490
BshFI GGCC 2 cut(s) 449, 709
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 522
BsnI GGCC 2 cut(s) 449, 709
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 2 cut(s) 364, 597
Bsp19I CCATGG 3 cut(s) 427, 483, 517
BspANI GGCC 2 cut(s) 449, 709
BspCNI CTCAG 4 cut(s) 282, 319, 339, 562
BspLI GGNNCC 2 cut(s) 201, 565
BspPI GGATC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 621
BssECI CCNNGG 6 cut(s) 129, 427, 483, 517, 536, 704
BssMI GATC 2 cut(s) 364, 597
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 6 cut(s) 129, 427, 483, 517, 536, 704
Bst2BI CACGAG 1 cut(s) 528
Bst4CI ACNGT 3 cut(s) 148, 192, 664
Bst6I CTCTTC 2 cut(s) 231, 237
BstBI TTCGAA 1 cut(s) 221
BstDEI CTNAG 4 cut(s) 290, 306, 326, 570
BstDSI CCRYGG 3 cut(s) 427, 483, 517
BstF5I GGATG 3 cut(s) 352, 513, 695
BstKTI GATC 2 cut(s) 367, 600
BstMBI GATC 2 cut(s) 364, 597
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 2 cut(s) 364, 597
BstYI RGATCY 2 cut(s) 364, 597
BsuRI GGCC 2 cut(s) 449, 709
BtgI CCRYGG 3 cut(s) 427, 483, 517
BtsCI GGATG 3 cut(s) 352, 513, 695
BtsI GCAGTG 1 cut(s) 416
BtsIMutI CAGTG 1 cut(s) 416
Cfr13I GGNCC 1 cut(s) 510
Csp6I GTAC 4 cut(s) 5, 144, 184, 574
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 4 cut(s) 428, 484, 499, 518
CviJI RGCY 8 cut(s) 181, 200, 226, 310, 330, 449, 481, 709
CviKI_1 RGCY 8 cut(s) 181, 200, 226, 310, 330, 449, 481, 709
CviQI GTAC 4 cut(s) 5, 144, 184, 574
DdeI CTNAG 4 cut(s) 290, 306, 326, 570
DpnI GATC 2 cut(s) 366, 599
DpnII GATC 2 cut(s) 364, 597
DraI TTTAAA 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 231, 237
EarI CTCTTC 2 cut(s) 231, 237
Eco130I CCWWGG 6 cut(s) 129, 427, 483, 517, 536, 704
Eco147I AGGCCT 1 cut(s) 449
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 510
EcoRI GAATTC 1 cut(s) 161
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 6 cut(s) 129, 427, 483, 517, 536, 704
ErhI CCWWGG 6 cut(s) 129, 427, 483, 517, 536, 704
FaeI CATG 4 cut(s) 431, 487, 502, 521
FaqI GGGAC 1 cut(s) 518
FatI CATG 4 cut(s) 427, 483, 498, 517
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 3 cut(s) 359, 500, 702
Fsp4HI GCNGC 1 cut(s) 479
FspBI CTAG 1 cut(s) 111
GluI GCNGC 1 cut(s) 479
HaeIII GGCC 2 cut(s) 449, 709
HapII CCGG 1 cut(s) 23
Hin1II CATG 4 cut(s) 431, 487, 502, 521
HinfI GANTC 3 cut(s) 280, 286, 531
HpaII CCGG 1 cut(s) 23
Hpy188I TCNGA 5 cut(s) 160, 285, 291, 571, 689
Hpy188III TCNNGA 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 27
HpyCH4III ACNGT 3 cut(s) 148, 192, 664
HpyCH4V TGCA 2 cut(s) 45, 679
HpyF3I CTNAG 4 cut(s) 290, 306, 326, 570
Hsp92II CATG 4 cut(s) 431, 487, 502, 521
Kzo9I GATC 2 cut(s) 364, 597
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 6 cut(s) 36, 46, 216, 236, 328, 493
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 2 cut(s) 616, 680
MaeI CTAG 1 cut(s) 111
MalI GATC 2 cut(s) 366, 599
MboI GATC 2 cut(s) 364, 597
MboII GAAGA 5 cut(s) 248, 254, 607, 723, 732
MflI RGATCY 2 cut(s) 364, 597
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 6 cut(s) 95, 161, 299, 394, 631, 723
MlyI GAGTC 2 cut(s) 280, 540
MseI TTAA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 23
Mva1269I GAATGC 1 cut(s) 522
NcoI CCATGG 3 cut(s) 427, 483, 517
NdeII GATC 2 cut(s) 364, 597
NlaIII CATG 4 cut(s) 431, 487, 502, 521
NlaIV GGNNCC 2 cut(s) 201, 565
NmeAIII GCCGAG 1 cut(s) 252
NspV TTCGAA 1 cut(s) 221
PceI AGGCCT 1 cut(s) 449
PctI GAATGC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 2 cut(s) 280, 539
PpsI GAGTC 2 cut(s) 280, 539
PsiI TTATAA 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 201, 565
PspPI GGNCC 1 cut(s) 510
PsrI GAACNNNNNNTAC 2 cut(s) 486, 518
PsuI RGATCY 2 cut(s) 364, 597
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 4 cut(s) 6, 145, 185, 575
RsaNI GTAC 4 cut(s) 5, 144, 184, 574
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 2 cut(s) 364, 597
Sau96I GGNCC 1 cut(s) 510
SchI GAGTC 2 cut(s) 280, 540
SduI GDGCHC 1 cut(s) 418
SetI ASST 7 cut(s) 135, 220, 312, 332, 380, 569, 643
SfaNI GCATC 2 cut(s) 616, 680
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 1 cut(s) 510
Sse9I AATT 6 cut(s) 95, 161, 299, 394, 631, 723
SseBI AGGCCT 1 cut(s) 449
SspI AATATT 1 cut(s) 438
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 449
StyI CCWWGG 6 cut(s) 129, 427, 483, 517, 536, 704
TaaI ACNGT 3 cut(s) 148, 192, 664
TaqI TCGA 4 cut(s) 165, 221, 333, 553
TasI AATT 6 cut(s) 95, 161, 299, 394, 631, 723
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 423
TseI GCWGC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 386, 717
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 4 cut(s) 95, 161, 299, 723
XcmI CCANNNNNNNNNTGG 1 cut(s) 534
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.