Rw1G024490

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016087
Physical Location & Seq
Reverse (-)
50658973 .. 50661040
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024490.1

Sequence Viewer

Length: 747 bp
ATGGCTCTTCTCGTTCCGTCACCGGAAATCTCCATAGTCTTCTCTAACCCTAACCCTAACCGTAAAGGCTTTCGAATTCCGACGAACTTCGGGTCGAAATCCAGGTCCACGGTTAGGGTTTGCGCCGGGGTTAAAGACAGTGCAGTGAGCGCGTCGGAATTGGATCGGAATTTAAGGTCGTACGGGCAGTTCTCGGCTCCGGTGAAGCAGGGTCCGAAGCCGAGCAAGGAAGATCAAGAGAGGCAGGAGTACTATGTGAATATGGGGTATGCGATTCGGACTCTGAGGGAGGAGTTTCCCGAGCTGTTTTTCAGAGAGCTTAGCCTCGACATTTACAGGGATGATATCACATTCAAAGATCCGATCAATACCTTTATGGGCATTGAGAATTACAAGTCTATATTTTCGGCACTACGTTTCCATGGCCAGATATTTTTCAAGGCCTTGTGGGTTGACGTCATTAGTGTATCGCAGCCGATGGACAATGTTATAATGGTTCGCTGGACTATCCATGGCATGCCACGAGTCCCTTGGGAAAGTCGTGGTCGATTTGATGGAACCTCAGAGTACAAACTTGACAAGAAAGGGAAGATCTATGAGCATCGTGTCGATAACATTGCCCTGAATTCGCCTCCAAAGTTTCACATTCTGGCCGTAGGCGATATAATGCAATCTCTTGGGTGCCCTTCAACTCCAAAGCCAACATTTTTTGAAACTTCATCATTGCCATCTTCAAAGAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

248

Amino Acids

28.3

Weight (kDa)

9.37

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 91 - 201 2.6e-39 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46220
fragaria_vesca FvH4_7g15580
malus_domestica MD01G1073100.v1.1
prunus_persica Prupe.2G179500_v2.0.a1
pyrus_communis pycom01g10210
rosa_chinensis RchiOBHm_Chr1g0358261
rosa_laevigata RLG00000027979
rosa_multiflora Rmu_co8490635.1_g000001 Rmu_sc0002071.1_g000013
rosa_roxburghii Rroxscaffold_4G00298320
rosa_rugosa Rorug01G0259900
rosa_samantha Rh1BG241100 Rh1CG257600 Rh1DG269800
rosa_wichuraiana Rw1G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
AatII GACGTC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 681
AccII CGCG 1 cut(s) 152
AclWI GGATC 2 cut(s) 171, 353
AcoI YGGCCR 2 cut(s) 424, 651
AcsI RAATTY 3 cut(s) 75, 169, 625
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 3 cut(s) 182, 251, 569
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 5 cut(s) 355, 439, 690, 713, 735
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 304, 319
AluI AGCT 2 cut(s) 304, 319
AlwI GGATC 2 cut(s) 171, 353
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 424, 441, 651
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 3 cut(s) 75, 169, 625
ArsI GACNNNNNNTTYG 4 cut(s) 77, 89, 109, 121
AspLEI GCGC 2 cut(s) 125, 152
AspS9I GGNCC 2 cut(s) 105, 212
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 2 cut(s) 12, 214
AsuII TTCGAA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 105, 212
BaeGI GKGCMC 1 cut(s) 686
BalI TGGCCA 1 cut(s) 426
BanI GGYRCC 1 cut(s) 681
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 484
BccI CCATC 3 cut(s) 472, 548, 736
BceAI ACGGC 1 cut(s) 638
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 127
BglII AGATCT 1 cut(s) 591
BisI GCNGC 1 cut(s) 473
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 474
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 103, 127
Bme18I GGWCC 2 cut(s) 105, 212
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 105, 212
BmiI GGNNCC 4 cut(s) 198, 213, 559, 683
BmrFI CCNGG 2 cut(s) 103, 127
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BsaWI WCCGGW 2 cut(s) 22, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse3DI GCAATG 2 cut(s) 615, 722
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 2 cut(s) 615, 722
BseMII CTCAG 2 cut(s) 275, 576
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 686
BseXI GCAGC 1 cut(s) 484
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 3 cut(s) 426, 443, 653
BshNI GGYRCC 1 cut(s) 681
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 23, 126, 200
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 3 cut(s) 426, 443, 653
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 686
Bsp143I GATC 5 cut(s) 163, 232, 358, 363, 591
Bsp1720I GCTNAGC 1 cut(s) 320
Bsp19I CCATGG 2 cut(s) 421, 511
BspANI GGCC 3 cut(s) 426, 443, 653
BspCNI CTCAG 2 cut(s) 276, 575
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 4 cut(s) 198, 213, 559, 683
BspPI GGATC 2 cut(s) 171, 353
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 681
BsrDI GCAATG 2 cut(s) 615, 722
BssECI CCNNGG 5 cut(s) 108, 126, 421, 511, 530
BssMI GATC 5 cut(s) 163, 232, 358, 363, 591
BssNI GRCGYC 1 cut(s) 456
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 421, 511, 530
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 3 cut(s) 62, 112, 140
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 518
BstDEI CTNAG 3 cut(s) 284, 320, 562
BstDSI CCRYGG 3 cut(s) 108, 421, 511
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 2 cut(s) 125, 152
BstKTI GATC 5 cut(s) 166, 235, 361, 366, 594
BstMBI GATC 5 cut(s) 163, 232, 358, 363, 591
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 2 cut(s) 101, 125
BstSLI GKGCMC 1 cut(s) 686
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 358, 591
BstYI RGATCY 2 cut(s) 358, 591
BsuRI GGCC 3 cut(s) 426, 443, 653
BtgI CCRYGG 3 cut(s) 108, 421, 511
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 145, 150
Cac8I GCNNGC 1 cut(s) 518
CfoI GCGC 2 cut(s) 125, 152
Cfr13I GGNCC 2 cut(s) 105, 212
CseI GACGC 1 cut(s) 141
Csp6I GTAC 3 cut(s) 181, 250, 568
CviAII CATG 3 cut(s) 422, 512, 517
CviQI GTAC 3 cut(s) 181, 250, 568
DdeI CTNAG 3 cut(s) 284, 320, 562
DpnI GATC 5 cut(s) 165, 234, 360, 365, 593
DpnII GATC 5 cut(s) 163, 232, 358, 363, 591
EaeI YGGCCR 2 cut(s) 424, 651
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 421, 511, 530
Eco147I AGGCCT 1 cut(s) 443
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 105, 212
Eco88I CYCGRG 1 cut(s) 299
EcoRI GAATTC 2 cut(s) 75, 625
EcoRII CCWGG 1 cut(s) 101
EcoRV GATATC 1 cut(s) 346
EcoT14I CCWWGG 3 cut(s) 421, 511, 530
ErhI CCWWGG 3 cut(s) 421, 511, 530
FaeI CATG 3 cut(s) 425, 515, 520
FaqI GGGAC 1 cut(s) 512
FatI CATG 3 cut(s) 421, 511, 516
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 473
GlaI GCGC 2 cut(s) 124, 151
GluI GCNGC 1 cut(s) 473
HaeIII GGCC 3 cut(s) 426, 443, 653
HapII CCGG 3 cut(s) 23, 126, 200
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 125, 152
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 3 cut(s) 425, 515, 520
Hin6I GCGC 2 cut(s) 123, 150
HinP1I GCGC 2 cut(s) 123, 150
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 3 cut(s) 274, 280, 525
HpaII CCGG 3 cut(s) 23, 126, 200
HphI GGTGA 2 cut(s) 12, 214
Hpy166II GTNNAC 2 cut(s) 108, 454
Hpy188I TCNGA 9 cut(s) 81, 157, 168, 216, 279, 285, 314, 363, 565
Hpy188III TCNNGA 2 cut(s) 236, 299
Hpy8I GTNNAC 2 cut(s) 108, 454
Hpy99I CGWCG 2 cut(s) 85, 157
HpyAV CCTTC 1 cut(s) 696
HpyCH4III ACNGT 3 cut(s) 62, 112, 140
HpyCH4IV ACGT 2 cut(s) 415, 456
HpyCH4V TGCA 2 cut(s) 143, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 3 cut(s) 284, 320, 562
HpySE526I ACGT 2 cut(s) 415, 456
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 3 cut(s) 425, 515, 520
HspAI GCGC 2 cut(s) 123, 150
Kzo9I GATC 5 cut(s) 163, 232, 358, 363, 591
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 1 cut(s) 610
MaeII ACGT 2 cut(s) 415, 456
MaeIII GTNAC 1 cut(s) 18
MalI GATC 5 cut(s) 165, 234, 360, 365, 593
MboI GATC 5 cut(s) 163, 232, 358, 363, 591
MboII GAAGA 4 cut(s) 31, 242, 601, 723
MflI RGATCY 2 cut(s) 358, 591
MhlI GDGCHC 1 cut(s) 686
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 5 cut(s) 75, 158, 169, 388, 625
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 274, 534
MmeI TCCRAC 2 cut(s) 104, 135
MnlI CCTC 7 cut(s) 234, 279, 283, 335, 571, 642, 732
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 2 cut(s) 132, 173
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 3 cut(s) 23, 126, 200
MspR9I CCNGG 2 cut(s) 103, 127
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 149
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 2 cut(s) 421, 511
NdeII GATC 5 cut(s) 163, 232, 358, 363, 591
NlaIII CATG 3 cut(s) 425, 515, 520
NlaIV GGNNCC 4 cut(s) 198, 213, 559, 683
NmeAIII GCCGAG 2 cut(s) 173, 246
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 73
PaeI GCATGC 1 cut(s) 520
PceI AGGCCT 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 274
Pfl23II CGTACG 1 cut(s) 180
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 2 cut(s) 274, 533
PpsI GAGTC 2 cut(s) 274, 533
PsiI TTATAA 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 4 cut(s) 198, 213, 559, 683
PspPI GGNCC 2 cut(s) 105, 212
PsrI GAACNNNNNNTAC 2 cut(s) 173, 205
PsuI RGATCY 2 cut(s) 358, 591
RsaI GTAC 3 cut(s) 182, 251, 569
RsaNI GTAC 3 cut(s) 181, 250, 568
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 132, 173
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 5 cut(s) 163, 232, 358, 363, 591
Sau96I GGNCC 2 cut(s) 105, 212
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 2 cut(s) 274, 534
ScrFI CCNGG 2 cut(s) 103, 127
SduI GDGCHC 1 cut(s) 686
SetI ASST 8 cut(s) 107, 179, 306, 321, 374, 418, 459, 563
SfaNI GCATC 1 cut(s) 610
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 2 cut(s) 105, 212
SphI GCATGC 1 cut(s) 520
Sse9I AATT 5 cut(s) 75, 158, 169, 388, 625
SseBI AGGCCT 1 cut(s) 443
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 2 cut(s) 101, 125
StyI CCWWGG 3 cut(s) 421, 511, 530
TaaI ACNGT 3 cut(s) 62, 112, 140
TaiI ACGT 2 cut(s) 418, 459
TaqI TCGA 5 cut(s) 73, 95, 327, 547, 609
TasI AATT 5 cut(s) 75, 158, 169, 388, 625
TatI WGTACW 2 cut(s) 249, 567
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 132, 173
Tru9I TTAA 2 cut(s) 132, 173
TscAI CASTG 2 cut(s) 145, 150
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 2 cut(s) 145, 150
VpaK11BI GGWCC 2 cut(s) 105, 212
XapI RAATTY 3 cut(s) 75, 169, 625
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 528
ZraI GACGTC 1 cut(s) 457
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.